LOCUS       sequence-182460-       13512 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  WDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 
            control (gUbi1+gUbi8+donor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13512)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pJG493
FEATURES             Location/Qualifiers
     source          1..13512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2848..2872
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_RNA        3778..3853
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        4247..4322
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             5094..5147
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5148..5150,5151..5153,5154..5867)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(5193..5214)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5454..5473)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5801..5822
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      5898..6150
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8742..8766
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10067..10696
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11125..12198
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12264..12458
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12694..12716)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12802..12942
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12857..12876
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12957..12974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(13128..13512,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13208..13227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gctgagagat
     1141 cccctcataa tttccccaaa gcgtaaccat gtgtgaataa attttgagct agtagggttg
     1201 cagccacgag taagtcttcc cttgttattg tgtagccaga atgccgcaaa acttccatgc
     1261 ctaagcgaac tgttgagagt acgtttcgat ttctgactgt gttagcctgg aagtgcttgt
     1321 cccaaccttg tttctgagca tgaacgcccg caagccaaca tgttagttga agcatcaggg
     1381 cgattagcag catgatatca aaacgctctg agctgctcgt tcggctatgg cgtaggccta
     1441 gtccgtaggc aggacttttc aagtctcgga aggtttcttc aatctgcatt cgcttcgaat
     1501 agatattaac aagttgtttg ggtgttcgaa tttcaacagg taagttagtt gctagaaccc
     1561 atggctcctt tgccgacgct gagtagattt taggtgacgg gtggtgacaa tgagtccgtg
     1621 tcgagcgctg attttttcgg cctttagagc gagatttata caatagaatt tggcatgaga
     1681 ttggattgct tttagtcagc ctcttatagc ctaaagtctt tgagtgacta gatgacatat
     1741 catgtaagtt gctgataggt ttccagtttt ccgctcctag gtctgcatat tgtacttttc
     1801 ctcttactcg acttaaccag taccaaccca gcttctcaac ggatttatac catggcactt
     1861 taaagccagc atcactgaca atgagcggtg tggtgttact cggtagaatg ctcgcaaggt
     1921 cggctagaaa ttggtcatga gctttctttg aacattgctc tgaaagcggg aacgctttct
     1981 cataaagagt aacagaacga ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt
     2041 tttgctcacg aatatcagac cagtcaacaa gtacaatggg catcgtattg cccgaacaga
     2101 taaagctagc atgccaacgg tatacagcga gtcgctcttt gtggaggtga cgattaccta
     2161 acaatcggtc gattcgtttg atgttatgtt ttgttctcgc tttggttggc aggttacggc
     2221 caagttcggt aagagtgaga gttttacagt caagtaatgc gtggcaagcc aacgttaagc
     2281 tgttgagtcg ttttaagtgt aattcggggc agaattggta aagagagtcg tgtaaaatat
     2341 cgagttcgca catcttgttg tctgattatt gatttttcgc gaaaccattt gatcatatga
     2401 caagatgtgt atccacctta acttaatgat ttttaccaaa atcattaggg gattcatcag
     2461 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     2521 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     2581 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     2641 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     2701 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     2761 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     2821 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     2881 gcttagacaa cttaataaca cattgcggac gtttttaatg tagagctcgt tcctgcggcc
     2941 gcttaattaa ggtagtgaac agaagtccgg caggtcctta gcgaaaaaac ggggtgtgcc
     3001 agaaaactct atcctctacc ctgcgtggag gtgtgaattc tgcacactgc aaatgcaatg
     3061 tgtccaatgc tttatatagg gcaggttttg gcgggagaac agggccctag tgttcccacg
     3121 gtagcgtagc gaatcgtgtg ggccctgttc ggtgtgcggt cggggggcct ccacgcgggt
     3181 tataatatta ccccgcgtgg tggcccccga cgcgcactcg gcttttcgtg agtgcgcgga
     3241 ggcttttgga ccacatcttt tctgatcact ttcgtggaag atgttgattt atcacacttt
     3301 tgacggggaa atctgtgcca tgccttagct tataaggaag tgcgtggtag cccatctcga
     3361 caagtttgta ccgatcgcgg ccgcgatatc ggacgaccaa gcccgttatt ctgacagttc
     3421 tggtgctcaa cacatttata tttatcaagg agcacattgt tactcactgc taggagggaa
     3481 tcgaactagg aatattgatc agaggaacta cgagagagct gaagataact gccctctagc
     3541 tctcactgat ctgggtcgca tagtgagatg cagcccacgt gagttcagca acggtctagc
     3601 gctgggcttt taggcccgca tgatcgggct tttgtcgggt ggtcgacgtg ttcacgattg
     3661 gggagagcaa cgcagcagtt cctcttagtt tagtcccacc tcgcctgtcc agcagagttc
     3721 tgaccggttt ataaactcgc ttgctgcatc agacttgcca gccctgggac tagcagcgtt
     3781 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     3841 accgagtcgg tgcttttttt ccgggaccaa gcccgttatt ctgacagttc tggtgctcaa
     3901 cacatttata tttatcaagg agcacattgt tactcactgc taggagggaa tcgaactagg
     3961 aatattgatc agaggaacta cgagagagct gaagataact gccctctagc tctcactgat
     4021 ctgggtcgca tagtgagatg cagcccacgt gagttcagca acggtctagc gctgggcttt
     4081 taggcccgca tgatcgggct tttgtcgggt ggtcgacgtg ttcacgattg gggagagcaa
     4141 cgcagcagtt cctcttagtt tagtcccacc tcgcctgtcc agcagagttc tgaccggttt
     4201 ataaactcgc ttgctgcatc agacttgctg gtgcaactgg tggcccgttt tagagctaga
     4261 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     4321 gctttttttc gcgtagtcct cggtatggtg ctactggagc tgctagtggc aggccagcag
     4381 gtttatttgg ggctggactt ccggaattag atcaaatgca gcaacagttg agccagaatc
     4441 ccaaccttat gagggagata atgaacatgc caatgatgca gagtctcatg aataaccctg
     4501 atctaatacg caatatgatt atgaataatc cacaaatgcg tgatattatt gatcggaatc
     4561 cagatcttgc ccatgtcctc aatgatccta gtgttctccg ccagaccctt gaagctgcaa
     4621 gaaaccctga aattatgagg gagatgatgc ggaacacaga cagagcaatg agcaacatcg
     4681 aagcttcccc tgaagggttt aatatgctcc ggcgtatgta tgaaactgta caggagcctt
     4741 ttcttaatgc aacaacaatg ggagggggtg gggaaggcac cccggcctct aacccgtttg
     4801 cagctcttct tggaaatcag gggcctaacc aagccggcaa tgctccaact accggcccag
     4861 agtccacaac aggaacccct gttccaaata ctaatccact tccaaacccc tggagcaaca
     4921 atggtaggtt ctagttattt agagtttttt gtttgttttg ttgttgaatg ttgataatta
     4981 catgtggtag tatttttatt ctcacagctg ctgataattg cctgtgatac tattatattt
     5041 tcccagctgg gggtgcgcaa ggaacaacac ggtcaggtcc tgctgctagt ccagagggca
     5101 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cgggcccatg gtgagcaagg
     5161 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     5221 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     5281 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     5341 tcacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     5401 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     5461 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     5521 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     5581 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     5641 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     5701 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     5761 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     5821 tgaccgccgc cgggatcact cacggcatgg acgagctgta caagtaaagc ggccgggtac
     5881 cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     5941 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     6001 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     6061 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     6121 cgcgcgcggt gtcatctatg ttactagatc gcagggctgg tgcaactggt ggcccaccag
     6181 ggctgggttc agcagatttg agcagcctgc tcggtggtct tggtgggaat gcaagaactg
     6241 gtgctgcagg tggtctagga gggttgggtt cagcagattt ggggagtatg cttggtggtc
     6301 cacctgatgc tgctcttttg agtcagatgc tgcaaaaccc tgctatgatg cagatgatgc
     6361 agaacattat gtctgaccca cagtcaatga accaggtcca atatttttca aaactagttc
     6421 ttttatgatt tttggagatg accttggatc attctgtaac atttgcttgt cccacagttg
     6481 cttagcatga acccaaatgc acgtagcctg atggagtcaa acactcagtt gagggatatg
     6541 ttccaaaacc cagaatttct tcgccagatg gcatccccag aggctttgca ggtaaaatct
     6601 gttgtgatgc aagttaacaa ctgttctcgt attttatttt ctgataaaat ttgtatttgt
     6661 tctgcgcagc aattactctc attccagcag acactgtcat cacagcttgg ccaaaatcaa
     6721 cctagccagt gagtaactct tttttttgcg agaaaaaagg gaaaaagtaa cactctaatt
     6781 caatagcatg attgtatcac cccttttttt tatgaaatta aataaaatag agattatgaa
     6841 gtgcagttat gtttatcttt tgagggtgca attatgcgtt tgctgagtct tttcttttca
     6901 gggctggtaa cctagggggc aatggagtgt acttcaagtc acaccggcga gtgtttgatc
     6961 gccggcggta caaagtggtt aaaataatat tttatttatc tcatgtcatt cgattacaga
     7021 ggctcggcta cgagcaaaga caaaccaaat ataacaaaca acaaccctta cacaatgaca
     7081 tcggaaaacg aaatacaaca ccctgagata ttacatttat agaaactgta cgccgtccgc
     7141 gctaggacag tcactgcgaa gcagtgacgt cttcgccgga ggcgaacgag tagttgatga
     7201 acgtctcgcc ttcatacatg tagtgaacaa cagtgttaga gtacatgtaa tccgactgtt
     7261 cgggagtcat atccttgagc caatcttcgt ctggattaac taaaatgatg caaggtattc
     7321 caccccgtat gacctttcgc ttaccatatt ttggattgac cgtgaagtca cgctgagccc
     7381 cgacgaagca cttccagttg ggtgtgaact tgaatggaat gtcgtcgatg atattatact
     7441 tggcgttgac gtcatatgtt gtgaaatcaa ctagactgtt ataataattg tgtgtcccta
     7501 gagaccttgc ccaggaagtc tttcctgttc tggttggccc gcagatgtag atggacttat
     7561 gcctccccgg tgactcctgg aataatcgtc catccactct aagtcagatt gcgcttgatc
     7621 cgcaggagtg gaagtacaaa ggatatagga ttcgaggctt acggagtaga gatgttcatt
     7681 tttccagctt tcaatggtct catggcaaat gagtgattcg gttggaaact caggtgtgta
     7741 agtggcaact gggtcaggaa atagatggcg tgccgtgtac tcgaagtctt tgagacggat
     7801 agaccattca aacggaaaac gattgcaaac catgctgagg aattcctcgc gagaggaact
     7861 agattcaatg atctgtttca tatccgcatc acggtcttta cgacctggag ttgaaacagc
     7921 cacgaatgtt ccccactcag ctgtgtttac atcggagtca acctccttcg tgatgtaatc
     7981 acgaacttgg ttgcagtctt tggcagcttg tatatttgga tggaatatgg agaatggaga
     8041 tgtatccata cggaggttta aggcattggg attggtgatg gaagcacgaa gcttgttctg
     8101 cacgagaacg tgcagatgtg gtgatccatc ttcgtggagc tctctaacag cagcgatgta
     8161 gaggggctca tatttgttca agagagtgcg aagtgaatcc aaggcgtact gtggctcaag
     8221 ggtacattga ggatatgtta gaaagaggta cttggaatag acacggaacc tgggtgcaga
     8281 tgaagaggcc atggtagtga acagaagtcc ggcaggtcct tagcgaaaaa acggggtgtg
     8341 ccagaaaact ctatcctcta ccctgcgtgg aggtgtgaat tctgcacact gcaaatgcaa
     8401 tgtgtccaat gctttatata gggcaggttt tggcgggaga acagggccct agtgttccca
     8461 cggtagcgta gcgaatcgtg tgggccctgt tcggtgtgcg gtcggggggc ctccacgcgg
     8521 gttataatat taccccgcgt ggtggccccc gacgcgcact cggcttttcg tgagtgcgcg
     8581 gaggcttttg gaccacatct tttctgatca ctttcgtgga agatgttgat ttatcacact
     8641 tttgacgggg aaatctgtgc catgccttag cttataagga agtgcgtggt agcccatctc
     8701 ggggccctcg attcgacgtt cctgtttaaa ctatcagtgt ttgacaggat atattggcgg
     8761 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
     8821 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     8881 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     8941 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     9001 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     9061 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     9121 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     9181 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     9241 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     9301 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     9361 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     9421 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     9481 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     9541 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     9601 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     9661 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     9721 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     9781 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     9841 ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg gctgcatgaa atcctggccg
     9901 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     9961 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
    10021 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
    10081 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
    10141 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
    10201 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
    10261 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
    10321 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
    10381 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
    10441 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
    10501 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
    10561 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
    10621 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
    10681 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
    10741 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
    10801 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
    10861 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
    10921 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
    10981 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
    11041 aacgggcggt tggccaggcg taagcggctg ggttgcctgc cggccctgca atggcactgg
    11101 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
    11161 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
    11221 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
    11281 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
    11341 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
    11401 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
    11461 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
    11521 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
    11581 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
    11641 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
    11701 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
    11761 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
    11821 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
    11881 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
    11941 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
    12001 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
    12061 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
    12121 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
    12181 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
    12241 aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
    12301 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
    12361 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
    12421 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
    12481 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
    12541 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
    12601 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
    12661 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggaaac
    12721 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
    12781 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
    12841 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    12901 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
    12961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    13021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    13081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    13141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    13201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    13261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    13321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    13381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    13441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    13501 agagcgaggt at
//
