LOCUS       sequence-182383-       14877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGRG36-Kn-PA1-GFP is a Kanamycine resistance plasmid used to 
            integrate a GFP expression cassette into the chromosome of E.coli, 
            described in Yang et al. 2016 (Pending publication Pubmed ID).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14877)
  AUTHORS   Yang DD, Alexander A, Kinnersley M, Cook E, Caudy A, Rosebrock A, 
            Rosenzweig F
  TITLE     Fitness and Productivity Increase with Ecotypic Diversity among 
            Escherichia coli Strains That Coevolved in a Simple, Constant 
            Environment.
  JOURNAL   Appl Environ Microbiol. 2020 Apr 1;86(8). pii: AEM.00051-20. doi: 
            10.1128/AEM.00051-20. Print 2020 Apr 1.
  PUBMED    32060029
REFERENCE   2  (bases 1 to 14877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2020-04-1"; volume: "86(8). pii: 
            AEM.00051-20. doi"; pages: " 10.1128/AEM.00051-20. Print 2020 Apr 1"
COMMENT     Sequence Label: pGRG36-Kn-PA1-GFP
FEATURES             Location/Qualifiers
     source          1..14877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..717
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(31..59)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     654..674
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(838..856)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      852..899
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1317..2195)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2222..2506
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2429..2448
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(9197..9214)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      9367..9453
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9545..9572
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        9591..9682
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9867..10245
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(9901..9920)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             10592..11386
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10646..10665)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11256..11275
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11992..12011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            12034..12143
                     /note="incP origin of transfer"
     primer_bind     complement(12181..12200)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      12883..13105
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             13153..14103
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  14536..14701
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     RBS             14860..14871
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
       61 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      121 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      181 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      241 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      301 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      361 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      421 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      541 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      601 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      661 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaatagtaa
      721 ccaactccat aaggatccgc gatcgcggcg cgccacctgg tggccggccg gtaccacgcg
      781 tgcgcgctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      841 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      901 tgaaaggagg aactatatcc ggacatccac gccactcgag cacctaggag gcgcgccacg
      961 gccgcgtcga ccccacgccc ctctttaata cgacgggcaa tttgcacttc agaaaatgaa
     1021 gagtttgctt tagccataac aaaagtccag tatgcttttt cacagcataa ctggactgat
     1081 ttcagtttac aactattctg tctagtttaa gactttattg tcatagttta gatctatttt
     1141 gttcagttta agactttatt gtccgcccac aggccacgat gcgtccggcg tagaggatct
     1201 gctcatgttt gacagcttat catcgatgca taatgtgcct gtcaaatgga cgaagcaggg
     1261 attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat
     1321 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
     1381 ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca
     1441 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
     1501 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
     1561 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
     1621 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
     1681 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
     1741 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
     1801 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
     1861 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
     1921 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
     1981 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
     2041 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
     2101 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
     2161 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
     2221 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
     2281 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
     2341 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
     2401 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
     2461 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg
     2521 gctagcgaat tcgagctcgg tacccaagtc ttaaactaga cagaatagtt gtaaactgaa
     2581 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
     2641 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg
     2701 taaagactat attccatggc taacagtaca agaagttcct tcttcaggtc gttcccaccg
     2761 tatttattct cataagacgg gacgagtcca tcatttgcta tctgacttag agcttgctgt
     2821 ttttctcagt cttgagtggg agagcagcgt gctagatata cgcgagcagt tccccttatt
     2881 acctagtgat accaggcaga ttgcaataga tagtggtatt aagcatcctg ttattcgtgg
     2941 tgtagatcag gttatgtcta ctgatttttt agtggactgc aaagatggtc cttttgagca
     3001 gtttgctatt caagtcaaac ctgcagcagc cttacaagac gagcgtacct tagaaaaact
     3061 agaactagag cgtcgctatt ggcagcaaaa gcaaattcct tggttcattt ttactgataa
     3121 agaaataaat cccgtagtaa aagaaaatat tgaatggctt tattcagtga aaacagaaga
     3181 agtttctgcg gagcttttag cacaactatc cccattggcc catatcctgc aagaaaaagg
     3241 agatgaaaac attatcaatg tctgtaagca ggttgatatt gcttatgatt tggagttagg
     3301 caaaacattg agtgagatac gagccttaac cgcaaatggt tttattaagt tcaatattta
     3361 taagtctttc agggcaaata agtgtgcaga tctctgtatt agccaagtag tgaatatgga
     3421 ggagttgcgc tatgtggcaa attaatgagg ttgtgctatt tgataatgat ccgtatcgca
     3481 ttttggctat agaggatggc caagttgtct ggatgcaaat aagcgctgat aaaggagttc
     3541 cacaagctag ggctgagttg ttgctaatgc agtatttaga tgaaggccgc ttagttagaa
     3601 ctgatgaccc ttatgtacat cttgatttag aagagccgtc tgtagattct gtcagcttcc
     3661 agaagcgcga ggaggattat cgaaaaattc ttcctattat taatagtaag gatcgtttcg
     3721 accctaaagt cagaagcgaa ctcgttgagc atgtggtcca agaacataag gttactaagg
     3781 ctacagttta taagttgtta cgccgttact ggcagcgtgg tcaaacgcct aatgcattaa
     3841 ttcctgacta caaaaacagc ggtgcaccag gggaaagacg ttcagcgaca ggaacagcaa
     3901 agattggccg agccagagaa tatggtaagg gtgaaggaac caaggtaacg cccgagattg
     3961 aacgcctttt taggttgacc atagaaaagc acctgttaaa tcaaaaaggt acaaagacca
     4021 ccgttgccta tagacgattt gtggacttgt ttgctcagta ttttcctcgc attccccaag
     4081 aggattaccc aacactacgt cagtttcgtt atttttatga tcgagaatac cctaaagctc
     4141 agcgcttaaa gtctagagtt aaagcagggg tatataaaaa agacgtacga cccttaagta
     4201 gtacagccac ttctcaggcg ttaggccctg ggagtcgtta tgagattgat gccacgattg
     4261 ctgatattta tttagtggat catcatgatc gccaaaaaat cataggaaga ccaacgcttt
     4321 acattgtgat tgatgtgttt agtcggatga tcacgggctt ttatatcggc tttgaaaatc
     4381 cgtcttatgt ggtggcgatg caggcttttg taaatgcttg ctctgacaaa acggccattt
     4441 gtgcccagca tgatattgag attagtagct cagactggcc gtgtgtaggt ttgccagatg
     4501 tgttgctagc ggaccgtggc gaattaatga gtcatcaggt cgaagcctta gtttctagtt
     4561 ttaatgtgcg agtggaaagt gctccaccta gacgtggcga tgctaaaggc atagtggaaa
     4621 gcacttttag aacactacaa gccgagttta agtcctttgc acctggcatt gtagagggca
     4681 gtcggatcaa aagccatggt gaaacagact ataggttaga tgcatctctg tcggtatttg
     4741 agttcacaca aattattttg cgtacgatct tattcagaaa taaccatctg gtgatggata
     4801 aatacgatcg agatgctgat tttcctacag atttaccgtc tattcctgtc cagctatggc
     4861 aatggggtat gcagcatcgt acaggtagtt taagggctgt ggagcaagag cagttgcgag
     4921 tagcgttact gcctcgccga aaggtctcta tttcttcatt tggcgttaat ttgtggggtt
     4981 tgtattactc ggggtcagag attctgcgtg agggttggtt gcagcggagc actgatatag
     5041 ctagacctca acatttagaa gcggcttatg acccagtgct ggttgatacg atttatttgt
     5101 ttccgcaagt tggcagccgt gtattttggc gctgtaatct gacggaacgt agtcggcagt
     5161 ttaaaggtct ctcattttgg gaggtttggg atatacaagc acaagaaaaa cacaataaag
     5221 ccaatgcgaa gcaggatgag ttaactaaac gcagggagct tgaggcgttt attcagcaaa
     5281 ccattcagaa agcgaataag ttaacgccca gtactactga gcccaaatca acacgcatta
     5341 agcagattaa aactaataaa aaagaagccg tgacctcgga gcgtaaaaaa cgtgcggagc
     5401 atttgaagcc aagctcttca ggtgatgagg ctaaagttat tcctttcaac gcagtggaag
     5461 cggatgatca agaagattac agcctaccca catacgtgcc tgaattattt caggatccac
     5521 cagaaaagga tgagtcatga gtgctacccg gattcaagca gtttatcgtg atacgggggt
     5581 agaggcttat cgtgataatc cttttatcga ggccttacca ccattacaag agtcagtgaa
     5641 tagtgctgca tcactgaaat cctctttaca gcttacttcc tctgacttgc aaaagtcccg
     5701 tgttatcaga gctcatacca tttgtcgtat tccagatgac tattttcagc cattaggtac
     5761 gcatttgcta ctaagtgagc gtatttcggt catgattcga ggtggctacg taggcagaaa
     5821 tcctaaaaca ggagatttac aaaagcattt acaaaatggt tatgagcgtg ttcaaacggg
     5881 agagttggag acatttcgct ttgaggaggc acgatctacg gcacaaagct tattgttaat
     5941 tggttgttct ggtagtggga agacgacctc tcttcatcgt attctagcca cgtatcctca
     6001 ggtgatttac catcgtgaac tcaatgtaga gcaggtggtg tatttgaaaa tagactgctc
     6061 gcataatggt tcgctaaaag aaatctgctt gaattttttc agagcgttgg atcgagcctt
     6121 gggctcgaac tatgagcgtc gttatggctt aaaacgtcat ggtatagaaa ccatgttggc
     6181 tttgatgtcg caaatagcca atgcacatgc tttagggttg ttggttattg atgaaattca
     6241 gcatttaagc cgctctcgtt cgggtggatc tcaagagatg ctgaactttt ttgtgacgat
     6301 ggtgaatatt attggcgtac cagtgatgtt gattggtacc cctaaagcac gagagatttt
     6361 tgaggctgat ttgcggtctg cacgtagagg ggcagggttt ggagctatat tctgggatcc
     6421 tatacaacaa acgcaacgtg gaaagcccaa tcaagagtgg atcgctttta cggataatct
     6481 ttggcaatta cagcttttac aacgcaaaga tgcgctgtta tcggatgagg tccgtgatgt
     6541 gtggtatgag ctaagccaag gagtgatgga cattgtagta aaactttttg tactcgctca
     6601 gctccgtgcg ctagctttag gcaatgagcg tattaccgct ggtttattgc ggcaagtgta
     6661 tcaagatgag ttaaagcctg tgcaccccat gctagaggca ttacgctcgg gtatcccaga
     6721 acgcattgct cgttattctg atctagtcgt tcccgagatt gataaacggt taatccaact
     6781 tcagctagat atcgcagcga tacaagaaca aacaccagaa gaaaaagccc ttcaagagtt
     6841 agataccgaa gatcagcgtc atttatatct gatgctgaaa gaggattacg attcaagcct
     6901 gttaattccc actattaaaa aagcgtttag ccagaatcca acgatgacaa gacaaaagtt
     6961 actgcctctt gttttgcagt ggttgatgga aggcgaaacg gtagtgtcag aactagaaaa
     7021 gccctccaag agtaaaaagg tttcggctat aaaggtagtc aagcccagcg actgggatag
     7081 cttgcctgat acggatttac gttatatcta ttcacaacgc caacctgaaa aaaccatgca
     7141 tgaacggtta aaagggaaag gggtaatagt ggatatggcg agcttattta aacaagcagg
     7201 ttagccatga gaaactttcc tgttccgtac tcgaatgagc tgatttatag cactattgca
     7261 cgggcaggcg tttatcaagg gattgttagt cctaagcagc tgttggatga ggtgtatggc
     7321 aaccgcaagg tggtcgctac cttaggtctg ccctcgcatt taggtgtgat agcaagacat
     7381 ctacatcaaa caggacgtta cgctgttcag cagcttattt atgagcatac cttattccct
     7441 ttatatgctc cgtttgtagg caaggagcgc cgagacgaag ctattcggtt aatggagtac
     7501 caagcgcaag gtgcggtgca tttaatgcta ggagtcgctg cttctagagt taagagcgat
     7561 aaccgcttta gatactgccc tgattgcgtt gctcttcagc taaataggta tggggaagcc
     7621 ttttggcaac gagattggta tttgcccgct ttgccatatt gtccaaaaca cggtgcttta
     7681 gtcttctttg atagagctgt agatgatcac cgacatcaat tttgggcttt gggtcatact
     7741 gagctgcttt cagactaccc caaagactcc ctatctcaat taacagcact agctgcttat
     7801 atagcccctc tgttagatgc tccacgagcg caagagcttt ccccaagcct tgagcagtgg
     7861 acgctgtttt atcagcgctt agcgcaggat ctagggctaa ccaaaagcaa gcacattcgt
     7921 catgacttgg tggcggagag agtgaggcag acttttagtg atgaggcact agagaaactg
     7981 gatttaaagt tggcagagaa caaggacacg tgttggctga aaagtatatt ccgtaagcat
     8041 agaaaagcct ttagttattt acagcatagt attgtgtggc aagccttatt gccaaaacta
     8101 acggttatag aagcgctaca gcaggcaagt gctcttactg agcactctat aacgacaaga
     8161 cctgttagcc agtctgtgca acctaactct gaagatttat ctgttaagca taaagactgg
     8221 cagcaactag tgcataaata ccaaggaatt aaggcggcaa gacagtcttt agagggtggg
     8281 gtgctatacg cttggcttta ccgacatgac agggattggc tagttcactg gaatcaacag
     8341 catcaacaag agcgtctggc acccgcccct agagttgatt ggaaccaaag agatcgaatt
     8401 gctgtacgac aactattaag aatcataaag cgtctagata gtagccttga tcacccaaga
     8461 gcgacatcga gctggctgtt aaagcaaact cctaacggaa cctctcttgc aaaaaatcta
     8521 cagaaactgc ctttggtagc gctttgctta aagcgttact cagagagtgt ggaagattat
     8581 caaattagac ggattagcca agcttttatt aagcttaaac aggaagatgt tgagcttagg
     8641 cgctggcgat tattaagaag tgcaacgtta tctaaagagc ggataactga ggaagcacaa
     8701 agattcttgg aaatggttta tggggaagag tgagtggtta ggctagctac atttaatgac
     8761 aatgtgcagg ttgtacatat tggtcattta ttccgtaact cgggtcataa ggagtggcgt
     8821 atttttgttt ggtttaatcc aatgcaagaa cggaaatgga ctcgatttac tcatttgcct
     8881 ttattaagtc gagctaaggt ggttaacagt acaacaaagc aaataaataa ggcggatcgt
     8941 gtgattgagt ttgaagcatc ggatcttcaa cgagccaaaa taatcgattt tcctaatctc
     9001 tcgtcctttg cttccgtacg caacaaggat ggagcgcaga gttcatttat ttacgaagct
     9061 gaaacaccat atagcaagac tcgttatcac atcccacagt tagagctagc tcggtcatta
     9121 tttttagggg atcctctaga gtcgacctgc aggcatgcaa gcttggctgt tttggcggat
     9181 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     9241 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     9301 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     9361 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     9421 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     9481 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
     9541 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
     9601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     9661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     9721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     9781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     9841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     9901 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     9961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    10021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    10081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    10141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    10201 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgcc gcaagcactc
    10261 agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg
    10321 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
    10381 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
    10441 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
    10501 caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc
    10561 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
    10621 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
    10681 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
    10741 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
    10801 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
    10861 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
    10921 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
    10981 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
    11041 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
    11101 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
    11161 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
    11221 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
    11281 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
    11341 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
    11401 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
    11461 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    11521 cagcgcgggg atctcatgct ggagttcttc gcccacccca gcttcaagca aactattaac
    11581 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    11641 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    11701 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    11761 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    11821 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    11881 ctcatatata ctttagattg atttacgcgc cctgtagcgg cgcattaagc gcggcgggtg
    11941 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    12001 ctttcttccc ttcctttctc gccacgttcg ccgccggcca gcctcgcaga gcaggattcc
    12061 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
    12121 ggcctacttc acctatcctg cccggcggca tcaccggcgc cacaggtgcg gttgctggcg
    12181 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
    12241 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
    12301 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
    12361 gcttcctaat gcaggagtcg cataagggag agcgtcgatc cccgacagta agacgggtaa
    12421 gcctgttgat gataccgctg ccttactggg tgcattagcc agtctgaatg acctgtcacg
    12481 ggataatccg aagtggtcag actggaaaat cagagggcag gaactgctga acagcaaaaa
    12541 gtcagatagc accacatagc agacccgcca taaaacgccc tgagaagccc gtgacgggct
    12601 tttcttgtat tatgggtagt ttccttgcat gaatccataa aaggcgcctg tagtgccatt
    12661 tacccccatt cactgccaga gccgtgagcg cagcgaactg aatgtcacga aaaagacagc
    12721 gactcaggtg cctgatggtc ggagacaaaa ggaatattca gcgatttgcc cgagcttgcg
    12781 agggtgctac ttaagccttt agggttttaa ggtctgtttt gtagaggagc aaacagcgtt
    12841 tgcgacatcc ttttgtaata ctgcggaact gactaaagta gtgagttata cacagggctg
    12901 ggatctattc tttttatctt tttttattct ttctttattc tataaattat aaccacttga
    12961 atataaacaa aaaaaacaca caaaggtcta gcggaattta cagagggtct agcagaattt
    13021 acaagttttc cagcaaaggt ctagcagaat ttacagatac ccacaactca aaggaaaagg
    13081 actagtaatt atcattgact agcccatctc aattggtata gtgattaaaa tcacctagac
    13141 caattgagat gtatgtctga attagttgtt ttcaaagcaa atgaactagc gattagtcgc
    13201 tatgacttaa cggagcatga aaccaagcta attttatgct gtgtggcact actcaacccc
    13261 acgattgaaa accctacaag gaaagaacgg acggtatcgt tcacttataa ccaatacgtt
    13321 cagatgatga acatcagtag ggaaaatgct tatggtgtat tagctaaagc aaccagagag
    13381 ctgatgacga gaactgtgga aatcaggaat cctttggtta aaggctttga gattttccag
    13441 tggacaaact atgccaagtt ctcaagcgaa aaattagaat tagtttttag tgaagagata
    13501 ttgccttatc ttttccagtt aaaaaaattc ataaaatata atctggaaca tgttaagtct
    13561 tttgaaaaca aatactctat gaggatttat gagtggttat taaaagaact aacacaaaag
    13621 aaaactcaca aggcaaatat agagattagc cttgatgaat ttaagttcat gttaatgctt
    13681 gaaaataact accatgagtt taaaaggctt aaccaatggg ttttgaaacc aataagtaaa
    13741 gatttaaaca cttacagcaa tatgaaattg gtggttgata agcgaggccg cccgactgat
    13801 acgttgattt tccaagttga actagataga caaatggatc tcgtaaccga acttgagaac
    13861 aaccagataa aaatgaatgg tgacaaaata ccaacaacca ttacatcaga ttcctaccta
    13921 cataacggac taagaaaaac actacacgat gctttaactg caaaaattca gctcaccagt
    13981 tttgaggcaa aatttttgag tgacatgcaa agtaagtatg atctcaatgg ttcgttctca
    14041 tggctcacgc aaaaacaacg aaccacacta gagaacatac tggctaaata cggaaggatc
    14101 tgaggttctt atggctcttg tatctatcag tgaagcatca agactaacaa acaaaagtag
    14161 aacaactgtt caccgttaca tatcaaaggg aaaactgtcc atatgcacag atgaaaacgg
    14221 tgtaaaaaag atagatacat cagagctttt acgagttttt ggtgcattta aagctgttca
    14281 ccatgaacag atcgacaatg taacagatga acagcatgta acacctaata gaacaggtga
    14341 aaccagtaaa acaaagcaac tagaacatga aattgaacac ctgagacaac ttgttacagc
    14401 tcaacagtca cacatagaca gcctgaaaca ggcgatgctg cttatcgaat caaagctgcc
    14461 gacaacacgg gagccagtga cgcctcccgt ggggaaaaaa tcatggcaat tctggaagaa
    14521 atagcgcttt cagcctgtgg gcggacaaaa tagttgggaa ctgggagggg tggaaatgga
    14581 gtttttaagg attatttagg gaagagtgac aaaatagatg ggaactgggt gtagcgtcgt
    14641 aagctaatac gaaaattaaa aatgacaaaa tagtttggaa ctagatttca cttatctggt
    14701 tggtcgacac tagtattacc ctgttatccc tagatttaaa tgatatcgga tcctagtaag
    14761 ccacgtttta attaatcaga tcccgggtca atagcggtta tcaaaaagag tattgactta
    14821 aagtctaacc tataggatac ttacagccat cgagagatta aagaggagaa aggcgaa
//
