LOCUS       sequence-182287-        9188 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant 
            Selection: nos:hpt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_37J
FEATURES             Location/Qualifiers
     source          1..9188
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..915)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(100..915)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     823..842
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     907..926
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1206..1641
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    1788..1812
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1835..2087)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(3202..3385)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(3238..3417)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3996..4341
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4284..4304
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4463..4528
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4535..4555
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4994..5299)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(5096..5115)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6373..6429
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6433..6498
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6505..6525
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6853..7260)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     7110..7132
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7124..7141
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7125..7141
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7151..7170
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7151..7169
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7217..7237)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7217..7235)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7256..7272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7256..7272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7269..7291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7280..7296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7304..7310,7311..7328,7329..7334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7349..7370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7556..8143
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      8157..8405
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    8412..8436
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(8529..9117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8609..8628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
       61 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      121 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      181 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      241 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      301 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      361 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      421 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gccgaaatac
      481 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      541 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      601 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      661 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      721 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      781 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      841 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
      901 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      961 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1021 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1081 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1141 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1201 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1261 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     1321 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     1381 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     1441 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     1501 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     1561 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     1621 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     1681 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     1741 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcctgg caggatatat
     1801 tgtggtgtaa acgtgtacgc gttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
     1861 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     1921 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     1981 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     2041 acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg gaaattcggg
     2101 ggatcagctt gtgatcatgt tacatcacct ccaagatccc ggacgagtgc tggggcgtcg
     2161 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
     2221 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
     2281 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
     2341 accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg gatgcctccg
     2401 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
     2461 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
     2521 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
     2581 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
     2641 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
     2701 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
     2761 cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg gctgcagaac
     2821 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat
     2881 gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa tcgggagcgc
     2941 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
     3001 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
     3061 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac
     3121 agacgtcgcg gtgagttcag gctttttcat atctcattgc cccccgggat cttgcaggtc
     3181 gagatcctct agcggatccg gtgcagatta tttggattga gagtgaatat gagactctaa
     3241 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     3301 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     3361 ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt
     3421 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc
     3481 ttaattctcc gctcatgatc ggcgcgccag atttgccttt tcaatttcag aaagaatgct
     3541 aacccacaga tggttagaga ggcttacgca gcaggtatca tcaagacgat ctacccgagc
     3601 aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     3661 aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt
     3721 ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc
     3781 tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac
     3841 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
     3901 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
     3961 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
     4021 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
     4081 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     4141 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     4201 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     4261 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     4321 tcatttcatt tggagagaac acgggggact cctgcaggga attccaagca acgaactgcg
     4381 agtgattcaa gaaaaaagaa aacctgagct ttcgatctct tcggagtggt ttcttgttct
     4441 ttgaaaaaga gggggaagca tggattataa ggatcacgat ggcgactaca aggaccacga
     4501 tattgactac aaagacgatg acgataaaat ggctcctaag aaaaagcgca aagtcggtat
     4561 ccatggcgtt ccctctagaa tggtggatct acgcacgctc ggctacagtc agcagcagca
     4621 agagaagatc aaaccgaagg tgcgttcgac agtggcgcag caccacgagg cactggtggg
     4681 ccatgggttt acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac
     4741 cgtcgctgtc acgtatcagc acataatcac ggcgttgcca gaggcgacac acgaagacat
     4801 cgttggcgtc ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc tcacggatgc
     4861 gggggagttg agaggtccgc cgttacagtt ggacacaggc caacttgtga agattgcaaa
     4921 acgtggcggc gtgaccgcaa tggaggcagt gcatgccagc aggaatgcgt taacaggtgc
     4981 ccccctggag acgttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt
     5041 cgcggtggct gagatcagcc acttcttccc cgataacgga aaccggcaca ctggccatat
     5101 cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg
     5161 agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg
     5221 tgtcaataat atcactctgt acatccacaa acagacgata acggctctct cttttatagg
     5281 tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt
     5341 tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca
     5401 cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca
     5461 tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca
     5521 tagcatacct ccttttgaca tacttcgggt atacatatca gtatatattc ttataccgca
     5581 aaaatcagcg cgcaaatacg catactgtta tctggcttcg tctccaacga ccacctcgtc
     5641 gccttggcct gcctcggcgg acgtcctgcc atggatgcag tgaaaaaggg attgccgcac
     5701 gcgccggaat tgatcagaag agtcaatcgc cgtattggcg aacgcacgtc ccatcgcgtt
     5761 gccggatccc agctggtgaa gtccgagctg gaagaaaaaa agagcgagct gcgccacaag
     5821 ctcaagtacg tgccccacga gtacatcgag ctgatcgaga tcgcccgcaa cagcacccaa
     5881 gaccgcatcc tggagatgaa agtgatggag ttcttcatga aggtgtacgg ctaccgcggc
     5941 aagcacctgg gcggctcccg caagcccgat ggcgccatct acaccgtggg ctcccccatc
     6001 gactatggcg tcattgtcga caccaaggcc tactccggcg gctacaactt acccatcggt
     6061 caggccgacg agatgcaacg ctacgtgaag gagaaccaga cccgcaataa gcacattaat
     6121 cccaacgagt ggtggaaggt gtacccctcc tccgtgaccg agttcaaatt cctgttcgtg
     6181 tccggccact tcaagggcaa ttataaggcc caactgaccc gcctgaaccg caagaccaac
     6241 tgcaacggcg ccgtgctgtc cgtggaggaa ctgctgatcg gcggcgagat gatcaaggct
     6301 ggtaccctga ccctggaaga ggtgcgccgc aagttcaaca atggtgaaat caatttcagg
     6361 tccggaagcg gcgccacgaa cttctctctg ttaaagcaag caggtgacgt ggaagaaaac
     6421 ccagggccca tggactacaa agaccatgac ggtgattata aagatcatga catcgattac
     6481 aaggatgacg atgacaagat ggcccccaag aagaagagga aggtgggcat tcacggggtg
     6541 ccggctagca tggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc
     6601 aaaccgaagg tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt
     6661 acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc
     6721 acgtatcagc acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc
     6781 ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc tcacggatgc gggggagttg
     6841 agaggtccgc cgttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc
     6901 gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc ccccctggag
     6961 accggcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     7021 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7081 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     7141 tgagcgcgcg taatacgact cactataggg cgaattgggt accgggccgc cccttgaggt
     7201 cctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     7261 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7321 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7381 gctcactgcc cgctttccac cggtggtctc caacgatcat ctggtggcac tcgcgtgcct
     7441 cggcggacgt cctgccatgg atgcagtgaa aaagggattg ccgcacgcgc cggaattgat
     7501 cagaagagtc aatcgccgta ttggcgaacg cacgtcccat cgcgttgcca gatctcaact
     7561 agtcaaaagt gaactggagg agaagaaatc tgaacttcgt cataaattga aatatgtgcc
     7621 tcatgaatat attgaattaa ttgaaattgc cagaaattcc actcaggata gaattcttga
     7681 aatgaaggta atggaatttt ttatgaaagt ttatggatat agaggtaaac atttgggtgg
     7741 atcaaggaaa ccggacggag caatttatac tgtcggatct cctattgatt acggtgtgat
     7801 cgtggatact aaagcttata gcggaggtta taatctgcca attggccaag cagatgaaat
     7861 ggagcgatat gtcgaagaaa atcaaacacg agacaaacat ctcaacccta atgaatggtg
     7921 gaaagtctat ccatcttctg taacggaatt taagttttta tttgtgagtg gtcactttaa
     7981 aggaaactac aaagctcagc ttacacgatt aaatcatatc actaattgta atggagctgt
     8041 tcttagtgta gaagagcttt taattggtgg agaaatgatt aaagccggca cattaacctt
     8101 agaggaagtg aggcggaaat ttaataacgg cgagataaac ttttaatagc tcgagatatg
     8161 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     8221 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     8281 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     8341 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     8401 ataaggagct ctgacaggat atattggcgg gtaaactaag tcgctgtatg tgtttgtttg
     8461 atttaaatca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8941 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9001 ccagttacct tcggaagaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9181 attttggt
//
