LOCUS       sequence-18244-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Shin DH, Brandsen J, Jancarik J, Yokota H, Kim R, Kim SH
  TITLE     Structural analyses of peptide release factor 1 from Thermotoga 
            maritima reveal domain flexibility required for its interaction with
            the ribosome.
  JOURNAL   J Mol Biol. 2004 Jul 30. 341(1):227-39.
  PUBMED    15312775
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2004 Jul 30. 341(1):227-39."
COMMENT     Sequence Label: pET21a.TM1363
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgaaa gaaaagaaaa aagagatcga
       61 aaagctcctc gccaggccag atctgactcc cgaacagatg aagaactacg ggatggaata
      121 cgcaaagata gaagaaatag agaacatcac aaacagaata aaagaaactc aagagttcat
      181 cgaacttttg agagaagaag gagaaaatga actggagata gaaaagtacg agaaagaact
      241 tgaccagctc tatcaagaac tgctctttct tctttctcca gaagcgagcg ataaagcaat
      301 cgtagaaatc agaccaggca cgggtggaga agaagccgct ctcttcgcgc gggatctgtt
      361 cagaatgtac accaggtacg cagaaagaaa aggctggaac ctcgaagtcg ctgaaatcca
      421 cgaaacggat ctcggtggaa taagggaagt ggtcttcttt gtgaagggaa agaacgctta
      481 cggtattttg aagtacgaaa gcggtgtcca tagagtccag agagttcctg ttacagagtc
      541 tggagggaga atccatacgt ccaccgctac cgttgccgtt cttccagaga ttgaagaaaa
      601 ggatatagaa atcaggccgg aagacttgaa gatagaaact ttcagggcat cgggacacgg
      661 tggacagtac gtcaacaaga ccgaatccgc tgtgaggatc acacatcttc caacgggaat
      721 agtggtctcg tgtcagaacg aaagatctca gtatcagaac aaacaaaccg ctctgaggat
      781 cctcagagca agactctacc agctacag
//
