LOCUS       sequence-182304-       11041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split-BioID plasmid: Expresses N-terminally tagged 
            CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Schopp IM, Amaya Ramirez CC, Debeljak J, Kreibich E, Skribbe M, Wild
            K, Bethune J
  TITLE     Split-BioID a conditional proteomics approach to monitor the 
            composition of spatiotemporally defined protein complexes.
  JOURNAL   Nat Commun. 2017 Jun 6;8:15690. doi: 10.1038/ncomms15690.
  PUBMED    28585547
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-6"; volume: "8:15690. doi"; pages: " 
            10.1038/ncomms15690"
COMMENT     Sequence Label: pSF3-Flag-Cbir-Ago2_Myc-Nbir-GFP
FEATURES             Location/Qualifiers
     source          1..11041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(828..875)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      1557..1566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3766..3789)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3901..3939)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(3954..3972,3973..3995,3996..4014,4015..4037,4038..4056,
                     4057..4079,4080..4098,4099..4121,4122..4140,4141..4163,
                     4164..4182,4183..4205,4206..4224)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4241..4278
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4376..4405
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(5234..5236,5237..5239,5240..5950)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYDSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5279..5300)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5540..5559)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5887..5908
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6143..6277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6221..6240
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(6385..6418)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(6471..6487)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6503..6525)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6936..7293
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             7358..7827
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8357..8373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8357..8373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8370..8392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8381..8397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8405..8411,8412..8429,8430..8435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8450..8471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8588..8605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8759..9347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8839..8858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9518..10309,10310..10378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10141..10160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10379..10483)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10551..10569
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10607..10629)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10729..10748
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10942..10964
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10956..10973
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10957..10973
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacacacaca aacacaatca cacacacaca catcaatcaa tcaatcaatc aatataccaa
       61 aaatgatagc tcatacctgt aatcttagat gtgggaaggc taaaacagca ggctacccat
      121 gacttcaagg tcaccctggg ttacagagta gctaatagtc tagcttgaac tatagtgtag
      181 gatctttaaa aaaaaattaa ccaaccaacc aacaaacaaa caaacaaaca acaacaacaa
      241 caaatggaag acagaggaca aaaaaaaata aaagaaaaag tagaaagcca atggagatac
      301 tatcttaaaa ttagaaatat ttatgacaca ttctgaatac tccatgttta tttcaaggaa
      361 gccaatgtaa ctttaagtac tatggccatt taatgtaaat acttaagaaa aaaaaccaaa
      421 ttaattttga tacatgctgc atgtgaaaga cccccgctga cgggtagtca atcactcaga
      481 ggagaccctc ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt
      541 ggatacacgg gtacccgggc gactcagtca atcggaggac tggcgccccg agtgaggggt
      601 tgtgggctcg agctcgggga gcagaagcgc gcgaacagaa gtcgagctag cttgccaaac
      661 ctacaggtgg ggtctttcat tccccccttt ttctggagac taaataaaat cttttatttt
      721 atctatggct cgtactctat aggcttcagc tggtgatatt gttgagtcaa aactagagcc
      781 tggaccactg atatcctgtc tttaacaaat tggactaatc cggatctgaa gttcctatac
      841 tttctagaga ataggaactt cggaatagga acttcgcggc cagatcctcg ttattaatcg
      901 ctatttaatt aatcaagcaa agtacatggt gcgcagagtg tcttggtgaa cctggaccgc
      961 cttggccagt gcttggtggt ctcgcccgtt actctgccca gaggtatggc ttccttcagc
     1021 actgtcatgt tccttatcca ccaggtggta cctggcccgg aaggccacca ggtgagcgta
     1081 gtatgctggc gctgggatgg acacggagcg tgtgcagcgc acgtaggtgt gacacagctg
     1141 gtaggttagg atctgcagct catcagagga gaaacgattg tcgtcccaga ggacgtgata
     1201 gtgcgaaggc ctgcttgtcc cctggatgcc agcgtgacta cacaggtaga agtcgaactc
     1261 ggtggggtgg gtgattttcg tgtccacagt cgtgcctgct ggaatgtttc cacttttccc
     1321 aacccgctcg ttcttgtcag tgcagaagag ccgggtgtgg tgcctcttct gcaccacgat
     1381 gaaggtgatc ccgggctggt agtctttttc tagcttgata caggcctcac ggatggccag
     1441 caactcgtgg tggagaacct gctggaactg gccttcagag acaccgtcgc ggtagaagat
     1501 gatgcgggtg ggcttgaagc gcgtggactt gtagaactgg atgaggagct cgcggaccat
     1561 ggcggccagg tcttgtatga tctcctgccg gtgctgctgc acgcgcacgg tggcgcagta
     1621 gcgattgggg tgggcgtcca tgctgcccac cacggcggca atggagggct tcttcccatc
     1681 cccggcgggg gggtgagtga cgtctgctcc cagaaagatg acgggctgct ggaacaccgg
     1741 cggcctgccc tggggcagca ggatgttgtt cacgcctccc agcttgacgt tgatcttcag
     1801 gcagaggttg gacagggtct gtggcgtggt cctctgcacg ttcttcatct gcacgcactg
     1861 cgtggccatc cccagcaccg tgtctcccac gcgcttgacc tcggcgtaca cgggcgtctt
     1921 gccgggcagg atgaccacca ccagctgcag gcccgcatac gtgttcttca ggtgccggaa
     1981 catgggctcc acgctgtccg ccccctgcgc gtatttgcag aagcacggct ggccctggat
     2041 gggcatgccg gcgtctctcg agatctttct gagctgctct gtgaaggact tcagatggac
     2101 ttccgtgcac tggcgctggg gggcgaagca cgcaatggcc cacaccttga tctcgatgcc
     2161 cgtgtggaac tgcttgttcc gcatgtccca gacgccctgg acaggggtcg caatagcttt
     2221 attcctgccc ccgtagagga tggagggcgg ctgcagcacc cgcccagtca cgtctgtcat
     2281 ctcatctttg accatgattc caaattcacg gacgtatgga tctgtgttga aacttgcact
     2341 tcgcatcaat ttgctaatct cttcttgccg atcgggcgcc gacctagcag tcgctctgat
     2401 catggttgag gtctgattgt ccgttaattt tttaatacat ctttgtcctg ccacaatgtt
     2461 acagacctcc aggggaaggt aggtgtgttt ctgctcctgt ccgacttgta aacatgggag
     2521 gtgggggtag cgcagaacca acttgtgcct gtccttgaaa tactgggcca ccgtgcactc
     2581 caccgtctgc ccgctctcct gctgcagcgg gaatgtttgg tgactggcgg gccgccgggt
     2641 cacattgcag acacggtact tcctcttcat ctgcccacag tgcgttatct ccacctttag
     2701 acctttaatt tctttggtaa actttaccct ttgggaatct gtcagaggtt tttgttgttc
     2761 ttcaatactt ttaaaatcca aaacttcaca aacaaactcg attactggct gtgccttgta
     2821 aaacgctgtt gctgacacat caatattcag catcattttc cagagagaag gccggacgga
     2881 ctgatggaag ccaaaccaca cttctcggcc cccgccaaga gggttagagc agccttcgga
     2941 cgcggtgaag aaggagcggc ccacgggggt gtacctcatg gatggcaagt gcctcatgac
     3001 cacgtccagg gcctggatcg tctcaaaagg gacgctgggc agccgccctg aaagtgcatc
     3061 gtgtaacgcc tgcaagctca cgcaggacac ccacttgatg gacaccttga agatgcgatc
     3121 cttgccttct cctggcagcg tgacctccag ctccaccttg tccctcccaa tcggaagggg
     3181 catggctgtg tatagattct tcctgccgtc aaacacgggc ttccgatccc caaagatctg
     3241 tgttttaaag tgctggacca tgtgttccac gatttccctg ttaactctcc tcgggcactt
     3301 ctctggcttg atatccaatt cataatgata gatgtcaatt ttggggatgt ccatttcgaa
     3361 gaaattggcc tgtaatttga ttgttctccc ggaggtccca aagtcgggtc taggtggagg
     3421 cttgaaggca tatccttgga tggggggcgg cggcgcagga ggtgcaagtg cggggccggc
     3481 tcccgagtac atcatgttta aacaaacgcc gccgccggag gagccgccgc cccgggaggc
     3541 gtaggagatc tggttcgcct cgagcttctc tgcgcttctc agggagattt ctccgcccat
     3601 ccagggcttg atgattccgt cctgctccag cagcagagct ccctgcttgt cgattcctct
     3661 ggagatgccg aagatttcct tgtcgccgat gatcagcttc acaggtctgt tgatgaagtt
     3721 gtccagcttc tcccatctgc tcaggtaagg agccagtccg ttaatcttgt catcgtcgtc
     3781 cttgtagtcc ataattgagg ccggccgaat tccgcggagg ctggatcggt cccggtgtct
     3841 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
     3901 agctctgctt atataggcct cccaccgtac acgcctaccc tcgagtttac cactccctat
     3961 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4021 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4081 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4141 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     4201 accactccct atcagtgata gagaaaagtg aaagaagctt gtaggcgtgt acggtgggag
     4261 gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     4321 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggtcg agatggaaca
     4381 aaaactcatc tcagaagagg atctcgacaa ggacaacacc gtgcccctga agctgatcgc
     4441 cctgctggcc aacggcgagt tccactctgg cgagcagctg ggagagaccc tgggaatgag
     4501 cagagccgcc atcaacaagc acatccagac actgagagac tggggagtgg acgtgttcac
     4561 cgtgcctggc aagggctaca gcctgcctga gcctatccag ctgctgaacg ccaagcagat
     4621 cctgggacag ctggatggcg gaagcgtggc cgtgctgcct gtgatcgact ccaccaatca
     4681 gtacctgctg gacagaatcg gagagctgaa gtccggcgac gcctgcatcg ccgagtacca
     4741 gcaggctggc agaggaggca gaggacggaa gtggttcagc ccattcggag ccaacctgta
     4801 cctgtccatg ttctggagac tggagcaggg acctgctgct gccatcggac tgagtctggt
     4861 gatcggaatc gtgatggccg aggtgctgag aaagctggga gccgacaagg tgagagtgaa
     4921 gtggcctaat gacctgtacc tccaggaccg caagctggct ggcatcctgg tggagctgac
     4981 aggcaagaca ggcgatgccg ctcagatcgt gatcggagcc ggaatcaaca tggccatgag
     5041 aagagtggag gagagcgtgg tgaaccaggg ctggatcacc ctgcaggagg ctggcatcaa
     5101 cctggaccgg aacaccctgg ccgccatgct gatcagagag ctgagagccg ctctggagct
     5161 gttcgagcag gagaaacatg gcggcggctc ctccggcggc ggccagatct cctacgcctc
     5221 ccggggcatc gatatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5281 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5341 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5401 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     5461 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5521 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5581 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5641 catcctgggg cacaagctgg agtacaacta cgacagccac aacgtctata tcatggccga
     5701 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     5761 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5821 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     5881 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5941 gctgtacaag ccaacgcgtt agggatccag accacctccc ctgcgagcta agctggacag
     6001 ccaatgacgg gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc
     6061 tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag
     6121 gcgcagcttc cgagggattt gagatccaga catgataaga tacattgatg agtttggaca
     6181 aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc
     6241 cttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     6301 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     6361 atgtggtatg gctgattatg atctgaagtt cctatactat ttgaagaata ggaacttcgg
     6421 aataggaact tcggatctca tatggccata tgggggcgcc tagagaagga gtgagggctg
     6481 gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga gacggggcgg
     6541 atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag ggaggggggt
     6601 caaaagccaa ggcttcccag gtcacgatgt aggggacctg gtctgggtgt ccatgcgggc
     6661 caggtgaaaa gaccttgatc ttaacctggg tgatgaggtc tcggttaaag gtgccgtctc
     6721 gcggccatcc gacgttaaag gttggccatt ctgcagagca gaaggtaacc caacgtctct
     6781 tcttgacatc taccgactgg ttgtgagcga tccgctcgac atctttccag tgacctaagg
     6841 tcaaacttaa gggagtggta acaggctagc ccgggcccat attttcagac aaatacagaa
     6901 acacagtcag acagagacaa cacagaacga tgctgcagca gacaagacgc gcggcgcggc
     6961 ttcggtccca aaccgaaagc aaaaattcag acggaggcgg gaactgtttt aggttctcgt
     7021 ctcctaccag aaccacatat ccctcctcta aggggggtgc accaaagagt cctaaacgat
     7081 cgggatttta ggactcaggt cgggccacaa aaacggcccc cgaagtccct gggacgtctc
     7141 ccagggttgc ggccgggtgt tccgaactcg tcagttccac cacgggtccg ccagatacag
     7201 agctagttag ctaactagta ccgacgcagg cgcataaaat cagtcataga cactagacaa
     7261 tcggacagac acagataagt tgctggccag cttacctccc ggtggtgggt cggtggtccc
     7321 tgggcagggg tctcccgatc ccggacgagc ccccaaatga aagacccccg ctgacgggta
     7381 gtcaatcact cagaggagac cctcccaagg aacagcgaga ccacaagtcg gatgcaactg
     7441 caagagggtt tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc
     7501 gccgagtgag gggttgtggg ctcttttatt gagctcgggg agcagaagcg cgcgaacaga
     7561 agcgagaagc gaactgattg gttagttcaa ataaggcaca gggtcatttc aggtccttgg
     7621 ggcaccctgg aaacatctga tggttctcta gaaactgctg agggctggac cgcatctggg
     7681 gaccatctgt tcttggccct gagccggggc aggaactgct taccacagat atcctgtttg
     7741 gcccatattc agctgttcca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca
     7801 cagatatcct gtttggccca tattcagctg ttccatctgt tcctgacctt gatctgaact
     7861 tttctattct cagttatgta tttttccatg ccttgcaaaa tggcgttact taagctagct
     7921 tgccaaacct acaggtgggg tctttcacat gtatatgtca aaaataaaaa tcaactaatt
     7981 gactagtaat taatatgact ggcataatgg gaaattgatc ctgacagatg caaactggct
     8041 tctcagcagc gcatttatgt tgtcaactga ggaaggaaac gttaatgaca gaaactctaa
     8101 gtaatttcca cgtttatcta tttttattta tactagcttt ggtaacagga atattgcagc
     8161 attcatgcac attgaaaccc ttatgaaata aaaacatctg tgcatttaaa atggaattaa
     8221 cattttaaat gttaaaaaaa gctggcttag cttccccccg ccccctaggg catagaacaa
     8281 gtcaaatgct ttatatattt gagtttggga tgtattagga aactcctaag agcaaagcta
     8341 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8401 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8521 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    10441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    10501 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
    10561 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    10621 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    10681 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
    10741 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
    10801 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
    10861 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
    10921 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattaa
    10981 ttccaagcta agtgaaagac tctgtctcaa aaaccaacct gacacctgag ccggagcgtt
    11041 g
//
