LOCUS       sequence-182208-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEV::linker::meGFP:3XFlag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP973-1
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..36
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(118..146)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     741..761
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             802..867
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cggtacccgg ggatcgagaa cctctacttc caaggatgcc cgggggatcg gtggagctcc
       61 accggtggcg gccgctctag aactagtgtg agtaaaggag aagaactttt cactggagtt
      121 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      181 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
      241 aaactacctg ttccatggcc aacacttgtc actactctga cctacggcgt gcagtgcttc
      301 agccggtacc ccgaccacat gaagcagcat gactttttca agagtgccat gcccgaaggt
      361 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
      421 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
      481 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
      541 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
      601 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      661 cctgtccttt taccagacaa ccattacctg tccacacaat ctaagctttc gaaagatccc
      721 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacactc
      781 ggcatggacg agctgtacaa ggactacaaa gaccatgacg gtgattataa agatcatgat
      841 atcgattaca aggatgacga tgacaaggat cctctagagt cg
//
