LOCUS       sequence-182288-        8650 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant 
            Selection: nos:bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8650)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pDIRECT_39J
FEATURES             Location/Qualifiers
     source          1..8650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..915)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(100..915)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     823..842
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     907..926
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1206..1641
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    1788..1812
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1835..2087)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2107..2658)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2700..2879)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3458..3803
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3746..3766
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3925..3990
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3997..4017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(4456..4761)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(4558..4577)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             5835..5891
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5895..5960
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5967..5987
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(6315..6722)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     6572..6594
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6586..6603
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6587..6603
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6613..6632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6613..6631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6679..6699)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6679..6697)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6718..6734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6718..6734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6731..6753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6742..6758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6766..6772,6773..6790,6791..6796))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6811..6832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             7018..7605
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMER
                     YVEENQTRDKHLNPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     terminator      7619..7867
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    7874..7898
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7991..8579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8071..8090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
       61 atcaatctaa agtatatatg tgtaacattg gtctagtgat tagaaaaact catcgagcat
      121 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
      181 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
      241 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
      301 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
      361 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
      421 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gccgaaatac
      481 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
      541 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
      601 tgttttccct gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
      661 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
      721 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
      781 cccatacaat cggtagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
      841 cccatataaa tcagcatcca tgttggaatt taatcgcggc cttgagcaag acgtttcccg
      901 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
      961 tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacaacgtg
     1021 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1081 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1141 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1201 gatccccatc caacagcccg ccgtcgagcg ggctttttta tccccggaag cctgtggata
     1261 gagggtagtt atccacgtga aaccgctaat gccccgcaaa gccttgattc acggggcttt
     1321 ccggcccgct ccaaaaacta tccacgtgaa atcgctaatc agggtacgtg aaatcgctaa
     1381 tcggagtacg tgaaatcgct aataaggtca cgtgaaatcg ctaatcaaaa aggcacgtga
     1441 gaacgctaat agccctttca gatcaacagc ttgcaaacac ccctcgctcc ggcaagtagt
     1501 tacagcaagt agtatgttca attagctttt caattatgaa tatatatatc aattattggt
     1561 cgcccttggc ttgtggacaa tgcgctacgc gcaccggctc cgcccgtgga caaccgcaag
     1621 cggttgccca ccgtcgagcg cctttgccca caacccggcg gccggccgca acagatcgtt
     1681 ttataaattt ttttttttga aaaagaaaaa gcccgaaagg cggcaacctc tcgggcttct
     1741 ggatttccga tccccggaat tagatccgtt taaactacgt aagatcctgg caggatatat
     1801 tgtggtgtaa acgtgtacgc gttgcatgcc ggtcgatcta gtaacataga tgacaccgcg
     1861 cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat
     1921 aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta
     1981 attattacat gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca
     2041 acaggattca atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag
     2101 gggtcatcag atttcggtga cgggcaggac cggacggggc ggcaccggca ggctgaagtc
     2161 cagctgccag aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat
     2221 gccgcggggg gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc
     2281 gatgacagcg accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta
     2341 gagcgtggag cccagtcccg tccgctggtg gcgggggctg acgtacacgg tggactcggc
     2401 cgtccagtcg taggcgttgc gtgccttcca gggacccgcg taggcgatgc cggcgacctc
     2461 gccgtccacc tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt
     2521 ccactcctgc ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg
     2581 gttgacgatg gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg
     2641 gcgtcgttct gggctcatgg tagatcccct cgatcgagtt gagagtgaat atgagactct
     2701 aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca agaaatattt
     2761 gctagatgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt
     2821 gcttagctca ttaaactcca gaaacccgcg gctcagtggc tccttcaacg ttgcggttct
     2881 gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc
     2941 ccttaattct ccgctcatga tcggcgcgcc agatttgcct tttcaatttc agaaagaatg
     3001 ctaacccaca gatggttaga gaggcttacg cagcaggtat catcaagacg atctacccga
     3061 gcaataatct ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat
     3121 tcaggactaa ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta
     3181 ttccagtatg gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag
     3241 tctctaaaaa ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg
     3301 acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca
     3361 atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa
     3421 atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa
     3481 tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag
     3541 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg
     3601 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     3661 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     3721 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     3781 gttcatttca tttggagaga acacggggga ctcctgcagg gaattccaag caacgaactg
     3841 cgagtgattc aagaaaaaag aaaacctgag ctttcgatct cttcggagtg gtttcttgtt
     3901 ctttgaaaaa gagggggaag catggattat aaggatcacg atggcgacta caaggaccac
     3961 gatattgact acaaagacga tgacgataaa atggctccta agaaaaagcg caaagtcggt
     4021 atccatggcg ttccctctag aatggtggat ctacgcacgc tcggctacag tcagcagcag
     4081 caagagaaga tcaaaccgaa ggtgcgttcg acagtggcgc agcaccacga ggcactggtg
     4141 ggccatgggt ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg
     4201 accgtcgctg tcacgtatca gcacataatc acggcgttgc cagaggcgac acacgaagac
     4261 atcgttggcg tcggcaaaca gtggtccggc gcacgcgccc tggaggcctt gctcacggat
     4321 gcgggggagt tgagaggtcc gccgttacag ttggacacag gccaacttgt gaagattgca
     4381 aaacgtggcg gcgtgaccgc aatggaggca gtgcatgcca gcaggaatgc gttaacaggt
     4441 gcccccctgg agacgttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt
     4501 ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gaaaccggca cactggccat
     4561 atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg
     4621 ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg
     4681 cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct ctcttttata
     4741 ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca
     4801 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
     4861 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat
     4921 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
     4981 catagcatac ctccttttga catacttcgg gtatacatat cagtatatat tcttataccg
     5041 caaaaatcag cgcgcaaata cgcatactgt tatctggctt cgtctccaac gaccacctcg
     5101 tcgccttggc ctgcctcggc ggacgtcctg ccatggatgc agtgaaaaag ggattgccgc
     5161 acgcgccgga attgatcaga agagtcaatc gccgtattgg cgaacgcacg tcccatcgcg
     5221 ttgccggatc ccagctggtg aagtccgagc tggaagaaaa aaagagcgag ctgcgccaca
     5281 agctcaagta cgtgccccac gagtacatcg agctgatcga gatcgcccgc aacagcaccc
     5341 aagaccgcat cctggagatg aaagtgatgg agttcttcat gaaggtgtac ggctaccgcg
     5401 gcaagcacct gggcggctcc cgcaagcccg atggcgccat ctacaccgtg ggctccccca
     5461 tcgactatgg cgtcattgtc gacaccaagg cctactccgg cggctacaac ttacccatcg
     5521 gtcaggccga cgagatgcaa cgctacgtga aggagaacca gacccgcaat aagcacatta
     5581 atcccaacga gtggtggaag gtgtacccct cctccgtgac cgagttcaaa ttcctgttcg
     5641 tgtccggcca cttcaagggc aattataagg cccaactgac ccgcctgaac cgcaagacca
     5701 actgcaacgg cgccgtgctg tccgtggagg aactgctgat cggcggcgag atgatcaagg
     5761 ctggtaccct gaccctggaa gaggtgcgcc gcaagttcaa caatggtgaa atcaatttca
     5821 ggtccggaag cggcgccacg aacttctctc tgttaaagca agcaggtgac gtggaagaaa
     5881 acccagggcc catggactac aaagaccatg acggtgatta taaagatcat gacatcgatt
     5941 acaaggatga cgatgacaag atggccccca agaagaagag gaaggtgggc attcacgggg
     6001 tgccggctag catggtggat ctacgcacgc tcggctacag tcagcagcag caagagaaga
     6061 tcaaaccgaa ggtgcgttcg acagtggcgc agcaccacga ggcactggtg ggccatgggt
     6121 ttacacacgc gcacatcgtt gcgctcagcc aacacccggc agcgttaggg accgtcgctg
     6181 tcacgtatca gcacataatc acggcgttgc cagaggcgac acacgaagac atcgttggcg
     6241 tcggcaaaca gtggtccggc gcacgcgccc tggaggcctt gctcacggat gcgggggagt
     6301 tgagaggtcc gccgttacag ttggacacag gccaacttgt gaagattgca aaacgtggcg
     6361 gcgtgaccgc aatggaggca gtgcatgcat cgcgcaatgc actgacgggt gcccccctgg
     6421 agaccggcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     6481 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     6541 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     6601 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc gccccttgag
     6661 gtcctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     6721 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6781 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6841 gcgctcactg cccgctttcc accggtggtc tccaacgatc atctggtggc actcgcgtgc
     6901 ctcggcggac gtcctgccat ggatgcagtg aaaaagggat tgccgcacgc gccggaattg
     6961 atcagaagag tcaatcgccg tattggcgaa cgcacgtccc atcgcgttgc cagatctcaa
     7021 ctagtcaaaa gtgaactgga ggagaagaaa tctgaacttc gtcataaatt gaaatatgtg
     7081 cctcatgaat atattgaatt aattgaaatt gccagaaatt ccactcagga tagaattctt
     7141 gaaatgaagg taatggaatt ttttatgaaa gtttatggat atagaggtaa acatttgggt
     7201 ggatcaagga aaccggacgg agcaatttat actgtcggat ctcctattga ttacggtgtg
     7261 atcgtggata ctaaagctta tagcggaggt tataatctgc caattggcca agcagatgaa
     7321 atggagcgat atgtcgaaga aaatcaaaca cgagacaaac atctcaaccc taatgaatgg
     7381 tggaaagtct atccatcttc tgtaacggaa tttaagtttt tatttgtgag tggtcacttt
     7441 aaaggaaact acaaagctca gcttacacga ttaaatcata tcactaattg taatggagct
     7501 gttcttagtg tagaagagct tttaattggt ggagaaatga ttaaagccgg cacattaacc
     7561 ttagaggaag tgaggcggaa atttaataac ggcgagataa acttttaata gctcgagata
     7621 tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg
     7681 tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta
     7741 agatcacatt ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga
     7801 tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa
     7861 agataaggag ctctgacagg atatattggc gggtaaacta agtcgctgta tgtgtttgtt
     7921 tgatttaaat catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8401 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8461 agccagttac cttcggaaga agagttggta gctcttgatc cggcaaacaa accaccgctg
     8521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8641 ggattttggt
//
