LOCUS       sequence-182281-       19955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 
            ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant 
            Selection: PvUbi2:hpt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19955)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 19955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_25H
FEATURES             Location/Qualifiers
     source          1..19955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4828..4833,4834..4851,4852..4858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4866..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4871..4893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4890..4906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4890..4906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             7523..7579
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7580..11683
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7580..11683
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11684..11731
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      11743..11991
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(12727..12738)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    14005..14024
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        14025..14100
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    14101..14120
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     CDS             14440..14745
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     14624..14643
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     polyA_signal    14773..14947
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(14966..14983)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14966..14982)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14975..14997)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    15185..15209
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             16510..17139
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             17568..18641
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      18707..18901
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(19137..19159)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    19245..19385
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     19300..19319
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(19400..19417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(19571..19955,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19651..19670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac
     2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat
     2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa
     3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa
     3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg
     3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc
     3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta
     3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat
     3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata
     3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca
     3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga
     3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt
     3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca
     3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc
     3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac
     3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca
     3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca
     3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg
     3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc
     3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat
     4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga
     4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt
     4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag
     4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg
     4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt
     4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat
     4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc
     4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt
     4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt
     4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta
     4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt
     4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag
     4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4921 gagctcggta cccggggatc tgcaggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc
     4981 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     5041 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     5101 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     5161 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     5221 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     5281 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     5341 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     5401 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     5461 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     5521 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     5581 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     5641 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     5701 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     5761 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     5821 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     5881 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     5941 cgctcgtcct cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
     6001 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
     6061 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
     6121 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
     6181 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
     6241 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
     6301 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt ctgtttcaaa
     6361 ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta
     6421 cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt
     6481 tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt
     6541 tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta
     6601 tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat
     6661 ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat
     6721 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata
     6781 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca
     6841 gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg
     6901 tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcaggaa gcatggacca
     6961 ctacctcgac atcaggctca ggccagaccc agagttccca ccagcccagc tcatgtccgt
     7021 cctcttcggc aagctccacc aggccctcgt ggcccagggc ggcgacagga tcggcgtgtc
     7081 cttcccagac ctcgacgagt ccaggtccag gctcggcgag aggctccgca tccacgcctc
     7141 cgccgacgac ctcagggccc tcctcgccag gccgtggctg gagggcctca gggaccacct
     7201 ccagttcggc gagccagccg tggtgccaca cccaacccca tacaggcaag tgtccagggt
     7261 gcaagccaag tccaacccag agaggctcag gaggaggctc atgaggaggc acgacctctc
     7321 cgaggaagag gccaggaagc gcatcccaga caccgtggcc agggccctcg acctcccatt
     7381 cgtgaccctc aggtcccagt ccaccggcca gcacttccgc ctcttcatca ggcacggccc
     7441 actccaggtg accgccgagg agggcggctt tacctgctac ggcctctcca agggcggctt
     7501 cgtgccgtgg ttcggctccg gcgccaccaa cttctccctc ctcaagcaag ccggcgacgt
     7561 ggaggagaac ccaggcccaa tggacaagaa gtactcgatc ggcctcgaca tcgggacgaa
     7621 ctcagttggc tgggccgtga tcaccgacga gtacaaggtg ccctctaaga agttcaaggt
     7681 cctggggaac accgaccgcc attccatcaa gaagaacctc atcggcgctc tcctgttcga
     7741 cagcggggag accgctgagg ctacgaggct caagagaacc gctaggcgcc ggtacacgag
     7801 aaggaagaac aggatctgct acctccaaga gattttctcc aacgagatgg ccaaggttga
     7861 cgattcattc ttccaccgcc tggaggagtc tttcctcgtg gaggaggata agaagcacga
     7921 gcggcatccc atcttcggca acatcgtgga cgaggttgcc taccacgaga agtaccctac
     7981 gatctaccat ctgcggaaga agctcgtgga ctccaccgat aaggcggacc tcagactgat
     8041 ctacctcgct ctggcccaca tgatcaagtt ccgcggccat ttcctgatcg agggggatct
     8101 caacccagac aacagcgatg ttgacaagct gttcatccaa ctcgtgcaga cctacaacca
     8161 actcttcgag gagaacccga tcaacgcctc tggcgtggac gcgaaggcta tcctgtccgc
     8221 gaggctctcg aagtccagga ggctggagaa cctgatcgct cagctcccag gcgagaagaa
     8281 gaacggcctg ttcgggaacc tcatcgctct cagcctgggg ctcaccccga acttcaagtc
     8341 gaacttcgat ctcgctgagg acgccaagct gcaactctcc aaggacacct acgacgatga
     8401 cctcgataac ctcctggccc agatcggcga tcaatacgcg gacctgttcc tcgctgccaa
     8461 gaacctgtcg gacgccatcc tcctgtcaga tatcctccgc gtgaacaccg agatcacgaa
     8521 ggctccactc tctgcctcca tgatcaagcg ctacgacgag caccatcagg atctgaccct
     8581 cctgaaggcg ctggtccgcc aacagctccc ggagaagtac aaggagattt tcttcgatca
     8641 gtcgaagaac ggctacgctg ggtacatcga cggcggggcc tcacaagagg agttctacaa
     8701 gttcatcaag ccaatcctgg agaagatgga cggcacggag gagctcctgg tgaagctcaa
     8761 cagggaggac ctcctgcgga agcagagaac cttcgataac ggcagcatcc cccaccaaat
     8821 ccatctcggg gagctgcacg ccatcctgag aaggcaagag gacttctacc ctttcctcaa
     8881 ggataaccgg gagaagatcg agaagatcct gaccttcaga atcccatact acgtcggccc
     8941 tctcgcgcgg gggaactcaa gattcgcttg gatgacccgc aagtctgagg agaccatcac
     9001 gccgtggaac ttcgaggagg tggtggacaa gggcgctagc gctcagtcgt tcatcgagag
     9061 gatgaccaac ttcgacaaga acctgcccaa cgagaaggtg ctccctaagc actcgctcct
     9121 gtacgagtac ttcaccgtct acaacgagct cacgaaggtg aagtacgtca ccgagggcat
     9181 gcgcaagcca gcgttcctgt ccggggagca gaagaaggct atcgtggacc tcctgttcaa
     9241 gaccaaccgg aaggtcacgg ttaagcaact caaggaggac tacttcaaga agatcgagtg
     9301 cttcgattcg gtcgagatca gcggcgttga ggaccgcttc aacgccagcc tcgggaccta
     9361 ccacgatctc ctgaagatca tcaaggataa ggacttcctg gacaacgagg agaacgagga
     9421 tatcctggag gacatcgtgc tgaccctcac gctgttcgag gacagggaga tgatcgagga
     9481 gcgcctgaag acgtacgccc atctcttcga tgacaaggtc atgaagcaac tcaagcgccg
     9541 gagatacacc ggctggggga ggctgtcccg caagctcatc aacggcatcc gggacaagca
     9601 gtccgggaag accatcctcg acttcctcaa gagcgatggc ttcgccaaca ggaacttcat
     9661 gcaactgatc cacgatgaca gcctcacctt caaggaggat atccaaaagg ctcaagtgag
     9721 cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctccc ccgcgatcaa
     9781 gaagggcatc ctccagaccg tgaaggttgt ggacgagctc gtgaaggtca tgggccggca
     9841 caagcctgag aacatcgtca tcgagatggc cagagagaac caaaccacgc agaaggggca
     9901 aaagaactct agggagcgca tgaagcgcat cgaggagggc atcaaggagc tggggtccca
     9961 aatcctcaag gagcacccag tggagaacac ccaactgcag aacgagaagc tctacctgta
    10021 ctacctccag aacggcaggg atatgtacgt ggaccaagag ctggatatca accgcctcag
    10081 cgattacgac gtcgatcata tcgttcccca gtctttcctg aaggatgact ccatcgacaa
    10141 caaggtcctc accaggtcgg acaagaaccg cggcaagtca gataacgttc catctgagga
    10201 ggtcgttaag aagatgaaga actactggag gcagctcctg aacgccaagc tgatcacgca
    10261 aaggaagttc gacaacctca ccaaggctga gagaggcggg ctctcagagc tggacaaggc
    10321 cggcttcatc aagcggcagc tggtcgagac cagacaaatc acgaagcacg ttgcgcaaat
    10381 cctcgactct cggatgaaca cgaagtacga tgagaacgac aagctgatca gggaggttaa
    10441 ggtgatcacc ctgaagtcta agctcgtctc cgacttcagg aaggatttcc agttctacaa
    10501 ggttcgcgag atcaacaact accaccatgc ccatgacgct tacctcaacg ctgtggtcgg
    10561 caccgctctg atcaagaagt acccaaagct ggagtccgag ttcgtgtacg gggactacaa
    10621 ggtttacgat gtgcgcaaga tgatcgccaa gtcggagcaa gagatcggca aggctaccgc
    10681 caagtacttc ttctactcaa acatcatgaa cttcttcaag accgagatca cgctggccaa
    10741 cggcgagatc cggaagagac cgctcatcga gaccaacggc gagacggggg agatcgtgtg
    10801 ggacaagggc agggatttcg cgaccgtccg caaggttctc tccatgcccc aggtgaacat
    10861 cgtcaagaag accgaggtcc aaacgggcgg gttctcaaag gagtctatcc tgcctaagcg
    10921 gaacagcgac aagctcatcg ccagaaagaa ggactgggac ccaaagaagt acggcgggtt
    10981 cgacagccct accgtggcct actcggtcct ggttgtggcg aaggttgaga agggcaagtc
    11041 caagaagctc aagagcgtga aggagctcct ggggatcacc atcatggaga ggtccagctt
    11101 cgagaagaac ccaatcgact tcctggaggc caagggctac aaggaggtga agaaggacct
    11161 gatcatcaag ctcccgaagt actctctctt cgagctggag aacggcagga agagaatgct
    11221 ggcttccgct ggcgagctcc agaaggggaa cgagctcgcg ctgccaagca agtacgtgaa
    11281 cttcctctac ctggcttccc actacgagaa gctcaagggc agcccggagg acaacgagca
    11341 aaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcatcg agcaaatctc
    11401 cgagttcagc aagcgcgtga tcctcgccga cgcgaacctg gataaggtcc tctccgccta
    11461 caacaagcac cgggacaagc ccatcagaga gcaagcggag aacatcatcc atctcttcac
    11521 cctgacgaac ctcggcgctc ctgctgcttt caagtacttc gacaccacga tcgatcggaa
    11581 gagatacacc tccacgaagg aggtcctgga cgcgaccctc atccaccagt cgatcaccgg
    11641 cctgtacgag acgaggatcg acctctcaca actcggcggg gataagagac ccgcagcaac
    11701 caagaaggca gggcaagcaa agaagaagaa gtgactcgag tatatgaaga tgaagatgaa
    11761 atatttggtg tgtcaaataa aaagcttgtg tgcttaagtt tgtgtttttt tcttggcttg
    11821 ttgtgttatg aatttgtggc tttttctaat attaaatgaa tgtaagatca cattataatg
    11881 aataaacaaa tgtttctata atccattgtg aatgttttgt tggatctctt ctgcagcata
    11941 taactactgt atgtgctatg gtatggacta tggaatatga ttaaagataa ggagctccgg
    12001 tgacggacgg cgcgccgaag agcacgtcag tgtttggttt ccactagcac gagtagcgca
    12061 atcagaaaat tttcaatgca tgaagtacta aacgaagttt atttagaaat ttttttaaga
    12121 aatgagtgta attttttgcg acgaatttaa tgacaataat taatcgatga ttgcctacag
    12181 taatgctaca gtaaccaacc tctaatcatg cgtcgaatgc gtcattagat tcgtctcgca
    12241 aaatagcaca agaattatga aattaatttt acaaactatt tttatttaat actaataatt
    12301 aactgtcaaa gtttgtgcta ctcgcaagag tagcgcgaac caaacacggc ctggaggagc
    12361 acggtaacgg cgtcgacaaa ctaacggcca ccacccgcca acgcaaagga gacggatgag
    12421 agttgacttc ttgacggttc tccacccctc tgtctctctg tcactgggcc ctgggtcccc
    12481 ctctcgaaag ttcctctggc cgaaattgcg cggcggagac gaggcgggcg gaaccgtcac
    12541 ggcagaggat tccttcccca ccctgcctgg cccggccata tataaacagc caccgcccct
    12601 ccccgttccc catcgcgtct cgtctcgtgt tgttcccaga acacaaccaa aatccaaatc
    12661 ctcctcctcc tcccgagcct cgtcgatccc tcacccgctt caaggtacgg cgatcctcct
    12721 ctcccttctc ccctcgatcg attatgcgtg ttccgtttcc gtttccgatc gagcgaatcg
    12781 atggttagga cccatggggg acccatgggg tgtcgtgtgg tggtctggtt tgatccgcga
    12841 tatttctccg ttcgtagtgt agatctgatc gaatccctgg tgaaatcgtt gatcgtgcta
    12901 ttcgtgtgag ggttcttagg tttggagttg tggaggtagt tctgatcggt ttgtaggtga
    12961 gattttcccc atgattttgc ttggctcgtt tgtcttggtt agattagatc tgcccgcatt
    13021 ttgttcgata tttctgatgc agatatgatg aataatttcg tccttgtatc ccgcgtccgt
    13081 atgtgtatta agtttgcagg tcctagttag gtttttccta ctgatttgtc ttatccattc
    13141 tgtttagctt gcaaggtttg gtaatggtcc ggcatgtttg tctctataga ttagagtaga
    13201 ataagattat ctcaacaagc tgttggctta tcaattttgg atctgcatgt gtttcgcatc
    13261 tatatctttg caattaagat ggtagatgga catatgctcc tgttgagttg atgttgtacc
    13321 ttttacctga ggtctgagga acatgcatcc tcctgctact ttgtgcttat acagatcatc
    13381 aagattatgt agctaatatt cgatcagttt ctagtatcta catggtaaac ttgcatgcac
    13441 ttgctactta tttttgatat acttggatga taacatatgc tgctggttga ttcctaccta
    13501 catgatgaac attttacagg ccattagtgt ctgtctgtat gtgttgttcc tgtttgcttc
    13561 agtctatttc tgtttcattc ctagtttatt ggttctctgc tagatactta ccctgctggg
    13621 cttagttatc atcttatctc gaatgcattt tcatgtttat agatgaatat acactcagat
    13681 aggtgtagat gtatgctact gtttctctac gttgctgtag gttttacctg tggcaactgc
    13741 atactcctgt tgcttcgcta gatatgtatg tgcttatata gattaagata tgtgtgatgg
    13801 ttctttagta tatctgatga tcatgtatgc tcttttaact tcttgctaca cttggtaaca
    13861 tgctgtgatg ctgtttgttg attctgtagc actaccaatg atgaccttat ctctctttgt
    13921 atatgatgtt tctgtttgtt tgaggcttgt gttactgcta gttacttacc ctgttgcctg
    13981 gctaatcttc tgcagatcct gcaggttcac tgccgtatag gcaggtttta gagctagaaa
    14041 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
    14101 gttcactgcc gtataggcag aagccagata acagtatgcg tatttgcgcg ctgatttttg
    14161 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaggag gtatgctatg
    14221 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
    14281 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
    14341 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
    14401 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
    14461 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
    14521 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
    14581 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
    14641 atggccagtg tgccggtttc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
    14701 aatgacatca aaaacgccat taacctgatg ttctggggaa tataagctct tcagtcgatc
    14761 gacaagctcg agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt
    14821 tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt
    14881 tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat
    14941 ccagatcccc cgaattaccg gtggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    15001 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    15061 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    15121 gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
    15181 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
    15241 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
    15301 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
    15361 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
    15421 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
    15481 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
    15541 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
    15601 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
    15661 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
    15721 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
    15781 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
    15841 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
    15901 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
    15961 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
    16021 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
    16081 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
    16141 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
    16201 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
    16261 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg gctcaaccgt
    16321 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
    16381 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
    16441 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
    16501 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
    16561 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
    16621 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
    16681 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
    16741 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
    16801 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
    16861 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
    16921 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
    16981 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
    17041 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
    17101 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
    17161 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
    17221 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
    17281 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
    17341 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
    17401 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
    17461 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
    17521 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
    17581 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
    17641 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
    17701 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
    17761 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
    17821 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
    17881 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
    17941 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
    18001 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
    18061 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
    18121 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
    18181 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
    18241 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
    18301 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    18361 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    18421 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    18481 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    18541 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    18601 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    18661 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt
    18721 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    18781 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    18841 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    18901 aactgtctgg ccagcgcaca gcccaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    18961 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    19021 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    19081 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    19141 tgacacatgc agctcccgga aacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    19201 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    19261 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    19321 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    19381 tcaggccctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    19441 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    19501 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    19561 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    19621 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    19681 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    19741 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    19801 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    19861 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    19921 cagccactgg taacaggatt agcagagcga ggtat
//
