LOCUS       sequence-182414-       15876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant 
            Selection: PvUbi2:bar.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15876)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 15876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTaCas9-dead_6
FEATURES             Location/Qualifiers
     source          1..15876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    4284..4305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4320..4325,4326..4343,4344..4350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4358..4374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4363..4385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4382..4398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4382..4398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6455..6481,6482..6484,6485..8971,8972..8974,
                     8975..10558)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10559..10606
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      10617..10865
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(10887..10904)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10887..10903)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10896..10918)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    11106..11130
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12431..13060
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13489..14562
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14628..14822
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15058..15080)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15166..15306
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15221..15240
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15321..15338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(15492..15876,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15572..15591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtgga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcataagctt ctgcaaaaga gaaccagaca acagggtaag tgcctagcag taaacaaaca
     2401 gaactcatca caagcaaaca gcaacatcat attcatacca acaggtcatg tgtgttcatc
     2461 acatcattag tactaagcat gccatcatcc aagtatatca aagtaagggc aaagagcatg
     2521 catgatcatc aggtgcacaa aagaatcatc aaattgtagc agtacatatc ttcatctatc
     2581 atgcatatct atccataaca ggacgatgca tgttgaccag gtaaaagcta caggatccta
     2641 taggaacagc agcgtatata tcttcaccaa tcttagcatc ttaatcatgt ggcacatgca
     2701 gtttcaattt aagcacatga gctagttgat tatgaggtac cagagaatca tcaaactgat
     2761 gtagcagcat atatcctcat ctatcatgca atctaatcta atctaactaa acaggaaagg
     2821 tgtgctattc agttaaaagc taccgcatca tatacaaacg gcagcataag aaaaagcata
     2881 atcatcttaa tcatgcaaca aacgcagatt cataataagc ccaagagcta gcttgtgatg
     2941 atcttattct actctgatct acagcaatca gataacgacc taaccttgca catggcaaca
     3001 aaacaatcga tcggacgaat cagttgtttg ttcctagcta gcaccatcga accagataat
     3061 agatgcacgt acagatcccg aaaacgaacc caaaaacagg gcagacctag ctgaacctag
     3121 gcagcgaccc agcagatcgt gagaacgatc tcatctacga acagcctaga agcaacccca
     3181 cgattcccgg acaaacgacc taaaatcccc acaaatcaca tgagcatgac aggataaaca
     3241 gcggaaccga tcagatctac acgaaaaccc cacctcccag ccacccacga tcaggaaaca
     3301 cgcggatcta gcatgatttc gtcaacgcct cagcctagtt cctagccaca gaccaagcag
     3361 aaccaccaaa ccacgccgag cgaggagatg gggcaagagg acgggggaga cgatcgccgt
     3421 accttgaagc gggggaagga tcgccgaggg tcgcgaggag agcaatttgg atttggaacc
     3481 cgggggttgt gcgctccgaa cgatgagacg atgtgagatt gtgggaagag gcgcggaggg
     3541 ccctgtattt atgggctgcg acggggggag gagaggtggg gagggttggg gaaggaatcc
     3601 cccacccgtg ccgtgacggt tccgggccgt gtgagaggag cccgctcgtc tccgccacgc
     3661 aatttccgcg atcggagcgg agctttcgag aggcggctgg atggttggtg gccgttagat
     3721 ttgtagacgc cgttaacgcc tcgcctccac cgggaagagt tttgagcagc cgcttatgac
     3781 aatggcttaa cgacgttaga cggagcgtta gtggcaggcc atgatagaat agacgtattg
     3841 caatgggatt ataattaaat aaataagaat ataataagat atggcaagtc ggcactcatg
     3901 acatggtctt cgaaatgata gtgctcactt tcttagccga gaaagttgac gcgactgatt
     3961 tagaagttaa gatattattt ctctcttctt ttctttcctc gtcatataag gatgaaataa
     4021 actttagaga ttgccggtag tgattttgga tttcggcgat caggcttggt ttgccggttt
     4081 cggacggtgt gccttaggcc acccgcagtg tatcttgtaa tgttcaaccg ataagcaagg
     4141 gtggggctca agcaagtagt aaacaactat gtcaaatgtc accatggtta tggtcttgtt
     4201 tagttggctt cttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     4321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     4381 acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga tctgcaggcg
     4441 cgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag agataatgag cattgcatgt
     4501 ctaagttata aaaaattacc acatattttt tttgtcacac ttgtttgaag tgcagtttat
     4561 ctatctttat acatatattt aaactttact ctacgaataa tataatctat agtactacaa
     4621 taatatcagt gttttagaga atcatataaa tgaacagtta gacatggtct aaaggacaat
     4681 tgagtatttt gacaacagga ctctacagtt ttatcttttt agtgtgcatg tgttctcctt
     4741 tttttttgca aatagcttca cctatataat acttcatcca ttttattagt acatccattt
     4801 agggtttagg gttaatggtt tttatagact aattttttta gtacatctat tttattctat
     4861 tttagcctct aaattaagaa aactaaaact ctattttagt ttttttattt aataatttag
     4921 atataaaata gaataaaata aagtgactaa aaattaaaca aatacccttt aagaaattaa
     4981 aaaaactaag gaaacatttt tcttgtttcg agtagataat gccagcctgt taaacgccgt
     5041 cgacgagtct aacggacacc aaccagcgaa ccagcagcgt cgcgtcgggc caagcgaagc
     5101 agacggcacg gcatctctgt cgctgcctct ggacccctct cgagagttcc gctccaccgt
     5161 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
     5221 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
     5281 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
     5341 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
     5401 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc cccctctcta
     5461 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     5521 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     5581 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     5641 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     5701 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     5761 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     5821 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     5881 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     5941 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     6001 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     6061 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     6121 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     6181 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     6241 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     6301 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     6361 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     6421 tttggtgtta cttcctgcag ggcgatctat tcgaatggac aagaagtact cgatcggcct
     6481 cgccatcggg acgaactcag ttggctgggc cgtgatcacc gacgagtaca aggtgccctc
     6541 taagaagttc aaggtcctgg ggaacaccga ccgccattcc atcaagaaga acctcatcgg
     6601 cgctctcctg ttcgacagcg gggagaccgc tgaggctacg aggctcaaga gaaccgctag
     6661 gcgccggtac acgagaagga agaacaggat ctgctacctc caagagattt tctccaacga
     6721 gatggccaag gttgacgatt cattcttcca ccgcctggag gagtctttcc tcgtggagga
     6781 ggataagaag cacgagcggc atcccatctt cggcaacatc gtggacgagg ttgcctacca
     6841 cgagaagtac cctacgatct accatctgcg gaagaagctc gtggactcca ccgataaggc
     6901 ggacctcaga ctgatctacc tcgctctggc ccacatgatc aagttccgcg gccatttcct
     6961 gatcgagggg gatctcaacc cagacaacag cgatgttgac aagctgttca tccaactcgt
     7021 gcagacctac aaccaactct tcgaggagaa cccgatcaac gcctctggcg tggacgcgaa
     7081 ggctatcctg tccgcgaggc tctcgaagtc caggaggctg gagaacctga tcgctcagct
     7141 cccaggcgag aagaagaacg gcctgttcgg gaacctcatc gctctcagcc tggggctcac
     7201 cccgaacttc aagtcgaact tcgatctcgc tgaggacgcc aagctgcaac tctccaagga
     7261 cacctacgac gatgacctcg ataacctcct ggcccagatc ggcgatcaat acgcggacct
     7321 gttcctcgct gccaagaacc tgtcggacgc catcctcctg tcagatatcc tccgcgtgaa
     7381 caccgagatc acgaaggctc cactctctgc ctccatgatc aagcgctacg acgagcacca
     7441 tcaggatctg accctcctga aggcgctggt ccgccaacag ctcccggaga agtacaagga
     7501 gattttcttc gatcagtcga agaacggcta cgctgggtac atcgacggcg gggcctcaca
     7561 agaggagttc tacaagttca tcaagccaat cctggagaag atggacggca cggaggagct
     7621 cctggtgaag ctcaacaggg aggacctcct gcggaagcag agaaccttcg ataacggcag
     7681 catcccccac caaatccatc tcggggagct gcacgccatc ctgagaaggc aagaggactt
     7741 ctaccctttc ctcaaggata accgggagaa gatcgagaag atcctgacct tcagaatccc
     7801 atactacgtc ggccctctcg cgcgggggaa ctcaagattc gcttggatga cccgcaagtc
     7861 tgaggagacc atcacgccgt ggaacttcga ggaggtggtg gacaagggcg ctagcgctca
     7921 gtcgttcatc gagaggatga ccaacttcga caagaacctg cccaacgaga aggtgctccc
     7981 taagcactcg ctcctgtacg agtacttcac cgtctacaac gagctcacga aggtgaagta
     8041 cgtcaccgag ggcatgcgca agccagcgtt cctgtccggg gagcagaaga aggctatcgt
     8101 ggacctcctg ttcaagacca accggaaggt cacggttaag caactcaagg aggactactt
     8161 caagaagatc gagtgcttcg attcggtcga gatcagcggc gttgaggacc gcttcaacgc
     8221 cagcctcggg acctaccacg atctcctgaa gatcatcaag gataaggact tcctggacaa
     8281 cgaggagaac gaggatatcc tggaggacat cgtgctgacc ctcacgctgt tcgaggacag
     8341 ggagatgatc gaggagcgcc tgaagacgta cgcccatctc ttcgatgaca aggtcatgaa
     8401 gcaactcaag cgccggagat acaccggctg ggggaggctg tcccgcaagc tcatcaacgg
     8461 catccgggac aagcagtccg ggaagaccat cctcgacttc ctgaagagcg atggcttcgc
     8521 caacaggaac ttcatgcaac tgatccacga tgacagcctc accttcaagg aggatatcca
     8581 aaaggctcaa gtgagcggcc agggggactc gctgcacgag catatcgcga acctcgctgg
     8641 ctcccccgcg atcaagaagg gcatcctcca gaccgtgaag gttgtggacg agctcgtgaa
     8701 ggtcatgggc cggcacaagc ctgagaacat cgtcatcgag atggccagag agaaccaaac
     8761 cacgcagaag gggcaaaaga actctaggga gcgcatgaag cgcatcgagg agggcatcaa
     8821 ggagctgggg tcccaaatcc tcaaggagca cccagtggag aacacccaac tgcagaacga
     8881 gaagctctac ctgtactacc tccagaacgg cagggatatg tacgtggacc aagagctgga
     8941 tatcaaccgc ctcagcgatt acgacgtcga tgctatcgtt ccccagtctt tcctgaagga
     9001 tgactccatc gacaacaagg tcctcaccag gtcggacaag aaccgcggca agtcagataa
     9061 cgttccatct gaggaggtcg ttaagaagat gaagaactac tggaggcagc tcctgaacgc
     9121 caagctgatc acgcaaagga agttcgacaa cctcaccaag gctgagagag gcgggctctc
     9181 agagctggac aaggccggct tcatcaagcg gcagctggtc gagaccagac aaatcacgaa
     9241 gcacgttgcg caaatcctcg actctcggat gaacacgaag tacgatgaga acgacaagct
     9301 gatcagggag gttaaggtga tcaccctgaa gtctaagctc gtctccgact tcaggaagga
     9361 tttccagttc tacaaggttc gcgagatcaa caactaccac catgcccatg acgcttacct
     9421 caacgctgtg gtcggcaccg ctctgatcaa gaagtaccca aagctggagt ccgagttcgt
     9481 gtacggggac tacaaggttt acgatgtgcg caagatgatc gccaagtcgg agcaagagat
     9541 cggcaaggct accgccaagt acttcttcta ctcaaacatc atgaacttct tcaagaccga
     9601 gatcacgctg gccaacggcg agatccggaa gagaccgctc atcgagacca acggcgagac
     9661 gggggagatc gtgtgggaca agggcaggga tttcgcgacc gtccgcaagg ttctctccat
     9721 gccccaggtg aacatcgtca agaagaccga ggtccaaacg ggcgggttct caaaggagtc
     9781 tatcctgcct aagcggaaca gcgacaagct catcgccaga aagaaggact gggacccaaa
     9841 gaagtacggc gggttcgaca gccctaccgt ggcctactcg gtcctggttg tggcgaaggt
     9901 tgagaagggc aagtccaaga agctcaagag cgtgaaggag ctcctgggga tcaccatcat
     9961 ggagaggtcc agcttcgaga agaacccaat cgacttcctg gaggccaagg gctacaagga
    10021 ggtgaagaag gacctgatca tcaagctccc gaagtactct ctcttcgagc tggagaacgg
    10081 caggaagaga atgctggctt ccgctggcga gctccagaag gggaacgagc tcgcgctgcc
    10141 aagcaagtac gtgaacttcc tctacctggc ttcccactac gagaagctca agggcagccc
    10201 ggaggacaac gagcaaaagc agctgttcgt cgagcagcac aagcattacc tcgacgagat
    10261 catcgagcaa atctccgagt tcagcaagcg cgtgatcctc gccgacgcga acctggataa
    10321 ggtcctctcc gcctacaaca agcaccggga caagcccatc agagagcaag cggagaacat
    10381 catccatctc ttcaccctga cgaacctcgg cgctcctgct gctttcaagt acttcgacac
    10441 cacgatcgat cggaagagat acacctccac gaaggaggtc ctggacgcga ccctcatcca
    10501 ccagtcgatc accggcctgt acgagacgag gatcgacctc tcacaactcg gcggggataa
    10561 gagacccgca gcaaccaaga aggcagggca agcaaagaag aagaagtgac tcgagatatg
    10621 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
    10681 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
    10741 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
    10801 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
    10861 ataaggagct ccggtgacgg actggcactg gccgtcgttt tacaacgtcg tgactgggaa
    10921 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    10981 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    11041 tgctagagca gcttgagctt ggatcagatt gtcgtttccc gccttcagtt taaactatca
    11101 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
    11161 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
    11221 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
    11281 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
    11341 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
    11401 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
    11461 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
    11521 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
    11581 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
    11641 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
    11701 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
    11761 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
    11821 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
    11881 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
    11941 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
    12001 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
    12061 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
    12121 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
    12181 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ggctcaaccg
    12241 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
    12301 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
    12361 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
    12421 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
    12481 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
    12541 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
    12601 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
    12661 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
    12721 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
    12781 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
    12841 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
    12901 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
    12961 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
    13021 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
    13081 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
    13141 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
    13201 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
    13261 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
    13321 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
    13381 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
    13441 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
    13501 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
    13561 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
    13621 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
    13681 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
    13741 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
    13801 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
    13861 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
    13921 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
    13981 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
    14041 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
    14101 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
    14161 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
    14221 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
    14281 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
    14341 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
    14401 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
    14461 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
    14521 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
    14581 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggcc tctttcctgt ggatagcacg
    14641 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
    14701 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
    14761 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
    14821 taactgtctg gccagcgcac agcccaagag ctgcaaaaag cgcctaccct tcggtcgctg
    14881 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
    14941 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
    15001 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
    15061 ctgacacatg cagctcccgg aaacggtcac agcttgtctg taagcggatg ccgggagcag
    15121 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
    15181 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
    15241 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    15301 atcaggccct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    15361 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    15421 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    15481 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    15541 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    15601 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    15661 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    15721 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    15781 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    15841 gcagccactg gtaacaggat tagcagagcg aggtat
//
