LOCUS       sequence-182221-       11143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cell death reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11143)
  AUTHORS   Morsch M, Radford R, Lee A, Don EK, Badrock AP, Hall TE, Cole NJ, 
            Chung R
  TITLE     In vivo characterization of microglial engulfment of dying neurons 
            in the zebrafish spinal cord.
  JOURNAL   Front Cell Neurosci. 2015 Aug 31;9:321. doi: 
            10.3389/fncel.2015.00321. eCollection 2015.
  PUBMED    26379496
REFERENCE   2  (bases 1 to 11143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Neurosci."; date: "2015-08-31"; volume: "9:321. doi"; pages: " 
            10.3389/fncel.2015.00321. eCollection 2015"
COMMENT     Sequence Label: pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP
FEATURES             Location/Qualifiers
     source          1..11143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    138..158
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3693..3717
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3718..3727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4739..4763)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(4765..4767,4768..5481)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4807..4828)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5068..5087)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5415..5436
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5483..5503)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(5511..5527)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5543..5677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(6116..6303,6304..6315)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(6349..6365)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6349..6365)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6402..6422)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6402..6420)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6441..6457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6454..6476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6465..6481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6489..6495,6496..6513,6514..6519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6534..6555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6672..6689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6843..7431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6923..6942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7602..8393,8394..8462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8225..8244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8463..8567)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8730..8751)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(9100..9111,9112..9274)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    9643..9764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(9887..10597)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(9967..9990)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(10165..10184)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10434..10452
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      10594..10603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 taacatcctg cactgaatgc acactattaa tagaaaagag agcattattg taggaaatgt
       61 attgtatatg tacattctgt gcatttcaca attttctcga gcaggaaaca gctatgacca
      121 tgattacgcc aagctatcaa ctttgtatag aaaagttggc tccgaattcg cccttaaaac
      181 tcgagaccag caaagttcta gaatttgtcg aaacatttat gttatatatt tcctgaaaaa
      241 aattctgagt aagttcttaa gtgttattgc cagcaacata aacaacagac ggcaaaatga
      301 ataaatgata acaaagcagt aggcttaaat aaacctaatt tttataggct gttctctaca
      361 accctcaaac agtgattagt tttgtactta taaacttgcc ctttcattca tatttcaaga
      421 aaattggttc agaagatctg gatattctag cagttgttca agctcatgga gggatcagtg
      481 acctgattcc acaatgacta ggcctaatcc agaaattaga tgactgtcaa cataaaaagg
      541 cacagcactc actagctgcc ctatatattt tattatattt tacatatatt attttattta
      601 tttagctctg agtgctgtac tttctggtta aagaaaactg cttacaacag ctaacctgta
      661 ctacctcagg ctcagggaat ttggaacagg tttgtctggt ttgtttcttt aaccatgcat
      721 gcttgttttc aactatggca acacagtcac atgggacatt acagaaatga tttgtcgatg
      781 acatgcgact tttctttaat aaagcgcaaa gatcccaaaa agcaaacttt taacaaaaat
      841 catataatta tattttcaat ccagctttgt agcaactttg tgctgctgtt cactcagcaa
      901 cagatagtca gtataaggtc agtgtgtctc aaagcagtgc catctgtttc acacattgcg
      961 ttctatatat aagtgtgctg gttgacacga cactgtataa ggcctaggct aaaacacaaa
     1021 caatgtagaa tgacactgtg ttttttttgt aaacaaatgt tgtttttggt taaacatctt
     1081 tgtgaaaaca tcctcctgtc atgtatttgc tatattcaaa tgttaaaccc gtgcagaata
     1141 gaacatatac aaaaaaaaac aacacaacac atttttaaac attattaaat atcaagtatt
     1201 gctggcagtt ctgtttctgt tttacagtac cctttgccac agttctccgc ttttcctggt
     1261 ccagattcca caagtctgat tcaccaatag caaagcgaat aaacaaccaa agcagccaat
     1321 cactgcttgt agactgtcct gcgagaccgg cccattccag cacattctgg aaacttcctt
     1381 tatatgataa ttataaatac atttaaatta ttgatacaaa acatgtaatt cctagaacat
     1441 aaccatagca atcattagtt ttcagggtaa ttatgtattt ttaggatttg actgcggaaa
     1501 gatctggtca tgtgacgtct catgaacgtc acggccctgg gtttctataa atacagtagg
     1561 actctcgacc atcggcagat ttttcgaaga agaagatcag tttcaggagc cgtactgttc
     1621 cgtttcaacg caaatattaa cggtaagagc gaatttccta gtttgttttc atgccattct
     1681 ttaaaaccat agcgtattac tttaattata gtaaactttc gctttcttta ttacaagaga
     1741 cgttttgtgt tgattctccg cggacatttt cggtcagaca atcagaaaat gaccgcggag
     1801 gaccagtaac ttgcattaca cgtaagttaa atcttcgtgt attaaaatgg ttaggttgtt
     1861 aacgtcaaat aggttaccgt gtttgcgtgt gatcaggttg gttttgttag atttttgtca
     1921 gtatttttaa tttatttgtt ttagtttatt tatttttttt gctgaatcat agtttgtgaa
     1981 caaagaaccc ggatgttaca tacagtacag ccgccatgtt acagagagtt ataacttaat
     2041 cattttaaaa ataattttgc cttactttta gtttgtcatg ttgagaaatg aggaaatgtt
     2101 aaaatgagga aatatccaat taatttaata tatcaaaata atccatgatt acaatgcact
     2161 gaactggaga aaattaagat gttttctagt gtcatgaaac aaatgtaaga gatgtacatt
     2221 gtagatgttt tatgtcaaga attggctagt tgatgcagca tactggcgat actcagttgt
     2281 aataacagta acgttacatg ttaatagact actgagtatg ctgttctgtc tatgtatgct
     2341 ctgtaagcta cgagaaggac ttttttaaac agtaaagggt gcaatatttt tacaaattga
     2401 attaaataaa ggctgtctat taagtaatat gcttgatatt tttcttactt gatcggaaat
     2461 aagaaaaaat ataaacgttg ttgctctaaa aatcctagtt cagtttagcc aaccacaaat
     2521 acctttttgt tcctccaaca gttttttttt cttctctata atatttggca gtctatagta
     2581 ctccaaatgt ttccccacag tctaactaat tggtacagcc aaaatcatga cacttattgc
     2641 aataataatt ttggttcatt ggcattgttg atagcctgtg ccactaatat ggtcgattga
     2701 tcatgcttca ggaagaaaac tatattgttt gatgtaagat tattaaatct tcacctgctt
     2761 ccattacaaa ctattcccat cttattgaat tctggtatgt cttaaaggat tagttcactt
     2821 cccaaatcaa aatttactta gccttttttc atccatgatc cctttttttc atcattaatg
     2881 aagaaattgt ttttgaaaaa gtttcaagat ttttttctct atattgtgga gcttgttagt
     2941 ttaaaattcc aaaatgcaat atgtggcttc aaatggttct aaatgatccc agtcaaggaa
     3001 taacagtctt atctaatgaa accattagac ctttaaaaat aaaaaaaata aaagtattta
     3061 tttttaaatg actgagtgat taagttgaat ttcagcgttt ccttactgtg tagaagtcct
     3121 tccttactgg ccccaccctt tggttctctg ccaatctgct acctaatgta atgttgtgga
     3181 acattattat tctttatttc ttaatttttt attttttatt ttaaaacaat gtaaactgca
     3241 cagatgtgca gtttgtttaa aatggccaat gctttggaaa tgcatgacat aattagattt
     3301 catgatgcac aaagccaaat ctcagagctt gtgcaaaatg agctatcatt tcactaggta
     3361 agaccctaaa ttttcatata ggatcatttg gacaattttg ctgcaggtaa aatgcattct
     3421 atagtccact gtcagccatt gttttggata gtatttattt ttctctacaa gtatagtcaa
     3481 tagttttcta ttattttaaa ggtttgtaac atttaagggt gaccaaatgc aaagtaaaat
     3541 ttcattttcg ggtgaactat ctcgtttaac atgggagaag tgcaaaacat acattattgg
     3601 ctagaacatt gtagtatttt ttaaatggaa atgtgtgatt gctaatctta ctttgaattt
     3661 gtttacaggg atcctttaag ggcgaattcg acccaagttt gtacaaaaaa gcaggctgcc
     3721 accatggtgc acaaggtgct gctggccctg ttcttcatct tcctggcccc cgccggcacc
     3781 gcacaggttc tcagaggcac tgtgactgac ttccctggat ttgatgagcg ggctgatgca
     3841 gaaactcttc ggaaggctat gaaaggcttg ggcacagatg aggagagcat cctgactctg
     3901 ttgacatccc gaagtaatgc tcagcgccag gaaatctctg cagcttttaa gactctgttt
     3961 ggcagggatc ttctggatga cctgaaatca gaactaactg gaaaatttga aaaattaatt
     4021 gtggctctga tgaaaccctc tcggctttat gatgcttatg aactgaaaca tgccttgaag
     4081 ggagctggaa caaatgaaaa agtactgaca gaaattattg cttcaaggac acctgaagaa
     4141 ctgagagcca tcaaacaagt ttatgaagaa gaatatggct caagcctgga agatgacgtg
     4201 gtgggggaca cttcagggta ctaccagcgg atgttggtgg ttctccttca ggctaacaga
     4261 gaccctgatg ctggaattga tgaagctcaa gttgaacaag atgctcaggc tttatttcag
     4321 gctggagaac ttaaatgggg gacagatgaa gaaaagttta tcaccatctt tggaacacga
     4381 agtgtgtctc atttgagaaa ggtgtttgac aagtacatga ctatatcagg atttcaaatt
     4441 gaggaaacca ttgaccgcga gacttctggc aatttagagc aactactcct tgctgttgtg
     4501 aaatctattc gaagtatacc tgcctacctt gcagagaccc tctattatgc tatgaaggga
     4561 gctgggacag atgatcatac cctcatcaga gtcatggttt ccaggagtga gattgatctg
     4621 tttaacatca ggaaggagtt taggaagaat tttgccacct ctctttattc catgattaag
     4681 ggagatacat ctggggacta taagaaagct cttctgctgc tctgtggaga agatgactac
     4741 ccagctttct tgtacaaagt ggctgtgagc aagggcgagg agctgttcac cggggtggtg
     4801 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4861 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     4921 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     4981 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5041 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5101 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5161 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5221 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     5281 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5341 gtgctgctgc ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac
     5401 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5461 atggacgagc tgtacaagta accaacttta ttatacatag ttgataattc actggccgtc
     5521 gttttacggt accatcgatg atgatccaga catgataaga tacattgatg agtttggaca
     5581 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5641 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     5701 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     5761 atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg ccacgggtgc
     5821 tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga atcacatact
     5881 tcctccattt tcttccactg attgactgtt ataatttccc taatttccag gtcaaggtgc
     5941 tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat gaacatgtct
     6001 gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac atttaaatta
     6061 aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata gaatgaagtg
     6121 atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta aaaagtactt
     6181 ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa
     6241 gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa attactcaag
     6301 tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga tccactagtt
     6361 ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt
     6421 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6481 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6541 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6601 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6661 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7261 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta aggcattttg
     7501 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8281 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8341 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8401 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8461 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8521 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8581 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     8641 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     8701 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     8761 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccacgctggc
     8821 taagaactca tcagcctccc cggtccatct acccacgtac caatgcacca attggccaca
     8881 atgacggcta ctacatggtg ccattccttc ctctttatag gaatggagac tacctcctgt
     8941 ccaacaatgc tcttggatac gagtacgcct acctgttgga cccaggtcat tgcacaacac
     9001 cagaaatgcc ctctgatctg caaaagacgt gaatatctgt tcagacaccc atatccactc
     9061 tgttccacac aggtcagagg tttgtccagg agttcttgac agaggtgtaa aaagtactca
     9121 aaaattttac tcaagtgaaa gtacaagtac ttagggaaaa ttttactcaa ttaaaagtaa
     9181 aagtatctgg ctagaatctt acttgagtaa aagtaaaaaa gtactccatt aaaattgtac
     9241 ttgagtatta aggaagtaaa agtaaaagca agaaagaaaa ctagagattc ttgtttaagc
     9301 ttttaatctc aaaaaacatt aaatgaaatg catacaaggt tttatcctgc tttagaactg
     9361 tttgtattta attatcaaac tataagacag acaatctaat gccagtacac gctactcaaa
     9421 gttgtaaaac ctcagattta acttcagtag aagctgattc tcaaaattgt tagtgtcaag
     9481 cctagctctt ttggggctga aaagcaatcc tgcagtgctg aaaagcctct cacaggcagc
     9541 cgatgcggga agaggtgtat tagtcttgat agagaggctg caaatagcag gaaacgtgag
     9601 cagagactcc ctggtgtctg aaacacaggc ccatttacgc cttaagatac attgatgagt
     9661 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     9721 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     9781 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     9841 tctacaaatg tggtatggct gattatgatc tagagtcgcg gccgctttac ttgtacagct
     9901 cgtccatgcc gccggtggag tggtggccct cggcgcgctc gtactgttcc acgatggtgt
     9961 agtcctcgtt gtgggaggtg atgtccagct tgatgttgac gttgtaggcg ccgggcagct
    10021 gcacgggctt cttggccttg taggtggtct tgacctcggc gtcgtagtgg ccgccgtcct
    10081 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
    10141 cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt
    10201 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
    10261 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
    10321 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
    10381 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
    10441 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
    10501 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
    10561 tgatggccat gttgtcatcc tcgcccttgc tcaccatggt ggcgaccggt aatgtcagac
    10621 ctggtaactc cttactgtaa cttattgcac ctgatgaaca aaagatgtga actgaacgca
    10681 agtaggaggc taatagaggc tagtacagta caaagatccc caaatgatgt gtgccggcct
    10741 tagtggaggc agacccctgg ttatatacct gtgctgcaga aagaggggct tacaaatgga
    10801 tctctctgga atggcattat ctcatggtgg gaacagtgtt gtcagcagaa tcagcggggt
    10861 ggactctgac ctgagaatga cagtctagca ctgaatggct cagacaatac tcttgtggcc
    10921 aactatcacc ggaaaataag tgcctgccaa aacaaaccag accagatgaa gctacatggc
    10981 tgtagaacgt cagtatttaa tgtgttgtgg ccacttggtt caccagattt aagagctaat
    11041 ttttttaaat atcacagata atcaatcgct tttttttttc ttgactgaaa agcttagact
    11101 ggaaaattct ttgaaaaagt ttgcactaca gaaaataaat aca
//
