LOCUS       sequence-18245-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Du X, Choi IG, Kim R, Wang W, Jancarik J, Yokota H, Kim SH
  TITLE     Crystal structure of an intracellular protease from Pyrococcus 
            horikoshii at 2-A resolution.
  JOURNAL   Proc Natl Acad Sci U S A. 2000 Dec 19. 97(26):14079-84.
  PUBMED    11114201
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2000 Dec 19. 97(26):14079-84."
COMMENT     Sequence Label: pHMMa.PH1704
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(632..650)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      646..693
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tcnctnnnnn ntttgtttaa ctttaagaag gagatataca tatgaaggta ctattcttaa
       61 cagcgaacga gtttgaggat gtggaactaa tttaccctta ccataggctt aaagaagagg
      121 gacatgaagt ttacatagca agctttgaaa ggggaacaat tacagggaaa catggatatt
      181 cagttaaggt tgacttgact ttcgataaag ttaatcccga ggagtttgat gcattagtcc
      241 ttccaggagg aagggcaccg gaaagggtaa gattaaatga gaaagctgtg agcatagcca
      301 gaaagatgtt cagtgaagga aagcccgttg ctagcatatg ccatggtcct caaatcttga
      361 tttctgctgg agttctaagg ggtagaaagg gaacaagcta tcctgggatt aaggatgaca
      421 tgataaatgc aggggttgaa tgggttgatg cagaggtagt tgttgatggg aactgggtta
      481 gctccagggt tccagctgat ctttatgcct ggatgaggga atttgtaaag ttacttaaat
      541 aaggatcccc gggtaccgag ctcgaattca tcgatgatat cagatccggc tgctaacaaa
      601 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
      661 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat
      721 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      781 gagcaggact gggcggcggc caaagcggtc ggacagngct ccgagaacgg gngcgcatag
      841 aaattgcatc aacg
//
