LOCUS       sequence-182256-       13320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant 
            Selection: 2x35S:hpt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_21J
FEATURES             Location/Qualifiers
     source          1..13320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(2876..3552)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        4120..4465
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4587..4652
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4659..4679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5118..5423)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5220..5239)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6497..6553
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6557..6622
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6629..6649
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6977..7384)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     7234..7256
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7248..7265
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7249..7265
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7275..7294
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7275..7293
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7341..7361)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7341..7359)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7380..7396)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7380..7396)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7393..7415)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7404..7420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7428..7434,7435..7452,7453..7458))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7473..7494
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7680..8267
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      8281..8529
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8550..8574
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9875..10504
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10933..12006
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12072..12266
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12502..12524)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12610..12750
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12665..12684
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12765..12782)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12936..13320,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13016..13035)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaaatag agcttgtcga
     1801 tcgttaatta atcacctcca agatcccgga cgagtgctgg ggcgtcggtt tccactatcg
     1861 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     1921 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     1981 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     2041 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     2101 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     2161 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     2221 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     2281 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     2341 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     2401 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     2461 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     2521 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     2581 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     2641 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     2701 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     2761 atcaggtcgg acacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     2821 agttcaggct ttttcatcct aggagagata gatttgtaga gagagactgg tgatttcagc
     2881 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     2941 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     3001 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     3061 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     3121 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     3181 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     3241 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     3301 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     3361 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     3421 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     3481 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     3541 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     3601 acgagagtgt cgtgctccac catgggcgcg ccagatttgc cttttcaatt tcagaaagaa
     3661 tgctaaccca cagatggtta gagaggctta cgcagcaggt atcatcaaga cgatctaccc
     3721 gagcaataat ctccaggaaa tcaaatacct tcccaagaag gttaaagatg cagtcaaaag
     3781 attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga tcagaagtac
     3841 tattccagta tggacgattc aaggcttgct tcacaaacca aggcaagtaa tagagattgg
     3901 agtctctaaa aaggtagttc ccactgaatc aaaggccatg gagtcaaaga ttcaaataga
     3961 ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact
     4021 caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa
     4081 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
     4141 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
     4201 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     4261 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     4321 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     4381 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     4441 aagttcattt catttggaga gaacacgggg gactcctgca gggaattcca agcaacgaac
     4501 tgcgagtgat tcaagaaaaa agaaaacctg agctttcgat ctcttcggag tggtttcttg
     4561 ttctttgaaa aagaggggga agcatggatt ataaggatca cgatggcgac tacaaggacc
     4621 acgatattga ctacaaagac gatgacgata aaatggctcc taagaaaaag cgcaaagtcg
     4681 gtatccatgg cgttccctct agaatggtgg atctacgcac gctcggctac agtcagcagc
     4741 agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg
     4801 tgggccatgg gtttacacac gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag
     4861 ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt gccagaggcg acacacgaag
     4921 acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg
     4981 atgcggggga gttgagaggt ccgccgttac agttggacac aggccaactt gtgaagattg
     5041 caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc cagcaggaat gcgttaacag
     5101 gtgcccccct ggagacgtta tattccccag aacatcaggt taatggcgtt tttgatgtca
     5161 ttttcgcggt ggctgagatc agccacttct tccccgataa cggaaaccgg cacactggcc
     5221 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
     5281 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
     5341 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
     5401 taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt
     5461 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
     5521 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
     5581 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
     5641 ttcatagcat acctcctttt gacatacttc gggtatacat atcagtatat attcttatac
     5701 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttcgtctcca acgaccacct
     5761 cgtcgccttg gcctgcctcg gcggacgtcc tgccatggat gcagtgaaaa agggattgcc
     5821 gcacgcgccg gaattgatca gaagagtcaa tcgccgtatt ggcgaacgca cgtcccatcg
     5881 cgttgccgga tcccagctgg tgaagtccga gctggaagaa aaaaagagcg agctgcgcca
     5941 caagctcaag tacgtgcccc acgagtacat cgagctgatc gagatcgccc gcaacagcac
     6001 ccaagaccgc atcctggaga tgaaagtgat ggagttcttc atgaaggtgt acggctaccg
     6061 cggcaagcac ctgggcggct cccgcaagcc cgatggcgcc atctacaccg tgggctcccc
     6121 catcgactat ggcgtcattg tcgacaccaa ggcctactcc ggcggctaca acttacccat
     6181 cggtcaggcc gacgagatgc aacgctacgt gaaggagaac cagacccgca ataagcacat
     6241 taatcccaac gagtggtgga aggtgtaccc ctcctccgtg accgagttca aattcctgtt
     6301 cgtgtccggc cacttcaagg gcaattataa ggcccaactg acccgcctga accgcaagac
     6361 caactgcaac ggcgccgtgc tgtccgtgga ggaactgctg atcggcggcg agatgatcaa
     6421 ggctggtacc ctgaccctgg aagaggtgcg ccgcaagttc aacaatggtg aaatcaattt
     6481 caggtccgga agcggcgcca cgaacttctc tctgttaaag caagcaggtg acgtggaaga
     6541 aaacccaggg cccatggact acaaagacca tgacggtgat tataaagatc atgacatcga
     6601 ttacaaggat gacgatgaca agatggcccc caagaagaag aggaaggtgg gcattcacgg
     6661 ggtgccggct agcatggtgg atctacgcac gctcggctac agtcagcagc agcaagagaa
     6721 gatcaaaccg aaggtgcgtt cgacagtggc gcagcaccac gaggcactgg tgggccatgg
     6781 gtttacacac gcgcacatcg ttgcgctcag ccaacacccg gcagcgttag ggaccgtcgc
     6841 tgtcacgtat cagcacataa tcacggcgtt gccagaggcg acacacgaag acatcgttgg
     6901 cgtcggcaaa cagtggtccg gcgcacgcgc cctggaggcc ttgctcacgg atgcggggga
     6961 gttgagaggt ccgccgttac agttggacac aggccaactt gtgaagattg caaaacgtgg
     7021 cggcgtgacc gcaatggagg cagtgcatgc atcgcgcaat gcactgacgg gtgcccccct
     7081 ggagaccggc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     7141 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     7201 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     7261 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccggg ccgccccttg
     7321 aggtcctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     7381 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7441 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7501 ttgcgctcac tgcccgcttt ccaccggtgg tctccaacga tcatctggtg gcactcgcgt
     7561 gcctcggcgg acgtcctgcc atggatgcag tgaaaaaggg attgccgcac gcgccggaat
     7621 tgatcagaag agtcaatcgc cgtattggcg aacgcacgtc ccatcgcgtt gccagatctc
     7681 aactagtcaa aagtgaactg gaggagaaga aatctgaact tcgtcataaa ttgaaatatg
     7741 tgcctcatga atatattgaa ttaattgaaa ttgccagaaa ttccactcag gatagaattc
     7801 ttgaaatgaa ggtaatggaa ttttttatga aagtttatgg atatagaggt aaacatttgg
     7861 gtggatcaag gaaaccggac ggagcaattt atactgtcgg atctcctatt gattacggtg
     7921 tgatcgtgga tactaaagct tatagcggag gttataatct gccaattggc caagcagatg
     7981 aaatggagcg atatgtcgaa gaaaatcaaa cacgagacaa acatctcaac cctaatgaat
     8041 ggtggaaagt ctatccatct tctgtaacgg aatttaagtt tttatttgtg agtggtcact
     8101 ttaaaggaaa ctacaaagct cagcttacac gattaaatca tatcactaat tgtaatggag
     8161 ctgttcttag tgtagaagag cttttaattg gtggagaaat gattaaagcc ggcacattaa
     8221 ccttagagga agtgaggcgg aaatttaata acggcgagat aaacttttaa tagctcgaga
     8281 tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg
     8341 tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg
     8401 taagatcaca ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg
     8461 gatctcttct gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt
     8521 aaagataagg agctcaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     8581 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     8641 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     8701 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     8761 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     8821 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     8881 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     8941 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     9001 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     9061 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     9121 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     9181 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     9241 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     9301 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     9361 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     9421 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     9481 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     9541 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     9601 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     9661 agccgcccgc gcacggctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     9721 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     9781 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     9841 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     9901 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     9961 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
    10021 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
    10081 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
    10141 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
    10201 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
    10261 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
    10321 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
    10381 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
    10441 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
    10501 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
    10561 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
    10621 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
    10681 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
    10741 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
    10801 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
    10861 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
    10921 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
    10981 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
    11041 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
    11101 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
    11161 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
    11221 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
    11281 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
    11341 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
    11401 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
    11461 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
    11521 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
    11581 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
    11641 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
    11701 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
    11761 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
    11821 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
    11881 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
    11941 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
    12001 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
    12061 ggcctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
    12121 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
    12181 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
    12241 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
    12301 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
    12361 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
    12421 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
    12481 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggaaacgg tcacagcttg
    12541 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
    12601 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
    12661 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
    12721 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
    12781 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    12841 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    12901 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    12961 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    13021 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    13081 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    13141 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    13201 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    13261 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
//
