LOCUS       sequence-182211-        7878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7878)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-PTK2-C-10
FEATURES             Location/Qualifiers
     source          1..7878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    3295..3416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3332..3351)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3386..3405
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3423..3878)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3560..3581)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3772..3791
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3905..4009
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4011..4368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4011..4202
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4034..4054)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4219..4354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4403..5197
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4403..5197
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4457..4476)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5067..5086
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5385..5404)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5429..5476
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5805..6393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6294..6313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6568..6871
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6872..7075
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7026..7046
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             7120..7797
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gcagctgctt accttgaccc caacttgaat cacacaccaa attcgagtac taagactcac
       61 ctgggtactg gtatggaacg ttctcctggt gcaatggagc gagtattaaa ggtctttcat
      121 tattttgaaa gcaatagtga gccaaccacc tgggccagta ttatcaggca tggagatgct
      181 actgatgtca ggggcatcat tcagaagata gtggacagtc acaaagtaaa gcatgtggcc
      241 tgctatggat tccgcctcag tcacctgcgg tcagaggagg ttcactggct tcacgtggat
      301 atgggcgtct ccagtgtgag ggagaagtat gagcttgctc acccaccaga ggagtggaaa
      361 tatgaattga gaattcgtta tttgccaaaa ggatttctaa accagtttac tgaagataag
      421 ccaactttga atttcttcta tcaacaggtg aagagcgatt atatgttaga gatagctgat
      481 caagtggacc aggaaattgc tttgaagttg ggttgtctag aaatacggcg atcatactgg
      541 gagatgcggg gcaatgcact agaaaagaag tctaactatg aagtattaga aaaagatgtt
      601 ggtttaaagc gattttttcc taagagttta ctggattctg tcaaggccaa aacactaaga
      661 aaactgatcc aacaaacatt tagacaattt gccaacctta atagagaaga aagtattctg
      721 aaattctttg agatcctgtc tccagtctac agatttgata aggaatgctt caagtgtgct
      781 cttggttcaa gctggattat ttcagtggaa ctggcaatcg gcccagaaga aggaatcagt
      841 tacctaacgg acaagggctg caatcccaca catcttgctg acttcactca agtgcaaacc
      901 attcagtatt caaacagtga agacaaggac agaaaaggaa tgctacaact aaaaatagca
      961 ggtgcacccg agcctctgac agtgacggca ccatccctaa ccattgcgga gaatatggct
     1021 gacctaatag atgggtactg ccggctggtg aatggaacct cgcagtcatt tatcatcaga
     1081 cctcagaaag aaggtgaacg ggctttgcca tcaataccaa agttggccaa cagcgaaaag
     1141 caaggcatgc ggacacacgc cgtctctgtg tcagaaacag atgattatgc tgagattata
     1201 gatgaagaag atacttacac catgccctca accagggatt atgagattca aagagaaaga
     1261 atagaacttg gacgatgtat tggagaaggc caatttggag atgtacatca aggcatttat
     1321 atgagtccag agaatccagc tttggcggtt gcaattaaaa catgtaaaaa ctgtacttcg
     1381 gacagcgtga gagagaaatt tcttcaagaa gccttaacaa tgcgtcagtt tgaccatcct
     1441 catattgtga agctgattgg agtcatcaca gagaatcctg tctggataat catggagctg
     1501 tgcacacttg gagagctgag gtcatttttg caagtaagga aatacagttt ggatctagca
     1561 tctttgatcc tgtatgccta tcagcttagt acagctcttg catatctaga gagcaaaaga
     1621 tttgtacaca gggacattgc tgctcggaat gttctggtgt cctcaaatga ttgtgtaaaa
     1681 ttaggagact ttggattatc ccgatatatg gaagatagta cttactacaa agcttccaaa
     1741 ggaaaattgc ctattaaatg gatggctcca gagtcaatca attttcgacg ttttacctca
     1801 gctagtgacg tatggatgtt tggtgtgtgt atgtgggaga tactgatgca tggtgtgaag
     1861 ccttttcaag gagtgaagaa caatgatgta atcggtcgaa ttgaaaatgg ggaaagatta
     1921 ccaatgcctc caaattgtcc tcctaccctc tacagcctta tgacgaaatg ctgggcctat
     1981 gaccccagca ggcggcccag gtttactgaa cttaaagctc agctcagcac aatcctggag
     2041 gaagagaagg ctcagcaaga agagcgcatg aggatggagt ccagaagaca ggccacagtg
     2101 tcctgggact ccggagggtc tgatgaagca ccgcccaagc ccagcagacc gggttatccc
     2161 agtccgaggt ccagcgaagg attttatccc agcccacagc acatggtaca aaccaatcat
     2221 taccaggttt ctggctaccc tggttcacat ggaatcacag ccatggctgg cagcatctat
     2281 ccaggtcagg catctctttt ggaccaaaca gattcatgga atcatagacc tcaggagata
     2341 gcaatgtggc agcccaatgt ggaggactct acagtattgg acctgcgagg gattgggcaa
     2401 gtgttgccaa cccatctgat ggaagagcgt ctaatccgac agcaacagga aatggaagaa
     2461 gatcagcgct ggctggaaaa agaggaaaga tttctgaaac ctgatgtgag actctctcga
     2521 ggcagtattg acagggagga tggaagtctt cagggtccga ttggaaacca acatatatat
     2581 cagcctgtgg gtaaaccaga tcctgcagct ccaccaaaga aaccgcctcg ccctggagct
     2641 cccggtcatc tgggaagcct tgccagcctc agcagccctg ctgacagcta caacgagggt
     2701 gtcaagccat ggaggcttca gccccaggaa atcagccccc ctcctactgc caacctggac
     2761 cggtcgaatg ataaggtgta cgagaatgtg acgggcctgg tgaaagctgt catcgagatg
     2821 tccagtaaaa tccagccagc cccaccagag gagtatgtcc ctatggtgaa ggaagtcggc
     2881 ttggccctga ggacattatt ggccactgtg gatgagacca ttcccctcct accagccagc
     2941 acccaccgag agattgagat ggcacagaag ctattgaact ctgacctggg tgagctcatc
     3001 aacaagatga aactggccca gcagtatgtc atgaccagcc tccagcaaga gtacaaaaag
     3061 caaatgctga ctgctgctca cgccctggct gtggatgcca aaaacttact cgatgtcatt
     3121 gaccaagcaa gactgaaaat gcttgggcag acgagaccac actgaggatc caccggatct
     3181 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     3241 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     3301 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3361 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     3421 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     3481 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     3541 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     3601 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     3661 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     3721 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     3781 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     3841 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3901 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3961 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     4021 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     4201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4321 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     4381 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     4441 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     4501 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     4561 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     4621 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     4681 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     4741 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     4801 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     4861 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4921 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4981 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5041 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5101 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     5161 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     5221 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     5281 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     5341 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     5401 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     5461 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     5521 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     5581 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     5641 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5701 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5761 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5821 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5881 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5941 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6001 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6061 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6481 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     6541 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     6601 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     6661 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     6721 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     6781 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     6841 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     6901 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     6961 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     7021 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
     7081 agtgaaccgt cagatccgct agcgctaccg gtcgccacca tgagtgcgat taagccagac
     7141 atgaagatca acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga
     7201 gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc
     7261 ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta
     7321 ttcgccgaat atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat
     7381 tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata
     7441 acaatggaag gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc
     7501 aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat
     7561 gtgcgtgatg gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc
     7621 cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca
     7681 ggctaccact ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag
     7741 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc
     7801 ggactcagat ctcgagctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
     7861 tctagcggtg gcagtgga
//
