LOCUS       sequence-182353-       14914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of 
            the Rosa26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14914)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 14914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: pRosa26-4xInsulator (JP6)
FEATURES             Location/Qualifiers
     source          1..14914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2218..2234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(5845..5910,5911..5948)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        5977..6356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6358..6631
                     /label=chicken beta-actin promoter
     misc_feature    9834..14172
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(14222..14238)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14222..14238)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(14270..14289)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14271..14289)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14299..14316)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14299..14315)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14308..14330)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14457..14912
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14544..14563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14754..14775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcacgtgt tcgaaacgcg gtccgccctg cagcaaccgg
     2281 agggggaggg agaagggagc ggaaaagtct ccaccggacg cggccatggc tcgggggggg
     2341 gggggcagcg gaggagcgct tccggccgac gtctcgtcgc tgattggctt cttttcctcc
     2401 cgccgtgtgt gaaaacacaa atggcgtgtt ttggttggcg taaggcgcct gtcagttaac
     2461 ggcagccgga gtgcgcagcc gccggcagcc tcgctctgcc cactgggtgg ggcgggaggt
     2521 aggtggggtg aggcgagctg gacgtgcggg cgcggtcggc ctctggcggg gcgggggagg
     2581 ggagggaggg tcagcgaaag tagctcgcgc gcgagcggcc gcccaccctc cccttcctct
     2641 gggggagtcg ttttacccgc cgccggccgg gcctcgtcgt ctgattggct ctcggggccc
     2701 agaaaactgg cccttgccat tggctcgtgt tcgtgcaagt tgagtccatc cgccggccag
     2761 cgggggcggc gaggaggcgc tcccaggttc cggccctccc ctcggccccg cgccgcagag
     2821 tctggccgcg cgcccctgcg caacgtggca ggaagcgcgc gctgggggcg gggacgggca
     2881 gtagggctga gcggctgcgg ggcgggtgca agcacgtttc cgacttgagt tgcctcaaga
     2941 ggggcgtgct gagccagacc tccatcgcgc actccgggga gtggagggaa ggagcgaggg
     3001 ctcagttggg ctgttttgga ggcaggaagc acttgctctc ccaaagtcgc tctgagttgt
     3061 tatcagtaag ggagctgcag tggagtaggc ggggagaagg ccgcaccctt ctccggaggg
     3121 gggaggggag tgttgcaata cctttctggg agttctctgc tgcctcctgg cttctgagga
     3181 ccgccctggg cacatgatgg acctgtcgac ccccatcctc actgactccg tcctggagta
     3241 ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat
     3301 ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca
     3361 ccgtccctgg aggtggtgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg
     3421 cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga
     3481 tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg
     3541 acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat
     3601 caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc
     3661 catgctgggt tttaacttgt tctttgtcct tctatcctat cttctgatcc ttaaggctgt
     3721 tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc
     3781 atgctgtcct gctcttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca
     3841 aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata ggacaggagg
     3901 gaatggcttt aaactgagac aggggaggtt taggttggat atgaggaggc agtttttccc
     3961 ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg
     4021 caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttggcgacc
     4081 ctgcacatag cagggggttg gaactggatg agcattgtgg gcctttccaa cccaggccat
     4141 tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc
     4201 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     4261 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgtc
     4321 gggccggagc ggagccccgg gcggctcgct gctgccccct agcgggagag ggacgcaatt
     4381 acagccctgg gggctttggg ggggggctct ccccgtgagc tcccgcggac gcccccttcc
     4441 aggaccttcc aggaaagcct gcttcttttc ttcaaccttt ccccccatcc tcactgactc
     4501 cgtcctggag taggatgaga gataatggcc ttacgttgtg ccaggggagg gtcgggctgg
     4561 atttagcaag atttaccttc tccaaagagc ggtgctgcag tggcacagct gcccacggag
     4621 gtgggggggt caccgtccct ggaggtggtg aagaactgtg gggatgtggc actgagggac
     4681 atggccagtg ggcacggtgg gtgggttggg gttggtcttg gggatcttgg agggcttttc
     4741 cagccttcat gatttgacga ttgtatgaac atctacatgg caattctcca gctgcctgtc
     4801 ccagtcctac tgacccagct gtatctctcc aggcaagctc ttccacccct tctgcttgca
     4861 tccagacacc atcaaacatg caggctcaga cacagggacc agcagtgtct gtggcctttt
     4921 tgtgctcctc tccatgctgg gttttaactt gttctttgtc cttctatcct atcttctgat
     4981 ccttaaggct gttctgaacg ctgtgacttg gagagtgtcc cagagccctc aacacctgca
     5041 tgtcccacgt ccatgctgtc ctgctcttcc ttatccccaa gatctgcctc tccgtgatgc
     5101 actgaattgg caaacatgtg tcaccccaga ccaacaatgt cacagcaaac tcccccttga
     5161 taggacagga gggaatggct ttaaactgag acaggggagg tttaggttgg atatgaggag
     5221 gcagtttttc ccccagaggg tggtgacgca ctgaacaggt tgcccaagga ggctgtggat
     5281 gccccatccc tgcaggcatt caaggccagg ctggatgtgg ctctgggcag cctgggctgc
     5341 tggttggcga ccctgcacat agcagggggt tggaactgga tgagcattgt gggcctttcc
     5401 aacccaggcc attctatgat tctgtcattc taaatctctc tttcagccta aagctttttc
     5461 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     5521 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     5581 ggggggagcg tcgggccgga gcggagcccc gggcggctcg ctgctgcccc ctagcgggag
     5641 agggacgcaa ttacagccct gggggctttg ggggggggct ctccccgtga gctcccgcgg
     5701 acgccccctt ccaggacctt ccaggaaagc ctgcttcttt tcttcaacct ttcttaattg
     5761 gccgtggcta gtcgaaactg ggcctgggag aatcccttcc ccctcttccc tcgtgatctg
     5821 caactccagt ctttctagca tctgtagggc gcagtagtcc agggtttcct tgatgatgtc
     5881 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc
     5941 tttccagtgg ttaatgcacc ttccttcctt cgagtcgaca ttgattattg actagttatt
     6001 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     6061 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6121 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6181 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6241 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6301 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     6361 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     6421 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc
     6481 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
     6541 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     6601 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     6661 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     6721 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
     6781 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
     6841 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     6901 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
     6961 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
     7021 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     7081 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
     7141 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     7201 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     7261 ggaggggcgc ggcggccccc ggagcgccgg cggatcgact cttaattaag atatcgtcga
     7321 cccccatcct cactgactcc gtcctggagt aggatgagag ataatggcct tacgttgtgc
     7381 caggggaggg tcgggctgga tttagcaaga tttaccttct ccaaagagcg gtgctgcagt
     7441 ggcacagctg cccacggagg tgggggggtc accgtccctg gaggtggtga agaactgtgg
     7501 ggatgtggca ctgagggaca tggccagtgg gcacggtggg tgggttgggg ttggtcttgg
     7561 ggatcttgga gggcttttcc agccttcatg atttgacgat tgtatgaaca tctacatggc
     7621 aattctccag ctgcctgtcc cagtcctact gacccagctg tatctctcca ggcaagctct
     7681 tccacccctt ctgcttgcat ccagacacca tcaaacatgc aggctcagac acagggacca
     7741 gcagtgtctg tggccttttt gtgctcctct ccatgctggg ttttaacttg ttctttgtcc
     7801 ttctatccta tcttctgatc cttaaggctg ttctgaacgc tgtgacttgg agagtgtccc
     7861 agagccctca acacctgcat gtcccacgtc catgctgtcc tgctcttcct tatccccaag
     7921 atctgcctct ccgtgatgca ctgaattggc aaacatgtgt caccccagac caacaatgtc
     7981 acagcaaact cccccttgat aggacaggag ggaatggctt taaactgaga caggggaggt
     8041 ttaggttgga tatgaggagg cagtttttcc cccagagggt ggtgacgcac tgaacaggtt
     8101 gcccaaggag gctgtggatg ccccatccct gcaggcattc aaggccaggc tggatgtggc
     8161 tctgggcagc ctgggctgct ggttggcgac cctgcacata gcagggggtt ggaactggat
     8221 gagcattgtg ggcctttcca acccaggcca ttctatgatt ctgtcattct aaatctctct
     8281 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     8341 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     8401 gctgccggct cggggatgcg gggggagcgt cgggccggag cggagccccg ggcggctcgc
     8461 tgctgccccc tagcgggaga gggacgcaat tacagccctg ggggctttgg gggggggctc
     8521 tccccgtgag ctcccgcgga cgcccccttc caggaccttc caggaaagcc tgcttctttt
     8581 cttcaacctt tccccccatc ctcactgact ccgtcctgga gtaggatgag agataatggc
     8641 cttacgttgt gccaggggag ggtcgggctg gatttagcaa gatttacctt ctccaaagag
     8701 cggtgctgca gtggcacagc tgcccacgga ggtggggggg tcaccgtccc tggaggtggt
     8761 gaagaactgt ggggatgtgg cactgaggga catggccagt gggcacggtg ggtgggttgg
     8821 ggttggtctt ggggatcttg gagggctttt ccagccttca tgatttgacg attgtatgaa
     8881 catctacatg gcaattctcc agctgcctgt cccagtccta ctgacccagc tgtatctctc
     8941 caggcaagct cttccacccc ttctgcttgc atccagacac catcaaacat gcaggctcag
     9001 acacagggac cagcagtgtc tgtggccttt ttgtgctcct ctccatgctg ggttttaact
     9061 tgttctttgt ccttctatcc tatcttctga tccttaaggc tgttctgaac gctgtgactt
     9121 ggagagtgtc ccagagccct caacacctgc atgtcccacg tccatgctgt cctgctcttc
     9181 cttatcccca agatctgcct ctccgtgatg cactgaattg gcaaacatgt gtcaccccag
     9241 accaacaatg tcacagcaaa ctcccccttg ataggacagg agggaatggc tttaaactga
     9301 gacaggggag gtttaggttg gatatgagga ggcagttttt cccccagagg gtggtgacgc
     9361 actgaacagg ttgcccaagg aggctgtgga tgccccatcc ctgcaggcat tcaaggccag
     9421 gctggatgtg gctctgggca gcctgggctg ctggttggcg accctgcaca tagcaggggg
     9481 ttggaactgg atgagcattg tgggcctttc caacccaggc cattctatga ttctgtcatt
     9541 ctaaatctct ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc
     9601 aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg
     9661 ctgtccccgc acgctgccgg ctcggggatg cggggggagc gtcgggccgg agcggagccc
     9721 cgggcggctc gctgctgccc cctagcggga gagggacgca attacagccc tgggggcttt
     9781 gggggggggc tctccccgtg agcttaatta acggcggcgg agtatcgaat tcgctagaag
     9841 atgggcggga gtcttctggg caggcttaaa ggctaacctg gtgtgtgggc gttgtcctgc
     9901 aggggaattg aacaggtgta aaattggagg gacaagactt cccacagatt ttcggttttg
     9961 tcgggaagtt ttttaatagg ggcaaataag gaaaatggga ggataggtag tcatctgggg
    10021 ttttatgcag caaaactaca ggttattatt gcttgtgatc cgcctcggag tattttccat
    10081 cgaggtagat taaagacatg ctcacccgag ttttatactc tcctgcttga gatccttact
    10141 acagtatgaa attacagtgt cgcgagttag actatgtaag cagaatttta atcattttta
    10201 aagagcccag tacttcatat ccatttctcc cgctccttct gcagccttat caaaaggtat
    10261 tttagaacac tcattttagc cccattttca tttattatac tggcttatcc aacccctaga
    10321 cagagcattg gcattttccc tttcctgatc ttagaagtct gatgactcat gaaaccagac
    10381 agattagtta catacaccac aaatcgaggc tgtagctggg gcctcaacac tgcagttctt
    10441 ttataactcc ttagtacact ttttgttgat cctttgcctt gatccttaat tttcagtgtc
    10501 tatcacctct cccgtcaggt ggtgttccac atttgggcct attctcagtc cagggagttt
    10561 tacaacaata gatgtattga gaatccaacc taaagcttaa ctttccactc ccatgaatgc
    10621 ctctctcctt tttctccatt tataaactga gctattaacc attaatggtt tccaggtgga
    10681 tgtctcctcc cccaatatta cctgatgtat cttacatatt gccaggctga tattttaaga
    10741 cattaaaagg tatatttcat tattgagcca catggtattg attactgctt actaaaattt
    10801 tgtcattgta cacatctgta aaaggtggtt ccttttggaa tgcaaagttc aggtgtttgt
    10861 tgtctttcct gacctaaggt cttgtgagct tgtatttttt ctatttaagc agtgctttct
    10921 cttggactgg cttgactcat ggcattctac acgttattgc tggtctaaat gtgattttgc
    10981 caagcttctt caggacctat aattttgctt gacttgtagc caaacacaag taaaatgatt
    11041 aagcaacaaa tgtatttgtg aagcttggtt tttaggttgt tgtgttgtgt gtgcttgtgc
    11101 tctataataa tactatccag gggctggaga ggtggctcgg agttcaagag cacagactgc
    11161 tcttccagaa gtcctgagtt caattcccag caaccacatg gtggctcaca accatctgta
    11221 atgggatctg atgccctctt ctggtgtgtc tgaagaccac aagtgtattc acattaaata
    11281 aataaatcct ccttcttctt cttttttttt tttttaaaga gaatactgtc tccagtagaa
    11341 tttactgaag taatgaaata ctttgtgttt gttccaatat ggtagccaat aatcaaatta
    11401 ctctttaagc actggaaatg ttaccaagga actaattttt atttgaagtg taactgtgga
    11461 cagaggagcc ataactgcag acttgtggga tacagaagac caatgcagac tttaatgtct
    11521 tttctcttac actaagcaat aaagaaataa aaattgaact tctagtatcc tatttgttta
    11581 aactgctagc tttacttaac ttttgtgctt catctataca aagctgaaag ctaagtctgc
    11641 agccattact aaacatgaaa gcaagtaatg ataattttgg atttcaaaaa tgtagggcca
    11701 gagtttagcc agccagtggt ggtgcttgcc tttatgcctt taatcccagc actctggagg
    11761 cagagacagg cagatctctg agtttgagcc cagcctggtc tacacatcaa gttctatcta
    11821 ggatagccag gaatacacac agaaaccctg ttggggaggg gggctctgag atttcataaa
    11881 attataattg aagcattccc taatgagcca ctatggatgt ggctaaatcc gtctaccttt
    11941 ctgatgagat ttgggtatta ttttttctgt ctctgctgtt ggttgggtct tttgacactg
    12001 tgggctttct ttaaagcctc cttcctgcca tgtggtctct tgtttgctac taacttccca
    12061 tggcttaaat ggcatggctt tttgccttct aagggcagct gctgagattt gcagcctgat
    12121 ttccagggtg gggttgggaa atctttcaaa cactaaaatt gtcctttaat ttttttttta
    12181 aaaaatgggt tatataataa acctcataaa atagttatga ggagtgaggt ggactaatat
    12241 taaatgagtc cctcccctat aaaagagcta ttaaggcttt ttgtcttata cttaactttt
    12301 tttttaaatg tggtatcttt agaaccaagg gtcttagagt tttagtatac agaaactgtt
    12361 gcatcgctta atcagatttt ctagttcaaa tccagagaat ccaaattctt cacagccaaa
    12421 gtcaaattaa gaatttctga cttttaatgt taatttgctt actgtgaata taaaaatgat
    12481 agcttttcct gaggcagggt ctcactatgt atctctgcct gatctgcaac aagatatgta
    12541 gactaaagtt ctgcctgctt ttgtctcctg aatactaagg ttaaaatgta gtaatacttt
    12601 tggaacttgc aggtcagatt cttttatagg ggacacacta agggagcttg ggtgatagtt
    12661 ggtaaaatgt gtttcaagtg atgaaaactt gaattattat caccgcaacc tactttttaa
    12721 aaaaaaaagc caggcctgtt agagcatgct taagggatcc ctaggacttg ctgagcacac
    12781 aagagtagtt acttggcagg ctcctggtga gagcatattt caaaaaacaa ggcagacaac
    12841 caagaaacta cagttaaggt tacctgtctt taaaccatct gcatatacac agggatatta
    12901 aaatattcca aataatattt cattcaagtt ttcccccatc aaattgggac atggatttct
    12961 ccggtgaata ggcagagttg gaaactaaac aaatgttggt tttgtgattt gtgaaattgt
    13021 tttcaagtga tagttaaagc ccatgagata cagaacaaag ctgctatttc gaggtctctt
    13081 ggtttatact cagaagcact tctttgggtt tccctgcact atcctgatca tgtgctaggc
    13141 ctaccttagg ctgattgttg ttcaaataaa cttaagtttc ctgtcaggtg atgtcatatg
    13201 atttcatata tcaaggcaaa acatgttata tatgttaaac atttgtactt aatgtgaaag
    13261 ttaggtcttt tgggtttgat ttttaatttt caaaacctga gctaaataag tcatttttac
    13321 atgtcttaca tttggtggaa ttgtataatt gtggtttgca ggcaagactc tctgacctag
    13381 taaccctacc tatagagcac tttgctgggt cacaagtcta ggagtcaagc atttcacctt
    13441 gaagttgaga cgttttgtta gtgtatacta gtttatatgt tggaggacat gtttatccag
    13501 aagatattca ggactatttt tgactgggct aaggaattga ttctgattag cactgttagt
    13561 gagcattgag tggcctttag gcttgaattg gagtcacttg tatatctcaa ataatgctgg
    13621 ccttttttaa aaagcccttg ttctttatca ccctgttttc tacataattt ttgttcaaag
    13681 aaatacttgt ttggatctcc ttttgacaac aatagcatgt tttcaagcca tatttttttt
    13741 cctttttttt tttttttttg gtttttcgag acagggtttc tctgtatagc cctggctgtc
    13801 ctggaactca ctttgtagac caggctggcc tcgaactcag aaatccgcct gcctctgcct
    13861 cctgagtgcc gggattaaag gcgtgcacca ccacgcctgg ctaagttgga tattttgtta
    13921 tataactata accaatacta actccactgg gtggattttt aattcagtca gtagtcttaa
    13981 gtggtcttta ttggcccttc attaaaatct actgttcact ctaacagagg ctgttggtac
    14041 tagtggcact taagcaactt cctacggata tactagcaga ttaagggtca gggatagaaa
    14101 ctagtctagc gttttgtata cctaccagct ttatactacc ttgttctgat agaaatattt
    14161 caggacatct agcttatcga tccgtcgacg ttcgaacacg tggaattcct gcagcccggg
    14221 ggatccacta gttctagagc ggccgccacc gcggtggagc tccaattcgc cctatagtga
    14281 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
    14341 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
    14401 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
    14461 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    14521 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    14581 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    14641 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    14701 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    14761 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    14821 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    14881 gaattttaac aaaatattaa cgcttacaat ttag
//
