LOCUS       sequence-182306-       14590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lox-STOP-Lox reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14590)
  AUTHORS   Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama-Garren E, Joshi N,
            Hendrickson EA, Feldser D, Yin H, Anderson DG, Jacks T, Weng Z, Xue 
            W
  TITLE     A versatile reporter system for CRISPR-mediated chromosomal 
            rearrangements.
  JOURNAL   Genome Biol. 2015 May 28;16(1):111.
  PUBMED    26018130
REFERENCE   2  (bases 1 to 14590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-05-28"; volume: "16(1)"; pages: "111"
COMMENT     Sequence Label: LSL
FEATURES             Location/Qualifiers
     source          1..14590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     330..352
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     414..433
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     466..482
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    496..529
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        559..1058
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(594..617)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     991..1010
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1072..1875
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1135..1154)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1745..1764
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1885..2346
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     polyA_signal    2494..2628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    2748..2882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3002..3136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3165..3198
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3234..3851,3852..3857,3858..3893,3894..3896,3897..3932,
                     3933..3935,3936..3971,3972..3977)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             3993..4046
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4053..5480
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWETSTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             5496..5555
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5562..7211
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             7218..7265
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             7269..7388
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    7433..7554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7652..7668)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7678..7725)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(8601..8620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8602..8620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8630..8647)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8630..8646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8639..8661)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8788..9243
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8875..8894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9085..9106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9269..9373
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9374..9442,9443..10234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9592..9611)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10405..10993
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10894..10913
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11147..11164
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(11317..11322,11323..11340,11341..11347)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11355..11371
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11360..11382
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11379..11395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11379..11395
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11414..11434
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11416..11434
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    12302..12426
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(12451..12456,12457..12474,12475..12481)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             12535..13194
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(12601..12620)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             13536..13841
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVNVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     13720..13739
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(13882..14006)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        14049..14428
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
       61 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      121 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      181 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      241 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
      301 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
      361 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
      421 gctggttatt gtgctgtctc atcattttgg caaagaattg gccgctctag aactagtgga
      481 tccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtccctcg
      541 acctgcagga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
      601 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
      661 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
      721 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
      781 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
      841 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
      901 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
      961 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     1021 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccaa tatgggatcg
     1081 gccattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     1141 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     1201 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     1261 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     1321 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     1381 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     1441 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     1501 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     1561 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     1621 ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     1681 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     1741 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     1801 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     1861 gacgagttct tctgagggga tccgctgtaa gtctgcagaa attgatgatc tattaaacaa
     1921 taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac
     1981 agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt
     2041 agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata
     2101 gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact
     2161 catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt
     2221 tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc
     2281 agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca
     2341 tcagaagctt gcagatctgc gactctagag gatctgcgac tctagaggat cataatcagc
     2401 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     2461 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     2521 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2581 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg cgactctaga
     2641 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2701 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2761 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2821 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2881 tctgcgactc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     2941 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3001 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3121 ttatcatgtc tggatcccca tcaagctgat ccggaaccct taatataact tcgtataatg
     3181 tatgctatac gaagttatta ggtccctcga cctgcagccc aagctaggcc accatgtcta
     3241 gactggacaa gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg
     3301 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     3361 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     3421 atcataccca cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     3481 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     3541 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     3601 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     3661 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     3721 ccgattctat gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg
     3781 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     3841 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     3901 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     3961 ttgacatgct ccccggggga agtggagcta gggagggcag aggaagtctt ctaacatgcg
     4021 gtgacgtgga ggagaatccc ggccctgcta gaatggtgag caagggcgag gaggtcatca
     4081 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     4141 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     4201 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     4261 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     4321 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     4381 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     4441 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     4501 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     4561 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     4621 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     4681 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
     4741 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
     4801 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
     4861 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     4921 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     4981 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     5041 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     5101 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     5161 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gagacctcca
     5221 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     5281 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     5341 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     5401 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     5461 gcatggacga gctgtacaag ggaagtggat ctagccagtg tactaattat gctctcttga
     5521 aattggctgg agatgttgag agcaacccag gtccctctag gatggaagat gccaaaaaca
     5581 ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc
     5641 acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata
     5701 tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
     5761 tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc
     5821 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     5881 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     5941 tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac
     6001 aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     6061 tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg
     6121 accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg
     6181 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     6241 tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     6301 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     6361 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     6421 tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa
     6481 gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     6541 tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca
     6601 gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc
     6661 ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc
     6721 gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     6781 ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     6841 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     6901 accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg
     6961 ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc
     7021 tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta
     7081 caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga
     7141 ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca
     7201 agatcgccgt gaattctgct tgcaagaact ggttcagtag cttaagccac tttgtgatcc
     7261 accttaacag ccacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
     7321 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
     7381 tcaacgtcta acctagaact agcttatcga tggccgcttc gagcagacat gataagatac
     7441 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     7501 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     7561 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     7621 aagtaaaacc tctacaaatg tggtaaaatc ggatccacta gttctagagc ggccatcgaa
     7681 gttcctatac tttctagaga ataggaactt cggaatagta acttctccat aagcttcagc
     7741 tgctcgagac cggtggatcc gaattcagac cgcggtgggc gggagtcttc tgggcaggct
     7801 taaaggctaa cctggtgtgt gggcgttgtc ctgcagggga attgaacagg tgtaaaattg
     7861 gagggacaag acttcccaca gattttcggt tttgtcggga agttttttaa taggggcaaa
     7921 taaggaaaat gggaggatag gtagtcatct ggggttttat gcagcaaaac tacaggttat
     7981 tattgcttgt gatccgcctc ggagtatttt ccatcgaggt agattaaaga catgctcacc
     8041 cgagttttat actctcctgc ttgagatcct tactacagta tgaaattaca gtgtcgcgag
     8101 ttagactatg taagcagaat tttaatcatt tttaaagagc ccagtacttc atatccattt
     8161 ctcccgctcc ttctgcagcc ttatcaaaag gtattttaga acactcattt tagccccatt
     8221 ttcatttatt atactggctt atccaacccc tagacagagc attggcattt tccctttcct
     8281 gatcttagaa gtctgatgac tcatgaaacc agacagatta gttacataca ccacaaatcg
     8341 aggctgtagc tggggcctca acactgcagt tcttttataa ctccttagta cactttttgt
     8401 tgatcctttg ccttgatcct taattttcag tgtctatcac ctctcccgtc aggtggtgtt
     8461 ccacatttgg gcctattctc agtccaggga gttttacaac aatagatgta ttgagaatcc
     8521 aacctaaagc ttaactttcc actcccatga atgcctctct cctttttctc catttataaa
     8581 ctgagctggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     8641 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8701 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8761 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     8821 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8881 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8941 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     9001 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     9061 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9121 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9181 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     9241 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9301 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9361 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9421 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     9481 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     9541 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9601 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9661 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9721 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9781 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9841 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9901 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9961 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    10021 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10081 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10141 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10201 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    10261 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10321 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10381 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10441 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10501 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10561 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10621 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10681 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10741 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10801 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10861 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10921 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10981 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    11041 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    11101 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11161 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11221 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11281 taatgtgagt tagctcagtc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11341 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    11401 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tctgttttgg
    11461 ttggcgtaag gcgcctgtca gttaacggca gccggagtgc gcagccgccg gcagcctcgc
    11521 tctgcccact gggtggggcg ggaggtaggt ggggtgaggc gagctggacg tgcgggcgcg
    11581 gtcggcctct ggcggggcgg ggggggggag ggagggtcag cgaaagtagc tcgcgcgcga
    11641 gcggccgccc accctcccct tcctctgggg gagtcgtttt acccgccgcc ggccgggcct
    11701 cgtcgtctga ttggctctcg gggcccagaa aactggccct tgccattggc tcgtgttcgt
    11761 gcaagttgag tccatccgcc ggccagcggg ggcggcgagg aggcgctccc aggttccggc
    11821 cctcccctcg gccccgcgcc gcagagtctg gccgcgcgcc cctgcgcaac gtggcaggaa
    11881 gcgcgcgctg ggggcgggga cgggcagtag ggctgagcgg ctgcggggcg ggtgcaagca
    11941 cgtttccgac ttgagttgcc tcaagagggg cgtgctgagc cagacctcca tcgcgcactc
    12001 cggggagtgg agggaaggag cgagggctca gttgggctgt tttggaggca ggaagcactt
    12061 gctctcccaa agtcgctctg agttgttatc agtaagggag ctgcagtgga gtaggcgggg
    12121 agaaggccgc acccttctcc ggagggggga ggggagtgtt gcaatacctt tctgggagtt
    12181 ctctgctgcc tcctggcttc tgaggaccgc cctgggcctg ggagaatccc ttccccctct
    12241 tccctcgtga tctgcaactc cagtctttct cccgggagag tcgaggaatt ctgcagatat
    12301 cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
    12361 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
    12421 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
    12481 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
    12541 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
    12601 gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
    12661 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
    12721 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
    12781 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
    12841 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
    12901 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
    12961 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
    13021 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
    13081 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
    13141 agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc gtaaacgcgt
    13201 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
    13261 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
    13321 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
    13381 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
    13441 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
    13501 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
    13561 aaagagagag ccgttatcgt ctgtttgtga atgtacagag tgatattatt gacacgcccg
    13621 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
    13681 aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
    13741 ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
    13801 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
    13861 acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta
    13921 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
    13981 cgttcagctt tcttgtacaa agtggtgata aacccgctga tcagctcgac gtttaaacct
    14041 cgagattcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
    14101 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
    14161 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
    14221 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
    14281 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
    14341 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
    14401 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
    14461 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
    14521 tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
    14581 ggggcggggc
//
