LOCUS       sequence-182432-        3215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP in the presence of Pseudomonas fluorescence sigma 
            factor ECF20_992.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3215)
  TITLE     Unpublished Murray Lab Plasmids
REFERENCE   2  (bases 1 to 3215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P20_992-deGFP
FEATURES             Location/Qualifiers
     source          1..3215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             41..63
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(100..121)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(361..380)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     708..729
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(934..951)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1105..1693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1185..1204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1864..2655,2656..2724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHATRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2487..2506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2725..2829)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2897..2915
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gttgaaccca tcagctgcgt tcatcagcga aagcaataat tttgtttaac tttaagaagg
       61 agatatacaa tggagctttt cactggcgtt gttcccatcc tggtcgagct ggacggcgac
      121 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      181 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      241 actaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      301 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      361 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      421 cgcatcgatc tgaatggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      481 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
      541 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
      601 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
      661 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
      721 gagttcgtga ccgccgccgg gatctaatga actaacgcat gaaaagcccc cggaagatca
      781 ccttccgggg gcttttttat tgcgcccttg agagtcgggc attgtcttcg ctccttccgg
      841 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tgttctttat catgcaactc
      901 gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      961 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1081 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     1801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     1861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     1921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     1981 ccccagtgct gcaatgatac cgcgtgaccc acgctcaccg gctccagatt tatcagcaat
     2041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2221 attcagctcc ggttcccaac gatcaaggcg agttgcatga tcccccatgt tgtgcaaaaa
     2281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2461 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt
     2521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     2701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     2761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     2821 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     2881 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtgttcaag aattctggcg
     2941 aatcctctga ccagccagaa aacgaccttt ctgtggtgaa accggatgct gcaattcaga
     3001 gcgccagcaa gtgggggaca gcagatgacc tgaccgccgc agagtggatg tttgacatgg
     3061 tgatgactat cgcaccatca gccagaaaac cgaattttgc tgggtgggct aacgatatcc
     3121 gcctgatgcg tgaacgtgac ggacgtaacg aagacaacca cgcatgcgcg gataaaaatt
     3181 tcatttgccc gcgacggatt ccccgcccat ctatc
//
