LOCUS       sequence-182182-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEV::linker::tagBFP::3XFlag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP975-1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..36
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             88..783
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     CDS             784..849
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cggtacccgg ggatcgagaa cctctacttc caaggatgcc cgggggatcg gtggagctcc
       61 accggtggcg gccgctctag aactagtagc gagctgatta aggagaacat gcacatgaag
      121 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga aggcgaaggc
      181 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
      241 ttcgccttcg acatcctggc gactagcttc ctctacggca gcaagacctt catcaaccac
      301 acccagggca tccccgactt cttcaagcag tccttccctg aaggcttcac atgggagaga
      361 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
      421 ggctgcctca tatacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
      481 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaaa cgctgtaccc cgctgacggc
      541 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
      601 aacatcaaaa ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
      661 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
      721 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
      781 aatgactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
      841 gatgacaagg atcctctaga gtcg
//
