LOCUS       sequence-182274-        7208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cilia-membrane-targeted inositol polyphosphate 5-phosphatase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Phua SC, Chiba S, Suzuki M, Su E, Roberson EC, Pusapati GV, Setou M,
            Rohatgi R, Reiter JF, Ikegami K, Inoue T
  TITLE     Dynamic Remodeling of Membrane Composition Drives Cell Cycle through
            Primary Cilia Excision.
  JOURNAL   Cell. 2017 Jan 12;168(1-2):264-279.e15. doi: 
            10.1016/j.cell.2016.12.032. Epub 2017 Jan 12.
  PUBMED    28086093
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2017-01-12"; volume: "168(1-2):264-279.e15. doi"; pages: " 
            10.1016/j.cell.2016.12.032. Epub 2017 Jan 12"
COMMENT     Sequence Label: 5HT6-YFP-Inp54p
FEATURES             Location/Qualifiers
     source          1..7208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        91..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        395..598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     549..569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      627..636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1983..1992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1989..1991,1992..1994,1995..2705)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2034..2055)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2295..2314)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2642..2663
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4026..4147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4063..4082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4117..4136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4154..4609)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4291..4312)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4503..4522
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4636..4740
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4742..5099
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4742..4933
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4765..4785)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4950..5085
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5134..5928
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5134..5928
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5188..5207)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5798..5817
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6116..6135)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6160..6207
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6536..7124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7025..7044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      421 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      481 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      601 tttagtgaac cgtcagatcc gctagcgcca ccatggttcc agagcccggc cctgtcaaca
      661 gtagcacccc agcctggggc cccgggccac cgccagcccc agggggcagc ggctgggtgg
      721 ccgctgcgct gtgcgtggtc atcgtgctga cggcagccgc caattcgctg ctgatcgcgc
      781 tcatctgcac gcagcccgcg ctgcgcaaca cgtctaactt cttcctggtg tcgctcttca
      841 cgtcggacct gatggtggga ttggtggtga tgcccccagc catgctgaac gcgctgtatg
      901 ggcgctgggt actagctcgc ggcctctgtc tgctttggac cgccttcgac gtgatgtgct
      961 gcagcgcctc cattctcaac ctctgcctca tcagcctgga ccgctacctg ctcatcctct
     1021 cgccgctgcg ctacaagctg cgcatgacag ccccgcgtgc cctggcgctc atcctgggtg
     1081 cctggagcct cgccgcgctg gcctccttcc tgcccctctt gctgggctgg catgaactgg
     1141 gcaaagctcg aacatctgcc ccgggccagt gccgcctatt ggccagcctg ccttatgtcc
     1201 tcgtggcgtc cggcgtcacc tttttcctgc cttcgggtgc catctgcttc acctactgca
     1261 ggatcttgct ggctgcccgc aagcaggcgg tgcaagtggc ctcgctcacc acgggcacgg
     1321 ctacggctgg ccaggccttg gaaaccttgc aggtgcccag gacaccacgc ccagggatgg
     1381 agtctgctga cagtaggcgt ctgaccacca agcatagcag gaaggccttg aaggccagcc
     1441 tgaccctcgg catcctgctg agcatgttct ttgtcacctg gctgcccttc tttgtggcca
     1501 gcatagctca ggccgtatgt gactgcatct ctccaggtct cttcgatgtc ctcacatggc
     1561 tgggatactg taatagcacc atgaacccta tcatctatcc cctcttcatg cgggacttca
     1621 agagggccct gggcaggttc gtgccgtgtg tccactgtcc cccggagcac cgggccagcc
     1681 ccgcctcccc ctccatgtgg acctctcaca gtggtgccag gccaggcctc agcctgcagc
     1741 aggtgctgcc cctgcctctg ccacccaact cagattcaga ctcagcttca gggggcacct
     1801 cgggcctgca gctcacagcc cagcttttgc tgcctggaga ggcgacccgg gaccccccgc
     1861 cacccaccag ggcccctact gtggtcaact tcttcgtcac agactctgtg gagcctgaga
     1921 tacggcagca tccacttggt tcccccatga acagtgctgg tggtagcgca ggaggaccgg
     1981 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     2041 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     2101 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     2161 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
     2221 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2281 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2341 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2401 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2461 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2521 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2581 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2641 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2701 acaagtccgg actcagatct cgagctcaag cttcgaattc gatgaacaaa acgaattgga
     2761 aggtatctgt aactacattc aattgtggta aggaatttcc cgttgaaaat tcgaaagcaa
     2821 tcgttaagca attgcttttc ccctacgatg acggcatctc ccaattggaa ttgcaagatc
     2881 tatatgtgtt ggggttccaa gaagtagtcc ccatatggca aggctccttc cctgctgtta
     2941 atcgtgattt aatcgataga attacaacta ctgcggttaa ttgtctgaat gaaaaagtgt
     3001 cagctacaca aggggatgag cagtattcat gtttaggggt gaatagtctt ggggcgatta
     3061 ccataatagt attatacaat aataatgcgc taaaggtaaa agatgatatt ttgaaacgga
     3121 atgggaaatg cggttggttt ggtacacact tgaaaggtgg aactttgata agttttcaga
     3181 tgacacgcaa tggcgaggag aactgggaaa gatttagcta tatttgtgct catttaaatg
     3241 caaatgaagg tgtcaacaat agaaatcaaa gaatagatga ttacaaacga ataatgagtg
     3301 aagtgtgtga ttctgaggtt gcaaaaagcg accacttttt ctttctaggc gatttgaatt
     3361 ttagggttac tagtacttat gatccaacta ccaattattc gtccaccaca acgttaaggc
     3421 gtctattaga aaatcatgaa gaactaaatc tgctacgtaa aggagaggat gaaccgttat
     3481 gcaaaggttt tcaggagctg aagataacat ttccaccgac atacaaattc aagttgtttg
     3541 aaaaggaaac atataataca aaaagaatcc cgtcgtggtg cgataggatt ctctacaaaa
     3601 gctatgcagt gccaactttc gcacaagaag gtacttacca ttccgtccct aggtccaatg
     3661 ctctattatt tagcgatcat caacccgtta atcttaccgt aagattacca aggtctacag
     3721 ggacgccagt gccgtgatcc ttacatatcg aaaagtatcc tttatcatgg agctctggcc
     3781 tgattggaca aattggtgac gcagtcatcg ggtactgcgg ttggctagtt actaagaatg
     3841 tacattattg gatattaggt tctttactat tatatttact attaaaaatc ctgtaaggat
     3901 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gttttacttg
     3961 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4021 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4141 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     4201 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     4261 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     4321 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     4381 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     4441 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4501 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     4561 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4621 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4681 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4741 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     4801 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     4861 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4921 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     4981 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5041 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag
     5101 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5161 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5221 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5281 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     5341 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5401 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5461 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5521 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5581 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5641 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     5701 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5761 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5821 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5881 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5941 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6001 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6061 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa
     6121 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata
     6181 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     6241 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     6301 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     6361 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt
     6421 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6481 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6541 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6601 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6661 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6721 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6781 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6841 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6901 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6961 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7021 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7081 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7141 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7201 cccctgat
//
