LOCUS       sequence-182464-        9009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV 
            promoter, Csy4 processing).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9009)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC364
FEATURES             Location/Qualifiers
     source          1..9009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1408..1753
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1696..1716
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2345..2401
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2402..6505
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2402..6505
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6518..6538
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6549..6797
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    7294..7313
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7334..7409
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7410..7429
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     misc_RNA        7450..7525
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    7526..7545
                     /label=Csy4 Site
                     /bound_moiety="Pseudomonas aeruginosa Csy4 (Cas6f)"
                     /note="cleavage site for the Csy4 endoribonuclease (Cermak 
                     et al. 2017)"
     polyA_signal    7565..7739
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             8209..9000
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc aaaactatca ggtgacgtac ccaaagctct ggaagttctc
      901 ggcgcgccga tcggcgcgcc agatttgcct tttcaatttc agaaagaatg ctaacccaca
      961 gatggttaga gaggcttacg cagcaggtat catcaagacg atctacccga gcaataatct
     1021 ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
     1081 ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg
     1141 gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa
     1201 ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga
     1261 actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa
     1321 gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga
     1381 tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa
     1441 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
     1501 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
     1561 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     1621 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     1681 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1741 tttggagaga acacggggga ctcctgcagg aaaaatggat cattatcttg atattagact
     1801 tagacctgat ccagaatttc caccagctca acttatgtct gttctttttg gaaaacttca
     1861 tcaagctctt gttgctcaag gaggagatag aattggagtt tcttttcctg atcttgatga
     1921 atcaagatca agacttggag aaagacttag aattcatgct tctgctgatg atcttagagc
     1981 tttgcttgct agaccttggc ttgaaggact tagagatcat cttcaatttg gagaaccagc
     2041 tgttgttcca catccaactc cttatagaca agtttcaaga gttcaagcta aatctaatcc
     2101 agaaagactt agaagaagac ttatgagaag acatgatctt tctgaagaag aagctagaaa
     2161 aagaattcct gatactgttg ctagagcttt ggatttgcct tttgttacac ttagatcaca
     2221 atctactgga caacatttta gactttttat tagacatgga ccacttcaag ttactgctga
     2281 agaaggagga tttacttgtt atggactttc taagggaggt tttgttcctt ggtttggatc
     2341 tggagctact aatttttctc ttcttaagca agctggagat gttgaagaaa atcctggacc
     2401 catggataag aagtactcta tcggactcga tatcggaact aactctgtgg gatgggctgt
     2461 gatcaccgat gagtacaagg tgccatctaa gaagttcaag gttctcggaa acaccgatag
     2521 gcactctatc aagaaaaacc ttatcggtgc tctcctcttc gattctggtg aaactgctga
     2581 ggctaccaga ctcaagagaa ccgctagaag aaggtacacc agaagaaaga acaggatctg
     2641 ctacctccaa gagatcttct ctaacgagat ggctaaagtg gatgattcat tcttccacag
     2701 gctcgaagag tcattcctcg tggaagaaga taagaagcac gagaggcacc ctatcttcgg
     2761 aaacatcgtt gatgaggtgg cataccacga gaagtaccct actatctacc acctcagaaa
     2821 gaagctcgtt gattctactg ataaggctga tctcaggctc atctacctcg ctctcgctca
     2881 catgatcaag ttcagaggac acttcctcat cgagggtgat ctcaaccctg ataactctga
     2941 tgtggataag ttgttcatcc agctcgtgca gacctacaac cagcttttcg aagagaaccc
     3001 tatcaacgct tcaggtgtgg atgctaaggc tatcctctct gctaggctct ctaagtcaag
     3061 aaggcttgag aacctcattg ctcagctccc tggtgagaag aagaacggac ttttcggaaa
     3121 cttgatcgct ctctctctcg gactcacccc taacttcaag tctaacttcg atctcgctga
     3181 ggatgcaaag ctccagctct caaaggatac ctacgatgat gatctcgata acctcctcgc
     3241 tcagatcgga gatcagtacg ctgatttgtt cctcgctgct aagaacctct ctgatgctat
     3301 cctcctcagt gatatcctca gagtgaacac cgagatcacc aaggctccac tctcagcttc
     3361 tatgatcaag agatacgatg agcaccacca ggatctcaca cttctcaagg ctcttgttag
     3421 acagcagctc ccagagaagt acaaagagat tttcttcgat cagtctaaga acggatacgc
     3481 tggttacatc gatggtggtg catctcaaga agagttctac aagttcatca agcctatcct
     3541 cgagaagatg gatggaaccg aggaactcct cgtgaagctc aatagagagg atcttctcag
     3601 aaagcagagg accttcgata acggatctat ccctcatcag atccacctcg gagagttgca
     3661 cgctatcctt agaaggcaag aggatttcta cccattcctc aaggataaca gggaaaagat
     3721 tgagaagatt ctcaccttca gaatccctta ctacgtggga cctctcgcta gaggaaactc
     3781 aagattcgct tggatgacca gaaagtctga ggaaaccatc accccttgga acttcgaaga
     3841 ggtggtggat aagggtgcta gtgctcagtc tttcatcgag aggatgacca acttcgataa
     3901 gaaccttcca aacgagaagg tgctccctaa gcactctttg ctctacgagt acttcaccgt
     3961 gtacaacgag ttgaccaagg ttaagtacgt gaccgaggga atgaggaagc ctgctttttt
     4021 gtcaggtgag caaaagaagg ctatcgttga tctcttgttc aagaccaaca gaaaggtgac
     4081 cgtgaagcag ctcaaagagg attacttcaa gaaaatcgag tgcttcgatt cagttgagat
     4141 ttctggtgtt gaggataggt tcaacgcatc tctcggaacc taccacgatc tcctcaagat
     4201 cattaaggat aaggatttct tggataacga ggaaaacgag gatatcttgg aggatatcgt
     4261 tcttaccctc accctctttg aagatagaga gatgattgaa gaaaggctca agacctacgc
     4321 tcatctcttc gatgataagg tgatgaagca gttgaagaga agaagataca ctggttgggg
     4381 aaggctctca agaaagctca ttaacggaat cagggataag cagtctggaa agacaatcct
     4441 tgatttcctc aagtctgatg gattcgctaa cagaaacttc atgcagctca tccacgatga
     4501 ttctctcacc tttaaagagg atatccagaa ggctcaggtt tcaggacagg gtgatagtct
     4561 ccatgagcat atcgctaacc tcgctggatc tcctgcaatc aagaagggaa tcctccagac
     4621 tgtgaaggtt gtggatgagt tggtgaaggt gatgggaagg cataagcctg agaacatcgt
     4681 gatcgaaatg gctagagaga accagaccac tcagaaggga cagaagaact ctagggaaag
     4741 gatgaagagg atcgaggaag gtatcaaaga gcttggatct cagatcctca aagagcaccc
     4801 tgttgagaac actcagctcc agaatgagaa gctctacctc tactacctcc agaacggaag
     4861 ggatatgtat gtggatcaag agttggatat caacaggctc tctgattacg atgttgatca
     4921 tatcgtgcca cagtcattct tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc
     4981 tgataagaac aggggtaaga gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa
     5041 gaactattgg aggcagctcc tcaacgctaa gctcatcact cagagaaagt tcgataactt
     5101 gactaaggct gagaggggag gactctctga attggataag gcaggattca tcaagaggca
     5161 gcttgtggaa accaggcaga tcactaagca cgttgcacag atcctcgatt ctaggatgaa
     5221 caccaagtac gatgagaacg ataagttgat cagggaagtg aaggttatca ccctcaagtc
     5281 aaagctcgtg tctgatttca gaaaggattt ccaattctac aaggtgaggg aaatcaacaa
     5341 ctaccaccac gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa
     5401 gtatcctaag ctcgagtcag agttcgtgta cggtgattac aaggtgtacg atgtgaggaa
     5461 gatgatcgct aagtctgagc aagagatcgg aaaggctacc gctaagtatt tcttctactc
     5521 taacatcatg aatttcttca agaccgagat taccctcgct aacggtgaga tcagaaagag
     5581 gccactcatc gagacaaacg gtgaaacagg tgagatcgtg tgggataagg gaagggattt
     5641 cgctaccgtt agaaaggtgc tctctatgcc acaggtgaac atcgttaaga aaaccgaggt
     5701 gcagaccggt ggattctcta aagagtctat cctccctaag aggaactctg ataagctcat
     5761 tgctaggaag aaggattggg accctaagaa atacggtggt ttcgattctc ctaccgtggc
     5821 ttactctgtt ctcgttgtgg ctaaggttga gaagggaaag agtaagaagc tcaagtctgt
     5881 taaggaactt ctcggaatca ctatcatgga aaggtcatct ttcgagaaga acccaatcga
     5941 tttcctcgag gctaagggat acaaagaggt taagaaggat ctcatcatca agctcccaaa
     6001 gtactcactc ttcgaactcg agaacggtag aaagaggatg ctcgcttctg ctggtgagct
     6061 tcaaaaggga aacgagcttg ctctcccatc taagtacgtt aactttcttt acctcgcttc
     6121 tcactacgag aagttgaagg gatctccaga agataacgag cagaagcaac ttttcgttga
     6181 gcagcacaag cactacttgg atgagatcat cgagcagatc tctgagttct ctaaaagggt
     6241 gatcctcgct gatgcaaacc tcgataaggt gttgtctgct tacaacaagc acagagataa
     6301 gcctatcagg gaacaggcag agaacatcat ccatctcttc acccttacca acctcggtgc
     6361 tcctgctgct ttcaagtact tcgatacaac catcgatagg aagagataca cctctaccaa
     6421 agaagtgctc gatgctaccc tcatccatca gtctatcact ggactctacg agactaggat
     6481 cgatctctca cagctcggtg gtgattcaag ggctgatcct aagaagaaga ggaaggtttg
     6541 actcgagata tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct
     6601 taagtttgtg tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta
     6661 aatgaatgta agatcacatt ataatgaata aacaaatgtt tctataatcc attgtgaatg
     6721 ttttgttgga tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga
     6781 atatgattaa agataaggag ctccggtgac ggacggcgcg ctggcagaca tactgtccca
     6841 caaatgaaga tggaatctgt aaaagaaaac gcgtgaaata atgcgtctga caaaggttag
     6901 gtcggctgcc tttaatcaat accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc
     6961 ggtttggctt tttctgacga acaaataaga ttcgtggccg acaggtgggg gtccaccatg
     7021 tgaaggcatc ttcagactcc aataatggag caatgacgta agggcttacg aaataagtaa
     7081 gggtagtttg ggaaatgtcc actcacccgt cagtctataa atacttagcc cctccctcat
     7141 tgttaaggga gcaaaatctc agagagatag tcctagagag agaaagagag caagtagcct
     7201 agaagtagtc aaggcggcga agtattcagg cacgtggcca ggaagaagaa aagccaagac
     7261 gacgaaaaca ggtaagagct aagcttcctg caggttcact gccgtatagg caggctgcca
     7321 gcctgatctt tgagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     7381 aacttgaaaa agtggcaccg agtcggtgcg ttcactgccg tataggcagg taaagttttc
     7441 tggttaattg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
     7501 tgaaaaagtg gcaccgagtc ggtgcgttca ctgccgtata ggcaggtcga tcgacaagct
     7561 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
     7621 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
     7681 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc
     7741 cccgaattac cggcgagctt tgggtacgtc acgtggctcg agcgcgtagt cctcggtaat
     7801 atcgcagaac aaaagtacct gatatcgagt gtacttcaag tcacaccggc gagtgccagc
     7861 caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg
     7921 aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
     7981 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     8041 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
     8101 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     8161 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg
     8221 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     8281 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     8341 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     8401 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     8461 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     8521 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     8581 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     8641 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     8701 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     8761 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     8821 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     8881 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     8941 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
     9001 ccctcgagc
//
