LOCUS       sequence-182290-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This E. coli DH10B strain harbors a shuttle vector plasmid that 
            expresses the Mixed Feedback Loop version of the UBER system with a 
            T7RNAP translation rate of 1535 and a TetR translation rate of 
            30709.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Kushwaha M, Salis HM
  TITLE     A portable expression resource for engineering cross-species genetic
            circuits and pathways.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832.
  PUBMED    26184393
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7832. doi"; pages: " 
            10.1038/ncomms8832"
COMMENT     Sequence Label: MK1283
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttggaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
       61 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      181 tgttgaatac tcatactctt ccaaacgacg gccagtgaat tggcgaattg gagctattaa
      241 ttaaacaaaa aacccctacc cccccgatat gtatttattg cgaaaaaacc ccgccgaagc
      301 ggggtttttt gcgaccggtt attatgaacc tgattcacat tttaactgtt tctccaaacc
      361 gcaaatgatt aattctaaac caaataaaaa tgcaggttct gcaccttgat gatcaaataa
      421 ttcaattgct tgacgtaata aaggaggcat tgaatctgtt gtaggtgttt ctctttcctc
      481 cttggcaact tgatgttctt gatcttctaa aacacaacct aaggtaaaat gaccaactgc
      541 tgataatgca tataatgcgt tttctaatga aaaaccttgt tgacataaga atgctaactg
      601 attttctaat gtttcatatt gtttttctgt agacgtgtac ctaagtgaac tttggcacca
      661 tctctatgtg ataataatgc acaacgaaat gatttggcat tgttacgtaa gaagtcttgc
      721 catgattctc cttctaatgg acaaaaatgt gtatgatgtc tatctaacat ttcgattgct
      781 aatgcatcta a
//
