LOCUS       sequence-182297-       15634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-Dicer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 15634)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 15634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
FEATURES             Location/Qualifiers
     source          1..15634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(829..876)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      1558..1567
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3767..3790)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3902..3940)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(3955..3973,3974..3996,3997..4015,4016..4038,4039..4057,
                     4058..4080,4081..4099,4100..4122,4123..4141,4142..4164,
                     4165..4183,4184..4206,4207..4225)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4242..4279
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4377..4406
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    11199..11333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     11277..11296
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(11441..11474)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(11527..11543)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(11559..11581)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    11992..12349
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             12414..12883
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(13413..13429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13413..13429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13426..13448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13437..13453
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13461..13467,13468..13485,13486..13491))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13506..13527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13644..13661)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13815..14403)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13895..13914)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14574..15365,15366..15434))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15197..15216
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15435..15539)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15607..15625
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcacacacac aaacacaatc acacacacac acatcaatca atcaatcaat caatatacca
       61 aaaatgatag ctcatacctg taatcttaga tgtgggaagg ctaaaacagc aggctaccca
      121 tgacttcaag gtcaccctgg gttacagagt agctaatagt ctagcttgaa ctatagtgta
      181 ggatctttaa aaaaaaatta accaaccaac caacaaacaa acaaacaaac aacaacaaca
      241 acaaatggaa gacagaggac aaaaaaaaat aaaagaaaaa gtagaaagcc aatggagata
      301 ctatcttaaa attagaaata tttatgacac attctgaata ctccatgttt atttcaagga
      361 agccaatgta actttaagta ctatggccat ttaatgtaaa tacttaagaa aaaaaaccaa
      421 attaattttg atacatgctg catgtgaaag acccccgctg acgggtagtc aatcactcag
      481 aggagaccct cccaaggaac agcgagacca caagtcggat gcaactgcaa gagggtttat
      541 tggatacacg ggtacccggg cgactcagtc aatcggagga ctggcgcccc gagtgagggg
      601 ttgtgggctc gagctcgggg agcagaagcg cgcgaacaga agtcgagcta gcttgccaaa
      661 cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa tcttttattt
      721 tatctatggc tcgtactcta taggcttcag ctggtgatat tgttgagtca aaactagagc
      781 ctggaccact gatatcctgt ctttaacaaa ttggactaat ccggatctga agttcctata
      841 ctttctagag aataggaact tcggaatagg aacttcgcgg ccagatcctc gttattaatc
      901 gctatttaat taatcaagca aagtacatgg tgcgcagagt gtcttggtga acctggaccg
      961 ccttggccag tgcttggtgg tctcgcccgt tactctgccc agaggtatgg cttccttcag
     1021 cactgtcatg ttccttatcc accaggtggt acctggcccg gaaggccacc aggtgagcgt
     1081 agtatgctgg cgctgggatg gacacggagc gtgtgcagcg cacgtaggtg tgacacagct
     1141 ggtaggttag gatctgcagc tcatcagagg agaaacgatt gtcgtcccag aggacgtgat
     1201 agtgcgaagg cctgcttgtc ccctggatgc cagcgtgact acacaggtag aagtcgaact
     1261 cggtggggtg ggtgattttc gtgtccacag tcgtgcctgc tggaatgttt ccacttttcc
     1321 caacccgctc gttcttgtca gtgcagaaga gccgggtgtg gtgcctcttc tgcaccacga
     1381 tgaaggtgat cccgggctgg tagtcttttt ctagcttgat acaggcctca cggatggcca
     1441 gcaactcgtg gtggagaacc tgctggaact ggccttcaga gacaccgtcg cggtagaaga
     1501 tgatgcgggt gggcttgaag cgcgtggact tgtagaactg gatgaggagc tcgcggacca
     1561 tggcggccag gtcttgtatg atctcctgcc ggtgctgctg cacgcgcacg gtggcgcagt
     1621 agcgattggg gtgggcgtcc atgctgccca ccacggcggc aatggagggc ttcttcccat
     1681 ccccggcggg ggggtgagtg acgtctgctc ccagaaagat gacgggctgc tggaacaccg
     1741 gcggcctgcc ctggggcagc aggatgttgt tcacgcctcc cagcttgacg ttgatcttca
     1801 ggcagaggtt ggacagggtc tgtggcgtgg tcctctgcac gttcttcatc tgcacgcact
     1861 gcgtggccat ccccagcacc gtgtctccca cgcgcttgac ctcggcgtac acgggcgtct
     1921 tgccgggcag gatgaccacc accagctgca ggcccgcata cgtgttcttc aggtgccgga
     1981 acatgggctc cacgctgtcc gccccctgcg cgtatttgca gaagcacggc tggccctgga
     2041 tgggcatgcc ggcgtctctc gagatctttc tgagctgctc tgtgaaggac ttcagatgga
     2101 cttccgtgca ctggcgctgg ggggcgaagc acgcaatggc ccacaccttg atctcgatgc
     2161 ccgtgtggaa ctgcttgttc cgcatgtccc agacgccctg gacaggggtc gcaatagctt
     2221 tattcctgcc cccgtagagg atggagggcg gctgcagcac ccgcccagtc acgtctgtca
     2281 tctcatcttt gaccatgatt ccaaattcac ggacgtatgg atctgtgttg aaacttgcac
     2341 ttcgcatcaa tttgctaatc tcttcttgcc gatcgggcgc cgacctagca gtcgctctga
     2401 tcatggttga ggtctgattg tccgttaatt ttttaataca tctttgtcct gccacaatgt
     2461 tacagacctc caggggaagg taggtgtgtt tctgctcctg tccgacttgt aaacatggga
     2521 ggtgggggta gcgcagaacc aacttgtgcc tgtccttgaa atactgggcc accgtgcact
     2581 ccaccgtctg cccgctctcc tgctgcagcg ggaatgtttg gtgactggcg ggccgccggg
     2641 tcacattgca gacacggtac ttcctcttca tctgcccaca gtgcgttatc tccaccttta
     2701 gacctttaat ttctttggta aactttaccc tttgggaatc tgtcagaggt ttttgttgtt
     2761 cttcaatact tttaaaatcc aaaacttcac aaacaaactc gattactggc tgtgccttgt
     2821 aaaacgctgt tgctgacaca tcaatattca gcatcatttt ccagagagaa ggccggacgg
     2881 actgatggaa gccaaaccac acttctcggc ccccgccaag agggttagag cagccttcgg
     2941 acgcggtgaa gaaggagcgg cccacggggg tgtacctcat ggatggcaag tgcctcatga
     3001 ccacgtccag ggcctggatc gtctcaaaag ggacgctggg cagccgccct gaaagtgcat
     3061 cgtgtaacgc ctgcaagctc acgcaggaca cccacttgat ggacaccttg aagatgcgat
     3121 ccttgccttc tcctggcagc gtgacctcca gctccacctt gtccctccca atcggaaggg
     3181 gcatggctgt gtatagattc ttcctgccgt caaacacggg cttccgatcc ccaaagatct
     3241 gtgttttaaa gtgctggacc atgtgttcca cgatttccct gttaactctc ctcgggcact
     3301 tctctggctt gatatccaat tcataatgat agatgtcaat tttggggatg tccatttcga
     3361 agaaattggc ctgtaatttg attgttctcc cggaggtccc aaagtcgggt ctaggtggag
     3421 gcttgaaggc atatccttgg atggggggcg gcggcgcagg aggtgcaagt gcggggccgg
     3481 ctcccgagta catcatgttt aaacaaacgc cgccgccgga ggagccgccg ccccgggagg
     3541 cgtaggagat ctggttcgcc tcgagcttct ctgcgcttct cagggagatt tctccgccca
     3601 tccagggctt gatgattccg tcctgctcca gcagcagagc tccctgcttg tcgattcctc
     3661 tggagatgcc gaagatttcc ttgtcgccga tgatcagctt cacaggtctg ttgatgaagt
     3721 tgtccagctt ctcccatctg ctcaggtaag gagccagtcc gttaatcttg tcatcgtcgt
     3781 ccttgtagtc cataattgag gccggccgaa ttccgcggag gctggatcgg tcccggtgtc
     3841 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
     3901 gagctctgct tatataggcc tcccaccgta cacgcctacc ctcgagttta ccactcccta
     3961 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     4021 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     4081 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     4141 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     4201 taccactccc tatcagtgat agagaaaagt gaaagaagct tgtaggcgtg tacggtggga
     4261 ggcctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
     4321 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggtc gagatggaac
     4381 aaaaactcat ctcagaagag gatctcgaca aggacaacac cgtgcccctg aagctgatcg
     4441 ccctgctggc caacggcgag ttccactctg gcgagcagct gggagagacc ctgggaatga
     4501 gcagagccgc catcaacaag cacatccaga cactgagaga ctggggagtg gacgtgttca
     4561 ccgtgcctgg caagggctac agcctgcctg agcctatcca gctgctgaac gccaagcaga
     4621 tcctgggaca gctggatggc ggaagcgtgg ccgtgctgcc tgtgatcgac tccaccaatc
     4681 agtacctgct ggacagaatc ggagagctga agtccggcga cgcctgcatc gccgagtacc
     4741 agcaggctgg cagaggaggc agaggacgga agtggttcag cccattcgga gccaacctgt
     4801 acctgtccat gttctggaga ctggagcagg gacctgctgc tgccatcgga ctgagtctgg
     4861 tgatcggaat cgtgatggcc gaggtgctga gaaagctggg agccgacaag gtgagagtga
     4921 agtggcctaa tgacctgtac ctccaggacc gcaagctggc tggcatcctg gtggagctga
     4981 caggcaagac aggcgatgcc gctcagatcg tgatcggagc cggaatcaac atggccatga
     5041 gaagagtgga ggagagcgtg gtgaaccagg gctggatcac cctgcaggag gctggcatca
     5101 acctggaccg gaacaccctg gccgccatgc tgatcagaga gctgagagcc gctctggagc
     5161 tgttcgagca ggagaaacat ggcggcggct cctccggcgg cggccagatc tcctacgcct
     5221 cccggggcat tgagatgaaa agccctgctt tgcaacccct cagcatggca ggcctgcagc
     5281 tcatgacccc tgcttcctca ccaatgggtc ctttctttgg actgccatgg caacaagaag
     5341 caattcatga taacatttat acgccaagaa aatatcaggt tgaactgctt gaagcagctc
     5401 tggatcataa taccatcgtc tgtttaaaca ctggctcagg gaagacattt attgcagtac
     5461 tactcactaa agagctgtcc tatcagatca ggggagactt cagcagaaat ggaaaaagga
     5521 cggtgttctt ggtcaactct gcaaaccagg ttgctcaaca agtgtcagct gtcagaactc
     5581 attcagatct caaggttggg gaatactcaa acctagaagt aaatgcatct tggacaaaag
     5641 agagatggaa ccaagagttt actaagcacc aggttctcat tatgacttgc tatgtcgcct
     5701 tgaatgtttt gaaaaatggt tacttatcac tgtcagacat taaccttttg gtgtttgatg
     5761 agtgtcatct tgcaatccta gaccacccct atcgagaaat tatgaagctc tgtgaaaatt
     5821 gtccatcatg tcctcgcatt ttgggactaa ctgcttccat tttaaatggg aaatgtgatc
     5881 cagaggaatt ggaagaaaag attcagaaac tagagaaaat tcttaagagt aatgctgaaa
     5941 ctgcaactga cctggtggtc ttagacaggt atacttctca gccatgtgag attgtggtgg
     6001 attgtggacc atttactgac agaagtgggc tttatgaaag actgctgatg gaattagaag
     6061 aagcacttaa ttttatcaat gattgtaata tatctgtaca ttcaaaagaa agagattcta
     6121 ctttaatttc gaaacagata ctatcagact gtcgtgccgt attggtagtt ctgggaccct
     6181 ggtgtgcaga taaagtagct ggaatgatgg taagagaact acagaaatac atcaaacatg
     6241 agcaagagga gctgcacagg aaatttttat tgtttacaga cactttccta aggaaaatac
     6301 atgcactatg tgaagagcac ttctcacctg cctcacttga cctgaaattt gtaactccta
     6361 aagtaatcaa actgctcgaa atcttacgca aatataaacc atatgagcga cagcagtttg
     6421 aaagcgttga gtggtataat aatagaaatc aggataatta tgtgtcatgg agtgattctg
     6481 aggatgatga tgaggatgaa gaaattgaag aaaaagagaa gccagagaca aattttcctt
     6541 ctccttttac caacattttg tgcggaatta tttttgtgga aagaagatac acagcagttg
     6601 tcttaaacag attgataaag gaagctggca aacaagatcc agagctggct tatatcagta
     6661 gcaatttcat aactggacat ggcattggga agaatcagcc tcgcaacaaa cagatggaag
     6721 cagaattcag aaaacaggaa gaggtactta ggaaatttcg agcacatgag accaacctgc
     6781 ttattgcaac aagtattgta gaagagggtg ttgatatacc aaaatgcaac ttggtggttc
     6841 gttttgattt gcccacagaa tatcgatcct atgttcaatc taaaggaaga gcaagggcac
     6901 ccatctctaa ttatataatg ttagcggata cagacaaaat aaaaagtttt gaagaagacc
     6961 ttaaaaccta caaagctatt gaaaagatct tgagaaacaa gtgttccaag tcggttgata
     7021 ctggtgagac tgacattgat cctgtcatgg atgatgatga cgttttccca ccatatgtgt
     7081 tgaggcctga cgatggtggt ccacgagtca caatcaacac ggccattgga cacatcaata
     7141 gatactgtgc tagattacca agtgatccgt ttactcatct agctcctaaa tgcagaaccc
     7201 gagagttgcc tgatggtaca ttttattcaa ctctttatct gccaattaac tcacctcttc
     7261 gagcctccat tgttggtcca ccaatgagct gtgtacgatt ggctgaaaga gttgtagctc
     7321 tcatttgctg tgagaaactg cacaaaattg gcgaactgga tgaccatttg atgccagttg
     7381 ggaaagagac tgttaaatat gaagaggagc ttgatttgca tgatgaagaa gagaccagtg
     7441 ttccaggaag accaggttcc acgaaacgaa ggcagtgcta cccaaaagca attccagagt
     7501 gtttgaggga tagttatccc agacctgatc agccctgtta cctgtatgtg ataggaatgg
     7561 ttttaactac acctttacct gatgaactca actttagaag gcggaagctc tatcctcctg
     7621 aagataccac aagatgcttt ggaatactga cggccaaacc catacctcag attccacact
     7681 ttcctgtgta cacacgctct ggagaggtta ccatatccat tgagttgaag aagtctggtt
     7741 tcatgttgtc tctacaaatg cttgagttga ttacaagact tcaccagtat atattctcac
     7801 atattcttcg gcttgaaaaa cctgcactag aatttaaacc tacagacgct gattcagcat
     7861 actgtgttct acctcttaat gttgttaatg actccagcac tttggatatt gactttaaat
     7921 tcatggaaga tattgagaag tctgaagctc gcataggcat tcccagtaca aagtatacaa
     7981 aagaaacacc ctttgttttt aaattagaag attaccaaga tgccgttatc attccaagat
     8041 atcgcaattt tgatcagcct catcgatttt atgtagctga tgtgtacact gatcttaccc
     8101 cactcagtaa atttccttcc cctgagtatg aaacttttgc agaatattat aaaacaaagt
     8161 acaaccttga cctaaccaat ctcaaccagc cactgctgga tgtggaccac acatcttcaa
     8221 gacttaatct gttgacacct cgacatttga atcagaaggg gaaagcgctt cctttaagca
     8281 gtgctgagaa gaggaaagcc aaatgggaaa gtctgcagaa taaacagata ctggttccag
     8341 aactctgtgc tatacatcca attccagcat cactgtggag aaaagctgtt tgtctcccca
     8401 gcatacttta tcgccttcac tgccttttga ctgcagagga gctaagagcc cagactgcca
     8461 gcgatgctgg cgtgggagtc agatcacttc ctgcggattt tagataccct aacttagact
     8521 tcgggtggaa aaaatctatt gacagcaaat ctttcatctc aatttctaac tcctcttcag
     8581 ctgaaaatga taattactgt aagcacagca caattgtccc tgaaaatgct gcacatcaag
     8641 gtgctaatag aacctcctct ctagaaaatc atgaccaaat gtctgtgaac tgcagaacgt
     8701 tgctcagcga gtcccctggt aagctccacg ttgaagtttc agcagatctt acagcaatta
     8761 atggtctttc ttacaatcaa aatctcgcca atggcagtta tgatttagct aacagagact
     8821 tttgccaagg aaatcagcta aattactaca agcaggaaat acccgtgcaa ccaactacct
     8881 catattccat tcagaattta tacagttacg agaaccagcc ccagcccagc gatgaatgta
     8941 ctctcctgag taataaatac cttgatggaa atgctaacaa atctacctca gatggaagtc
     9001 ctgtgatggc cgtaatgcct ggtacgacag acactattca agtgctcaag ggcaggatgg
     9061 attctgagca gagcccttct attgggtact cctcaaggac tcttggcccc aatcctggac
     9121 ttattcttca ggctttgact ctgtcaaacg ctagtgatgg atttaacctg gagcggcttg
     9181 aaatgcttgg cgactccttt ttaaagcatg ccatcaccac atatctattt tgcacttacc
     9241 ctgatgcgca tgagggccgc ctttcatata tgagaagcaa aaaggtcagc aactgtaatc
     9301 tgtatcgcct tggaaaaaag aagggactac ccagccgcat ggtggtgtca atatttgatc
     9361 cccctgtgaa ttggcttcct cctggttatg tagtaaatca agacaaaagc aacacagata
     9421 aatgggaaaa agatgaaatg acaaaagact gcatgctggc gaatggcaaa ctggatgagg
     9481 attacgagga ggaggatgag gaggaggaga gcctgatgtg gagggctccg aaggaagagg
     9541 ctgactatga agatgatttc ctggagtatg atcaggaaca tatcagattt atagataata
     9601 tgttaatggg gtcaggagct tttgtaaaga aaatctctct ttctcctttt tcaaccactg
     9661 attctgcata tgaatggaaa atgcccaaaa aatcctcctt aggtagtatg ccattttcat
     9721 cagattttga ggattttgac tacagctctt gggatgcaat gtgctatctg gatcctagca
     9781 aagctgttga agaagatgac tttgtggtgg ggttctggaa tccatcagaa gaaaactgtg
     9841 gtgttgacac gggaaagcag tccatttctt acgacttgca cactgagcag tgtattgctg
     9901 acaaaagcat agcggactgt gtggaagccc tgctgggctg ctatttaacc agctgtgggg
     9961 agagggctgc tcagcttttc ctctgttcac tggggctgaa ggtgctcccg gtaattaaaa
    10021 ggactgatcg ggaaaaggcc ctgtgcccta ctcgggagaa tttcaacagc caacaaaaga
    10081 acctttcagt gagctgtgct gctgcttctg tggccagttc acgctcttct gtattgaaag
    10141 actcggaata tggttgtttg aagattccac caagatgtat gtttgatcat ccagatgcag
    10201 ataaaacact gaatcacctt atatcggggt ttgaaaattt tgaaaagaaa atcaactaca
    10261 gattcaagaa taaggcttac cttctccagg cttttacaca tgcctcctac cactacaata
    10321 ctatcactga ttgttaccag cgcttagaat tcctgggaga tgcgattttg gactacctca
    10381 taaccaagca cctttatgaa gacccgcggc agcactcccc gggggtcctg acagacctgc
    10441 ggtctgccct ggtcaacaac accatctttg catcgctggc tgtaaagtac gactaccaca
    10501 agtacttcaa agctgtctct cctgagctct tccatgtcat tgatgacttt gtgcagtttc
    10561 agcttgagaa gaatgaaatg caaggaatgg attctgagct taggagatct gaggaggatg
    10621 aagagaaaga agaggatatt gaagttccaa aggccatggg ggatattttt gagtcgcttg
    10681 ctggtgccat ttacatggat agtgggatgt cactggagac agtctggcag gtgtactatc
    10741 ccatgatgcg gccactaata gaaaagtttt ctgcaaatgt accccgttcc cctgtgcgag
    10801 aattgcttga aatggaacca gaaactgcca aatttagccc ggctgagaga acttacgacg
    10861 ggaaggtcag agtcactgtg gaagtagtag gaaaggggaa atttaaaggt gttggtcgaa
    10921 gttacaggat tgccaaatct gcagcagcaa gaagagccct ccgaagcctc aaagctaatc
    10981 aacctcaggt tcccaatagc tattattaga cgcgttaggg atccagacca cctcccctgc
    11041 gagctaagct ggacagccaa tgacgggtaa gagagtgaca tttttcacta acctaagaca
    11101 ggagggccgt cagagctact gcctaatcca aagacgggta aaagtgataa aaatgtatca
    11161 ctccaaccta agacaggcgc agcttccgag ggatttgaga tccagacatg ataagataca
    11221 ttgatgagtt tggacaaacc aaaactagaa tgcagtgaaa aaaatgcctt atttgtgaaa
    11281 tttgtgatgc tattgcctta tttgtaacca ttataagctg caataaacaa gttaacaaca
    11341 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
    11401 agtaaaacct ctacaaatgt ggtatggctg attatgatct gaagttccta tactatttga
    11461 agaataggaa cttcggaata ggaacttcgg atctcatatg gccatatggg ggcgcctaga
    11521 gaaggagtga gggctggata aagggaggat cgaggcgggg tcgaacgagg aggttcaagg
    11581 gggagagacg gggcggatgg aggaagagga ggcggaggct tagggtgtac aaagggcttg
    11641 acccagggag gggggtcaaa agccaaggct tcccaggtca cgatgtaggg gacctggtct
    11701 gggtgtccat gcgggccagg tgaaaagacc ttgatcttaa cctgggtgat gaggtctcgg
    11761 ttaaaggtgc cgtctcgcgg ccatccgacg ttaaaggttg gccattctgc agagcagaag
    11821 gtaacccaac gtctcttctt gacatctacc gactggttgt gagcgatccg ctcgacatct
    11881 ttccagtgac ctaaggtcaa acttaaggga gtggtaacag gctagcccgg gcccatattt
    11941 tcagacaaat acagaaacac agtcagacag agacaacaca gaacgatgct gcagcagaca
    12001 agacgcgcgg cgcggcttcg gtcccaaacc gaaagcaaaa attcagacgg aggcgggaac
    12061 tgttttaggt tctcgtctcc taccagaacc acatatccct cctctaaggg gggtgcacca
    12121 aagagtccta aacgatcggg attttaggac tcaggtcggg ccacaaaaac ggcccccgaa
    12181 gtccctggga cgtctcccag ggttgcggcc gggtgttccg aactcgtcag ttccaccacg
    12241 ggtccgccag atacagagct agttagctaa ctagtaccga cgcaggcgca taaaatcagt
    12301 catagacact agacaatcgg acagacacag ataagttgct ggccagctta cctcccggtg
    12361 gtgggtcggt ggtccctggg caggggtctc ccgatcccgg acgagccccc aaatgaaaga
    12421 cccccgctga cgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac
    12481 aagtcggatg caactgcaag agggtttatt ggatacacgg gtacccgggc gactcagtca
    12541 atcggaggac tggcgcgccg agtgaggggt tgtgggctct tttattgagc tcggggagca
    12601 gaagcgcgcg aacagaagcg agaagcgaac tgattggtta gttcaaataa ggcacagggt
    12661 catttcaggt ccttggggca ccctggaaac atctgatggt tctctagaaa ctgctgaggg
    12721 ctggaccgca tctggggacc atctgttctt ggccctgagc cggggcagga actgcttacc
    12781 acagatatcc tgtttggccc atattcagct gttccatctg ttcttggccc tgagccgggg
    12841 caggaactgc ttaccacaga tatcctgttt ggcccatatt cagctgttcc atctgttcct
    12901 gaccttgatc tgaacttttc tattctcagt tatgtatttt tccatgcctt gcaaaatggc
    12961 gttacttaag ctagcttgcc aaacctacag gtggggtctt tcacatgtat atgtcaaaaa
    13021 taaaaatcaa ctaattgact agtaattaat atgactggca taatgggaaa ttgatcctga
    13081 cagatgcaaa ctggcttctc agcagcgcat ttatgttgtc aactgaggaa ggaaacgtta
    13141 atgacagaaa ctctaagtaa tttccacgtt tatctatttt tatttatact agctttggta
    13201 acaggaatat tgcagcattc atgcacattg aaacccttat gaaataaaaa catctgtgca
    13261 tttaaaatgg aattaacatt ttaaatgtta aaaaaagctg gcttagcttc cccccgcccc
    13321 ctagggcata gaacaagtca aatgctttat atatttgagt ttgggatgta ttaggaaact
    13381 cctaagagca aagctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    13441 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    13501 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    13561 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    13621 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    13681 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    13741 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    13801 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    13861 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    13921 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    13981 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    14041 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    14101 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    14161 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    14221 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    14281 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    14341 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    14401 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    14461 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    14521 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    14581 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    14641 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    14701 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    14761 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    14821 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    14881 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    14941 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    15001 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    15061 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    15121 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    15181 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    15241 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    15301 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    15361 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    15421 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    15481 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    15541 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    15601 tataaaaata ggcgtatcac gaggcccttt cgtc
//
