LOCUS       sequence-182291-         652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This E. coli DH10B strain harbors a shuttle vector plasmid that 
            expresses the Mixed Feedback Loop version of the UBER system with a 
            T7RNAP translation rate of 1984 and a TetR translation rate of 
            3877..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Kushwaha M, Salis HM
  TITLE     A portable expression resource for engineering cross-species genetic
            circuits and pathways.
  JOURNAL   Nat Commun. 2015 Jul 17;6:7832. doi: 10.1038/ncomms8832.
  PUBMED    26184393
REFERENCE   2  (bases 1 to 652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-07-17"; volume: "6:7832. doi"; pages: " 
            10.1038/ncomms8832"
COMMENT     Sequence Label: MK1278
FEATURES             Location/Qualifiers
     source          1..652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(195..299)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttggaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
       61 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      121 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      181 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      241 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      301 acatttcccc gaaaagtgcc acctgggtcc ttttcatcac gtgctataaa aataattata
      361 atttaaattc gttttctaat gaaaaacctt gttgacataa gaatgctaac tgattttcta
      421 atgtttcata ttgtttttct gtaggacgtg tacctaagtg aactttggca ccatctctat
      481 gtgataataa tgcacaacga aatgatttgg cattgttacg taagaagtct tgccatgatt
      541 ctccttctaa tggacaaaaa tgtgtatgat gtctatctaa catttcgatt gctaatgcat
      601 ctaataatgc acgtttgttt ttaacatgcc aatataatgt agtgttcaac ac
//
