LOCUS       sequence-182270-       12849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant 
            Selection: 2x35S:bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12849)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_23J
FEATURES             Location/Qualifiers
     source          1..12849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1815..2366)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2405..3081)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        3649..3994
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4116..4181
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4188..4208
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4647..4952)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4749..4768)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6026..6082
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6086..6151
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6158..6178
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6506..6913)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     6763..6785
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6777..6794
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6778..6794
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6804..6823
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6804..6822
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6870..6890)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6870..6888)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6909..6925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6909..6925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6922..6944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6933..6949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6957..6963,6964..6981,6982..6987))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7002..7023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7209..7796
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      7810..8058
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8079..8103
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9404..10033
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10462..11535
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11601..11795
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12031..12053)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12139..12279
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12194..12213
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12294..12311)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12465..12849,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12545..12564)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaaatag agcttgtcga
     1801 tcgttaatta atcatcagat ttcggtgacg ggcaggaccg gacggggcgg caccggcagg
     1861 ctgaagtcca gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc
     1921 cgcagcatgc cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg
     1981 ggcagcccga tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg
     2041 tgggtgtaga gcgtggagcc cagtcccgtc cgctggtggc gggggctgac gtacacggtg
     2101 gactcggccg tccagtcgta ggcgttgcgt gccttccagg gacccgcgta ggcgatgccg
     2161 gcgacctcgc cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg
     2221 tcgtccgtcc actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg
     2281 atgtagtggt tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg
     2341 tcggccgggc gtcgttctgg gctcatccta ggagagatag atttgtagag agagactggt
     2401 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg
     2461 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
     2521 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     2581 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     2641 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     2701 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     2761 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     2821 ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     2881 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     2941 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     3001 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     3061 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     3121 ttggagtaga cgagagtgtc gtgctccacc atgggcgcgc cagatttgcc ttttcaattt
     3181 cagaaagaat gctaacccac agatggttag agaggcttac gcagcaggta tcatcaagac
     3241 gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc
     3301 agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat
     3361 cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat
     3421 agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat
     3481 tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct
     3541 cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt
     3601 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
     3661 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
     3721 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
     3781 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     3841 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     3901 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
     3961 tatataagga agttcatttc atttggagag aacacggggg actcctgcag ggaattccaa
     4021 gcaacgaact gcgagtgatt caagaaaaaa gaaaacctga gctttcgatc tcttcggagt
     4081 ggtttcttgt tctttgaaaa agagggggaa gcatggatta taaggatcac gatggcgact
     4141 acaaggacca cgatattgac tacaaagacg atgacgataa aatggctcct aagaaaaagc
     4201 gcaaagtcgg tatccatggc gttccctcta gaatggtgga tctacgcacg ctcggctaca
     4261 gtcagcagca gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg
     4321 aggcactggt gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg
     4381 cagcgttagg gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga
     4441 cacacgaaga catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct
     4501 tgctcacgga tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg
     4561 tgaagattgc aaaacgtggc ggcgtgaccg caatggaggc agtgcatgcc agcaggaatg
     4621 cgttaacagg tgcccccctg gagacgttat attccccaga acatcaggtt aatggcgttt
     4681 ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac ggaaaccggc
     4741 acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg
     4801 taaagttcac gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc
     4861 cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc
     4921 tctcttttat aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa
     4981 agagccgttc atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt
     5041 ccagcgttcg gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga
     5101 gatattgaca tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta
     5161 atacgctgct tcatagcata cctccttttg acatacttcg ggtatacata tcagtatata
     5221 ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tcgtctccaa
     5281 cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct gccatggatg cagtgaaaaa
     5341 gggattgccg cacgcgccgg aattgatcag aagagtcaat cgccgtattg gcgaacgcac
     5401 gtcccatcgc gttgccggat cccagctggt gaagtccgag ctggaagaaa aaaagagcga
     5461 gctgcgccac aagctcaagt acgtgcccca cgagtacatc gagctgatcg agatcgcccg
     5521 caacagcacc caagaccgca tcctggagat gaaagtgatg gagttcttca tgaaggtgta
     5581 cggctaccgc ggcaagcacc tgggcggctc ccgcaagccc gatggcgcca tctacaccgt
     5641 gggctccccc atcgactatg gcgtcattgt cgacaccaag gcctactccg gcggctacaa
     5701 cttacccatc ggtcaggccg acgagatgca acgctacgtg aaggagaacc agacccgcaa
     5761 taagcacatt aatcccaacg agtggtggaa ggtgtacccc tcctccgtga ccgagttcaa
     5821 attcctgttc gtgtccggcc acttcaaggg caattataag gcccaactga cccgcctgaa
     5881 ccgcaagacc aactgcaacg gcgccgtgct gtccgtggag gaactgctga tcggcggcga
     5941 gatgatcaag gctggtaccc tgaccctgga agaggtgcgc cgcaagttca acaatggtga
     6001 aatcaatttc aggtccggaa gcggcgccac gaacttctct ctgttaaagc aagcaggtga
     6061 cgtggaagaa aacccagggc ccatggacta caaagaccat gacggtgatt ataaagatca
     6121 tgacatcgat tacaaggatg acgatgacaa gatggccccc aagaagaaga ggaaggtggg
     6181 cattcacggg gtgccggcta gcatggtgga tctacgcacg ctcggctaca gtcagcagca
     6241 gcaagagaag atcaaaccga aggtgcgttc gacagtggcg cagcaccacg aggcactggt
     6301 gggccatggg tttacacacg cgcacatcgt tgcgctcagc caacacccgg cagcgttagg
     6361 gaccgtcgct gtcacgtatc agcacataat cacggcgttg ccagaggcga cacacgaaga
     6421 catcgttggc gtcggcaaac agtggtccgg cgcacgcgcc ctggaggcct tgctcacgga
     6481 tgcgggggag ttgagaggtc cgccgttaca gttggacaca ggccaacttg tgaagattgc
     6541 aaaacgtggc ggcgtgaccg caatggaggc agtgcatgca tcgcgcaatg cactgacggg
     6601 tgcccccctg gagaccggcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     6661 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6721 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6781 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg ggtaccgggc
     6841 cgccccttga ggtcctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     6901 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6961 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7021 ttaattgcgt tgcgctcact gcccgctttc caccggtggt ctccaacgat catctggtgg
     7081 cactcgcgtg cctcggcgga cgtcctgcca tggatgcagt gaaaaaggga ttgccgcacg
     7141 cgccggaatt gatcagaaga gtcaatcgcc gtattggcga acgcacgtcc catcgcgttg
     7201 ccagatctca actagtcaaa agtgaactgg aggagaagaa atctgaactt cgtcataaat
     7261 tgaaatatgt gcctcatgaa tatattgaat taattgaaat tgccagaaat tccactcagg
     7321 atagaattct tgaaatgaag gtaatggaat tttttatgaa agtttatgga tatagaggta
     7381 aacatttggg tggatcaagg aaaccggacg gagcaattta tactgtcgga tctcctattg
     7441 attacggtgt gatcgtggat actaaagctt atagcggagg ttataatctg ccaattggcc
     7501 aagcagatga aatggagcga tatgtcgaag aaaatcaaac acgagacaaa catctcaacc
     7561 ctaatgaatg gtggaaagtc tatccatctt ctgtaacgga atttaagttt ttatttgtga
     7621 gtggtcactt taaaggaaac tacaaagctc agcttacacg attaaatcat atcactaatt
     7681 gtaatggagc tgttcttagt gtagaagagc ttttaattgg tggagaaatg attaaagccg
     7741 gcacattaac cttagaggaa gtgaggcgga aatttaataa cggcgagata aacttttaat
     7801 agctcgagat atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc
     7861 ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt
     7921 aaatgaatgt aagatcacat tataatgaat aaacaaatgt ttctataatc cattgtgaat
     7981 gttttgttgg atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg
     8041 aatatgatta aagataagga gctcaaacta tcagtgtttg acaggatata ttggcgggta
     8101 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     8161 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
     8221 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
     8281 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
     8341 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
     8401 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
     8461 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
     8521 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
     8581 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
     8641 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
     8701 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
     8761 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
     8821 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
     8881 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
     8941 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
     9001 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
     9061 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
     9121 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
     9181 acgtgttcga gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt
     9241 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
     9301 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     9361 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     9421 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     9481 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     9541 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     9601 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     9661 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     9721 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     9781 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     9841 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     9901 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     9961 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
    10021 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
    10081 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
    10141 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
    10201 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
    10261 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
    10321 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
    10381 gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg gcactggaac
    10441 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
    10501 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
    10561 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
    10621 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
    10681 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
    10741 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
    10801 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
    10861 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
    10921 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
    10981 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
    11041 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
    11101 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
    11161 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
    11221 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
    11281 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
    11341 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
    11401 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
    11461 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
    11521 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
    11581 ggtcgaaaag gcctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
    11641 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
    11701 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
    11761 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
    11821 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
    11881 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
    11941 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccctg
    12001 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggaaacggt
    12061 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    12121 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
    12181 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
    12241 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
    12301 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
    12361 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
    12421 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
    12481 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
    12541 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
    12601 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
    12661 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
    12721 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
    12781 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
    12841 gcgaggtat
//
