LOCUS       sequence-18225-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  TITLE     aP2 promoter
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBS aP2 promoter (5.4kb) polyA
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    88..209
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(99..118)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     153..172
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(258..277)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(259..277)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     287..303
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     287..303
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 aaagggaaca aaagctggag ctccaccgcg gtggagctcg agtcagtgag cgaggaagcg
       61 gaagagtcta gagtcgacca gacatgataa gatacattga tgagtttgga caaaccacaa
      121 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      181 taaccattat aagctgcaat aaacaagttc tgctttaata agatctgatt cgaattccaa
      241 gcttggatcc gaattcgccc tatagtgagt cgtattacgc ggccgctcta gaactagtgg
      301 atcccccggg ctgcagcaca ggagggtgct atgagcctct gaagtccaga tagctcactt
      361 ttaaagatgc cctgaccatg tgactgtagg agtgaccaat gggggccaga tcatttcctt
      421 catgaccaga ccctgtatgt tttcctctga gtcatgtttt taatagaaat ttctcaactt
      481 tggttctccc tggcaatgat cactggactt agagtacaaa ttatttttaa ccatgaacag
      541 agtattttaa aggttcctgt tttgactgtc aaaagctaat gcattgaact tccccccatt
      601 attccttatg gatttgcctc attgtggagg agacaattat cttggacaca tttgaccttc
      661 ttatcttgag tttttatttt attaatactg caataatgtg tttagttctt ctgaatttga
      721 gaacataaaa actatcttag agattcttag tcttaatggc tcttttgtta gaatagtgtt
      781 tatctca
//
