LOCUS       sequence-182409-       15875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: 
            PvUbi2:bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15875)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 15875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTaCas9_6
FEATURES             Location/Qualifiers
     source          1..15875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    4284..4305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4320..4325,4326..4343,4344..4350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4358..4374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4363..4385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4382..4398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4382..4398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6454..10557
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6454..10557
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10558..10605
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      10616..10864
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(10886..10903)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10886..10902)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10895..10917)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    11105..11129
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12430..13059
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13488..14561
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14627..14821
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15057..15079)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15165..15305
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15220..15239
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15320..15337)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(15491..15875,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15571..15590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtgga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcataagctt ctgcaaaaga gaaccagaca acagggtaag tgcctagcag taaacaaaca
     2401 gaactcatca caagcaaaca gcaacatcat attcatacca acaggtcatg tgtgttcatc
     2461 acatcattag tactaagcat gccatcatcc aagtatatca aagtaagggc aaagagcatg
     2521 catgatcatc aggtgcacaa aagaatcatc aaattgtagc agtacatatc ttcatctatc
     2581 atgcatatct atccataaca ggacgatgca tgttgaccag gtaaaagcta caggatccta
     2641 taggaacagc agcgtatata tcttcaccaa tcttagcatc ttaatcatgt ggcacatgca
     2701 gtttcaattt aagcacatga gctagttgat tatgaggtac cagagaatca tcaaactgat
     2761 gtagcagcat atatcctcat ctatcatgca atctaatcta atctaactaa acaggaaagg
     2821 tgtgctattc agttaaaagc taccgcatca tatacaaacg gcagcataag aaaaagcata
     2881 atcatcttaa tcatgcaaca aacgcagatt cataataagc ccaagagcta gcttgtgatg
     2941 atcttattct actctgatct acagcaatca gataacgacc taaccttgca catggcaaca
     3001 aaacaatcga tcggacgaat cagttgtttg ttcctagcta gcaccatcga accagataat
     3061 agatgcacgt acagatcccg aaaacgaacc caaaaacagg gcagacctag ctgaacctag
     3121 gcagcgaccc agcagatcgt gagaacgatc tcatctacga acagcctaga agcaacccca
     3181 cgattcccgg acaaacgacc taaaatcccc acaaatcaca tgagcatgac aggataaaca
     3241 gcggaaccga tcagatctac acgaaaaccc cacctcccag ccacccacga tcaggaaaca
     3301 cgcggatcta gcatgatttc gtcaacgcct cagcctagtt cctagccaca gaccaagcag
     3361 aaccaccaaa ccacgccgag cgaggagatg gggcaagagg acgggggaga cgatcgccgt
     3421 accttgaagc gggggaagga tcgccgaggg tcgcgaggag agcaatttgg atttggaacc
     3481 cgggggttgt gcgctccgaa cgatgagacg atgtgagatt gtgggaagag gcgcggaggg
     3541 ccctgtattt atgggctgcg acggggggag gagaggtggg gagggttggg gaaggaatcc
     3601 cccacccgtg ccgtgacggt tccgggccgt gtgagaggag cccgctcgtc tccgccacgc
     3661 aatttccgcg atcggagcgg agctttcgag aggcggctgg atggttggtg gccgttagat
     3721 ttgtagacgc cgttaacgcc tcgcctccac cgggaagagt tttgagcagc cgcttatgac
     3781 aatggcttaa cgacgttaga cggagcgtta gtggcaggcc atgatagaat agacgtattg
     3841 caatgggatt ataattaaat aaataagaat ataataagat atggcaagtc ggcactcatg
     3901 acatggtctt cgaaatgata gtgctcactt tcttagccga gaaagttgac gcgactgatt
     3961 tagaagttaa gatattattt ctctcttctt ttctttcctc gtcatataag gatgaaataa
     4021 actttagaga ttgccggtag tgattttgga tttcggcgat caggcttggt ttgccggttt
     4081 cggacggtgt gccttaggcc acccgcagtg tatcttgtaa tgttcaaccg ataagcaagg
     4141 gtggggctca agcaagtagt aaacaactat gtcaaatgtc accatggtta tggtcttgtt
     4201 tagttggctt cttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     4321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     4381 acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga tctgcaggcg
     4441 cgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag cattgcatgt
     4501 ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag tgcagtttat
     4561 ctatctttat acatatattt aaactttact ctacgaataa tataatctat agtactacaa
     4621 taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct aaaggacaat
     4681 tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt
     4741 tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt
     4801 agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat
     4861 tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt aataatttag
     4921 atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt aagaaattaa
     4981 aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt taaacgccgt
     5041 cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc
     5101 agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc gctccaccgt
     5161 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
     5221 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
     5281 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
     5341 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
     5401 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccctctctac
     5461 cttctctaga tcggcgttcc ggtccatggt tagggcccgg tagttctact tctgttcatg
     5521 tttgtgttag atccgtgttt gtgttagatc cgtgctgcta gcgttcgtac acggatgcga
     5581 cctgtacgtc agacacgttc tgattgctaa cttgccagtg tttctctttg gggaatcctg
     5641 ggatggctct agccgttccg cagacgggat cgatttcatg attttttttg tttcgttgca
     5701 tagggtttgg tttgcccttt tcctttattt caatatatgc cgtgcacttg tttgtcgggt
     5761 catcttttca tgcttttttt tgtcttggtt gtgatgatgt ggtctggttg ggcggtcgtt
     5821 ctagatcgga gtagaattaa ttctgtttca aactacctgg tggatttatt aattttggat
     5881 ctgtatgtgt gtgccataca tattcatagt tacgaattga agatgatgga tggaaatatc
     5941 gatctaggat aggtatacat gttgatgcgg gttttactga tgcatataca gagatgcttt
     6001 ttgttcgctt ggttgtgatg atgtggtgtg gttgggcggt cgttcattcg ttctagatcg
     6061 gagtagaata ctgtttcaaa ctacctggtg tatttattaa ttttggaact gtatgtgtgt
     6121 gtcatacatc ttcatagtta cgagtttaag atggatggaa atatcgatct aggataggta
     6181 tacatgttga tgtgggtttt actgatgcat atacatgatg gcatatgcag catctattca
     6241 tatgctctaa ccttgagtac ctatctatta taataaacaa gtatgtttta taattatttt
     6301 gatcttgata tacttggatg atggcatatg cagcagctat atgtggattt ttttagccct
     6361 gccttcatac gctatttatt tgcttggtac tgtttctttt gtcgatgctc accctgttgt
     6421 ttggtgttac ttcctgcagg gcgatctatt cgaatggaca agaagtactc gatcggcctc
     6481 gacatcggga cgaactcagt tggctgggcc gtgatcaccg acgagtacaa ggtgccctct
     6541 aagaagttca aggtcctggg gaacaccgac cgccattcca tcaagaagaa cctcatcggc
     6601 gctctcctgt tcgacagcgg ggagaccgct gaggctacga ggctcaagag aaccgctagg
     6661 cgccggtaca cgagaaggaa gaacaggatc tgctacctcc aagagatttt ctccaacgag
     6721 atggccaagg ttgacgattc attcttccac cgcctggagg agtctttcct cgtggaggag
     6781 gataagaagc acgagcggca tcccatcttc ggcaacatcg tggacgaggt tgcctaccac
     6841 gagaagtacc ctacgatcta ccatctgcgg aagaagctcg tggactccac cgataaggcg
     6901 gacctcagac tgatctacct cgctctggcc cacatgatca agttccgcgg ccatttcctg
     6961 atcgaggggg atctcaaccc agacaacagc gatgttgaca agctgttcat ccaactcgtg
     7021 cagacctaca accaactctt cgaggagaac ccgatcaacg cctctggcgt ggacgcgaag
     7081 gctatcctgt ccgcgaggct ctcgaagtcc aggaggctgg agaacctgat cgctcagctc
     7141 ccaggcgaga agaagaacgg cctgttcggg aacctcatcg ctctcagcct ggggctcacc
     7201 ccgaacttca agtcgaactt cgatctcgct gaggacgcca agctgcaact ctccaaggac
     7261 acctacgacg atgacctcga taacctcctg gcccagatcg gcgatcaata cgcggacctg
     7321 ttcctcgctg ccaagaacct gtcggacgcc atcctcctgt cagatatcct ccgcgtgaac
     7381 accgagatca cgaaggctcc actctctgcc tccatgatca agcgctacga cgagcaccat
     7441 caggatctga ccctcctgaa ggcgctggtc cgccaacagc tcccggagaa gtacaaggag
     7501 attttcttcg atcagtcgaa gaacggctac gctgggtaca tcgacggcgg ggcctcacaa
     7561 gaggagttct acaagttcat caagccaatc ctggagaaga tggacggcac ggaggagctc
     7621 ctggtgaagc tcaacaggga ggacctcctg cggaagcaga gaaccttcga taacggcagc
     7681 atcccccacc aaatccatct cggggagctg cacgccatcc tgagaaggca agaggacttc
     7741 taccctttcc tcaaggataa ccgggagaag atcgagaaga tcctgacctt cagaatccca
     7801 tactacgtcg gccctctcgc gcgggggaac tcaagattcg cttggatgac ccgcaagtct
     7861 gaggagacca tcacgccgtg gaacttcgag gaggtggtgg acaagggcgc tagcgctcag
     7921 tcgttcatcg agaggatgac caacttcgac aagaacctgc ccaacgagaa ggtgctccct
     7981 aagcactcgc tcctgtacga gtacttcacc gtctacaacg agctcacgaa ggtgaagtac
     8041 gtcaccgagg gcatgcgcaa gccagcgttc ctgtccgggg agcagaagaa ggctatcgtg
     8101 gacctcctgt tcaagaccaa ccggaaggtc acggttaagc aactcaagga ggactacttc
     8161 aagaagatcg agtgcttcga ttcggtcgag atcagcggcg ttgaggaccg cttcaacgcc
     8221 agcctcggga cctaccacga tctcctgaag atcatcaagg ataaggactt cctggacaac
     8281 gaggagaacg aggatatcct ggaggacatc gtgctgaccc tcacgctgtt cgaggacagg
     8341 gagatgatcg aggagcgcct gaagacgtac gcccatctct tcgatgacaa ggtcatgaag
     8401 caactcaagc gccggagata caccggctgg gggaggctgt cccgcaagct catcaacggc
     8461 atccgggaca agcagtccgg gaagaccatc ctcgacttcc tgaagagcga tggcttcgcc
     8521 aacaggaact tcatgcaact gatccacgat gacagcctca ccttcaagga ggatatccaa
     8581 aaggctcaag tgagcggcca gggggactcg ctgcacgagc atatcgcgaa cctcgctggc
     8641 tcccccgcga tcaagaaggg catcctccag accgtgaagg ttgtggacga gctcgtgaag
     8701 gtcatgggcc ggcacaagcc tgagaacatc gtcatcgaga tggccagaga gaaccaaacc
     8761 acgcagaagg ggcaaaagaa ctctagggag cgcatgaagc gcatcgagga gggcatcaag
     8821 gagctggggt cccaaatcct caaggagcac ccagtggaga acacccaact gcagaacgag
     8881 aagctctacc tgtactacct ccagaacggc agggatatgt acgtggacca agagctggat
     8941 atcaaccgcc tcagcgatta cgacgtcgat catatcgttc cccagtcttt cctgaaggat
     9001 gactccatcg acaacaaggt cctcaccagg tcggacaaga accgcggcaa gtcagataac
     9061 gttccatctg aggaggtcgt taagaagatg aagaactact ggaggcagct cctgaacgcc
     9121 aagctgatca cgcaaaggaa gttcgacaac ctcaccaagg ctgagagagg cgggctctca
     9181 gagctggaca aggccggctt catcaagcgg cagctggtcg agaccagaca aatcacgaag
     9241 cacgttgcgc aaatcctcga ctctcggatg aacacgaagt acgatgagaa cgacaagctg
     9301 atcagggagg ttaaggtgat caccctgaag tctaagctcg tctccgactt caggaaggat
     9361 ttccagttct acaaggttcg cgagatcaac aactaccacc atgcccatga cgcttacctc
     9421 aacgctgtgg tcggcaccgc tctgatcaag aagtacccaa agctggagtc cgagttcgtg
     9481 tacggggact acaaggttta cgatgtgcgc aagatgatcg ccaagtcgga gcaagagatc
     9541 ggcaaggcta ccgccaagta cttcttctac tcaaacatca tgaacttctt caagaccgag
     9601 atcacgctgg ccaacggcga gatccggaag agaccgctca tcgagaccaa cggcgagacg
     9661 ggggagatcg tgtgggacaa gggcagggat ttcgcgaccg tccgcaaggt tctctccatg
     9721 ccccaggtga acatcgtcaa gaagaccgag gtccaaacgg gcgggttctc aaaggagtct
     9781 atcctgccta agcggaacag cgacaagctc atcgccagaa agaaggactg ggacccaaag
     9841 aagtacggcg ggttcgacag ccctaccgtg gcctactcgg tcctggttgt ggcgaaggtt
     9901 gagaagggca agtccaagaa gctcaagagc gtgaaggagc tcctggggat caccatcatg
     9961 gagaggtcca gcttcgagaa gaacccaatc gacttcctgg aggccaaggg ctacaaggag
    10021 gtgaagaagg acctgatcat caagctcccg aagtactctc tcttcgagct ggagaacggc
    10081 aggaagagaa tgctggcttc cgctggcgag ctccagaagg ggaacgagct cgcgctgcca
    10141 agcaagtacg tgaacttcct ctacctggct tcccactacg agaagctcaa gggcagcccg
    10201 gaggacaacg agcaaaagca gctgttcgtc gagcagcaca agcattacct cgacgagatc
    10261 atcgagcaaa tctccgagtt cagcaagcgc gtgatcctcg ccgacgcgaa cctggataag
    10321 gtcctctccg cctacaacaa gcaccgggac aagcccatca gagagcaagc ggagaacatc
    10381 atccatctct tcaccctgac gaacctcggc gctcctgctg ctttcaagta cttcgacacc
    10441 acgatcgatc ggaagagata cacctccacg aaggaggtcc tggacgcgac cctcatccac
    10501 cagtcgatca ccggcctgta cgagacgagg atcgacctct cacaactcgg cggggataag
    10561 agacccgcag caaccaagaa ggcagggcaa gcaaagaaga agaagtgact cgagatatga
    10621 agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt
    10681 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
    10741 tcacattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
    10801 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
    10861 taaggagctc cggtgacgga ctggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    10921 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    10981 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    11041 gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
    11101 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
    11161 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
    11221 cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag
    11281 tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag
    11341 ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc
    11401 gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg
    11461 ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac
    11521 gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca
    11581 ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga
    11641 cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc
    11701 gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca
    11761 cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc
    11821 taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg
    11881 gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg
    11941 aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc
    12001 gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc
    12061 gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg
    12121 aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat
    12181 cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg gctcaaccgt
    12241 gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag
    12301 cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa
    12361 cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa
    12421 ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc
    12481 atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat
    12541 tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc
    12601 gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac
    12661 ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca
    12721 gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac
    12781 ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt
    12841 gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc
    12901 gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact
    12961 gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc
    13021 caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa
    13081 tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg
    13141 caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg
    13201 cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc
    13261 tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga
    13321 tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc
    13381 gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc
    13441 tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca
    13501 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga
    13561 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt
    13621 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc
    13681 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct
    13741 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga
    13801 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg
    13861 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg
    13921 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc
    13981 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa
    14041 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc
    14101 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga
    14161 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc
    14221 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc
    14281 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc
    14341 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca
    14401 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc
    14461 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct
    14521 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag
    14581 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt
    14641 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc
    14701 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt
    14761 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat
    14821 aactgtctgg ccagcgcaca gcccaagagc tgcaaaaagc gcctaccctt cggtcgctgc
    14881 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg
    14941 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc
    15001 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
    15061 tgacacatgc agctcccgga aacggtcaca gcttgtctgt aagcggatgc cgggagcaga
    15121 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    15181 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    15241 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    15301 tcaggccctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    15361 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    15421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    15481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    15541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    15601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    15661 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    15721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    15781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    15841 cagccactgg taacaggatt agcagagcga ggtat
//
