LOCUS       sequence-182236-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP607-2
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..45
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             67..132
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             133..843
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(160..188)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     783..803
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             844..909
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             910..930
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             931..960
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cggtacccgg ggatcgagca aaagctcatc tccgaggagg acctcgaaaa tttatatttt
       61 cagggtgact acaaggacca cgacggagac tacaaggacc acgacatcga ctacaaggac
      121 gacgacgaca agagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      181 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      241 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      301 ccaacacttg tcactacttt ctgttatggt gttcaatgct tctcgagata cccagatcat
      361 atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
      421 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
      481 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
      541 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
      601 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
      661 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
      721 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
      781 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga cgaactatac
      841 aaagactaca aagaccatga cggtgattat aaagatcatg atatcgatta caaggatgac
      901 gatgacaagg agaacctcta cttccaagga gaacaaaaac tgatatcaga agaggatctg
      961 gatcctctag agtcg
//
