LOCUS       sequence-182263-       13092 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant 
            Selection: 2x35S:npt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13092)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13092)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_22J
FEATURES             Location/Qualifiers
     source          1..13092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1812..2609)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1923..1942)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2533..2552
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2648..3324)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        3892..4237
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4359..4424
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4431..4451
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4890..5195)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4992..5011)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6269..6325
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6329..6394
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6401..6421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6749..7156)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     7006..7028
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7020..7037
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7021..7037
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7047..7066
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7047..7065
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7113..7133)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7113..7131)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7152..7168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7152..7168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7165..7187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7176..7192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7200..7206,7207..7224,7225..7230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7245..7266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7452..8039
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      8053..8301
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8322..8346
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9647..10276
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10705..11778
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11844..12038
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12274..12296)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12382..12522
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12437..12456
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12537..12554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12708..13092,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12788..12807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaaatag agcttgtcga
     1801 tcgttaatta atcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     1861 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     1921 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     1981 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     2041 ccatgtgtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     2101 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     2161 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     2221 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     2281 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     2341 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     2401 cacgatagcc gcgctgcctc gtcctggagt tcattcaggg caccggacag gtcggtcttg
     2461 acaaaaagaa ccgggcgccc ctgcgctgac agccgaaaca cggcggcatc agagcagccg
     2521 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     2581 gcgtgcaatc catcttgttc aatccccatc ctaggagaga tagatttgta gagagagact
     2641 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
     2701 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
     2761 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
     2821 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
     2881 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
     2941 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
     3001 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
     3061 gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     3121 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     3181 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
     3241 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     3301 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     3361 ttcttggagt agacgagagt gtcgtgctcc accatgggcg cgccagattt gccttttcaa
     3421 tttcagaaag aatgctaacc cacagatggt tagagaggct tacgcagcag gtatcatcaa
     3481 gacgatctac ccgagcaata atctccagga aatcaaatac cttcccaaga aggttaaaga
     3541 tgcagtcaaa agattcagga ctaactgcat caagaacaca gagaaagata tatttctcaa
     3601 gatcagaagt actattccag tatggacgat tcaaggcttg cttcacaaac caaggcaagt
     3661 aatagagatt ggagtctcta aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa
     3721 gattcaaata gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag
     3781 tctcttacga ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact
     3841 tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt
     3901 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt
     3961 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
     4021 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac
     4081 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg
     4141 tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
     4201 ctctatataa ggaagttcat ttcatttgga gagaacacgg gggactcctg cagggaattc
     4261 caagcaacga actgcgagtg attcaagaaa aaagaaaacc tgagctttcg atctcttcgg
     4321 agtggtttct tgttctttga aaaagagggg gaagcatgga ttataaggat cacgatggcg
     4381 actacaagga ccacgatatt gactacaaag acgatgacga taaaatggct cctaagaaaa
     4441 agcgcaaagt cggtatccat ggcgttccct ctagaatggt ggatctacgc acgctcggct
     4501 acagtcagca gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc
     4561 acgaggcact ggtgggccat gggtttacac acgcgcacat cgttgcgctc agccaacacc
     4621 cggcagcgtt agggaccgtc gctgtcacgt atcagcacat aatcacggcg ttgccagagg
     4681 cgacacacga agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc gccctggagg
     4741 ccttgctcac ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac
     4801 ttgtgaagat tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gccagcagga
     4861 atgcgttaac aggtgccccc ctggagacgt tatattcccc agaacatcag gttaatggcg
     4921 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggaaacc
     4981 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
     5041 gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc
     5101 atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
     5161 ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc
     5221 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
     5281 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc
     5341 ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
     5401 gtaatacgct gcttcatagc atacctcctt ttgacatact tcgggtatac atatcagtat
     5461 atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttcgtctc
     5521 caacgaccac ctcgtcgcct tggcctgcct cggcggacgt cctgccatgg atgcagtgaa
     5581 aaagggattg ccgcacgcgc cggaattgat cagaagagtc aatcgccgta ttggcgaacg
     5641 cacgtcccat cgcgttgccg gatcccagct ggtgaagtcc gagctggaag aaaaaaagag
     5701 cgagctgcgc cacaagctca agtacgtgcc ccacgagtac atcgagctga tcgagatcgc
     5761 ccgcaacagc acccaagacc gcatcctgga gatgaaagtg atggagttct tcatgaaggt
     5821 gtacggctac cgcggcaagc acctgggcgg ctcccgcaag cccgatggcg ccatctacac
     5881 cgtgggctcc cccatcgact atggcgtcat tgtcgacacc aaggcctact ccggcggcta
     5941 caacttaccc atcggtcagg ccgacgagat gcaacgctac gtgaaggaga accagacccg
     6001 caataagcac attaatccca acgagtggtg gaaggtgtac ccctcctccg tgaccgagtt
     6061 caaattcctg ttcgtgtccg gccacttcaa gggcaattat aaggcccaac tgacccgcct
     6121 gaaccgcaag accaactgca acggcgccgt gctgtccgtg gaggaactgc tgatcggcgg
     6181 cgagatgatc aaggctggta ccctgaccct ggaagaggtg cgccgcaagt tcaacaatgg
     6241 tgaaatcaat ttcaggtccg gaagcggcgc cacgaacttc tctctgttaa agcaagcagg
     6301 tgacgtggaa gaaaacccag ggcccatgga ctacaaagac catgacggtg attataaaga
     6361 tcatgacatc gattacaagg atgacgatga caagatggcc cccaagaaga agaggaaggt
     6421 gggcattcac ggggtgccgg ctagcatggt ggatctacgc acgctcggct acagtcagca
     6481 gcagcaagag aagatcaaac cgaaggtgcg ttcgacagtg gcgcagcacc acgaggcact
     6541 ggtgggccat gggtttacac acgcgcacat cgttgcgctc agccaacacc cggcagcgtt
     6601 agggaccgtc gctgtcacgt atcagcacat aatcacggcg ttgccagagg cgacacacga
     6661 agacatcgtt ggcgtcggca aacagtggtc cggcgcacgc gccctggagg ccttgctcac
     6721 ggatgcgggg gagttgagag gtccgccgtt acagttggac acaggccaac ttgtgaagat
     6781 tgcaaaacgt ggcggcgtga ccgcaatgga ggcagtgcat gcatcgcgca atgcactgac
     6841 gggtgccccc ctggagaccg gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     6901 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6961 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     7021 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtaccg
     7081 ggccgcccct tgaggtcctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     7141 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7201 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7261 acattaattg cgttgcgctc actgcccgct ttccaccggt ggtctccaac gatcatctgg
     7321 tggcactcgc gtgcctcggc ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc
     7381 acgcgccgga attgatcaga agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg
     7441 ttgccagatc tcaactagtc aaaagtgaac tggaggagaa gaaatctgaa cttcgtcata
     7501 aattgaaata tgtgcctcat gaatatattg aattaattga aattgccaga aattccactc
     7561 aggatagaat tcttgaaatg aaggtaatgg aattttttat gaaagtttat ggatatagag
     7621 gtaaacattt gggtggatca aggaaaccgg acggagcaat ttatactgtc ggatctccta
     7681 ttgattacgg tgtgatcgtg gatactaaag cttatagcgg aggttataat ctgccaattg
     7741 gccaagcaga tgaaatggag cgatatgtcg aagaaaatca aacacgagac aaacatctca
     7801 accctaatga atggtggaaa gtctatccat cttctgtaac ggaatttaag tttttatttg
     7861 tgagtggtca ctttaaagga aactacaaag ctcagcttac acgattaaat catatcacta
     7921 attgtaatgg agctgttctt agtgtagaag agcttttaat tggtggagaa atgattaaag
     7981 ccggcacatt aaccttagag gaagtgaggc ggaaatttaa taacggcgag ataaactttt
     8041 aatagctcga gatatgaaga tgaagatgaa atatttggtg tgtcaaataa aaagcttgtg
     8101 tgcttaagtt tgtgtttttt tcttggcttg ttgtgttatg aatttgtggc tttttctaat
     8161 attaaatgaa tgtaagatca cattataatg aataaacaaa tgtttctata atccattgtg
     8221 aatgttttgt tggatctctt ctgcagcata taactactgt atgtgctatg gtatggacta
     8281 tggaatatga ttaaagataa ggagctcaaa ctatcagtgt ttgacaggat atattggcgg
     8341 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     8401 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     8461 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     8521 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     8581 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     8641 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     8701 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     8761 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     8821 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     8881 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     8941 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     9001 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     9061 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     9121 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     9181 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     9241 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     9301 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     9361 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     9421 ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa atcctggccg
     9481 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     9541 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     9601 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     9661 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     9721 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     9781 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     9841 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     9901 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     9961 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
    10021 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
    10081 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
    10141 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
    10201 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
    10261 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
    10321 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
    10381 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
    10441 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
    10501 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
    10561 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
    10621 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
    10681 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
    10741 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
    10801 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
    10861 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
    10921 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
    10981 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
    11041 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
    11101 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
    11161 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
    11221 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
    11281 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
    11341 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
    11401 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
    11461 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
    11521 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
    11581 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
    11641 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
    11701 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
    11761 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
    11821 aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
    11881 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
    11941 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
    12001 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
    12061 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
    12121 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
    12181 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
    12241 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac
    12301 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    12361 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
    12421 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    12481 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
    12541 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    12601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    12661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    12721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    12901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    13021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    13081 agagcgaggt at
//
