LOCUS       sequence-182420-       18606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A 
            double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18606)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 18606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pJG382
FEATURES             Location/Qualifiers
     source          1..18606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1629..2423)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2848..2872
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        3813..4158
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4101..4121
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(4192..4218,4219..4221,4222..8295)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8308..8328
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8339..8587
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        9076..9151
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        9509..9584
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             11068..11859
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11119..11138)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11729..11748
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      11886..12138
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13836..13860
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15161..15790
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             16219..17292
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      17358..17552
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(17788..17810)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17896..18036
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17951..17970
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18051..18068)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(18222..18606,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18302..18321)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggctg atgaatcccc taatgatttt tatcaaaatc
      301 attaagttaa ggtagataca catcttgtca tatgatcaaa tggtttcgcc aaaaatcaat
      361 aatcagacaa caaaatgtgc gaactcgata ttttacacga ctctctttac caattctgcc
      421 ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgcc ttacttgact
      481 gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac
      541 ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg
      601 ctgtataccg ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg
      661 ttgactggtc tgatatccgt gagcaaaaac ggcttatggt attgcgagct tcagtcgcac
      721 tacacggtcg ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa
      781 agaaagctca tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac
      841 cgctcattgt cagtgatgct ggctttaaag tgccatggta taaatccgtt gagaagctgg
      901 gttggtactg gttaagtcga gtaagaggaa aagtacaata tgcagaccta ggagcggaaa
      961 actggaaacc tatcagcaac ttacatgata tgtcatctag tcactcaaag actttaggct
     1021 ataagaggct gactaaaagc aatccaatct catgccaaat tctattgtat aaatctcgct
     1081 ctaaaggccg aaaaaatcag cgctcgacac ggactcattg tcaccacccg tcacctaaaa
     1141 tctactcagc gtcggcaaag gagccatgga ttctagcaac taacttacct gttgaaattc
     1201 gaacacccaa acaacttgtt aatatctatt cgaagcgaat gcagattgaa gaaaccttcc
     1261 gagacttgaa aagtcctgcc tacggactag gcctacgcca tagccgaacg agcagctcag
     1321 agcgttttga tatcatgctg ctaatcgccc tgatgcttca actaacatgt tggcttgcgg
     1381 gcgttcatgc tcagaaacaa ggttgggaca agcacttcca ggctaacaca gtcagaaatc
     1441 gaaacgtact ctcaacagtt cgcttaggca tggaagtttt gcggcattct ggctacacaa
     1501 taacaaggga agacttactc gtggctgcaa ccctactagc tcaaaattta ttcacacatg
     1561 gttacgcttt ggggaaatta tgaggggatc tctcagcgtt aagggatttt ggtcatgcat
     1621 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     1681 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
     1741 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
     1801 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
     1861 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
     1921 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
     1981 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
     2041 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
     2101 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
     2161 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
     2221 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
     2281 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
     2341 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
     2401 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     2461 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     2521 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     2581 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     2641 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     2701 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     2761 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     2821 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     2881 gcttagacaa cttaataaca cattgcggac gtttttaatg tagagctcaa agtttaacgc
     2941 gttagcagaa ggcatgttgt tgtgactccg aggggttgcc tcaaactcta tcttataacc
     3001 ggcgtggagg catggaggca ggggtatttt ggtcatttta atagatagtg gaaaatgacg
     3061 tggaatttac ttaaagacga agtctttgcg acaagggggg gcccacgccg aatttaatat
     3121 taccggcgtg gccccccctt atcgcgagtg ctttagcacg agcggtccag atttaaagta
     3181 gaaaatttcc cgcccactag ggttaaaggt gttcacacta taaaagcata tacgatgtga
     3241 tggtatttga tggagcgtat attgtatcag gtatttccgt tggatacgaa ttattcgtac
     3301 gaccctcggt accgatcggc gcgccagatt tgccttttca atttcagaaa gaatgctaac
     3361 ccacagatgg ttagagaggc ttacgcagca ggtatcatca agacgatcta cccgagcaat
     3421 aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg
     3481 actaactgca tcaagaacac agagaaagat atatttctca agatcagaag tactattcca
     3541 gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct
     3601 aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta
     3661 acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac
     3721 aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
     3781 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
     3841 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
     3901 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
     3961 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
     4021 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
     4081 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     4141 tttcatttgg agagaacacg ggggactcct gcaggtagat cgctcgtcga catggataag
     4201 aagtactcta tcggactcgc tatcggaact aactctgtgg gatgggctgt gatcaccgat
     4261 gagtacaagg tgccatctaa gaagttcaag gttctcggaa acaccgatag gcactctatc
     4321 aagaaaaacc ttatcggtgc tctcctcttc gattctggtg aaactgctga ggctaccaga
     4381 ctcaagagaa ccgctagaag aaggtacacc agaagaaaga acaggatctg ctacctccaa
     4441 gagatcttct ctaacgagat ggctaaagtg gatgattcat tcttccacag gctcgaagag
     4501 tcattcctcg tggaagaaga taagaagcac gagaggcacc ctatcttcgg aaacatcgtt
     4561 gatgaggtgg cataccacga gaagtaccct actatctacc acctcagaaa gaagctcgtt
     4621 gattctactg ataaggctga tctcaggctc atctacctcg ctctcgctca catgatcaag
     4681 ttcagaggac acttcctcat cgagggtgat ctcaaccctg ataactctga tgtggataag
     4741 ttgttcatcc agctcgtgca gacctacaac cagcttttcg aagagaaccc tatcaacgct
     4801 tcaggtgtgg atgctaaggc tatcctctct gctaggctct ctaagtcaag aaggcttgag
     4861 aacctcattg ctcagctccc tggtgagaag aagaacggac ttttcggaaa cttgatcgct
     4921 ctctctctcg gactcacccc taacttcaag tctaacttcg atctcgctga ggatgcaaag
     4981 ctccagctct caaaggatac ctacgatgat gatctcgata acctcctcgc tcagatcgga
     5041 gatcagtacg ctgatttgtt cctcgctgct aagaacctct ctgatgctat cctcctcagt
     5101 gatatcctca gagtgaacac cgagatcacc aaggctccac tctcagcttc tatgatcaag
     5161 agatacgatg agcaccacca ggatctcaca cttctcaagg ctcttgttag acagcagctc
     5221 ccagagaagt acaaagagat tttcttcgat cagtctaaga acggatacgc tggttacatc
     5281 gatggtggtg catctcaaga agagttctac aagttcatca agcctatcct cgagaagatg
     5341 gatggaaccg aggaactcct cgtgaagctc aatagagagg atcttctcag aaagcagagg
     5401 accttcgata acggatctat ccctcatcag atccacctcg gagagttgca cgctatcctt
     5461 agaaggcaag aggatttcta cccattcctc aaggataaca gggaaaagat tgagaagatt
     5521 ctcaccttca gaatccctta ctacgtggga cctctcgcta gaggaaactc aagattcgct
     5581 tggatgacca gaaagtctga ggaaaccatc accccttgga acttcgaaga ggtggtggat
     5641 aagggtgcta gtgctcagtc tttcatcgag aggatgacca acttcgataa gaaccttcca
     5701 aacgagaagg tgctccctaa gcactctttg ctctacgagt acttcaccgt gtacaacgag
     5761 ttgaccaagg ttaagtacgt gaccgaggga atgaggaagc ctgctttttt gtcaggtgag
     5821 caaaagaagg ctatcgttga tctcttgttc aagaccaaca gaaaggtgac cgtgaagcag
     5881 ctcaaagagg attacttcaa gaaaatcgag tgcttcgatt cagttgagat ttctggtgtt
     5941 gaggataggt tcaacgcatc tctcggaacc taccacgatc tcctcaagat cattaaggat
     6001 aaggatttct tggataacga ggaaaacgag gatatcttgg aggatatcgt tcttaccctc
     6061 accctctttg aagatagaga gatgattgaa gaaaggctca agacctacgc tcatctcttc
     6121 gatgataagg tgatgaagca gttgaagaga agaagataca ctggttgggg aaggctctca
     6181 agaaagctca ttaacggaat cagggataag cagtctggaa agacaatcct tgatttcctc
     6241 aagtctgatg gattcgctaa cagaaacttc atgcagctca tccacgatga ttctctcacc
     6301 tttaaagagg atatccagaa ggctcaggtt tcaggacagg gtgatagtct ccatgagcat
     6361 atcgctaacc tcgctggatc tcctgcaatc aagaagggaa tcctccagac tgtgaaggtt
     6421 gtggatgagt tggtgaaggt gatgggaagg cataagcctg agaacatcgt gatcgaaatg
     6481 gctagagaga accagaccac tcagaaggga cagaagaact ctagggaaag gatgaagagg
     6541 atcgaggaag gtatcaaaga gcttggatct cagatcctca aagagcaccc tgttgagaac
     6601 actcagctcc agaatgagaa gctctacctc tactacctcc agaacggaag ggatatgtat
     6661 gtggatcaag agttggatat caacaggctc tctgattacg atgttgatca tatcgtgcca
     6721 cagtcattct tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc tgataagaac
     6781 aggggtaaga gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa gaactattgg
     6841 aggcagctcc tcaacgctaa gctcatcact cagagaaagt tcgataactt gactaaggct
     6901 gagaggggag gactctctga attggataag gcaggattca tcaagaggca gcttgtggaa
     6961 accaggcaga tcactaagca cgttgcacag atcctcgatt ctaggatgaa caccaagtac
     7021 gatgagaacg ataagttgat cagggaagtg aaggttatca ccctcaagtc aaagctcgtg
     7081 tctgatttca gaaaggattt ccaattctac aaggtgaggg aaatcaacaa ctaccaccac
     7141 gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa gtatcctaag
     7201 ctcgagtcag agttcgtgta cggtgattac aaggtgtacg atgtgaggaa gatgatcgct
     7261 aagtctgagc aagagatcgg aaaggctacc gctaagtatt tcttctactc taacatcatg
     7321 aatttcttca agaccgagat taccctcgct aacggtgaga tcagaaagag gccactcatc
     7381 gagacaaacg gtgaaacagg tgagatcgtg tgggataagg gaagggattt cgctaccgtt
     7441 agaaaggtgc tctctatgcc acaggtgaac atcgttaaga aaaccgaggt gcagaccggt
     7501 ggattctcta aagagtctat cctccctaag aggaactctg ataagctcat tgctaggaag
     7561 aaggattggg accctaagaa atacggtggt ttcgattctc ctaccgtggc ttactctgtt
     7621 ctcgttgtgg ctaaggttga gaagggaaag agtaagaagc tcaagtctgt taaggaactt
     7681 ctcggaatca ctatcatgga aaggtcatct ttcgagaaga acccaatcga tttcctcgag
     7741 gctaagggat acaaagaggt taagaaggat ctcatcatca agctcccaaa gtactcactc
     7801 ttcgaactcg agaacggtag aaagaggatg ctcgcttctg ctggtgagct tcaaaaggga
     7861 aacgagcttg ctctcccatc taagtacgtt aactttcttt acctcgcttc tcactacgag
     7921 aagttgaagg gatctccaga agataacgag cagaagcaac ttttcgttga gcagcacaag
     7981 cactacttgg atgagatcat cgagcagatc tctgagttct ctaaaagggt gatcctcgct
     8041 gatgcaaacc tcgataaggt gttgtctgct tacaacaagc acagagataa gcctatcagg
     8101 gaacaggcag agaacatcat ccatctcttc acccttacca acctcggtgc tcctgctgct
     8161 ttcaagtact tcgatacaac catcgatagg aagagataca cctctaccaa agaagtgctc
     8221 gatgctaccc tcatccatca gtctatcact ggactctacg agactaggat cgatctctca
     8281 cagctcggtg gtgattcaag ggctgatcct aagaagaaga ggaaggtttg actcgagata
     8341 tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg
     8401 tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta
     8461 agatcacatt ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga
     8521 tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa
     8581 agataaggag ctccggtgac ggacccatgg cttcgttgaa caacggaaac tcgacttgcc
     8641 ttccgcacaa tacatcattt cttcttagct ttttttcttc ttcttcgttc atacagtttt
     8701 tttttgttta tcagcttaca ttttcttgaa ccgtagcttt cgttttcttc tttttaactt
     8761 tccattcgga gtttttgtat cttgtttcat agtttgtccc aggattagaa tgattaggca
     8821 tcgaaccttc aagaatttga ttgaataaaa catcttcatt cttaagatat gaagataatc
     8881 ttcaaaaggc ccctgggaat ctgaaagaag agaagcaggc ccatttatat gggaaagaac
     8941 aatagtattt cttatatagg cccatttaag ttgaaaacaa tcttcaaaag tcccacatcg
     9001 cttagataag aaaacgaagc tgagtttata tacagctaga gtcgaagtag tgattgcgtc
     9061 ccgggtcgct accttgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
     9121 tcaacttgaa aaagtggcac cgagtcggtg ctttttttcc cggcgccatg gatgttgttg
     9181 ttaccagaaa gtaaataaat gttcaatctc tgatgttctc aagtaagtga gttttattgg
     9241 gaataatatt aacttatgtt cttcttgcat ttgatttctt tgccgctctc ttcttctatc
     9301 ttaaatctgt gtatactatt tcactattgg gctttttatt agtctataat gggactcaaa
     9361 ataaggcttt ggcccacatc aaaaagataa gtcacaaatc aaaactaaat tcagagtctt
     9421 ttctcccaca tcggtcactg tactcatttt gtgtttgttt atatattaca cgaaccgatc
     9481 tttggtacga cgcccacgca ttaaagcggt tttagagcta gaaatagcaa gttaaaataa
     9541 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tcgcgcgtag
     9601 tcctcggtac agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta
     9661 atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg
     9721 caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt
     9781 gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag cgggactttg
     9841 caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
     9901 acagccaaaa gccagacaga gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg
     9961 gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt
    10021 cgtcatgaag atgcggactt gcgtggcaaa ggattcgata acgtgctgat ggtgcacgac
    10081 cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct
    10141 gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct
    10201 gtcggcttta acctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
    10261 tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc gattaaagag
    10321 ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
    10381 gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa
    10441 ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat
    10501 accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa
    10561 agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag
    10621 aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac
    10681 tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
    10741 cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt
    10801 gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac
    10861 cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt
    10921 gaaaaaccgc agcagggagg caaacaacgc agggaggcaa acaatgatat cacaactctc
    10981 ctgacgcgtc atcgtcggct acagcctcgg gaattgctac ctagctcgag caagatccaa
    11041 ggagatataa caatggcttc ctcctggatt gaacaagatg gattgcacgc aggttctccg
    11101 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
    11161 gatgccgccg tgttccggct gtcagcgcag ggtagaccgg ttctttttgt caagaccgac
    11221 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg
    11281 acgggcgtac cttgcgctgc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
    11341 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
    11401 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    11461 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    11521 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    11581 aggctcaagg cgagaatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
    11641 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    11701 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    11761 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    11821 cgcatcgcct tctatcgcct tcttgacgag ttcttctgat aaccgcggag agctcgaatt
    11881 tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc
    11941 ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt
    12001 aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt
    12061 aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt
    12121 catctatgtt actagatcgg agtgtacttc aagtcacacc ggcgagtgtt tgatcgccgg
    12181 cggtaccgag tgtacttcaa gtcagtggga aatcaataaa atgattattt tatgaatata
    12241 tttcattgtg caagtagata gaaattacat atgttacata acacacgaaa taaacaaaaa
    12301 aagacaatcc aaaaacaaac accccaaaaa aaataatcac tttagataaa ctcgtatgag
    12361 gagaggcacg ttcagtgact cgacgattcc cgagcaaaaa aagtctcccc gtcacacatg
    12421 tagtgggtga cgcaattatc tttaaagtaa tccttctgtt gacttgtcat tgataacatc
    12481 cagtcttcgt caggattgca aagaattata gaagggatcc caccttttat tttcttcttt
    12541 tttccatatt tagggttgac agtgaaatca gactggcaac ctattaattg cttccacaat
    12601 gggacgaact tgaaggggat gtcgtcgatg atattatagg tggcgtgttc atcgtagttg
    12661 gtgaaatcga tggtaccgtt ccaatagttg tgtcgtccga gacttctagc ccaggtggtc
    12721 tttccggtac gagttggtcc gcagatgtag aggctggggt gtcggattcc attccttcca
    12781 ttgtccttgt taaatcggcc atccattcaa ggtcagattg agcttgttgg tatgagacag
    12841 gatgtatgta agtataagcg tctatgctta catggtatag atgggtttcc ctccaggagt
    12901 gtagatcttc gtggcagcga agatctgatt ctgtgaaggg cgacacatac ggttcaggtt
    12961 gtggagggaa taatttgttg gctgaatatt ccagccattg aagctttgtt gcccattcat
    13021 gagggaattc ttccttgatc atgtcaagat attcctcctt agacgttgca gtctggataa
    13081 tagttctcca tcgtgcgtca gatttgcgag gagaaacctt atgatctcgg aaatctcctc
    13141 tggttttaat atctccgtcc tttgatatgt aatcaaggac ttgtttagag tttctagctg
    13201 gctggatatt agggtgattt ccttcaaaat cgaaaaaaga aggatcccta atacaaggtt
    13261 ttttatcaag ctggagaaga gcatgatagt gggtagtgcc atcttgatga agctcagaag
    13321 caacaccaag gaagaaaata agaaaaggtg tgagtttctc ccagagaaac tggaataaat
    13381 catctctttg agatgagcac ttgggatagg taaggaaaac atatttagat tggagtctga
    13441 agttcttact agcagaaggc atgttgttgt gactccgagg ggttgcctca aactctatct
    13501 tataaccggc gtggaggcat ggaggcaggg gtattttggt cattttaata gatagtggaa
    13561 aatgacgtgg aatttactta aagacgaagt ctttgcgaca agggggggcc cacgccgaat
    13621 ttaatattac cggcgtggcc cccccttatc gcgagtgctt tagcacgagc ggtccagatt
    13681 taaagtagaa aatttcccgc ccactagggt taaaggtgtt cacactataa aagcatatac
    13741 gatgtgatgg tatttgatgg agcgtatatt gtatcaggta tttccgttgg atacgaatta
    13801 ttcgtacgac cctcatagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
    13861 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
    13921 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
    13981 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
    14041 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
    14101 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
    14161 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
    14221 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
    14281 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
    14341 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
    14401 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
    14461 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
    14521 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
    14581 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
    14641 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
    14701 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
    14761 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
    14821 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
    14881 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
    14941 tgttcgagcc gcccgcgcac ggctcaaccg tgcggctgca tgaaatcctg gccggtttgt
    15001 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
    15061 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
    15121 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
    15181 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
    15241 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
    15301 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
    15361 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
    15421 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
    15481 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
    15541 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
    15601 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
    15661 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
    15721 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
    15781 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
    15841 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
    15901 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
    15961 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
    16021 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
    16081 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
    16141 cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc
    16201 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
    16261 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
    16321 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
    16381 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
    16441 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
    16501 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
    16561 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
    16621 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
    16681 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
    16741 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
    16801 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
    16861 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
    16921 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
    16981 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
    17041 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
    17101 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
    17161 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
    17221 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
    17281 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
    17341 cgaaaaggcc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
    17401 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
    17461 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
    17521 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
    17581 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
    17641 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
    17701 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
    17761 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aaacggtcac
    17821 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
    17881 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
    17941 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    18001 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
    18061 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    18121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    18181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    18241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    18301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    18361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    18421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    18481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    18541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    18601 aggtat
//
