LOCUS       sequence-182244-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of secretory immunoglobulin heavy chains in mammalian 
            cells, human IgG3 isotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  TITLE     Immunoglobulin expression vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AbVec2.0-IGHG3
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        629..832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     783..803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     829..853
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        1063..1081
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1063..1080
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     1094..1113
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2417..2426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2430..2564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2467..2486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2521..2540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        2633..2990
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2633..2824
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2656..2676)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2841..2976
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3010..3027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3010..3026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3019..3041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3239..3694
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3326..3345)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3536..3557
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3711..3730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3830..3852
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3890..3908)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3976..4080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4081..4149,4150..4941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4299..4318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5112..5700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5601..5620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5854..5871
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga
      241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
     1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
     1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
     1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa
     1201 ctgcaaccgg ttgactaact agctggtcga ccaagggccc aagcgtcttc cccctggcgc
     1261 cctgctccag gagcacctct gggggcacag cggccctggg ctgcctggtc aaggactact
     1321 tcccagagcc ggtgacggtg tcgtggaact caggcgccct gaccagcggc gtgcacacct
     1381 tcccggctgt cctacagtcc tcaggactct actccctcag cagcgtggtg accgtgccct
     1441 ccagcagctt gggcacccag acctacacct gcaacgtgaa tcacaagccc agcaacacca
     1501 aggtggacaa gagagttgag ctgaaaacac cactgggcga tacaacacac acttgcccaa
     1561 gatgccctga accaaaatct tgcgacacac ctccgccttg tccacgatgt cctgagccaa
     1621 agagttgcga tacccctcct ccttgtcccc ggtgtcccga acccaagtcc tgtgatacgc
     1681 ctccaccatg tcccagatgt ccagcacctg aactcctggg aggaccgtca gtcttcctct
     1741 tccccccaaa acccaaggat acccttatga tttcccggac ccctgaggtc acgtgcgtgg
     1801 tggtggacgt gagccacgaa gaccccgagg tccagttcaa gtggtacgtg gacggcgtgg
     1861 aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg ttccgtgtgg
     1921 tcagcgtcct caccgtcctg caccaggact ggctgaacgg caaggagtac aagtgcaagg
     1981 tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaaacc aaaggacagc
     2041 cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg
     2101 tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg gagtgggaga
     2161 gcagcgggca gccggagaac aactacaaca ccacgcctcc catgctggac tccgacggct
     2221 ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacatct
     2281 tctcatgctc cgtgatgcat gaggctctgc acaaccgctt cacgcagaag agcctctccc
     2341 tgtctccggg taaatgagtg cgacggccgg caagcccccg ctccccgggc tctcggggtc
     2401 gcgcgaggaa agcttggccg ccatggccca acttgtttat tgcagcttat aatggttaca
     2461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgggaat taattcggcg
     2581 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg
     2641 gaaagaacca tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     2701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     2761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2821 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2881 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     2941 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctgttaa
     3001 cagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     3061 acttaatcgc cttgcagcac atcccccttt cgccagttgg cgtaatagcg aagaggcccg
     3121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     3181 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     3241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3421 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     3481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3541 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
     3601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3661 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     3721 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     3781 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     3841 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     3901 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4141 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4201 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4261 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4321 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4381 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4441 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4501 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4561 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4621 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4681 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4741 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4801 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4861 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4921 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5821 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5881 ga
//
