LOCUS       sequence-182454-       27430 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Second generation Rosa26 gene targeting vector to induce a 
            Cre-dependent mosaic of cells expressing different chromatin 
            localized fluorescent proteins and Notch signalling genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 27430)
  AUTHORS   Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, 
            Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, 
            Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera 
            S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R
  TITLE     Dual ifgMosaic: A Versatile Method for Multispectral and 
            Combinatorial Mosaic Gene-Function Analysis.
  JOURNAL   Cell. 2017 Aug 10;170(4):800-814.e18. doi: 
            10.1016/j.cell.2017.07.031.
  PUBMED    28802047
REFERENCE   2  (bases 1 to 27430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07"; journalName: "Cell"; date: "2017-08-10- 
            10"; volume: "170"; issue: "4"; pages: "800-814.e18"
COMMENT     Sequence Label: iChr2-Notch Mosaic (SO250)
FEATURES             Location/Qualifiers
     source          1..27430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2218..2234
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2219..2235
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(5845..5910,5911..5948)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     enhancer        5977..6356
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6358..6631
                     /label=chicken beta-actin promoter
     intron          6632..7648
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7572..7594
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7656..7675
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(7709..7742)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(7750..7783)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(7792..7825)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7839..7848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7884..7904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7905..8609
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    8792..8913
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8925..8958)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      8965..8974
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8977..8994
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8995..9369
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     CDS             9391..10101
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9536..9554)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9804..9823
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    10115..10703
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10586..10597)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10837..10958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10973..11006)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      11014..11023
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11020..11400
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MAPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYK
                     VLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLL
                     PGELAKHAVSEGTKAITKYTSAK"
     regulatory      11419..11428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(11425..11427,11428..11430,11431..12138)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(11425..11427,11428..11430,11431..12138)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             12142..12183
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             12193..12246
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    12491..13079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(12962..12973)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    13213..13334
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(13348..13381)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      13388..13397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13400..13426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             13427..13801
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     regulatory      13820..13829
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(13826..13828,13829..13831,13832..14542)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             14552..14605
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             16970..16993
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    17010..17598
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(17481..17492)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    17732..17853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    17891..17924
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        17988..18487
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(18023..18046)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     18420..18439
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        18499..18546
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             18565..19368
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(18628..18647)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     19238..19257
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(19400..19417)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    19406..19630
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    19688..19721
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    22350..26688
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     primer_bind     complement(26738..26754)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(26738..26754)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(26786..26805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(26787..26805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(26815..26832)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(26815..26831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(26824..26846)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      26973..27428
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(27060..27079)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     27270..27291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgacgg tatcgataag cttcacgtgt tcgaaacgcg gtccgccctg cagcaaccgg
     2281 agggggaggg agaagggagc ggaaaagtct ccaccggacg cggccatggc tcgggggggg
     2341 gggggcagcg gaggagcgct tccggccgac gtctcgtcgc tgattggctt cttttcctcc
     2401 cgccgtgtgt gaaaacacaa atggcgtgtt ttggttggcg taaggcgcct gtcagttaac
     2461 ggcagccgga gtgcgcagcc gccggcagcc tcgctctgcc cactgggtgg ggcgggaggt
     2521 aggtggggtg aggcgagctg gacgtgcggg cgcggtcggc ctctggcggg gcgggggagg
     2581 ggagggaggg tcagcgaaag tagctcgcgc gcgagcggcc gcccaccctc cccttcctct
     2641 gggggagtcg ttttacccgc cgccggccgg gcctcgtcgt ctgattggct ctcggggccc
     2701 agaaaactgg cccttgccat tggctcgtgt tcgtgcaagt tgagtccatc cgccggccag
     2761 cgggggcggc gaggaggcgc tcccaggttc cggccctccc ctcggccccg cgccgcagag
     2821 tctggccgcg cgcccctgcg caacgtggca ggaagcgcgc gctgggggcg gggacgggca
     2881 gtagggctga gcggctgcgg ggcgggtgca agcacgtttc cgacttgagt tgcctcaaga
     2941 ggggcgtgct gagccagacc tccatcgcgc actccgggga gtggagggaa ggagcgaggg
     3001 ctcagttggg ctgttttgga ggcaggaagc acttgctctc ccaaagtcgc tctgagttgt
     3061 tatcagtaag ggagctgcag tggagtaggc ggggagaagg ccgcaccctt ctccggaggg
     3121 gggaggggag tgttgcaata cctttctggg agttctctgc tgcctcctgg cttctgagga
     3181 ccgccctggg cacatgatgg acctgtcgac ccccatcctc actgactccg tcctggagta
     3241 ggatgagaga taatggcctt acgttgtgcc aggggagggt cgggctggat ttagcaagat
     3301 ttaccttctc caaagagcgg tgctgcagtg gcacagctgc ccacggaggt gggggggtca
     3361 ccgtccctgg aggtggtgaa gaactgtggg gatgtggcac tgagggacat ggccagtggg
     3421 cacggtgggt gggttggggt tggtcttggg gatcttggag ggcttttcca gccttcatga
     3481 tttgacgatt gtatgaacat ctacatggca attctccagc tgcctgtccc agtcctactg
     3541 acccagctgt atctctccag gcaagctctt ccaccccttc tgcttgcatc cagacaccat
     3601 caaacatgca ggctcagaca cagggaccag cagtgtctgt ggcctttttg tgctcctctc
     3661 catgctgggt tttaacttgt tctttgtcct tctatcctat cttctgatcc ttaaggctgt
     3721 tctgaacgct gtgacttgga gagtgtccca gagccctcaa cacctgcatg tcccacgtcc
     3781 atgctgtcct gctcttcctt atccccaaga tctgcctctc cgtgatgcac tgaattggca
     3841 aacatgtgtc accccagacc aacaatgtca cagcaaactc ccccttgata ggacaggagg
     3901 gaatggcttt aaactgagac aggggaggtt taggttggat atgaggaggc agtttttccc
     3961 ccagagggtg gtgacgcact gaacaggttg cccaaggagg ctgtggatgc cccatccctg
     4021 caggcattca aggccaggct ggatgtggct ctgggcagcc tgggctgctg gttggcgacc
     4081 ctgcacatag cagggggttg gaactggatg agcattgtgg gcctttccaa cccaggccat
     4141 tctatgattc tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc
     4201 caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca
     4261 ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgtc
     4321 gggccggagc ggagccccgg gcggctcgct gctgccccct agcgggagag ggacgcaatt
     4381 acagccctgg gggctttggg ggggggctct ccccgtgagc tcccgcggac gcccccttcc
     4441 aggaccttcc aggaaagcct gcttcttttc ttcaaccttt ccccccatcc tcactgactc
     4501 cgtcctggag taggatgaga gataatggcc ttacgttgtg ccaggggagg gtcgggctgg
     4561 atttagcaag atttaccttc tccaaagagc ggtgctgcag tggcacagct gcccacggag
     4621 gtgggggggt caccgtccct ggaggtggtg aagaactgtg gggatgtggc actgagggac
     4681 atggccagtg ggcacggtgg gtgggttggg gttggtcttg gggatcttgg agggcttttc
     4741 cagccttcat gatttgacga ttgtatgaac atctacatgg caattctcca gctgcctgtc
     4801 ccagtcctac tgacccagct gtatctctcc aggcaagctc ttccacccct tctgcttgca
     4861 tccagacacc atcaaacatg caggctcaga cacagggacc agcagtgtct gtggcctttt
     4921 tgtgctcctc tccatgctgg gttttaactt gttctttgtc cttctatcct atcttctgat
     4981 ccttaaggct gttctgaacg ctgtgacttg gagagtgtcc cagagccctc aacacctgca
     5041 tgtcccacgt ccatgctgtc ctgctcttcc ttatccccaa gatctgcctc tccgtgatgc
     5101 actgaattgg caaacatgtg tcaccccaga ccaacaatgt cacagcaaac tcccccttga
     5161 taggacagga gggaatggct ttaaactgag acaggggagg tttaggttgg atatgaggag
     5221 gcagtttttc ccccagaggg tggtgacgca ctgaacaggt tgcccaagga ggctgtggat
     5281 gccccatccc tgcaggcatt caaggccagg ctggatgtgg ctctgggcag cctgggctgc
     5341 tggttggcga ccctgcacat agcagggggt tggaactgga tgagcattgt gggcctttcc
     5401 aacccaggcc attctatgat tctgtcattc taaatctctc tttcagccta aagctttttc
     5461 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     5521 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     5581 ggggggagcg tcgggccgga gcggagcccc gggcggctcg ctgctgcccc ctagcgggag
     5641 agggacgcaa ttacagccct gggggctttg ggggggggct ctccccgtga gctcccgcgg
     5701 acgccccctt ccaggacctt ccaggaaagc ctgcttcttt tcttcaacct ttcttaattg
     5761 gccgtggcta gtcgaaactg ggcctgggag aatcccttcc ccctcttccc tcgtgatctg
     5821 caactccagt ctttctagca tctgtagggc gcagtagtcc agggtttcct tgatgatgtc
     5881 atacttatcc tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc
     5941 tttccagtgg ttaatgcacc ttccttcctt cgagtcgaca ttgattattg actagttatt
     6001 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     6061 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6121 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6181 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6241 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6301 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
     6361 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
     6421 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggcgcgc
     6481 gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag gtgcggcggc
     6541 agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg
     6601 gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc ttcgccccgt
     6661 gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc
     6721 acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg
     6781 acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt
     6841 gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
     6901 cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct
     6961 ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg
     7021 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     7081 cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc
     7141 ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg
     7201 gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg
     7261 ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     7321 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     7381 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     7441 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     7501 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     7561 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     7621 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     7681 tcatcatttt ggcaaagaat tcctcgagat aacttcgtat agtatacctt atacgaagtt
     7741 atcgagctca taacttcgta taggatactt tatacgaagt tataactcga gataacttcg
     7801 tatagcatac attatacgaa gttataccgg cagatctcgc caccatggtg agtgtgatta
     7861 cagaggctag cttcaccatg gctcctaaga agaagaggaa ggtgatgagc agcggcgccc
     7921 tgctgttcca cggcaagatc ccctacgtgg tggagatgga gggcaatgtg gatggccaca
     7981 ccttcagcat ccgcggcaag ggctacggcg atgccagcgt gggcaaggtg gatgcccagt
     8041 tcatctgcac cacaggcgat gtgcccgtgc cctggagcac cctggtgacc accctgaccg
     8101 caggcgccca gtgcttcgcc aagtacggcc ccgagctgaa ggatttctac aagagctgca
     8161 tgcccgatgg ctacgtgcag gagcgcacca tcaccttcga gggtgatggc aatttcaaga
     8221 cccgcgccga ggtgaccttc gagaatggca gcgtgtacaa tcgcgtgaag ctgaatggcc
     8281 agggcttcaa gaaggatggc cacgtgctgg gcaagaatct ggagttcaat ttcacccccc
     8341 actgcctgta catctggggc gatcaggcca atcacggcct gaagagcgcc ttcaagatct
     8401 gccacgagat caccggcagc aagggcgatt tcatcgtggc cgatcacacc cagatgaata
     8461 cccccatcgg cggcggcccc gtgcacgtgc ccgagtacca ccacatgagc taccacgtga
     8521 agctgagcaa ggatgtgacc gatcaccgcg ataatatgag cctgaaggag accgtgcgcg
     8581 ccgtggattg ccgcaagacc tacctgtgac gcttaattag ctgagcttgg actcctgttg
     8641 atagatccag taatgacctc agaactccat ctggccgcga ctctagatca taatcagcca
     8701 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     8761 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     8821 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8881 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgaa gcttataact tcgtatagta
     8941 taccttatac gaagttatcg gcgcgccacc atggcgcatc atcatcatca tcatccagag
     9001 ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag
     9061 aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtctac
     9121 aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg
     9181 aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat
     9241 tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg
     9301 cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc
     9361 agcgctaagg atccccgggt accggtaacc atggtgagca agggcgagga ggataacatg
     9421 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     9481 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     9541 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     9601 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     9661 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     9721 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     9781 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     9841 tcctccgaga gaatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     9901 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     9961 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
    10021 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc cactggcggc
    10081 atggacgagc tgtacaagtg aggcgcgccc cgctaatcaa cctctggatt acaaaatttg
    10141 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
    10201 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
    10261 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
    10321 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
    10381 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
    10441 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
    10501 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
    10561 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
    10621 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
    10681 ctccctttgg gccgcctccc cgcatcgata ccgtcgagac tgctgcacca gatcataatc
    10741 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
    10801 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
    10861 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
    10921 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtggagat ctataacttc
    10981 gtataggata ctttatacga agttatcggt accgccacca tggcgccaga gccagcgaag
    11041 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
    11101 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtcta caaggttctg
    11161 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaactcgttt
    11221 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
    11281 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
    11341 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
    11401 gatccccggg taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
    11461 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
    11521 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
    11581 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
    11641 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
    11701 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
    11761 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
    11821 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
    11881 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
    11941 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
    12001 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
    12061 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
    12121 ggcatggacg agctgtacat tggtaagcct atccctaacc ctctcctcgg tctcgattct
    12181 acgggcagtg gagagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
    12241 ggcccagaat tcctacccac ctgccccatg gcggagttcg cgctgccgcg gcacagcgcg
    12301 gtcatggagc gcctccgccg ccgcatcgaa ctgtgccggc gccaccacag cacctgtgag
    12361 gcccgctacg aggctgtgtc tcccgagcgc ctggagctgg agcgccagca cacctttgcc
    12421 ctgcaccagc gctgcatcca ggccaaggcc aagcgcgccg gcaagcacta actcgagggc
    12481 gcgccccgct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
    12541 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
    12601 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
    12661 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
    12721 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
    12781 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
    12841 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
    12901 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
    12961 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
    13021 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
    13081 tcgataccgt cgagactgct gcaccagatc ataatcagcc ataccacatt tgtagaggtt
    13141 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    13201 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    13261 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    13321 atcaatgtat cttaaggcgt gactagtata acttcgtata gcatacatta tacgaagtta
    13381 tcggcgcgcc accatggcgt atccatatga cgtcccagac tatgccccag agccagcgaa
    13441 gtctgctccc gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg
    13501 cggcaagaag cgcaagcgca gccgcaagga gagctattcc atctatgtct acaaggttct
    13561 gaagcaggtc caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaactcgtt
    13621 tgtgaacgac attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa
    13681 gcgctcgacc atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga
    13741 gttggccaag cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa
    13801 ggatccccgg gtaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
    13861 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
    13921 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
    13981 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
    14041 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
    14101 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
    14161 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
    14221 ggaggacggc aacatcctgg ggcacaagct ggagtacaac gccatcagcg acaacgtcta
    14281 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
    14341 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
    14401 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
    14461 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
    14521 cggcatggac gagctgtaca agggcagtgg agagggcaga ggaagtctgc taacatgcgg
    14581 tgacgtcgag gagaatcctg gcccagaatt ccctgagggc ttcaaagtgt ctgaggccag
    14641 caagaagaag cggcgggagc ccctcggcga ggactccgtg ggcctcaagc ccctgaagaa
    14701 cgcttcagac ggtgccctca tggacgacaa ccagaatgag tggggggacg aggacctgga
    14761 gaccaagaag ttccggttcg aggagcccgt ggttctgcct gacctggacg accagacaga
    14821 ccaccggcag tggactcagc agcacctgga tgccgctgac ctgcgcatgt ctgccatggc
    14881 ccccacaccg ccccagggtg aggttgacgc cgactgcatg gacgtcaatg tccgcgggcc
    14941 tgatggcttc accccgctca tgatcgcctc ctgcagcggg ggcggcctgg agacgggcaa
    15001 cagcgaggaa gaggaggacg cgccggccgt catctccgac ttcatctacc agggcgccag
    15061 cctgcacaac cagacagacc gcacgggcga gaccgccttg cacctggccg cccgctactc
    15121 acgctctgat gccgccaagc gcctgctgga ggccagcgca gatgccaaca tccaggacaa
    15181 catgggccgc accccgctgc atgcggctgt gtctgccgac gcacaaggtg tcttccagat
    15241 cctgatccgg aaccgagcca cagacctgga tgcccgcatg catgatggca cgacgccact
    15301 gatcctggct gcccgcctgg ccgtggaggg catgctggag gacctcatca actcacacgc
    15361 cgacgtcaac gccgtagatg acctgggcaa gtccgccctg cactgggccg ccgccgtgaa
    15421 caatgtggat gccgcagttg tgctcctgaa gaacggggct aacaaagata tgcagaacaa
    15481 cagggaggag acacccctgt ttctggccgc ccgggagggc agctacgaga ccgccaaggt
    15541 gctgctggac cactttgcca accgggacat cacggatcat atggaccgcc tgccgcgcga
    15601 catcgcacag gagcgcatgc atcacgacat cgtgaggctg ctggacgagt acaacctggt
    15661 gcgcagcccg cagctgcacg gagccccgct ggggggcacg cccaccctgt cgcccccgct
    15721 ctgctcgccc aacggctacc tgggcagcct caagcccggc gtgcagggca agaaggtccg
    15781 caagcccagc agcaaaggcc tggcctgtgg aagcaaggag gccaaggacc tcaaggcacg
    15841 gaggaagaag tcccaggatg gcaagggctg cctgctggac agctccggca tgctctcgcc
    15901 cgtggactcc ctggagtcac cccatggcta cctgtcagac gtggcctcgc cgccactgct
    15961 gccctccccg ttccagcagt ctccgtccgt gcccctcaac cacctgcctg ggatgcccga
    16021 cacccacctg ggcatcgggc acctgaacgt ggcggccaag cccgagatgg cggcgctggg
    16081 tgggggcggc cggctggcct ttgagactgg cccacctcgt ctctcccacc tgcctgtggc
    16141 ctctggcacc agcaccgtcc tgggctccag cagcggaggg gccctgaatt tcactgtggg
    16201 cgggtccacc agtttgaatg gtcaatgcga gtggctgtcc cggctgcaga gcggcatggt
    16261 gccgaaccaa tacaaccctc tgcgggggag tgtggcacca ggccccctga gcacacaggc
    16321 cccctccctg cagcatggca tggtaggccc gctgcacagt agccttgctg ccagcgccct
    16381 gtcccagatg atgagctacc agggcctgcc cagcacccgg ctggccaccc agcctcacct
    16441 ggtgcagacc cagcaggtgc agccacaaaa cttacagatg cagcagcaga acctgcagcc
    16501 agcaaacatc cagcagcagc aaagcctgca gccgccacca ccaccaccac agccgcacct
    16561 tggcgtgagc tcagcagcca gcggccacct gggccggagc ttcctgagtg gagagccgag
    16621 ccaggcagac gtgcagccac tgggccccag cagcctggcg gtgcacacta ttctgcccca
    16681 ggagagcccc gccctgccca cgtcgctgcc atcctcgctg gtcccacccg tgaccgcagc
    16741 ccagttcctg acgcccccct cgcagcacag ctactcctcg cctgtggaca acacccccag
    16801 ccaccagcta caggtgcctg agcacccctt cctgacccct tcgccggagt cgcccgacca
    16861 atggtcgtcc tcgtcgccgc actctaatgt gtctgactgg tctgagggcg tgtcgtcgcc
    16921 cccgccctcc atgcagtccc agatcgcgcg catcccggag gccttcaagg actacaagga
    16981 tgacgatgac aaataaggcg cgccccgcta atcaacctct ggattacaaa atttgtgaaa
    17041 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
    17101 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
    17161 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
    17221 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
    17281 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
    17341 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
    17401 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    17461 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    17521 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
    17581 tttgggccgc ctccccgcat cgataccgtc gagactgctg caccagatca taatcagcca
    17641 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    17701 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    17761 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    17821 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgtg gcggccgcag catagggatc
    17881 cccgaattcc gaagttccta ttctctagaa agtataggaa cttcaggtct gaagaggagt
    17941 ttacgtccag ccaagctagc ttggctgcag gtcgtcgaaa ttctaccggg taggggaggc
    18001 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
    18061 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
    18121 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
    18181 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
    18241 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
    18301 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
    18361 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
    18421 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
    18481 cctttcgacc tgcagcctgt tgacaattaa tcatcggcat agtatatcgg catagtataa
    18541 tacgacaagg tgaggaacta aaccatggga tcggccattg aacaagatgg attgcacgca
    18601 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
    18661 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
    18721 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
    18781 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
    18841 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
    18901 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
    18961 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
    19021 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
    19081 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg atctcgtcgt gacccatggc
    19141 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
    19201 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
    19261 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
    19321 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagg ggatcaattc
    19381 tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
    19441 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
    19501 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
    19561 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
    19621 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgactagagc ttgcggaacc
    19681 cttcgttgaa gttcctattc tctagaaagt ataggaactt cagtcgacgt gctccaagga
    19741 gagtttgctg gcaagatctt cggggtcgat gctaacacag ggaatgtgct tgcctatgag
    19801 aggctagacc gggagaaagt ctctgagtat cgtcgacccc catcctcact gactccgtcc
    19861 tggagtagga tgagagataa tggccttacg ttgtgccagg ggagggtcgg gctggattta
    19921 gcaagattta ccttctccaa agagcggtgc tgcagtggca cagctgccca cggaggtggg
    19981 ggggtcaccg tccctggagg tggtgaagaa ctgtggggat gtggcactga gggacatggc
    20041 cagtgggcac ggtgggtggg ttggggttgg tcttggggat cttggagggc ttttccagcc
    20101 ttcatgattt gacgattgta tgaacatcta catggcaatt ctccagctgc ctgtcccagt
    20161 cctactgacc cagctgtatc tctccaggca agctcttcca ccccttctgc ttgcatccag
    20221 acaccatcaa acatgcaggc tcagacacag ggaccagcag tgtctgtggc ctttttgtgc
    20281 tcctctccat gctgggtttt aacttgttct ttgtccttct atcctatctt ctgatcctta
    20341 aggctgttct gaacgctgtg acttggagag tgtcccagag ccctcaacac ctgcatgtcc
    20401 cacgtccatg ctgtcctgct cttccttatc cccaagatct gcctctccgt gatgcactga
    20461 attggcaaac atgtgtcacc ccagaccaac aatgtcacag caaactcccc cttgatagga
    20521 caggagggaa tggctttaaa ctgagacagg ggaggtttag gttggatatg aggaggcagt
    20581 ttttccccca gagggtggtg acgcactgaa caggttgccc aaggaggctg tggatgcccc
    20641 atccctgcag gcattcaagg ccaggctgga tgtggctctg ggcagcctgg gctgctggtt
    20701 ggcgaccctg cacatagcag ggggttggaa ctggatgagc attgtgggcc tttccaaccc
    20761 aggccattct atgattctgt cattctaaat ctctctttca gcctaaagct ttttccccgt
    20821 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
    20881 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
    20941 gagcgtcggg ccggagcgga gccccgggcg gctcgctgct gccccctagc gggagaggga
    21001 cgcaattaca gccctggggg ctttgggggg gggctctccc cgtgagctcc cgcggacgcc
    21061 cccttccagg accttccagg aaagcctgct tcttttcttc aacctttccc cccatcctca
    21121 ctgactccgt cctggagtag gatgagagat aatggcctta cgttgtgcca ggggagggtc
    21181 gggctggatt tagcaagatt taccttctcc aaagagcggt gctgcagtgg cacagctgcc
    21241 cacggaggtg ggggggtcac cgtccctgga ggtggtgaag aactgtgggg atgtggcact
    21301 gagggacatg gccagtgggc acggtgggtg ggttggggtt ggtcttgggg atcttggagg
    21361 gcttttccag ccttcatgat ttgacgattg tatgaacatc tacatggcaa ttctccagct
    21421 gcctgtccca gtcctactga cccagctgta tctctccagg caagctcttc caccccttct
    21481 gcttgcatcc agacaccatc aaacatgcag gctcagacac agggaccagc agtgtctgtg
    21541 gcctttttgt gctcctctcc atgctgggtt ttaacttgtt ctttgtcctt ctatcctatc
    21601 ttctgatcct taaggctgtt ctgaacgctg tgacttggag agtgtcccag agccctcaac
    21661 acctgcatgt cccacgtcca tgctgtcctg ctcttcctta tccccaagat ctgcctctcc
    21721 gtgatgcact gaattggcaa acatgtgtca ccccagacca acaatgtcac agcaaactcc
    21781 cccttgatag gacaggaggg aatggcttta aactgagaca ggggaggttt aggttggata
    21841 tgaggaggca gtttttcccc cagagggtgg tgacgcactg aacaggttgc ccaaggaggc
    21901 tgtggatgcc ccatccctgc aggcattcaa ggccaggctg gatgtggctc tgggcagcct
    21961 gggctgctgg ttggcgaccc tgcacatagc agggggttgg aactggatga gcattgtggg
    22021 cctttccaac ccaggccatt ctatgattct gtcattctaa atctctcttt cagcctaaag
    22081 ctttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga agcgttcaga
    22141 ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc tgccggctcg
    22201 gggatgcggg gggagcgtcg ggccggagcg gagccccggg cggctcgctg ctgcccccta
    22261 gcgggagagg gacgcaatta cagccctggg ggctttgggg gggggctctc cccgtgagct
    22321 taattaacgg cggcggagta tcgaattcgc tagaagatgg gcgggagtct tctgggcagg
    22381 cttaaaggct aacctggtgt gtgggcgttg tcctgcaggg gaattgaaca ggtgtaaaat
    22441 tggagggaca agacttccca cagattttcg gttttgtcgg gaagtttttt aataggggca
    22501 aataaggaaa atgggaggat aggtagtcat ctggggtttt atgcagcaaa actacaggtt
    22561 attattgctt gtgatccgcc tcggagtatt ttccatcgag gtagattaaa gacatgctca
    22621 cccgagtttt atactctcct gcttgagatc cttactacag tatgaaatta cagtgtcgcg
    22681 agttagacta tgtaagcaga attttaatca tttttaaaga gcccagtact tcatatccat
    22741 ttctcccgct ccttctgcag ccttatcaaa aggtatttta gaacactcat tttagcccca
    22801 ttttcattta ttatactggc ttatccaacc cctagacaga gcattggcat tttccctttc
    22861 ctgatcttag aagtctgatg actcatgaaa ccagacagat tagttacata caccacaaat
    22921 cgaggctgta gctggggcct caacactgca gttcttttat aactccttag tacacttttt
    22981 gttgatcctt tgccttgatc cttaattttc agtgtctatc acctctcccg tcaggtggtg
    23041 ttccacattt gggcctattc tcagtccagg gagttttaca acaatagatg tattgagaat
    23101 ccaacctaaa gcttaacttt ccactcccat gaatgcctct ctcctttttc tccatttata
    23161 aactgagcta ttaaccatta atggtttcca ggtggatgtc tcctccccca atattacctg
    23221 atgtatctta catattgcca ggctgatatt ttaagacatt aaaaggtata tttcattatt
    23281 gagccacatg gtattgatta ctgcttacta aaattttgtc attgtacaca tctgtaaaag
    23341 gtggttcctt ttggaatgca aagttcaggt gtttgttgtc tttcctgacc taaggtcttg
    23401 tgagcttgta ttttttctat ttaagcagtg ctttctcttg gactggcttg actcatggca
    23461 ttctacacgt tattgctggt ctaaatgtga ttttgccaag cttcttcagg acctataatt
    23521 ttgcttgact tgtagccaaa cacaagtaaa atgattaagc aacaaatgta tttgtgaagc
    23581 ttggttttta ggttgttgtg ttgtgtgtgc ttgtgctcta taataatact atccaggggc
    23641 tggagaggtg gctcggagtt caagagcaca gactgctctt ccagaagtcc tgagttcaat
    23701 tcccagcaac cacatggtgg ctcacaacca tctgtaatgg gatctgatgc cctcttctgg
    23761 tgtgtctgaa gaccacaagt gtattcacat taaataaata aatcctcctt cttcttcttt
    23821 tttttttttt taaagagaat actgtctcca gtagaattta ctgaagtaat gaaatacttt
    23881 gtgtttgttc caatatggta gccaataatc aaattactct ttaagcactg gaaatgttac
    23941 caaggaacta atttttattt gaagtgtaac tgtggacaga ggagccataa ctgcagactt
    24001 gtgggataca gaagaccaat gcagacttta atgtcttttc tcttacacta agcaataaag
    24061 aaataaaaat tgaacttcta gtatcctatt tgtttaaact gctagcttta cttaactttt
    24121 gtgcttcatc tatacaaagc tgaaagctaa gtctgcagcc attactaaac atgaaagcaa
    24181 gtaatgataa ttttggattt caaaaatgta gggccagagt ttagccagcc agtggtggtg
    24241 cttgccttta tgcctttaat cccagcactc tggaggcaga gacaggcaga tctctgagtt
    24301 tgagcccagc ctggtctaca catcaagttc tatctaggat agccaggaat acacacagaa
    24361 accctgttgg ggaggggggc tctgagattt cataaaatta taattgaagc attccctaat
    24421 gagccactat ggatgtggct aaatccgtct acctttctga tgagatttgg gtattatttt
    24481 ttctgtctct gctgttggtt gggtcttttg acactgtggg ctttctttaa agcctccttc
    24541 ctgccatgtg gtctcttgtt tgctactaac ttcccatggc ttaaatggca tggctttttg
    24601 ccttctaagg gcagctgctg agatttgcag cctgatttcc agggtggggt tgggaaatct
    24661 ttcaaacact aaaattgtcc tttaattttt tttttaaaaa atgggttata taataaacct
    24721 cataaaatag ttatgaggag tgaggtggac taatattaaa tgagtccctc ccctataaaa
    24781 gagctattaa ggctttttgt cttatactta actttttttt taaatgtggt atctttagaa
    24841 ccaagggtct tagagtttta gtatacagaa actgttgcat cgcttaatca gattttctag
    24901 ttcaaatcca gagaatccaa attcttcaca gccaaagtca aattaagaat ttctgacttt
    24961 taatgttaat ttgcttactg tgaatataaa aatgatagct tttcctgagg cagggtctca
    25021 ctatgtatct ctgcctgatc tgcaacaaga tatgtagact aaagttctgc ctgcttttgt
    25081 ctcctgaata ctaaggttaa aatgtagtaa tacttttgga acttgcaggt cagattcttt
    25141 tataggggac acactaaggg agcttgggtg atagttggta aaatgtgttt caagtgatga
    25201 aaacttgaat tattatcacc gcaacctact ttttaaaaaa aaaagccagg cctgttagag
    25261 catgcttaag ggatccctag gacttgctga gcacacaaga gtagttactt ggcaggctcc
    25321 tggtgagagc atatttcaaa aaacaaggca gacaaccaag aaactacagt taaggttacc
    25381 tgtctttaaa ccatctgcat atacacaggg atattaaaat attccaaata atatttcatt
    25441 caagttttcc cccatcaaat tgggacatgg atttctccgg tgaataggca gagttggaaa
    25501 ctaaacaaat gttggttttg tgatttgtga aattgttttc aagtgatagt taaagcccat
    25561 gagatacaga acaaagctgc tatttcgagg tctcttggtt tatactcaga agcacttctt
    25621 tgggtttccc tgcactatcc tgatcatgtg ctaggcctac cttaggctga ttgttgttca
    25681 aataaactta agtttcctgt caggtgatgt catatgattt catatatcaa ggcaaaacat
    25741 gttatatatg ttaaacattt gtacttaatg tgaaagttag gtcttttggg tttgattttt
    25801 aattttcaaa acctgagcta aataagtcat ttttacatgt cttacatttg gtggaattgt
    25861 ataattgtgg tttgcaggca agactctctg acctagtaac cctacctata gagcactttg
    25921 ctgggtcaca agtctaggag tcaagcattt caccttgaag ttgagacgtt ttgttagtgt
    25981 atactagttt atatgttgga ggacatgttt atccagaaga tattcaggac tatttttgac
    26041 tgggctaagg aattgattct gattagcact gttagtgagc attgagtggc ctttaggctt
    26101 gaattggagt cacttgtata tctcaaataa tgctggcctt ttttaaaaag cccttgttct
    26161 ttatcaccct gttttctaca taatttttgt tcaaagaaat acttgtttgg atctcctttt
    26221 gacaacaata gcatgttttc aagccatatt ttttttcctt tttttttttt tttttggttt
    26281 ttcgagacag ggtttctctg tatagccctg gctgtcctgg aactcacttt gtagaccagg
    26341 ctggcctcga actcagaaat ccgcctgcct ctgcctcctg agtgccggga ttaaaggcgt
    26401 gcaccaccac gcctggctaa gttggatatt ttgttatata actataacca atactaactc
    26461 cactgggtgg atttttaatt cagtcagtag tcttaagtgg tctttattgg cccttcatta
    26521 aaatctactg ttcactctaa cagaggctgt tggtactagt ggcacttaag caacttccta
    26581 cggatatact agcagattaa gggtcaggga tagaaactag tctagcgttt tgtataccta
    26641 ccagctttat actaccttgt tctgatagaa atatttcagg acatctagct tatcgatccg
    26701 tcgacgttcg aacacgtgga attcctgcag cccgggggat ccactagttc tagagcggcc
    26761 gccaccgcgg tggagctcca attcgcccta tagtgagtcg tattacgcgc gctcactggc
    26821 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    26881 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    26941 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
    27001 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    27061 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    27121 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    27181 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    27241 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    27301 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    27361 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    27421 tacaatttag
//
