LOCUS       sequence-182416-        2768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of wildtype Rag GTPase dimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Shen K, Choe A, Sabatini DM
  TITLE     Intersubunit Crosstalk in the Rag GTPase Heterodimer Enables mTORC1 
            to Respond Rapidly to Amino Acid Availability.
  JOURNAL   Mol Cell. 2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 
            10.1016/j.molcel.2017.09.026.
  PUBMED    29056322
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2017 Oct 17. pii: S1097-2765(17)30704-9. doi: 
            10.1016/j.molcel.2017.09.026."
COMMENT     Sequence Label: pK RagA-RagC
FEATURES             Location/Qualifiers
     source          1..2768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..48
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             79..369
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG"
     CDS             406..429
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     1443..1462
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1443..1461
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1462..1486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2735..2752
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tataccatgg gcagcagcca tcaccatcac catcaccatc accatcatag ccgccgccgt
       61 cgccgccgtc gcccgcggat gtcggactca gaagtcaatc aagaagctaa gccagaggtc
      121 aagccagaag tcaagcctga gactcacatc aatttaaagg tgtccgatgg atcttcagag
      181 atcttcttca agatcaaaaa gaccactcct ttaagaaggc tgatggaagc gttcgctaaa
      241 agacagggta aggaaatgga ctccttaaga ttcttgtacg acggtattag aattcaagct
      301 gatcagaccc ctgaagattt ggacatggag gataacgata ttattgaggc tcacagagaa
      361 cagattggtc cgcggggtgg tggcgaccat cctccaaaat cggatctgga agttctgttc
      421 caggggcccg gatccatgcc gaacaccgct atgaaaaaaa aagtgctgct gatgggtaaa
      481 tcgggctcgg gtaaaacctc aatgcgtagt attatctttg cgaactatat tgcgcgtgat
      541 acccgtcgcc tgggtgcaac gatcgacgtg gaacatagcc acgttcgctt tctgggtaac
      601 ctggtgctga atctgtggga ttgcggcggt caggacacct ttatggaaaa ttatttcacg
      661 tcgcaacgtg ataacatttt tcgcaatgtc gaagtgctga tctacgtttt cgatgtcgaa
      721 tcccgtgaac tggaaaaaga catgcattat taccagtcct gcctggaagc aattctgcaa
      781 aactcaccgg atgctaaaat cttttgtctg gtccacaaaa tggacctggt gcaggaagat
      841 caacgtgacc tgattttcaa agaacgcgaa gaagatctgc gtcgcctgtc gcgtccgctg
      901 gaatgcgcgt gttttcgcac cagcatctgg gacgaaacgc tgtataaagc ctggagctct
      961 attgtttacc agctgatccc gaatgtccag caactggaaa tgaacctgcg taatttcgca
     1021 cagattatcg aagctgatga agtgctgctg tttgaacgcg caaccttcct ggttatttcc
     1081 cattatcagt gcaaagaaca acgtgatgtg caccgcttcg aaaaaatctc aaacatcatc
     1141 aaacagttca aactgagttg ttccaaactg gcggcctcat ttcagtcgat ggaagtccgc
     1201 aactctaatt ttgcagcttt cattgatatc ttcaccagta atacgtacgt tatggtggtt
     1261 atgagcgacc cgtctattcc gagtgcggcc accctgatta acatccgtaa tgcccgcaaa
     1321 cattttgaaa aactggaacg tgttgatggc ccgaaacaca gcctgctgat gcgctgagcg
     1381 gccgcataat gcttaagtcg aacagaaagt aatcgtattg tacacggccg cataatcgaa
     1441 attaatacga ctcactatag gggaattgtg agcggataac aattccccat cttagtatat
     1501 tagttaagta taagaaggag atatacatat gtcactgcaa tacggtgctg aagaaacgcc
     1561 gctggctggt agttatggtg ctgctgattc attcccgaaa gattttggtt atggcgttga
     1621 agaagaagaa gaagaagcgg cggcggcggg cggcggcgtg ggtgcaggtg ccggtggcgg
     1681 ttgtggcccg ggcggtgccg atagctctaa accgcgtatt ctgctgatgg gcctgcgtcg
     1741 cagtggtaaa agttccatcc agaaagtggt ttttcacaaa atgtcgccga acgaaaccct
     1801 gttcctggaa agcacgaaca aaatctacaa agatgacatc agcaactcat cgtttgtgaa
     1861 tttccagatt tgggattttc cgggtcaaat ggatttcttt gacccgacct ttgactacga
     1921 aatgatcttc cgtggcacgg gtgcgctgat ttatgttatc gatgcccaag atgactacat
     1981 ggaagcactg acccgcctgc atattacggt gtcaaaagcg tataaagtta acccggatat
     2041 gaacttcgaa gttttcatcc ataaagtgga tggcctgtcg gatgaccaca aaattgaaac
     2101 ccagcgtgat atccatcaac gcgcaaacga tgacctggca gacgctggtc tggaaaaact
     2161 gcacctgagt ttttatctga ccagcattta cgatcattca atctttgaag cgttcagcaa
     2221 agtcgtgcag aaactgattc cgcaactgcc gacgctggaa aacctgctga acatcttcat
     2281 cagtaactcc ggcatcgaaa aagcctttct gttcgatgtt gtctctaaaa tctacatcgc
     2341 aaccgatagc tctccggtgg acatgcagag ttacgaactg tgctgtgata tgattgacgt
     2401 ggttatcgat gtttcctgca tttatggtct gaaagaagat ggctcaggtt cggcttacga
     2461 caaagaatca atggcgatta tcaaactgaa caataccacg gtcctgtatc tgaaagaagt
     2521 gaccaaattt ctggctctgg tgtgcattct gcgtgaagaa tctttcgaac gcaaaggcct
     2581 gatcgattac aacttccatt gtttccgtaa agccatccac gaagtttttg aagtcggcgt
     2641 gacgagccat cgctcttgcg gtcaccaaac gagcgcaagc agcctgaaag ccctgaccca
     2701 caatggcacc ccgcgtaatg caatctgact cgagcaccac caccaccacc actgagatcc
     2761 ggctgcta
//
