LOCUS       sequence-182183-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEV::linker::tagRFP::3XFlag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP974-1
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..36
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             88..795
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             796..861
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cggtacccgg ggatcgagaa cctctacttc caaggatgcc cgggggatcg gtggagctcc
       61 accggtggcg gccgctctag aactagtgtg tctaagggcg aagagctgat taaggagaac
      121 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa atgcacatcc
      181 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
      241 ggccctctcc ccttcgcctt tgacatcctg gctaccagct tcatgtacgg cagcagaacc
      301 ttcatcaacc acacccaggg catccccgac ttctttaagc agtcctttcc tgagggcttc
      361 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
      421 agcctccagg acggctgtct catctacaac gtcaagatca gaggggtgaa cttcccatcc
      481 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccaataccga gatgctgtac
      541 cccgctgacg gcggcctgga aggcagaagc gacatggccc tgaagctcgt gggcgggggc
      601 cacctgatct gcaatttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag
      661 atgcccggcg tctactatgt ggaccacaga ctggaaagaa tcaaagaggc cgacaaagag
      721 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
      781 gggcacaaac ttaatgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
      841 tacaaggatg acgatgacaa ggatcctcta gagtcg
//
