LOCUS       sequence-182405-       13685 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant 
            Selection: 2x35S:bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pAtCas9-dead_3
FEATURES             Location/Qualifiers
     source          1..13685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2389..3065)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3256..3277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3292..3297,3298..3315,3316..3322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3330..3346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3335..3357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3354..3370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3354..3370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3900..4245
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(4279..4305,4306..4308,4309..6795,6796..6798,
                     6799..8382)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8395..8415
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8426..8674
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(8696..8713)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8696..8712)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8705..8727)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8915..8939
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10240..10869
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11298..12371
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12437..12631
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12867..12889)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12975..13115
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13030..13049
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13130..13147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(13301..13685,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13381..13400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcatggtaga ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
     2401 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     2461 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     2521 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     2581 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     2641 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     2701 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     2761 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
     2821 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     2881 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     2941 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     3001 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     3061 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     3121 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
     3181 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3241 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3301 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     3361 cagctatgac atgattacga attcgagctc ggtacccggg gatcggcgcg ccagatttgc
     3421 cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta cgcagcaggt
     3481 atcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct tcccaagaag
     3541 gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata
     3601 tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct tcacaaacca
     3661 aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg
     3721 gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc
     3781 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac
     3841 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt
     3901 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
     3961 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
     4021 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
     4081 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     4141 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     4201 gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg gactcctgca
     4261 ggtagatcgc tcgtcgacat ggataagaag tactctatcg gactcgctat cggaactaac
     4321 tctgtgggat gggctgtgat caccgatgag tacaaggtgc catctaagaa gttcaaggtt
     4381 ctcggaaaca ccgataggca ctctatcaag aaaaacctta tcggtgctct cctcttcgat
     4441 tctggtgaaa ctgctgaggc taccagactc aagagaaccg ctagaagaag gtacaccaga
     4501 agaaagaaca ggatctgcta cctccaagag atcttctcta acgagatggc taaagtggat
     4561 gattcattct tccacaggct cgaagagtca ttcctcgtgg aagaagataa gaagcacgag
     4621 aggcacccta tcttcggaaa catcgttgat gaggtggcat accacgagaa gtaccctact
     4681 atctaccacc tcagaaagaa gctcgttgat tctactgata aggctgatct caggctcatc
     4741 tacctcgctc tcgctcacat gatcaagttc agaggacact tcctcatcga gggtgatctc
     4801 aaccctgata actctgatgt ggataagttg ttcatccagc tcgtgcagac ctacaaccag
     4861 cttttcgaag agaaccctat caacgcttca ggtgtggatg ctaaggctat cctctctgct
     4921 aggctctcta agtcaagaag gcttgagaac ctcattgctc agctccctgg tgagaagaag
     4981 aacggacttt tcggaaactt gatcgctctc tctctcggac tcacccctaa cttcaagtct
     5041 aacttcgatc tcgctgagga tgcaaagctc cagctctcaa aggataccta cgatgatgat
     5101 ctcgataacc tcctcgctca gatcggagat cagtacgctg atttgttcct cgctgctaag
     5161 aacctctctg atgctatcct cctcagtgat atcctcagag tgaacaccga gatcaccaag
     5221 gctccactct cagcttctat gatcaagaga tacgatgagc accaccagga tctcacactt
     5281 ctcaaggctc ttgttagaca gcagctccca gagaagtaca aagagatttt cttcgatcag
     5341 tctaagaacg gatacgctgg ttacatcgat ggtggtgcat ctcaagaaga gttctacaag
     5401 ttcatcaagc ctatcctcga gaagatggat ggaaccgagg aactcctcgt gaagctcaat
     5461 agagaggatc ttctcagaaa gcagaggacc ttcgataacg gatctatccc tcatcagatc
     5521 cacctcggag agttgcacgc tatccttaga aggcaagagg atttctaccc attcctcaag
     5581 gataacaggg aaaagattga gaagattctc accttcagaa tcccttacta cgtgggacct
     5641 ctcgctagag gaaactcaag attcgcttgg atgaccagaa agtctgagga aaccatcacc
     5701 ccttggaact tcgaagaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg
     5761 atgaccaact tcgataagaa ccttccaaac gagaaggtgc tccctaagca ctctttgctc
     5821 tacgagtact tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg
     5881 aggaagcctg cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag
     5941 accaacagaa aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc
     6001 ttcgattcag ttgagatttc tggtgttgag gataggttca acgcatctct cggaacctac
     6061 cacgatctcc tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat
     6121 atcttggagg atatcgttct taccctcacc ctctttgaag atagagagat gattgaagaa
     6181 aggctcaaga cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga
     6241 agatacactg gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag
     6301 tctggaaaga caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg
     6361 cagctcatcc acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca
     6421 ggacagggtg atagtctcca tgagcatatc gctaacctcg ctggatctcc tgcaatcaag
     6481 aagggaatcc tccagactgt gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat
     6541 aagcctgaga acatcgtgat cgaaatggct agagagaacc agaccactca gaagggacag
     6601 aagaactcta gggaaaggat gaagaggatc gaggaaggta tcaaagagct tggatctcag
     6661 atcctcaaag agcaccctgt tgagaacact cagctccaga atgagaagct ctacctctac
     6721 tacctccaga acggaaggga tatgtatgtg gatcaagagt tggatatcaa caggctctct
     6781 gattacgatg ttgatgctat cgtgccacag tcattcttga aggatgattc tatcgataac
     6841 aaggtgctca ccaggtctga taagaacagg ggtaagagtg ataacgtgcc aagtgaagag
     6901 gttgtgaaga aaatgaagaa ctattggagg cagctcctca acgctaagct catcactcag
     6961 agaaagttcg ataacttgac taaggctgag aggggaggac tctctgaatt ggataaggca
     7021 ggattcatca agaggcagct tgtggaaacc aggcagatca ctaagcacgt tgcacagatc
     7081 ctcgattcta ggatgaacac caagtacgat gagaacgata agttgatcag ggaagtgaag
     7141 gttatcaccc tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca attctacaag
     7201 gtgagggaaa tcaacaacta ccaccacgct cacgatgctt accttaacgc tgttgttgga
     7261 accgctctca tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg tgattacaag
     7321 gtgtacgatg tgaggaagat gatcgctaag tctgagcaag agatcggaaa ggctaccgct
     7381 aagtatttct tctactctaa catcatgaat ttcttcaaga ccgagattac cctcgctaac
     7441 ggtgagatca gaaagaggcc actcatcgag acaaacggtg aaacaggtga gatcgtgtgg
     7501 gataagggaa gggatttcgc taccgttaga aaggtgctct ctatgccaca ggtgaacatc
     7561 gttaagaaaa ccgaggtgca gaccggtgga ttctctaaag agtctatcct ccctaagagg
     7621 aactctgata agctcattgc taggaagaag gattgggacc ctaagaaata cggtggtttc
     7681 gattctccta ccgtggctta ctctgttctc gttgtggcta aggttgagaa gggaaagagt
     7741 aagaagctca agtctgttaa ggaacttctc ggaatcacta tcatggaaag gtcatctttc
     7801 gagaagaacc caatcgattt cctcgaggct aagggataca aagaggttaa gaaggatctc
     7861 atcatcaagc tcccaaagta ctcactcttc gaactcgaga acggtagaaa gaggatgctc
     7921 gcttctgctg gtgagcttca aaagggaaac gagcttgctc tcccatctaa gtacgttaac
     7981 tttctttacc tcgcttctca ctacgagaag ttgaagggat ctccagaaga taacgagcag
     8041 aagcaacttt tcgttgagca gcacaagcac tacttggatg agatcatcga gcagatctct
     8101 gagttctcta aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac
     8161 aacaagcaca gagataagcc tatcagggaa caggcagaga acatcatcca tctcttcacc
     8221 cttaccaacc tcggtgctcc tgctgctttc aagtacttcg atacaaccat cgataggaag
     8281 agatacacct ctaccaaaga agtgctcgat gctaccctca tccatcagtc tatcactgga
     8341 ctctacgaga ctaggatcga tctctcacag ctcggtggtg attcaagggc tgatcctaag
     8401 aagaagagga aggtttgact cgagatatga agatgaagat gaaatatttg gtgtgtcaaa
     8461 taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt
     8521 ggctttttct aatattaaat gaatgtaaga tcacattata atgaataaac aaatgtttct
     8581 ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct
     8641 atggtatgga ctatggaata tgattaaaga taaggagctc cggtgacgga ctggcactgg
     8701 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     8761 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     8821 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
     8881 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     8941 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     9001 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     9061 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     9121 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     9181 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     9241 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     9301 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     9361 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     9421 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     9481 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     9541 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     9601 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     9661 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     9721 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     9781 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     9841 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     9901 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     9961 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
    10021 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
    10081 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
    10141 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
    10201 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
    10261 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
    10321 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
    10381 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
    10441 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
    10501 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
    10561 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
    10621 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
    10681 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
    10741 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
    10801 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
    10861 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
    10921 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
    10981 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
    11041 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
    11101 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
    11161 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
    11221 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc
    11281 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
    11341 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
    11401 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
    11461 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
    11521 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
    11581 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
    11641 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
    11701 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
    11761 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
    11821 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
    11881 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
    11941 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
    12001 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
    12061 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
    12121 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
    12181 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
    12241 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
    12301 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
    12361 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
    12421 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
    12481 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
    12541 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
    12601 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gcccaagagc
    12661 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
    12721 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
    12781 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
    12841 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    12901 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    12961 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
    13021 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    13081 cgcacagatg cgtaaggaga aaataccgca tcaggccctc ttccgcttcc tcgctcactg
    13141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    13201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    13261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    13321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    13381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    13441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    13501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    13561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    13621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    13681 ggtat
//
