LOCUS       sequence-18242-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Cave JW, Cho HS, Batchelder AM, Yokota H, Kim R, Wemmer DE
  TITLE     Solution nuclear magnetic resonance structure of a protein disulfide
            oxidoreductase from Methanococcus jannaschii.
  JOURNAL   Protein Sci. 2001 Feb . 10(2):384-96.
  PUBMED    11266624
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2001 Feb . 10(2):384-96."
COMMENT     Sequence Label: pET21a.MJ0307
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(322..338)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(322..338)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(375..395)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(375..393)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(414..430)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(414..430)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(427..449)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    438..454
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(462..468,469..486,487..492))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    507..528
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(645..662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgacggtatc gatagcttga tatcgaattc ctgcagccca aggatccata tgtcaaaggt
       61 aaagatagag ctttttacat caccaatgtg tcctcactgt cctgcagcta aaagagttgt
      121 tgaagaggta gcaaatgaaa tgccggatgc tgttgaagta gaatacataa acgttatgga
      181 gaatcctcaa aaggcaatgg aatatgggat aatggcagtt ccaacaattg taataaatgg
      241 ggatgttgag tttattggag ctcctacaaa ggaggcatta gttgaggcaa tcaaaaaaag
      301 actatgactc gagggatggg ggatccacta gttctagagc ggccgccacc gcggtggagc
      361 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
      421 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      481 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      541 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      601 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      841 gagcatcaca aaaatcgacg ctagtca
//
