LOCUS       sequence-182293-        9823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Cdc25C and N-terminally tagged NBir[2-256]-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Cdc25C WT_Myc-Nbir-GFP
FEATURES             Location/Qualifiers
     source          1..9823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(774..821)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(2548..2571)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2683..2721)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(2736..2754,2755..2777,2778..2796,2797..2819,2820..2838,
                     2839..2861,2862..2880,2881..2903,2904..2922,2923..2945,
                     2946..2964,2965..2987,2988..3006)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        3023..3060
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3158..3187
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(4016..4018,4019..4021,4022..4732)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYDSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4061..4082)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4322..4341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4669..4690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4925..5059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5003..5022
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(5167..5200)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(5253..5269)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5285..5307)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5718..6075
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6140..6609
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7139..7155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7139..7155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7152..7174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7163..7179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7187..7193,7194..7211,7212..7217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7232..7253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7370..7387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7541..8129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7621..7640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8300..9091,9092..9160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8923..8942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9161..9265)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9333..9351
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9389..9411)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9511..9530
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9724..9746
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9738..9755
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9739..9755
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taccaaaaat gatagctcat acctgtaatc ttagatgtgg gaaggctaaa acagcaggct
       61 acccatgact tcaaggtcac cctgggttac agagtagcta atagtctagc ttgaactata
      121 gtgtaggatc tttaaaaaaa aattaaccaa ccaaccaaca aacaaacaaa caaacaacaa
      181 caacaacaaa tggaagacag aggacaaaaa aaaataaaag aaaaagtaga aagccaatgg
      241 agatactatc ttaaaattag aaatatttat gacacattct gaatactcca tgtttatttc
      301 aaggaagcca atgtaacttt aagtactatg gccatttaat gtaaatactt aagaaaaaaa
      361 accaaattaa ttttgataca tgctgcatgt gaaagacccc cgctgacggg tagtcaatca
      421 ctcagaggag accctcccaa ggaacagcga gaccacaagt cggatgcaac tgcaagaggg
      481 tttattggat acacgggtac ccgggcgact cagtcaatcg gaggactggc gccccgagtg
      541 aggggttgtg ggctcgagct cggggagcag aagcgcgcga acagaagtcg agctagcttg
      601 ccaaacctac aggtggggtc tttcattccc ccctttttct ggagactaaa taaaatcttt
      661 tattttatct atggctcgta ctctataggc ttcagctggt gatattgttg agtcaaaact
      721 agagcctgga ccactgatat cctgtcttta acaaattgga ctaatccgga tctgaagttc
      781 ctatactttc tagagaatag gaacttcgga ataggaactt cgcggccaga tcctcgttat
      841 taatcgctat ttaattaatg ggctcatgtc cttcaccaga agggcaatct gctcccgcag
      901 ctgccgctcc ccttcctgca ctttgctctg gcttcgacac ctcagcaact cagtcttgtg
      961 gtcctgatga tgcatagggc agtagctctg tggttcacac agttccatat attctggaaa
     1021 gaagtctctg tagccgcctt taaggatata tagctctggg tagtacaatg caggatactg
     1081 gttcagagac ctgtcctctt cacgcagaca gcggcacatt cgggggcccc tctctgagga
     1141 gaattcacag tggaacacga tgattattct cttctgggtg tccaaaggga cgatgggctt
     1201 cttcagaaag aagttaaaca gttcttcctg actatataag tttaaggctc cctggatgtg
     1261 tcctcccaga tactcatatg gatagcgaca atcaatgaca taaaacttct caatcagacc
     1321 ctggaacttc cccgacagta aggcagccac tgtttctggg ttgacatact tcagatcttg
     1381 gtgtttccct gacacggttg gcagcgcaca taccttggaa aaatcaccaa tcaggtgccc
     1441 ctggttagaa tcttcctcca gcatctgagt gatagtaatg tcacacagag agactgtctt
     1501 ctttaaacat aagcccttcc tgagctttcc ttggccagaa aaatactttt ttttaacttt
     1561 atctggtatt gtgttgtcct tgaatttttc cacctgcttc agtcttggcc tgttcaagtt
     1621 ctctggcatc gacggggagc gatataggcc acttctgctc acctttgctt cttgatcttt
     1681 cagggaaaac tccattaatt catctgaaat ctcttctgcc tggtcttctc ctaggtttgg
     1741 atttttatcc aattttggaa cagtagtaat gggactgccc aaatatttca tttcactgtc
     1801 caccaagttt ccattgtcat tttctttatt tgcagatgaa ctacacattg catctctctt
     1861 tctatggcca cggtccaaac cattcggagt gctacaaaga agctgtgctg ggctacattt
     1921 cattaggtgc tggtcatgat tcatcccagc taaatgcact tcctgaagtc ctgaagaatc
     1981 caggtgacca gtttcatcaa ggtctgcaga agtggtaagc tgagtggcag ttatctcccc
     2041 actgctaaga ttcgaaagat cgaggcaaca ttttggggtt cctccagaca aaatgcttag
     2101 gtttgcagaa tcaccaagaa atttgcccac tggagttcta gggacatctg gacagacggt
     2161 aaaggaagtg tctctctcca ggagcaggtt taacattttc ctttgattag acctaaaact
     2221 gggtcctgag ccagagcttc cttcctctct tgtggatgag aagagttccg tagaggacat
     2281 gaaacaaacg ccgccgccgg aggagccgcc gccccgggag gcgtaggaga tctggttcgc
     2341 ctcgagcttc tctgcgcttc tcagggagat ttctccgccc atccagggct tgatgattcc
     2401 gtcctgctcc agcagcagag ctccctgctt gtcgattcct ctggagatgc cgaagatttc
     2461 cttgtcgccg atgatcagct tcacaggtct gttgatgaag ttgtccagct tctcccatct
     2521 gctcaggtaa ggagccagtc cgttaatctt gtcatcgtcg tccttgtagt ccataattga
     2581 ggccggccga attccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
     2641 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatataggc
     2701 ctcccaccgt acacgcctac cctcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2941 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     3001 tagagaaaag tgaaagaagc ttgtaggcgt gtacggtggg aggcctatat aagcagagct
     3061 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     3121 agacaccggg accgatccag cctccgcggt cgagatggaa caaaaactca tctcagaaga
     3181 ggatctcgac aaggacaaca ccgtgcccct gaagctgatc gccctgctgg ccaacggcga
     3241 gttccactct ggcgagcagc tgggagagac cctgggaatg agcagagccg ccatcaacaa
     3301 gcacatccag acactgagag actggggagt ggacgtgttc accgtgcctg gcaagggcta
     3361 cagcctgcct gagcctatcc agctgctgaa cgccaagcag atcctgggac agctggatgg
     3421 cggaagcgtg gccgtgctgc ctgtgatcga ctccaccaat cagtacctgc tggacagaat
     3481 cggagagctg aagtccggcg acgcctgcat cgccgagtac cagcaggctg gcagaggagg
     3541 cagaggacgg aagtggttca gcccattcgg agccaacctg tacctgtcca tgttctggag
     3601 actggagcag ggacctgctg ctgccatcgg actgagtctg gtgatcggaa tcgtgatggc
     3661 cgaggtgctg agaaagctgg gagccgacaa ggtgagagtg aagtggccta atgacctgta
     3721 cctccaggac cgcaagctgg ctggcatcct ggtggagctg acaggcaaga caggcgatgc
     3781 cgctcagatc gtgatcggag ccggaatcaa catggccatg agaagagtgg aggagagcgt
     3841 ggtgaaccag ggctggatca ccctgcagga ggctggcatc aacctggacc ggaacaccct
     3901 ggccgccatg ctgatcagag agctgagagc cgctctggag ctgttcgagc aggagaaaca
     3961 tggcggcggc tcctccggcg gcggccagat ctcctacgcc tcccggggca tcgatatggt
     4021 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     4081 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     4141 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     4201 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     4261 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     4321 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     4381 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4441 ggagtacaac tacgacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     4501 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     4561 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4621 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4681 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agccaacgcg
     4741 ttagggatcc agaccacctc ccctgcgagc taagctggac agccaatgac gggtaagaga
     4801 gtgacatttt tcactaacct aagacaggag ggccgtcaga gctactgcct aatccaaaga
     4861 cgggtaaaag tgataaaaat gtatcactcc aacctaagac aggcgcagct tccgagggat
     4921 ttgagatcca gacatgataa gatacattga tgagtttgga caaaccaaaa ctagaatgca
     4981 gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt gccttatttg taaccattat
     5041 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     5101 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     5161 tgatctgaag ttcctatact atttgaagaa taggaacttc ggaataggaa cttcggatct
     5221 catatggcca tatgggggcg cctagagaag gagtgagggc tggataaagg gaggatcgag
     5281 gcggggtcga acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg
     5341 gaggcttagg gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc
     5401 aggtcacgat gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga
     5461 tcttaacctg ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa
     5521 aggttggcca ttctgcagag cagaaggtaa cccaacgtct cttcttgaca tctaccgact
     5581 ggttgtgagc gatccgctcg acatctttcc agtgacctaa ggtcaaactt aagggagtgg
     5641 taacaggcta gcccgggccc atattttcag acaaatacag aaacacagtc agacagagac
     5701 aacacagaac gatgctgcag cagacaagac gcgcggcgcg gcttcggtcc caaaccgaaa
     5761 gcaaaaattc agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat
     5821 atccctcctc taaggggggt gcaccaaaga gtcctaaacg atcgggattt taggactcag
     5881 gtcgggccac aaaaacggcc cccgaagtcc ctgggacgtc tcccagggtt gcggccgggt
     5941 gttccgaact cgtcagttcc accacgggtc cgccagatac agagctagtt agctaactag
     6001 taccgacgca ggcgcataaa atcagtcata gacactagac aatcggacag acacagataa
     6061 gttgctggcc agcttacctc ccggtggtgg gtcggtggtc cctgggcagg ggtctcccga
     6121 tcccggacga gcccccaaat gaaagacccc cgctgacggg tagtcaatca ctcagaggag
     6181 accctcccaa ggaacagcga gaccacaagt cggatgcaac tgcaagaggg tttattggat
     6241 acacgggtac ccgggcgact cagtcaatcg gaggactggc gcgccgagtg aggggttgtg
     6301 ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat
     6361 tggttagttc aaataaggca cagggtcatt tcaggtcctt ggggcaccct ggaaacatct
     6421 gatggttctc tagaaactgc tgagggctgg accgcatctg gggaccatct gttcttggcc
     6481 ctgagccggg gcaggaactg cttaccacag atatcctgtt tggcccatat tcagctgttc
     6541 catctgttct tggccctgag ccggggcagg aactgcttac cacagatatc ctgtttggcc
     6601 catattcagc tgttccatct gttcctgacc ttgatctgaa cttttctatt ctcagttatg
     6661 tatttttcca tgccttgcaa aatggcgtta cttaagctag cttgccaaac ctacaggtgg
     6721 ggtctttcac atgtatatgt caaaaataaa aatcaactaa ttgactagta attaatatga
     6781 ctggcataat gggaaattga tcctgacaga tgcaaactgg cttctcagca gcgcatttat
     6841 gttgtcaact gaggaaggaa acgttaatga cagaaactct aagtaatttc cacgtttatc
     6901 tatttttatt tatactagct ttggtaacag gaatattgca gcattcatgc acattgaaac
     6961 ccttatgaaa taaaaacatc tgtgcattta aaatggaatt aacattttaa atgttaaaaa
     7021 aagctggctt agcttccccc cgccccctag ggcatagaac aagtcaaatg ctttatatat
     7081 ttgagtttgg gatgtattag gaaactccta agagcaaagc tagcttggcg taatcatggt
     7141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7201 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7261 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7321 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7981 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8041 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     9301 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     9361 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     9421 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9481 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9541 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     9601 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9661 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9721 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt aattccaagc taagtgaaag
     9781 actctgtctc aaaaaccaac ctgacacctg agccggagcg ttg
//
