LOCUS       sequence-182234-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myc::TEV::3XFlag::Dendra2::3XFlag::TEV::Myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP635-2
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..45
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             67..132
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             820..885
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             886..906
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             907..936
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cggtacccgg ggatcgagca aaagctcatc tccgaggagg acctcgaaaa tttatatttt
       61 cagggtgact acaaggacca cgacggagac tacaaggacc acgacatcga ctacaaggac
      121 gacgacgaca agaacacccc aggaatcaac ctcatcaagg aggacatgcg tgtcaaggtc
      181 cacatggagg gaaacgtcaa cggacacgcc ttcgtcatcg agggagaggg aaagggaaag
      241 ccatacgagg gaacccaaac cgccaacctc accgtcaagg agggagcccc actcccattc
      301 tcctacgaca tcctcaccac cgccgtccac tacggaaacc gtgtcttcac caagtaccca
      361 gaggacatcc cagactactt caagcaatcc ttcccagagg gatactcctg ggagcgtacc
      421 atgaccttcg aggacaaggg aatctgcacc atccgttccg acatctccct cgagggagac
      481 tgcttcttcc aaaacgtccg tttcaaggga accaacttcc caccaaacgg accagtcatg
      541 caaaagaaga ccctcaagtg ggagccatcc accgagaagc tccacgtccg tgacggactc
      601 ctcgtcggaa acatcaacat ggccctcctc ctcgagggag gaggacacta cctctgcgac
      661 ttcaagacca cctacaaggc caagaaggtc gtccaactcc cagacgccca cttcgtcgac
      721 caccgtatcg agatcctcgg aaacgactcc gactacaaca aggtcaagct ctacgagcac
      781 gccgtcgccc gttactcccc actcccatcc caagtctggg actacaaaga ccatgacggt
      841 gattataaag atcatgatat cgattacaag gatgacgatg acaaggagaa cctctacttc
      901 caaggagaac aaaaactgat atcagaagag gatctggatc ctctagagtc g
//
