LOCUS       sequence-18236-e         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Chang C, Norris JD, Gron H, Paige LA, Hamilton PT, Kenan DJ, Fowlkes
            D, McDonnell DP
  TITLE     Dissection of the LXXLL nuclear receptor-coactivator interaction 
            motif using combinatorial peptide libraries: discovery of peptide 
            antagonists of estrogen receptors alpha and beta.
  JOURNAL   Mol Cell Biol. 1999 Dec . 19(12):8226-39.
  PUBMED    10567548
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1999 Dec . 19(12):8226-39."
COMMENT     Sequence Label: VP16-ER alpha
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      42..51
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             75..308
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 agtgtgaaga ggcttttttg gaggagatct aagctagcgc cgccaccatg ggccctaaaa
       61 agaagcgtaa agtcgccccc ccgaccgatg tcagcctggg ggacgagctc cacttagacg
      121 gcgaggacgt ggcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg
      181 gggacgggga ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc
      241 tggatatggc cgacttcgag tttgagcaga tgtttaccga tgcccttgga attgacgagt
      301 acggtgggga attcatgacc atgaccctcc acaccaaagc atctgggatg gccctactgc
      361 atcagatcca agggaacgag ctggagcccc tgaaccgtcc gcagctcaag atccccctgg
      421 agcggcccct gggcgaggtg tacctggaca gcagcaagcc cgccgtgtac aactaccccg
      481 agggcgccgc ctacgagttc aacgccgcgg ccgccgccaa cgcgcaggtc tacggtcaga
      541 ccggcctccc ctacggcccc gggtctgagg ctgcggcgtt cggctccaac ggcctggggg
      601 gtttcccccc actcaacagc gtgtctccga gcccgctgat gctactgcac ccgccgccgc
      661 agctgtcgcc tttcctgcag ccccacggcc agcaggtgcc ctactacctg gagaacgagc
      721 ccagcggcta cacggtgcgc gaggccggcc cgccggcatt ctacaggcca aattcagata
      781 atcgacgcca ggtg
//
