LOCUS       sequence-182223-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  nirFP::3xFlag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP693-3
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             715..780
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cggtacccgg ggatcggcga agatagtgag ctcattagtg aaaacatgca cacaaaactc
       61 tatatggaag gaacagtgaa cggtcatcac tttaaatgta cctcagaggg agaaggaaaa
      121 ccatatgaag gcacacagac ctgcaaaatt aaagttgtgg aaggaggacc actgccgttt
      181 gcctttgata ttttggcgac aagttttatg tatggaagta aaacgtttat taatcacact
      241 caaggcatcc cagacttctt taaacagtct tttccagaag gtttcacgtg ggaaagaatc
      301 actacttatg aggatggagg cgttcttacg gctacacaag atactagtct tcaaaatgga
      361 tgcctgatat acaacgtgaa aatcaatgga gtcaattttc cttccaacgg acctgtgatg
      421 caaaagaaaa cactgggctg ggaggcaaac acggaaatgc tctatcctgc cgattcaggt
      481 ctgcgaggac acaaccagat ggccctcaaa ttggtcggag gaggttatct gcattgttca
      541 ctcaagacaa cataccgatc taagaaacct gcgaagaatt tgaaaatgcc gggcttttac
      601 tttgttgatc gaaagctcga gagaattaaa gaggctgata aagaaacgta tgtggaacaa
      661 cacgaaatgg ctgtggcacg atactgtgat ttgccatcta aactcggtca ttcagactac
      721 aaggatcacg atggagatta caaagatcac gacatcgact ataaggacga tgatgataaa
      781 gatcctctag agtcg
//
