LOCUS       sequence-182239-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3XFlag::Topaz::Myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP574-1
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(82..84,85..795)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(124..145)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(385..404)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     732..753
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             796..825
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cggtacccgg ggatcgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
       61 tacaaggatg acgatgacaa ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      121 atactggtcg agctggacgg cgacgtaaac ggccacaagt tcagtgtgtc cggcgagggc
      181 gagggcgatg ccacctacgg caagctaacc ctgaagttca tctgcaccac cggcaagctg
      241 cccgtgccct ggcccacgct cgtgaccacc ttcggctacg gcgtgcagtg cttcgcccgc
      301 taccccgacc acatgcgcca gcacgacttc ttcaagtccg ccatgcccga aggctatgtc
      361 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
      421 ttcgagggcg acaccctagt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
      481 ggcaacatcc tggggcacaa gctggagtac aactataaca gccacaacgt ctatatcatg
      541 gccgacaagc agaagaacgg cataaaggtg aacttcaaga tccgccacaa catcgaggac
      601 gggagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
      661 ctgctgcccg acaaccacta cctgagctac cagtccgccc taagcaaaga ccccaacgag
      721 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
      781 gacgagctgt acaaggaaca aaaactgata tcagaagagg atctggatcc tctagagtcg
//
