LOCUS       sequence-18230-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH
  TITLE     Crystal structure of a PhoU protein homologue: a new class of 
            metalloprotein containing multinuclear iron clusters.
  JOURNAL   J Biol Chem. 2005 Apr 22. 280(16):15960-6.
  PUBMED    15716271
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Apr 22. 280(16):15960-6."
COMMENT     Sequence Label: pLIC.B3.TM1734
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..107
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatagtggg cagcagacat catcatcatc
       61 atcacgatta cgatatccca acgaccgaaa acctttactt ccagggcggc ggtggtggcg
      121 gcatgaatag actcttaaac gaaaaggtgg aagaattcaa gaaaggcgtt ctcaaagcgg
      181 gatggttcat agaaaagatg ttcagaaact ccatatcctc acttgtcgaa agaaacgagt
      241 ccctcgccag ggaagtgatc gcagatgaag aggttgtcga tcagatggaa gtggagattc
      301 aggaaaaagc aatggaggtg ctcggcctct tttctcccat aggaaagccc cttctcacag
      361 taacagcggg tataagggtg gcagagctca ttgaaaatat agccgacaaa tgccatgaca
      421 tcgcaaagaa cgtccttgaa ctcatggaag aacctccctt gaaacctctc gaagacattc
      481 cagccatggc gaatcaaact tcagaaatgt tgaagttcgc tctcaggatg ttcgccgatg
      541 tgaacgtaga aaagtctttc gaagtttgca ggatggattc gaaggttgat gacctgtacg
      601 agaaggtgcg tgaagaactc ttgctctaca tgatggagtc acccaaatat gtgaaaaggg
      661 ctcttctcct cttagaaata gccggcaaca tagagatcat agccgattac gccacaacca
      721 tcgtggaagt ctccgt
//
