LOCUS       sequence-182302-       15403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 15403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C
FEATURES             Location/Qualifiers
     source          1..15403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(69..86)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(69..85)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(78..100)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(294..313)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     413..435
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(473..491)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        559..663
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(664..732,733..1524)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(882..901)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1695..2283
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2184..2203
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2437..2454
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2571..2592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2607..2612,2613..2630,2631..2637)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2645..2661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2650..2672
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2669..2685
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2669..2685
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             3215..3684
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3749..4106
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     4517..4539
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4555..4571
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    4624..4657
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     polyA_signal    4765..4899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4802..4821)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7873..7890)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(10998..11027)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        complement(11125..11162)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(11179..11197,11198..11220,11221..11239,11240..11262,
                     11263..11281,11282..11304,11305..11323,11324..11346,
                     11347..11365,11366..11388,11389..11407,11408..11430,
                     11431..11449)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        11464..11502
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             11614..11637
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      13837..13846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    14528..14575
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 caacgctccg gctcaggtgt caggttggtt tttgagacag agtctttcac ttagcttgga
       61 attaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      121 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      181 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
      241 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
      301 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      361 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
      421 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
      481 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
      541 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
      601 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
      661 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
      721 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
      781 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
      841 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
      901 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
      961 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1021 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1081 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1141 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1201 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1261 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1321 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1381 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     1441 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     1501 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     1561 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     1621 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     1681 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     1741 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     1801 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     1861 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     1921 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     1981 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2041 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2101 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2161 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2221 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2281 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2341 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     2401 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     2461 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     2521 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     2581 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     2641 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     2701 ctagctttgc tcttaggagt ttcctaatac atcccaaact caaatatata aagcatttga
     2761 cttgttctat gccctagggg gcggggggaa gctaagccag ctttttttaa catttaaaat
     2821 gttaattcca ttttaaatgc acagatgttt ttatttcata agggtttcaa tgtgcatgaa
     2881 tgctgcaata ttcctgttac caaagctagt ataaataaaa atagataaac gtggaaatta
     2941 cttagagttt ctgtcattaa cgtttccttc ctcagttgac aacataaatg cgctgctgag
     3001 aagccagttt gcatctgtca ggatcaattt cccattatgc cagtcatatt aattactagt
     3061 caattagttg atttttattt ttgacatata catgtgaaag accccacctg taggtttggc
     3121 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagaa
     3181 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct
     3241 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg
     3301 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     3361 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     3421 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     3481 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     3541 cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     3601 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     3661 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     3721 agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg tgtctgtccg
     3781 attgtctagt gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc
     3841 tctgtatctg gcggacccgt ggtggaactg acgagttcgg aacacccggc cgcaaccctg
     3901 ggagacgtcc cagggacttc gggggccgtt tttgtggccc gacctgagtc ctaaaatccc
     3961 gatcgtttag gactctttgg tgcacccccc ttagaggagg gatatgtggt tctggtagga
     4021 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttgggaccga
     4081 agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt gttgtctctg tctgactgtg
     4141 tttctgtatt tgtctgaaaa tatgggcccg ggctagcctg ttaccactcc cttaagtttg
     4201 accttaggtc actggaaaga tgtcgagcgg atcgctcaca accagtcggt agatgtcaag
     4261 aagagacgtt gggttacctt ctgctctgca gaatggccaa cctttaacgt cggatggccg
     4321 cgagacggca cctttaaccg agacctcatc acccaggtta agatcaaggt cttttcacct
     4381 ggcccgcatg gacacccaga ccaggtcccc tacatcgtga cctgggaagc cttggctttt
     4441 gacccccctc cctgggtcaa gccctttgta caccctaagc ctccgcctcc tcttcctcca
     4501 tccgccccgt ctctccccct tgaacctcct cgttcgaccc cgcctcgatc ctccctttat
     4561 ccagccctca ctccttctct aggcgccccc atatggccat atgagatccg aagttcctat
     4621 tccgaagttc ctattcttca aatagtatag gaacttcaga tcataatcag ccataccaca
     4681 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     4741 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     4801 ggcaatagca tcacaaattt cacaaataag gcattttttt cactgcattc tagttttggt
     4861 ttgtccaaac tcatcaatgt atcttatcat gtctggatct caaatccctc ggaagctgcg
     4921 cctgtcttag gttggagtga tacattttta tcacttttac ccgtctttgg attaggcagt
     4981 agctctgacg gccctcctgt cttaggttag tgaaaaatgt cactctctta cccgtcattg
     5041 gctgtccagc ttagctcgca ggggaggtgg tctggatccc taacgcgtct aataatttac
     5101 agggactccc cgctgagcag gtccccaggc agcagggtgg ttagcggcgt ggggctgcct
     5161 atcaccctgc tgtcgtcggc gctgggcggg ccccacaggc tgctggagta ctggggcacc
     5221 ccgccccaca gcagctcact gctccccttg ccacccaggc acggggcgtt ccagtggcca
     5281 gcgtcgctgc gtaccaggcc atgggagctg cccgctgcgc tgagccgcgg ctggctggac
     5341 gcgctgctgg actgccagct ggaagtgggt ggcagcgcct ggccttgggc taagaagcga
     5401 ttcacttctt cttcaccagc gaactcggcc aggatggtag tgtttcccag gacgcacatg
     5461 tgcagagact tctgggcctt ggcagcctcc tccttggagc tgtaccggac cacagcattg
     5521 ccttgagtca gattcaggtg gaatgtgata agaggcccat gttgcaaaca caatgtccgc
     5581 agtgtagaac catcaatctg gggagtgagg tttcgaagaa cgagccagct gctggttctt
     5641 cctgaggtgt cggtgctcca ggcagaaccc gaggagtagg agctggtcca gccgaggggg
     5701 ctggcacccc aggtggagga gggcttggga ttggttaacc ctggaggtgg cctcgtgggt
     5761 gcagtactgt ttctgggcac cttccatagt tcatgagaca gagaggcttg cgtgtgggag
     5821 gtagggccag aggaccacgt cgatttgatg tctgacagtt tacctccact cttgaggagg
     5881 tagcggttga catcctggat ggtggtgttg atggtaggcc cagtggggac actgccagga
     5941 gtgacgtcag ggtcattctc agggtcaata ttctgcagtc ctttccatgg aactcccgga
     6001 tggaattctg ggggccagtt gatgctagag ccatttgaga ttttatcact gtcagatttg
     6061 gcacgtgacc agctatgggg aacagctacg ggaggactgg ctggtgactc actgttctgg
     6121 attaaatcgt accggccata ggagtcatca atggaggact tacctggagg cccaatgctt
     6181 agacctccag ggatagcacc atgcttgcta gggttctgct ccagggggga agcggcactg
     6241 ggcaagttat ccatggagtt ggggtgcgtc cactggggga ggcgtgactg ggactgggat
     6301 gggtccttca ccgacaagcc accggtcatg tccatgctgt tgacattcat gtttgggttc
     6361 agtccagcga gagggtaagg agcaaaggtg ttgggtgaag actgctgctc tttggtctgc
     6421 aggtcaggta ggccgggagt ctgtgggtgc gaggggaagc tgtccatggc cgatttgcct
     6481 gcagaggggt gcagagacag gtgcggcggg ggcggtggcg gtggtggctg cttcacgagc
     6541 agggcctggg ccagctggcg ctggtgctgc tggatctgct gctgcagatt agtgattgtg
     6601 cgcgcaactt gctgctcctg ttgtctcatg gatccggaca cattacgctg ggcctgtaac
     6661 atctgctgct ggatttgtaa acgttggtat gccagctgca gctgatagag ctggttcaac
     6721 atcgtcatat gttgaggatt aattggcgag gttaatagtg cagggttgag accaatgttt
     6781 tttgctgcaa actgcaaaag ctgtgcttga acctgagggg atagaaactg aggcacttga
     6841 gcacggagac tgggctggga agagttaaga ggctgcactg gtggctgtgg cggctgctgc
     6901 atggtcctgg cttgtgctgc tccactattg ccaaacatac ccagattgcc actcggtatc
     6961 tgtctagaag aattcaagtt ttgcatgccc agcccggagg caatcccacc cagggcctga
     7021 ctggggagtg cactgtgtga aagggggagc ttcaggcttg gggaaggcaa gaacggtgaa
     7081 ggcgtgggct cttccacgag gccgccatcc ttgtcaagaa aggtggggcg gtccacggaa
     7141 gactctttgg agattggcgg gcggcagccg aggggcttgg cggccattcc attatggtcg
     7201 gtcactccca gcccacgctt gtccacgtcc accttctttt ccagcagagc gctcatggcc
     7261 tgatcaagat tcatattgtt actcttcaag gcctcctcag ctggctctct cgggaagccc
     7321 atgtctgtga gttgtttgat cagccggctc atgatccagg cctcatcctg cttgccagac
     7381 gtcttcatgc ccttttgaag tccttttttg ggggcgttcc cccaggagct gcaggaggag
     7441 gtgctttctt gggaagcagc attattccac acgtcccctt cttcatcctc ccactggctg
     7501 gtactgaggt tcatttcatc cccaccactg ccccagcctt cttgcataga ttttgaagct
     7561 ggtttgcaca gggctgaggc agcaacaggt gcgccagctc ttccaaatgc caccggcggc
     7621 tcggcagggt gatctcccca cccgctgcca ctgctgggtg gcttccccca tgctgctgtg
     7681 ccattatcca ccagggtaga aggggaggat ggttctcccc aagagttttc agtctttgag
     7741 tgaacattag gcatttctcc ccagcctgat gaaactttcc tacctggacc aagcaacggt
     7801 gatcgattca gctgagtacc agcctggtgc gggggcggcg tctcgaccct gtgtgtggtg
     7861 ttgctcgtgg tagtggtggt ggtggtggtg gtattggtcg tggtactgct ctggatgacc
     7921 gggttgtttc tatcccacat gtttacagtt ttattgttat agttgcttgg gtccccccaa
     7981 gctgaggtac catcatcaat ttccattttg cggcgaatgg acggtggaga gggttcttcc
     8041 cagccagttg cttcactgct gtccttctgt ttgaccggta ccggcccccc gatccaccct
     8101 gttcctgtct gtttcacaga agcagctcct ccccagttac tcacattgtt gtcttggggt
     8161 ttgctagccc agttctgttg ggggccaggt tttaaagtct ccccccagtt tgtacctgga
     8221 ttggcctttg tatttgtgct gttgccccag ccactgttcc cagatctcgt ggtgtcattc
     8281 caaccagacc ctgacctatt actgtcagca tcccatccag atccattttt tttcccatcc
     8341 cccaagtgtc caaggacaga cgatgaatct gcccaatctc cccctcctgc actccaggct
     8401 ggatttgatc tttgtccatc tccccagtgt tgggatttgg gctttggagg ctcaccccag
     8461 gtgggtgact tgtcctcctg atttgcggcc ccactccaag catggccact cttggcagca
     8521 gcagcagtac taacagcagt agagcttatc gagtcccccc aaacccccgg gccacttggc
     8581 gctttgttgt tgctgtctcc ccaacctgtg tttgctggca cagcggcagg tggcgcgttg
     8641 acccacccag atactgaaga ggtatttgta ctgttcatga tggacccatc gttcttcccc
     8701 cctgagtttg aagcctgagt agctgcacaa ccccaggcct ctgtcccatt gtcatttttc
     8761 ctttcagacc taggggattc ttcaaattcc caggcagtgt tttgctttac aggagtctgt
     8821 ccccaaccag tattagacag aactcttggg tcaagatcat tccttggcaa ctggatgtgc
     8881 ccttggtcta taatcccttt atctcgcctt cggccttctc ccgttccttc cctcccagtg
     8941 cttccttcat tgtggttgcc agaaccatca ctacttcctt cacttgctgt agttccagaa
     9001 gatgccgctt tggcccagga gttcatgtgt tcaccaccag gctgtacggt agggttctgg
     9061 ctggtgacac taggactctc ccaccctgtt gatcctttcc cattgatgtc actgttggaa
     9121 tgctggtttg ggggctttcc ccattcccca ttcacaccgt tagaggtgct tcggctgggg
     9181 tggccccaag ctcctgaatg gatattacca acgccattgt tgccactgcc ccttccccag
     9241 gcgagtattc caggaccagc aggaggtcct gaatcccaag catttcctcc acttccttcc
     9301 ttttgcacag cactgctgga tccattttgt ttagcactta atgctgagtt cacagtgtct
     9361 ccattcccct gactgaaccc agaattgcta tttcctgtgg cagggttacc tggggacagt
     9421 ccccacacag aattgcttat tccttcagca ctgcccccac tgacttgaga aactgatgat
     9481 ccattagcac caggaaggcc ttgcaggtgg ggcgggatga tggcccccat accaacagcc
     9541 atgccccagt ttggcagtcc atttgtctgc attgcattga tagggttggg tgaagagttc
     9601 atggggttag tgttatttgg tccatcagtg ttaaggttct gaggttgtgc gctgaaagac
     9661 acattctgag aagtggacgt ttgtggttct gtgctctctt gtggcagcaa gtttccccaa
     9721 gcacctaaag tgcctgttgg gttcccgttc tggttgccca tattttgacc tatggcactg
     9781 actggactgc aaatactgga agggttgcct gtcgccacgg ttccttcatg tccaagtaca
     9841 ggccaggcag ctgggttggc attaggatta aggttaagat taatgccagc attggagttg
     9901 gaagcacccc agcaattctg acttctccca tctccaatca tagtgtccat ttttccatcc
     9961 ccaccactga cagagcagtg agccaggcca gagctggagc ctgtggctac accccacact
    10021 ctggccgcac ttccattact gttcgctccc cctgctccaa gggcactctg attagaaggg
    10081 ctttggacga gtgcgccatt ggtgccatta ttgccgtttg tcttcttggt atgtccagtg
    10141 aagttgccct gggcactccc tgtagccatc tcaatgcccc gggaggcgta ggagatctgg
    10201 ccgccgccgg aggagccgcc gccatgtttc tcctgctcga acagctccag agcggctctc
    10261 agctctctga tcagcatggc ggccagggtg ttccggtcca ggttgatgcc agcctcctgc
    10321 agggtgatcc agccctggtt caccacgctc tcctccactc ttctcatggc catgttgatt
    10381 ccggctccga tcacgatctg agcggcatcg cctgtcttgc ctgtcagctc caccaggatg
    10441 ccagccagct tgcggtcctg gaggtacagg tcattaggcc acttcactct caccttgtcg
    10501 gctcccagct ttctcagcac ctcggccatc acgattccga tcaccagact cagtccgatg
    10561 gcagcagcag gtccctgctc cagtctccag aacatggaca ggtacaggtt ggctccgaat
    10621 gggctgaacc acttccgtcc tctgcctcct ctgccagcct gctggtactc ggcgatgcag
    10681 gcgtcgccgg acttcagctc tccgattctg tccagcaggt actgattggt ggagtcgatc
    10741 acaggcagca cggccacgct tccgccatcc agctgtccca ggatctgctt ggcgttcagc
    10801 agctggatag gctcaggcag gctgtagccc ttgccaggca cggtgaacac gtccactccc
    10861 cagtctctca gtgtctggat gtgcttgttg atggcggctc tgctcattcc cagggtctct
    10921 cccagctgct cgccagagtg gaactcgccg ttggccagca gggcgatcag cttcaggggc
    10981 acggtgttgt ccttgtcgag atcctcttct gagatgagtt tttgttccat ctcgaccgcg
    11041 gaggctggat cggtcccggt gtcttctatg gaggtcaaaa cagcgtggat ggcgtctcca
    11101 ggcgatctga cggttcacta aacgagctct gcttatatag gcctcccacc gtacacgcct
    11161 acaagcttct ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt
    11221 tctctatcac tgatagggag tggtaaactc gactttcact tttctctatc actgataggg
    11281 agtggtaaac tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt
    11341 cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg
    11401 atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc
    11461 gagggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
    11521 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
    11581 agcctccgcg gaattcggcc ggcctcaatt atggactaca aggacgacga tgacaagatt
    11641 aacggactgg ctccttacct gagcagatgg gagaagctgg acaacttcat caacagacct
    11701 gtgaagctga tcatcggcga caaggaaatc ttcggcatct ccagaggaat cgacaagcag
    11761 ggagctctgc tgctggagca ggacggaatc atcaagccct ggatgggcgg agaaatctcc
    11821 ctgagaagcg cagagaagct cgaggcgaac cagatctcct acgcctcccg gggcggcggc
    11881 tcctccggcg gcggcgtttg tttaaacatg atgtactcgg gagccggccc cgcacttgca
    11941 cctcctgcgc cgccgccccc catccaagga tatgccttca agcctccacc tagacccgac
    12001 tttgggacct ccgggagaac aatcaaatta caggccaatt tcttcgaaat ggacatcccc
    12061 aaaattgaca tctatcatta tgaattggat atcaagccag agaagtgccc gaggagagtt
    12121 aacagggaaa tcgtggaaca catggtccag cactttaaaa cacagatctt tggggatcgg
    12181 aagcccgtgt ttgacggcag gaagaatcta tacacagcca tgccccttcc gattgggagg
    12241 gacaaggtgg agctggaggt cacgctgcca ggagaaggca aggatcgcat cttcaaggtg
    12301 tccatcaagt gggtgtcctg cgtgagcttg caggcgttac acgatgcact ttcagggcgg
    12361 ctgcccagcg tcccttttga gacgatccag gccctggacg tggtcatgag gcacttgcca
    12421 tccatgaggt acacccccgt gggccgctcc ttcttcaccg cgtccgaagg ctgctctaac
    12481 cctcttggcg ggggccgaga agtgtggttt ggcttccatc agtccgtccg gccttctctc
    12541 tggaaaatga tgctgaatat tgatgtgtca gcaacagcgt tttacaaggc acagccagta
    12601 atcgagtttg tttgtgaagt tttggatttt aaaagtattg aagaacaaca aaaacctctg
    12661 acagattccc aaagggtaaa gtttaccaaa gaaattaaag gtctaaaggt ggagataacg
    12721 cactgtgggc agatgaagag gaagtaccgt gtctgcaatg tgacccggcg gcccgccagt
    12781 caccaaacat tcccgctgca gcaggagagc gggcagacgg tggagtgcac ggtggcccag
    12841 tatttcaagg acaggcacaa gttggttctg cgctaccccc acctcccatg tttacaagtc
    12901 ggacaggagc agaaacacac ctaccttccc ctggaggtct gtaacattgt ggcaggacaa
    12961 agatgtatta aaaaattaac ggacaatcag acctcaacca tgatcagagc gactgctagg
    13021 tcggcgcccg atcggcaaga agagattagc aaattgatgc gaagtgcaag tttcaacaca
    13081 gatccatacg tccgtgaatt tggaatcatg gtcaaagatg agatgacaga cgtgactggg
    13141 cgggtgctgc agccgccctc catcctctac gggggcagga ataaagctat tgcgacccct
    13201 gtccagggcg tctgggacat gcggaacaag cagttccaca cgggcatcga gatcaaggtg
    13261 tgggccattg cgtgcttcgc cccccagcgc cagtgcacgg aagtccatct gaagtccttc
    13321 acagagcagc tcagaaagat ctcgagagac gccggcatgc ccatccaggg ccagccgtgc
    13381 ttctgcaaat acgcgcaggg ggcggacagc gtggagccca tgttccggca cctgaagaac
    13441 acgtatgcgg gcctgcagct ggtggtggtc atcctgcccg gcaagacgcc cgtgtacgcc
    13501 gaggtcaagc gcgtgggaga cacggtgctg gggatggcca cgcagtgcgt gcagatgaag
    13561 aacgtgcaga ggaccacgcc acagaccctg tccaacctct gcctgaagat caacgtcaag
    13621 ctgggaggcg tgaacaacat cctgctgccc cagggcaggc cgccggtgtt ccagcagccc
    13681 gtcatctttc tgggagcaga cgtcactcac ccccccgccg gggatgggaa gaagccctcc
    13741 attgccgccg tggtgggcag catggacgcc caccccaatc gctactgcgc caccgtgcgc
    13801 gtgcagcagc accggcagga gatcatacaa gacctggccg ccatggtccg cgagctcctc
    13861 atccagttct acaagtccac gcgcttcaag cccacccgca tcatcttcta ccgcgacggt
    13921 gtctctgaag gccagttcca gcaggttctc caccacgagt tgctggccat ccgtgaggcc
    13981 tgtatcaagc tagaaaaaga ctaccagccc gggatcacct tcatcgtggt gcagaagagg
    14041 caccacaccc ggctcttctg cactgacaag aacgagcggg ttgggaaaag tggaaacatt
    14101 ccagcaggca cgactgtgga cacgaaaatc acccacccca ccgagttcga cttctacctg
    14161 tgtagtcacg ctggcatcca ggggacaagc aggccttcgc actatcacgt cctctgggac
    14221 gacaatcgtt tctcctctga tgagctgcag atcctaacct accagctgtg tcacacctac
    14281 gtgcgctgca cacgctccgt gtccatccca gcgccagcat actacgctca cctggtggcc
    14341 ttccgggcca ggtaccacct ggtggataag gaacatgaca gtgctgaagg aagccatacc
    14401 tctgggcaga gtaacgggcg agaccaccaa gcactggcca aggcggtcca ggttcaccaa
    14461 gacactctgc gcaccatgta ctttgcttga ttaattaaat agcgattaat aacgaggatc
    14521 tggccgcgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagatc
    14581 cggattagtc caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca
    14641 acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt
    14701 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctcg
    14761 acttctgttc gcgcgcttct gctccccgag ctcgagccca caacccctca ctcggggcgc
    14821 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
    14881 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
    14941 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
    15001 gtatttacat taaatggcca tagtacttaa agttacattg gcttccttga aataaacatg
    15061 gagtattcag aatgtgtcat aaatatttct aattttaaga tagtatctcc attggctttc
    15121 tactttttct tttatttttt tttgtcctct gtcttccatt tgttgttgtt gttgtttgtt
    15181 tgtttgtttg ttggttggtt ggttaatttt tttttaaaga tcctacacta tagttcaagc
    15241 tagactatta gctactctgt aacccagggt gaccttgaag tcatgggtag cctgctgttt
    15301 tagccttccc acatctaaga ttacaggtat gagctatcat ttttggtata ttgattgatt
    15361 gattgattga tgtgtgtgtg tgtgattgtg tttgtgtgtg tga
//
