LOCUS       sequence-182240-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3XFlag::mNeptune::Myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP546-2
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             811..840
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cggtacccgg ggatcgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
       61 tacaaggatg acgatgacaa ggtctccaag ggagaggagc tcatcaagga gaacatgcac
      121 atgaagctct acatggaggg aaccgtcaac aaccaccact tcaagtgcac ctccgaggga
      181 gagggaaagc catacgaggg aacccaaacc ggacgtatca aggtcgtcga gggaggacca
      241 ctcccattcg ccttcgacat cctcgccacc tgcttcatgt acggatccaa gaccttcatc
      301 aaccacaccc aaggaatccc agacttcttc aagcaatcct tcccagaggg attcacctgg
      361 gagcgtgtca ccacctacga ggacggagga gtcctcaccg ccacccaaga cacctccctc
      421 caagacggat gcctcatcta caacgtcaag atccgtggag tcaacttccc atccaacgga
      481 ccagtcatgc aaaagaagac cctcggatgg gaggcctcca ccgagaccct ctacccagcc
      541 gacggaggac tcgagggacg ttgcgacatg gccctcaagc tcgtcggagg aggacacctc
      601 atctgcaacc tcaagaccac ctaccgttcc aagaagccag ccaagaacct caagatgcca
      661 ggagtctact tcgtcgaccg tcgtctcgag cgtatcaagg aggccgacaa cgagacctac
      721 gtcgagcaac acgaggtcgc cgtcgcccgt tactgcgacc tcccatccaa gctcggacac
      781 aagctcaacg gaatggacga gctctacaag gaacaaaaac tgatatcaga agaggatctg
      841 gatcctctag agtcg
//
