LOCUS       sequence-182230-         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3XFlag::TEV::V5::mOrange2::V5::TEV::3XFlag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP640-2
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             103..144
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             850..891
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             892..912
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             913..978
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cggtacccgg ggatcgatta taaagatcat gatggggatt ataaagatca tgatatagat
       61 tataaagatg atgatgataa agaaaattta tattttcagg gtggtaagcc tatccctaac
      121 cctctcctcg gtctagatag tactgtctcc aagggagagg agaataacat ggccatcatc
      181 aaggagttca tgcgtttcaa ggtccgtatg gagggatccg tcaacggaca cgagttcgag
      241 atcgagggag agggcgaggg acgtccatac gagggattcc agaccgccaa gctcaaggtc
      301 accaagggag gacccctccc attcgcctgg gacatcctgt ccccacactt cacctacggc
      361 tccaaggcct acgtcaagca cccagccgac atcccagact acttcaagct ctccttcccc
      421 gagggattca agtgggagcg tgtcatgaac tacgaggacg gaggagtggt caccgtcacc
      481 caagactcct ccctccaaga cggagagttc atctacaagg tgaagctccg tggaaccaac
      541 ttcccatccg acggcccagt catgcaaaag aagaccatgg gatgggaggc ctcctccgag
      601 cggatgtacc cagaggacgg agccctgaag ggaaagatca agatgaggct caagctcaag
      661 gacggaggac actacacctc cgaggtcaag accacctaca aggccaagaa gccagtccaa
      721 ctcccaggag cctacatcgt cgacatcaag ctcgacatca cctcccacaa cgaggactac
      781 accatcgtgg agcaatacga acgtgccgag ggacgccact ccaccggagg aatggacgag
      841 ctctacaagg gaaagccaat cccaaaccca ctcctcggac tcgactccac cgagaacctc
      901 tacttccaag gagactacaa agaccatgac ggtgattata aagatcatga tatcgattac
      961 aaggatgacg atgacaagga tcctctagag tcg
//
