LOCUS       sequence-182446-       20206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rosa26 gene targeting vector to induce a Cre-dependent mosaic of 
            cells expressing different fluorescent and marker proteins localized
            to the membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20206)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 20206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iMb-Control-Mosaic (IR98.10)
FEATURES             Location/Qualifiers
     source          1..20206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2218..2234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        2263..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2891..2909
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3356..3372
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3382..3400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3418..4500
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(4507..4572,4573..4610)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        4624..5003
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5005..5278
                     /label=chicken beta-actin promoter
     intron          5279..6295
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     6219..6241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     6303..6322
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(6356..6389)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(6397..6430)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6440..6473)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6487..6496
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6550..7049
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6585..6608)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6982..7001
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        7061..7108
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7126..7929
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7189..7208)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7799..7818
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7968..8192
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    8349..8483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8386..8405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8440..8459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(8503..8536)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      8659..8668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8665..8667,8668..8670,8671..9381)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8710..8731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8971..8990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9318..9339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9391..9444
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9475..9852
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             9877..9930
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    9990..10214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(10249..10282)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             10326..11753
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             11763..11816
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             11847..12224
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             12249..12302
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    12350..12574
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(12609..12642)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      12765..12774
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12771..13466
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             13485..13538
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             13569..13946
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             13971..14024
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     polyA_signal    14084..14308
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    14360..14407
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(14590..14607)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(14779..14795)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    14814..15038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    15126..19464
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(19514..19530)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(19563..19581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(19591..19607)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      19749..20204
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     20046..20067
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcacgtgt tcgaaacgcg ttgacattga ttattgacta
     2281 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact gcttactggc
     2881 ttatcgaaat taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa
     2941 gcttggtacc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     3001 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     3061 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     3121 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     3181 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     3241 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     3301 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     3361 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattgga gctccccgcg
     3421 gcaggccctc cgagcgtggt ggagccgttc tgtgagacag ccgggtacga gtcgtgacgc
     3481 tggaaggggc aagcgggtgg tgggcaggaa tgcggtccgc cctgcagcaa ccggaggggg
     3541 agggagaagg gagcggaaaa gtctccaccg gacgcggcca tggctcgggg gggggggggc
     3601 agcggaggag cgcttccggc cgacgtctcg tcgctgattg gcttcttttc ctcccgccgt
     3661 gtgtgaaaac acaaatggcg tgttttggtt ggcgtaaggc gcctgtcagt taacggcagc
     3721 cggagtgcgc agccgccggc agcctcgctc tgcccactgg gtggggcggg aggtaggtgg
     3781 ggtgaggcga gctggacgtg cgggcgcggt cggcctctgg cggggcgggg gaggggaggg
     3841 agggtcagcg aaagtagctc gcgcgcgagc ggccgcccac cctccccttc ctctggggga
     3901 gtcgttttac ccgccgccgg ccgggcctcg tcgtctgatt ggctctcggg gcccagaaaa
     3961 ctggcccttg ccattggctc gtgttcgtgc aagttgagtc catccgccgg ccagcggggg
     4021 cggcgaggag gcgctcccag gttccggccc tcccctcggc cccgcgccgc agagtctggc
     4081 cgcgcgcccc tgcgcaacgt ggcaggaagc gcgcgctggg ggcggggacg ggcagtaggg
     4141 ctgagcggct gcggggcggg tgcaagcacg tttccgactt gagttgcctc aagaggggcg
     4201 tgctgagcca gacctccatc gcgcactccg gggagtggag ggaaggagcg agggctcagt
     4261 tgggctgttt tggaggcagg aagcacttgc tctcccaaag tcgctctgag ttgttatcag
     4321 taagggagct gcagtggagt aggcggggag aaggccgcac ccttctccgg aggggggagg
     4381 ggagtgttgc aatacctttc tgggagttct ctgctgcctc ctggcttctg aggaccgccc
     4441 tgggcctggg agaatccctt ccccctcttc cctcgtgatc tgcaactcca gtctttctag
     4501 catctgtagg gcgcagtagt ccagggtttc cttgatgatg tcatacttat cctgtccctt
     4561 ttttttccac agctcgcggt tgaggacaaa ctcttcgcgg tctttccagt ggttaattaa
     4621 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     4681 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4741 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4801 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4861 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4921 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4981 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     5041 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     5101 cgatgggggc gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
     5161 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     5221 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     5281 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     5341 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     5401 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     5461 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     5521 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     5581 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     5641 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     5701 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     5761 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     5821 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     5881 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     5941 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     6001 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     6061 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     6121 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     6181 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     6241 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     6301 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattcc tcgagataac
     6361 ttcgtatagt ataccttata cgaagttatc gagctcataa cttcgtatag gatactttat
     6421 acgaagttat aacgtcgaga taacttcgta tagcatacat tatacgaagt tataccggca
     6481 gatctcgcca ccatggtgag tgtgattaaa ccagaggcta gcttggctgc aggtcgtcga
     6541 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     6601 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     6661 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     6721 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     6781 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     6841 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     6901 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     6961 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     7021 tctcctcttc ctcatctccg ggcctttcga cctgcagcct gttgacaatt aatcatcggc
     7081 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaacatggg atcggccatt
     7141 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     7201 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7261 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7321 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7381 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7441 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7501 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     7561 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     7621 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgat
     7681 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     7741 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     7801 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     7861 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     7921 ttcttctgag gggatcaatt ctctagagct cgctgatcag gcctcgactg tgccttctag
     7981 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     8041 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     8101 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     8161 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     8221 ctcgactaga gcttgcggaa cccttcgtaa tcagccatac cacatttgta gaggttttac
     8281 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     8341 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8461 atgtatctta tcatgtctgg atctgacatg gtaagtaagc ttataacttc gtatagtata
     8521 ccttatacga agttatggcg cgcctaattc acagccacca tgctgtgctg catcagaaga
     8581 actaaaccgg ttgagaagaa tgaagaggcc gatcaggagc tgcagtcgac ggtaccgcgg
     8641 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     8701 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     8761 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     8821 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
     8881 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     8941 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     9001 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     9061 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     9121 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     9181 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     9241 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc
     9301 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     9361 ggcatggacg agctgtacaa gggcagtgga gagggcagag gaagtctgct aacatgcggt
     9421 gacgtcgagg agaatcctgg cccagaattc tgtgtggata cctaccgcta catcatgcca
     9481 gagccagcga agtctgctcc cgccccgaaa aagggctcca agaaggcggt gactaaggcg
     9541 cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg agagctattc catctatgtg
     9601 tacaaggttc tgaagcaggt ccaccctgac accggcattt cgtccaaggc catgggcatc
     9661 atgaactcgt ttgtgaacga cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg
     9721 cattacaaca agcgctcgac catcacctcc agggagatcc agacggccgt gcgcctgctg
     9781 ctgcctgggg agttggccaa gcacgccgtg tccgagggca ctaaggccat caccaagtac
     9841 accagcgcta aggatccacc ggtcgcctga agtggagagg gcagaggaag tctgctaaca
     9901 tgcggtgacg tcgaggagaa tcctggccca gaattctgca gatatccagc acagtggcgg
     9961 ccgctcgagt ctaggcgcgc cagcctcgac tgtgccttct agttgccagc catctgttgt
    10021 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
    10081 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
    10141 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
    10201 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggagatctat aacttcgtat
    10261 aggatacttt atacgaagtt atggtaccgg cgcgccgatc catgggttgc tgtttctcca
    10321 agaccatggt gagcaaggga gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca
    10381 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
    10441 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg cccttcgcct
    10501 gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag caccccgccg
    10561 acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
    10621 acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag gacggcacgc
    10681 tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc gtaatgcaga
    10741 agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac ggcgtgctga
    10801 agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg gtggagttca
    10861 agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac gtggacacca
    10921 agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gagcgctccg
    10981 agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc ggcagctccg
    11041 gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg cgcttcaagg
    11101 tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag ggcgagggcc
    11161 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
    11221 tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac gtgaagcacc
    11281 ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
    11341 tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg
    11401 gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac ggccccgtaa
    11461 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg
    11521 tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccgc tacctggtgg
    11581 agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac tactacgtgg
    11641 acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
    11701 gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac aagggcagtg
    11761 gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagaat
    11821 tctgtgtgga tacctaccgc tacatcatgc cagagccagc gaagtctgct cccgccccga
    11881 aaaagggctc caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc
    11941 gcagccgcaa ggagagctat tccatctatg tgtacaaggt tctgaagcag gtccaccctg
    12001 acaccggcat ttcgtccaag gccatgggca tcatgaactc gtttgtgaac gacattttcg
    12061 agcgcatcgc aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct
    12121 ccagggagat ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg
    12181 tgtccgaggg cactaaggcc atcaccaagt acaccagcgc taaggatcca ccggtcgccg
    12241 gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc
    12301 cagaattctg cagatatcca gcacagtggc ggccgctcga gagcctcgac tgtgccttct
    12361 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    12421 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    12481 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    12541 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    12601 ggactagtat aacttcgtat agcatacatt atacgaagtt atggcgcgcc taattcacag
    12661 ccaccatgct gtgctgcatc agaagaacta agccggttga gaagaatgaa gaggccgatc
    12721 aggagctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagcg
    12781 agctgattaa ggagaacatg cacatgaagc tctacatgga gggcaccgtg aacaaccacc
    12841 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
    12901 tcaaggcggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
    12961 tgtacggcag caaaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
    13021 ccttccccga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
    13081 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
    13141 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
    13201 ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagagccgac atggccctga
    13261 agctcgtggg cgggggccac ctgatctgca acttgaagac cacatacaga tccaagaaac
    13321 ccgctaagaa cctcaagatg cccggcgtct actatgtgga cagaagactg gaaagaatca
    13381 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
    13441 acctccctag caaactgggg cacagaatgt acaagggcag tggagagggc agaggaagtc
    13501 tgctaacatg cggtgacgtc gaggagaatc ctggcccaga attctgtgtg gatacctacc
    13561 gctacatcat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc tccaagaagg
    13621 cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc aaggagagct
    13681 attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc atttcgtcca
    13741 aggccatggg catcatgaac tcgtttgtga acgacatttt cgagcgcatc gcaggtgagg
    13801 cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag atccagacgg
    13861 ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag ggcactaagg
    13921 ccatcaccaa gtacaccagc gctaaggatc caccggtcgc cggcagtgga gagggcagag
    13981 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccagaattc tgcagatatc
    14041 cagcacagtg gcggccgctc gagtctaggc gcgccagcct cgactgtgcc ttctagttgc
    14101 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
    14161 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
    14221 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
    14281 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggggcgg
    14341 ccgcgatcaa ttcggtaccg aagttcctat tccgaagttc ctattctcta gaaagtatag
    14401 gaacttcctc gagggtaccc caattccgcc cccccccccc ccctaacgtt actggccgaa
    14461 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
    14521 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
    14581 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
    14641 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
    14701 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacc gagctcggat ccactagtcc
    14761 agtgtggtgg aattggggga tccactagtt ctagagcggc cgatcagcct cgactgtgcc
    14821 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
    14881 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
    14941 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
    15001 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
    15061 ctggggctcg agatccacta gttctagcct cgaggctaga gcggccgccg cggatatcga
    15121 attcgctaga agatgggcgg gagtcttctg ggcaggctta aaggctaacc tggtgtgtgg
    15181 gcgttgtcct gcaggggaat tgaacaggtg taaaattgga gggacaagac ttcccacaga
    15241 ttttcggttt tgtcgggaag ttttttaata ggggcaaata aggaaaatgg gaggataggt
    15301 agtcatctgg ggttttatgc agcaaaacta caggttatta ttgcttgtga tccgcctcgg
    15361 agtattttcc atcgaggtag attaaagaca tgctcacccg agttttatac tctcctgctt
    15421 gagatcctta ctacagtatg aaattacagt gtcgcgagtt agactatgta agcagaattt
    15481 taatcatttt taaagagccc agtacttcat atccatttct cccgctcctt ctgcagcctt
    15541 atcaaaaggt attttagaac actcatttta gccccatttt catttattat actggcttat
    15601 ccaaccccta gacagagcat tggcattttc cctttcctga tcttagaagt ctgatgactc
    15661 atgaaaccag acagattagt tacatacacc acaaatcgag gctgtagctg gggcctcaac
    15721 actgcagttc ttttataact ccttagtaca ctttttgttg atcctttgcc ttgatcctta
    15781 attttcagtg tctatcacct ctcccgtcag gtggtgttcc acatttgggc ctattctcag
    15841 tccagggagt tttacaacaa tagatgtatt gagaatccaa cctaaagctt aactttccac
    15901 tcccatgaat gcctctctcc tttttctcca tttataaact gagctattaa ccattaatgg
    15961 tttccaggtg gatgtctcct cccccaatat tacctgatgt atcttacata ttgccaggct
    16021 gatattttaa gacattaaaa ggtatatttc attattgagc cacatggtat tgattactgc
    16081 ttactaaaat tttgtcattg tacacatctg taaaaggtgg ttccttttgg aatgcaaagt
    16141 tcaggtgttt gttgtctttc ctgacctaag gtcttgtgag cttgtatttt ttctatttaa
    16201 gcagtgcttt ctcttggact ggcttgactc atggcattct acacgttatt gctggtctaa
    16261 atgtgatttt gccaagcttc ttcaggacct ataattttgc ttgacttgta gccaaacaca
    16321 agtaaaatga ttaagcaaca aatgtatttg tgaagcttgg tttttaggtt gttgtgttgt
    16381 gtgtgcttgt gctctataat aatactatcc aggggctgga gaggtggctc ggagttcaag
    16441 agcacagact gctcttccag aagtcctgag ttcaattccc agcaaccaca tggtggctca
    16501 caaccatctg taatgggatc tgatgccctc ttctggtgtg tctgaagacc acaagtgtat
    16561 tcacattaaa taaataaatc ctccttcttc ttcttttttt tttttttaaa gagaatactg
    16621 tctccagtag aatttactga agtaatgaaa tactttgtgt ttgttccaat atggtagcca
    16681 ataatcaaat tactctttaa gcactggaaa tgttaccaag gaactaattt ttatttgaag
    16741 tgtaactgtg gacagaggag ccataactgc agacttgtgg gatacagaag accaatgcag
    16801 actttaatgt cttttctctt acactaagca ataaagaaat aaaaattgaa cttctagtat
    16861 cctatttgtt taaactgcta gctttactta acttttgtgc ttcatctata caaagctgaa
    16921 agctaagtct gcagccatta ctaaacatga aagcaagtaa tgataatttt ggatttcaaa
    16981 aatgtagggc cagagtttag ccagccagtg gtggtgcttg cctttatgcc tttaatccca
    17041 gcactctgga ggcagagaca ggcagatctc tgagtttgag cccagcctgg tctacacatc
    17101 aagttctatc taggatagcc aggaatacac acagaaaccc tgttggggag gggggctctg
    17161 agatttcata aaattataat tgaagcattc cctaatgagc cactatggat gtggctaaat
    17221 ccgtctacct ttctgatgag atttgggtat tattttttct gtctctgctg ttggttgggt
    17281 cttttgacac tgtgggcttt ctttaaagcc tccttcctgc catgtggtct cttgtttgct
    17341 actaacttcc catggcttaa atggcatggc tttttgcctt ctaagggcag ctgctgagat
    17401 ttgcagcctg atttccaggg tggggttggg aaatctttca aacactaaaa ttgtccttta
    17461 attttttttt taaaaaatgg gttatataat aaacctcata aaatagttat gaggagtgag
    17521 gtggactaat attaaatgag tccctcccct ataaaagagc tattaaggct ttttgtctta
    17581 tacttaactt tttttttaaa tgtggtatct ttagaaccaa gggtcttaga gttttagtat
    17641 acagaaactg ttgcatcgct taatcagatt ttctagttca aatccagaga atccaaattc
    17701 ttcacagcca aagtcaaatt aagaatttct gacttttaat gttaatttgc ttactgtgaa
    17761 tataaaaatg atagcttttc ctgaggcagg gtctcactat gtatctctgc ctgatctgca
    17821 acaagatatg tagactaaag ttctgcctgc ttttgtctcc tgaatactaa ggttaaaatg
    17881 tagtaatact tttggaactt gcaggtcaga ttcttttata ggggacacac taagggagct
    17941 tgggtgatag ttggtaaaat gtgtttcaag tgatgaaaac ttgaattatt atcaccgcaa
    18001 cctacttttt aaaaaaaaaa gccaggcctg ttagagcatg cttaagggat ccctaggact
    18061 tgctgagcac acaagagtag ttacttggca ggctcctggt gagagcatat ttcaaaaaac
    18121 aaggcagaca accaagaaac tacagttaag gttacctgtc tttaaaccat ctgcatatac
    18181 acagggatat taaaatattc caaataatat ttcattcaag ttttccccca tcaaattggg
    18241 acatggattt ctccggtgaa taggcagagt tggaaactaa acaaatgttg gttttgtgat
    18301 ttgtgaaatt gttttcaagt gatagttaaa gcccatgaga tacagaacaa agctgctatt
    18361 tcgaggtctc ttggtttata ctcagaagca cttctttggg tttccctgca ctatcctgat
    18421 catgtgctag gcctacctta ggctgattgt tgttcaaata aacttaagtt tcctgtcagg
    18481 tgatgtcata tgatttcata tatcaaggca aaacatgtta tatatgttaa acatttgtac
    18541 ttaatgtgaa agttaggtct tttgggtttg atttttaatt ttcaaaacct gagctaaata
    18601 agtcattttt acatgtctta catttggtgg aattgtataa ttgtggtttg caggcaagac
    18661 tctctgacct agtaacccta cctatagagc actttgctgg gtcacaagtc taggagtcaa
    18721 gcatttcacc ttgaagttga gacgttttgt tagtgtatac tagtttatat gttggaggac
    18781 atgtttatcc agaagatatt caggactatt tttgactggg ctaaggaatt gattctgatt
    18841 agcactgtta gtgagcattg agtggccttt aggcttgaat tggagtcact tgtatatctc
    18901 aaataatgct ggcctttttt aaaaagccct tgttctttat caccctgttt tctacataat
    18961 ttttgttcaa agaaatactt gtttggatct ccttttgaca acaatagcat gttttcaagc
    19021 catatttttt ttcctttttt tttttttttt tggtttttcg agacagggtt tctctgtata
    19081 gccctggctg tcctggaact cactttgtag accaggctgg cctcgaactc agaaatccgc
    19141 ctgcctctgc ctcctgagtg ccgggattaa aggcgtgcac caccacgcct ggctaagttg
    19201 gatattttgt tatataacta taaccaatac taactccact gggtggattt ttaattcagt
    19261 cagtagtctt aagtggtctt tattggccct tcattaaaat ctactgttca ctctaacaga
    19321 ggctgttggt actagtggca cttaagcaac ttcctacgga tatactagca gattaagggt
    19381 cagggataga aactagtcta gcgttttgta tacctaccag ctttatacta ccttgttctg
    19441 atagaaatat ttcaggacat ctagcttatc gatccgtcga cgttcgaaca cgtggaattc
    19501 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccaattc
    19561 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
    19621 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    19681 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    19741 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    19801 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    19861 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    19921 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    19981 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    20041 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    20101 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    20161 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttag
//
