LOCUS       sequence-182235-        1772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP608-1
FEATURES             Location/Qualifiers
     source          1..1772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(463..491)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1239..1259
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1300..1365
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1366..1386
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1387..1416
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      1431..1754
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
ORIGIN
        1 cggtacccgg ggatcatgcc accaaagcca tctgccaagg gagccaagaa ggccgccaag
       61 accgtcgttg ccaagccaaa ggacggaaag aagagacgtc atgcccgcaa ggaatcgtac
      121 tccgtctaca tctaccgtgt tctcaagcaa gttcacccag acaccggagt ctcctccaag
      181 gccatgtcta tcatgaactc cttcgtcaac gatgtattcg aacgcatcgc ttcggaagct
      241 tcccgtcttg ctcattacaa caaacgctca acgatctcat cccgcgaaat tcaaaccgct
      301 gtccgtttga ttctcccagg agaacttgcc aagcacgccg tgtctgaggg aaccaaggcc
      361 gtcaccaagt acacttccag caagtgcccg ggggatcggt ggagctccac cggtggcggc
      421 cgctctagaa ctagtagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      481 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      541 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      601 tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca
      661 cttgtcacta ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa
      721 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      781 ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca
      841 tacatattta aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      901 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      961 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
     1021 gttgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc
     1081 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     1141 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     1201 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     1261 gctgctggga ttacacatgg catggacgaa ctatacaaag actacaaaga ccatgacggt
     1321 gattataaag atcatgatat cgattacaag gatgacgatg acaaggagaa cctctacttc
     1381 caaggagaac aaaaactgat atcagaagag gatctgtaaa ctagtgataa atgcaaaatc
     1441 ctttcaagca ttcccttctt ctctatcact cttctttctt tttgtcaaaa aattctctcg
     1501 ctaatttatt tgctttttta atgttattat tttatgactt tttatagtca ctgaaaagtt
     1561 tgcatctgag tgaagtgaat gctatcaaaa tgtgattctg tctgatgtac tttcacaatc
     1621 tctcttcaat tccattttga agtgctttaa acccgaaagg ttgagaaaaa tgcgagcgct
     1681 caaatatttg tattgtgttc gttgagtgac ccaacaaaaa gaggaaactt tattgtgccg
     1741 ccaagaaaaa agtctcagat cctctagagt cg
//
