LOCUS       sequence-182462-        8543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 
            and AtU6 promoters).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8543)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC363
FEATURES             Location/Qualifiers
     source          1..8543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1408..1753
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1696..1716
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1787..5890
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1787..5890
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5903..5923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5934..6182
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6671..6746
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        7232..7307
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             7743..8534
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc aaaactatca ggtgacgtac ccaaagctct ggaagttctc
      901 ggcgcgccga tcggcgcgcc agatttgcct tttcaatttc agaaagaatg ctaacccaca
      961 gatggttaga gaggcttacg cagcaggtat catcaagacg atctacccga gcaataatct
     1021 ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
     1081 ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg
     1141 gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa
     1201 ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga
     1261 actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa
     1321 gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga
     1381 tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa
     1441 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
     1501 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
     1561 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     1621 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     1681 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1741 tttggagaga acacggggga ctcctgcagg tagatcgctc gtcgacatgg ataagaagta
     1801 ctctatcgga ctcgatatcg gaactaactc tgtgggatgg gctgtgatca ccgatgagta
     1861 caaggtgcca tctaagaagt tcaaggttct cggaaacacc gataggcact ctatcaagaa
     1921 aaaccttatc ggtgctctcc tcttcgattc tggtgaaact gctgaggcta ccagactcaa
     1981 gagaaccgct agaagaaggt acaccagaag aaagaacagg atctgctacc tccaagagat
     2041 cttctctaac gagatggcta aagtggatga ttcattcttc cacaggctcg aagagtcatt
     2101 cctcgtggaa gaagataaga agcacgagag gcaccctatc ttcggaaaca tcgttgatga
     2161 ggtggcatac cacgagaagt accctactat ctaccacctc agaaagaagc tcgttgattc
     2221 tactgataag gctgatctca ggctcatcta cctcgctctc gctcacatga tcaagttcag
     2281 aggacacttc ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg ataagttgtt
     2341 catccagctc gtgcagacct acaaccagct tttcgaagag aaccctatca acgcttcagg
     2401 tgtggatgct aaggctatcc tctctgctag gctctctaag tcaagaaggc ttgagaacct
     2461 cattgctcag ctccctggtg agaagaagaa cggacttttc ggaaacttga tcgctctctc
     2521 tctcggactc acccctaact tcaagtctaa cttcgatctc gctgaggatg caaagctcca
     2581 gctctcaaag gatacctacg atgatgatct cgataacctc ctcgctcaga tcggagatca
     2641 gtacgctgat ttgttcctcg ctgctaagaa cctctctgat gctatcctcc tcagtgatat
     2701 cctcagagtg aacaccgaga tcaccaaggc tccactctca gcttctatga tcaagagata
     2761 cgatgagcac caccaggatc tcacacttct caaggctctt gttagacagc agctcccaga
     2821 gaagtacaaa gagattttct tcgatcagtc taagaacgga tacgctggtt acatcgatgg
     2881 tggtgcatct caagaagagt tctacaagtt catcaagcct atcctcgaga agatggatgg
     2941 aaccgaggaa ctcctcgtga agctcaatag agaggatctt ctcagaaagc agaggacctt
     3001 cgataacgga tctatccctc atcagatcca cctcggagag ttgcacgcta tccttagaag
     3061 gcaagaggat ttctacccat tcctcaagga taacagggaa aagattgaga agattctcac
     3121 cttcagaatc ccttactacg tgggacctct cgctagagga aactcaagat tcgcttggat
     3181 gaccagaaag tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg tggataaggg
     3241 tgctagtgct cagtctttca tcgagaggat gaccaacttc gataagaacc ttccaaacga
     3301 gaaggtgctc cctaagcact ctttgctcta cgagtacttc accgtgtaca acgagttgac
     3361 caaggttaag tacgtgaccg agggaatgag gaagcctgct tttttgtcag gtgagcaaaa
     3421 gaaggctatc gttgatctct tgttcaagac caacagaaag gtgaccgtga agcagctcaa
     3481 agaggattac ttcaagaaaa tcgagtgctt cgattcagtt gagatttctg gtgttgagga
     3541 taggttcaac gcatctctcg gaacctacca cgatctcctc aagatcatta aggataagga
     3601 tttcttggat aacgaggaaa acgaggatat cttggaggat atcgttctta ccctcaccct
     3661 ctttgaagat agagagatga ttgaagaaag gctcaagacc tacgctcatc tcttcgatga
     3721 taaggtgatg aagcagttga agagaagaag atacactggt tggggaaggc tctcaagaaa
     3781 gctcattaac ggaatcaggg ataagcagtc tggaaagaca atccttgatt tcctcaagtc
     3841 tgatggattc gctaacagaa acttcatgca gctcatccac gatgattctc tcacctttaa
     3901 agaggatatc cagaaggctc aggtttcagg acagggtgat agtctccatg agcatatcgc
     3961 taacctcgct ggatctcctg caatcaagaa gggaatcctc cagactgtga aggttgtgga
     4021 tgagttggtg aaggtgatgg gaaggcataa gcctgagaac atcgtgatcg aaatggctag
     4081 agagaaccag accactcaga agggacagaa gaactctagg gaaaggatga agaggatcga
     4141 ggaaggtatc aaagagcttg gatctcagat cctcaaagag caccctgttg agaacactca
     4201 gctccagaat gagaagctct acctctacta cctccagaac ggaagggata tgtatgtgga
     4261 tcaagagttg gatatcaaca ggctctctga ttacgatgtt gatcatatcg tgccacagtc
     4321 attcttgaag gatgattcta tcgataacaa ggtgctcacc aggtctgata agaacagggg
     4381 taagagtgat aacgtgccaa gtgaagaggt tgtgaagaaa atgaagaact attggaggca
     4441 gctcctcaac gctaagctca tcactcagag aaagttcgat aacttgacta aggctgagag
     4501 gggaggactc tctgaattgg ataaggcagg attcatcaag aggcagcttg tggaaaccag
     4561 gcagatcact aagcacgttg cacagatcct cgattctagg atgaacacca agtacgatga
     4621 gaacgataag ttgatcaggg aagtgaaggt tatcaccctc aagtcaaagc tcgtgtctga
     4681 tttcagaaag gatttccaat tctacaaggt gagggaaatc aacaactacc accacgctca
     4741 cgatgcttac cttaacgctg ttgttggaac cgctctcatc aagaagtatc ctaagctcga
     4801 gtcagagttc gtgtacggtg attacaaggt gtacgatgtg aggaagatga tcgctaagtc
     4861 tgagcaagag atcggaaagg ctaccgctaa gtatttcttc tactctaaca tcatgaattt
     4921 cttcaagacc gagattaccc tcgctaacgg tgagatcaga aagaggccac tcatcgagac
     4981 aaacggtgaa acaggtgaga tcgtgtggga taagggaagg gatttcgcta ccgttagaaa
     5041 ggtgctctct atgccacagg tgaacatcgt taagaaaacc gaggtgcaga ccggtggatt
     5101 ctctaaagag tctatcctcc ctaagaggaa ctctgataag ctcattgcta ggaagaagga
     5161 ttgggaccct aagaaatacg gtggtttcga ttctcctacc gtggcttact ctgttctcgt
     5221 tgtggctaag gttgagaagg gaaagagtaa gaagctcaag tctgttaagg aacttctcgg
     5281 aatcactatc atggaaaggt catctttcga gaagaaccca atcgatttcc tcgaggctaa
     5341 gggatacaaa gaggttaaga aggatctcat catcaagctc ccaaagtact cactcttcga
     5401 actcgagaac ggtagaaaga ggatgctcgc ttctgctggt gagcttcaaa agggaaacga
     5461 gcttgctctc ccatctaagt acgttaactt tctttacctc gcttctcact acgagaagtt
     5521 gaagggatct ccagaagata acgagcagaa gcaacttttc gttgagcagc acaagcacta
     5581 cttggatgag atcatcgagc agatctctga gttctctaaa agggtgatcc tcgctgatgc
     5641 aaacctcgat aaggtgttgt ctgcttacaa caagcacaga gataagccta tcagggaaca
     5701 ggcagagaac atcatccatc tcttcaccct taccaacctc ggtgctcctg ctgctttcaa
     5761 gtacttcgat acaaccatcg ataggaagag atacacctct accaaagaag tgctcgatgc
     5821 taccctcatc catcagtcta tcactggact ctacgagact aggatcgatc tctcacagct
     5881 cggtggtgat tcaagggctg atcctaagaa gaagaggaag gtttgactcg agatatgaag
     5941 atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt
     6001 ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc
     6061 acattataat gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct
     6121 tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg attaaagata
     6181 aggagctccg gtgacggacc catggcttcg ttgaacaacg gaaactcgac ttgccttccg
     6241 cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca gttttttttt
     6301 gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat
     6361 tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa
     6421 ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa
     6481 aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag
     6541 tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag
     6601 ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt gctgccagcc
     6661 tgatctttga gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     6721 ttgaaaaagt ggcaccgagt cggtgctttt tttcccggcg ccatggcttc gttgaacaac
     6781 ggaaactcga cttgccttcc gcacaataca tcatttcttc ttagcttttt ttcttcttct
     6841 tcgttcatac agtttttttt tgtttatcag cttacatttt cttgaaccgt agctttcgtt
     6901 ttcttctttt taactttcca ttcggagttt ttgtatcttg tttcatagtt tgtcccagga
     6961 ttagaatgat taggcatcga accttcaaga atttgattga ataaaacatc ttcattctta
     7021 agatatgaag ataatcttca aaaggcccct gggaatctga aagaagagaa gcaggcccat
     7081 ttatatggga aagaacaata gtatttctta tataggccca tttaagttga aaacaatctt
     7141 caaaagtccc acatcgctta gataagaaaa cgaagctgag tttatataca gctagagtcg
     7201 aagtagtgat tgtaaagttt tctggttaat tgttttagag ctagaaatag caagttaaaa
     7261 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttcgcgcg
     7321 tagtcctcgg taatatcgca gaacaaaagt acctgatatc gagtgtactt caagtcacac
     7381 cggcgagtgc cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     7441 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     7501 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     7561 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     7621 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     7681 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     7741 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     7801 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     7861 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     7921 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     7981 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     8041 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     8101 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     8161 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     8221 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     8281 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     8341 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     8401 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     8461 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     8521 tagtcggcaa ataaccctcg agc
//
