LOCUS       sequence-182411-       16383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant 
            Selection: PvUbi2:hpt II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16383)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 16383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTaCas9-H840A_5
FEATURES             Location/Qualifiers
     source          1..16383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4828..4833,4834..4851,4852..4858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4866..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4871..4893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4890..4906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4890..4906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6962..9478,9479..9481,9482..11065)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             11066..11113
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      11124..11372
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(11394..11411)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11394..11410)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11403..11425)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    11613..11637
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12938..13567
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13996..15069
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15135..15329
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15565..15587)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15673..15813
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15728..15747
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15828..15845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(15999..16383,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16079..16098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac
     2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat
     2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa
     3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa
     3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg
     3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc
     3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta
     3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat
     3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata
     3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca
     3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga
     3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt
     3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca
     3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc
     3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac
     3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca
     3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca
     3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg
     3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc
     3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat
     4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga
     4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt
     4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag
     4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg
     4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt
     4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat
     4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc
     4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt
     4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt
     4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta
     4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt
     4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag
     4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4921 gagctcggta cccggggatc tgcaggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc
     4981 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     5041 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     5101 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     5161 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     5221 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     5281 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     5341 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     5401 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     5461 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     5521 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     5581 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     5641 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     5701 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     5761 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     5821 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     5881 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     5941 cgctcgtcct cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
     6001 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
     6061 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
     6121 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
     6181 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
     6241 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
     6301 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt ctgtttcaaa
     6361 ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta
     6421 cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt
     6481 tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt
     6541 tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta
     6601 tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat
     6661 ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat
     6721 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata
     6781 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca
     6841 gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg
     6901 tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcagggc gatctattcg
     6961 aatggacaag aagtactcga tcggcctcga catcgggacg aactcagttg gctgggccgt
     7021 gatcaccgac gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg
     7081 ccattccatc aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga
     7141 ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg
     7201 ctacctccaa gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg
     7261 cctggaggag tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg
     7321 caacatcgtg gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa
     7381 gaagctcgtg gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca
     7441 catgatcaag ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga
     7501 tgttgacaag ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc
     7561 gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag
     7621 gaggctggag aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa
     7681 cctcatcgct ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga
     7741 ggacgccaag ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc
     7801 ccagatcggc gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat
     7861 cctcctgtca gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc
     7921 catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg
     7981 ccaacagctc ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc
     8041 tgggtacatc gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct
     8101 ggagaagatg gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg
     8161 gaagcagaga accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca
     8221 cgccatcctg agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat
     8281 cgagaagatc ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc
     8341 aagattcgct tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga
     8401 ggtggtggac aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa
     8461 gaacctgccc aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt
     8521 ctacaacgag ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct
     8581 gtccggggag cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac
     8641 ggttaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat
     8701 cagcggcgtt gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat
     8761 catcaaggat aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt
     8821 gctgaccctc acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc
     8881 ccatctcttc gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg
     8941 gaggctgtcc cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct
     9001 cgacttcctg aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga
     9061 cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct
     9121 gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac
     9181 cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt
     9241 catcgagatg gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg
     9301 catgaagcgc atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc
     9361 agtggagaac acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag
     9421 ggatatgtac gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatgc
     9481 tatcgttccc cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc
     9541 ggacaagaac cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa
     9601 gaactactgg aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct
     9661 caccaaggct gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca
     9721 gctggtcgag accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa
     9781 cacgaagtac gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc
     9841 taagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa
     9901 ctaccaccat gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa
     9961 gtacccaaag ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa
    10021 gatgatcgcc aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc
    10081 aaacatcatg aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag
    10141 accgctcatc gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt
    10201 cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt
    10261 ccaaacgggc gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat
    10321 cgccagaaag aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc
    10381 ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt
    10441 gaaggagctc ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga
    10501 cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa
    10561 gtactctctc ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct
    10621 ccagaagggg aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc
    10681 ccactacgag aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga
    10741 gcagcacaag cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt
    10801 gatcctcgcc gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa
    10861 gcccatcaga gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc
    10921 tcctgctgct ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa
    10981 ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat
    11041 cgacctctca caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc
    11101 aaagaagaag aagtgactcg agatatgaag atgaagatga aatatttggt gtgtcaaata
    11161 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
    11221 ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat
    11281 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
    11341 ggtatggact atggaatatg attaaagata aggagctccg gtgacggact ggcactggcc
    11401 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    11461 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    11521 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc
    11581 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
    11641 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
    11701 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
    11761 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
    11821 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
    11881 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
    11941 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
    12001 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
    12061 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
    12121 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
    12181 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
    12241 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
    12301 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
    12361 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
    12421 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
    12481 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
    12541 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
    12601 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
    12661 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
    12721 tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
    12781 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
    12841 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
    12901 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
    12961 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
    13021 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
    13081 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
    13141 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
    13201 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
    13261 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
    13321 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
    13381 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
    13441 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
    13501 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
    13561 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
    13621 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
    13681 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
    13741 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
    13801 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
    13861 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
    13921 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
    13981 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
    14041 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
    14101 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
    14161 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
    14221 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
    14281 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
    14341 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
    14401 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
    14461 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
    14521 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
    14581 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
    14641 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
    14701 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    14761 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    14821 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    14881 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    14941 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    15001 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    15061 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    15121 aaaggcctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    15181 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    15241 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    15301 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc ccaagagctg
    15361 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    15421 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    15481 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    15541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaaa cggtcacagc
    15601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    15661 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
    15721 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    15781 cacagatgcg taaggagaaa ataccgcatc aggccctctt ccgcttcctc gctcactgac
    15841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    15901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    15961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    16021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    16081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    16141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    16201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    16261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    16321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    16381 tat
//
