LOCUS       sequence-182384-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian overexpression vector for TRIM28; catalytically inactive 
            (mutated on C65A and C68A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, 
            Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
            Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
  TITLE     TRIM28 regulates the nuclear accumulation and toxicity of both 
            alpha-synuclein and tau.
  JOURNAL   Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
  PUBMED    27779468
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-10-25"; volume: "5. pii: e19809. doi"; pages: " 
            10.7554/eLife.19809"
COMMENT     Sequence Label: pKH3-TRIM28-C65A/C68A
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..32
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        242..260
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     242..259
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     273..292
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             353..379
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             386..412
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             419..445
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
       61 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
      121 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc
      181 ccttggcttc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
      241 gatttaggtg acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc
      301 ccaggtccaa ctgcacctcg gttctaagct tctgcaggtc gactctagaa tgtacccata
      361 cgatgttcca gattacgctg gatcttaccc atacgatgtt ccagattacg ctggatctta
      421 cccatacgat gttccagatt acgctggatc tatggcggcc tccgcggcgg cagcctcggc
      481 agcagcggcc tcggccgcct ctggcagccc gggcccgggc gagggctccg ctggcggcga
      541 aaagcgctcc accgcccctt cggccgcagc ctcggcctct gcctcagccg cggcgtcgtc
      601 gcccgcgggg ggcggcgccg aggcgctgga gctgctggag cacgccggcg tggccagaga
      661 gcgcctgcga cccgagaggg agccccgcct gctgccctgt ttgcactcgg cctgtagtgc
      721 ctgcttaggg cccgcggccc ccgccgccgc caacagctcg ggggacggcg gggcggcggg
      781 cgacggcacc gtggtggact gtcccgtgtg caagcaacag tgcttctc
//
