LOCUS       sequence-182465-        8570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV 
            promoter, tRNA processing).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC365
FEATURES             Location/Qualifiers
     source          1..8570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1412..1757
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1700..1720
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1791..5894
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1791..5894
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5907..5927
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5938..6186
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6781..6856
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        6954..7029
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    7126..7300
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             7770..8561
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc atatgcaact atcaggtgac gtacccaaag ctctggaagt
      901 tctcggcgcg ccgatcggcg cgccagattt gccttttcaa tttcagaaag aatgctaacc
      961 cacagatggt tagagaggct tacgcagcag gtatcatcaa gacgatctac ccgagcaata
     1021 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
     1081 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
     1141 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
     1201 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
     1261 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
     1321 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
     1381 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     1441 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     1501 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1561 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1621 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1681 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1741 ttcatttgga gagaacacgg gggactcctg caggtagatc gctcgtcgac atggataaga
     1801 agtactctat cggactcgat atcggaacta actctgtggg atgggctgtg atcaccgatg
     1861 agtacaaggt gccatctaag aagttcaagg ttctcggaaa caccgatagg cactctatca
     1921 agaaaaacct tatcggtgct ctcctcttcg attctggtga aactgctgag gctaccagac
     1981 tcaagagaac cgctagaaga aggtacacca gaagaaagaa caggatctgc tacctccaag
     2041 agatcttctc taacgagatg gctaaagtgg atgattcatt cttccacagg ctcgaagagt
     2101 cattcctcgt ggaagaagat aagaagcacg agaggcaccc tatcttcgga aacatcgttg
     2161 atgaggtggc ataccacgag aagtacccta ctatctacca cctcagaaag aagctcgttg
     2221 attctactga taaggctgat ctcaggctca tctacctcgc tctcgctcac atgatcaagt
     2281 tcagaggaca cttcctcatc gagggtgatc tcaaccctga taactctgat gtggataagt
     2341 tgttcatcca gctcgtgcag acctacaacc agcttttcga agagaaccct atcaacgctt
     2401 caggtgtgga tgctaaggct atcctctctg ctaggctctc taagtcaaga aggcttgaga
     2461 acctcattgc tcagctccct ggtgagaaga agaacggact tttcggaaac ttgatcgctc
     2521 tctctctcgg actcacccct aacttcaagt ctaacttcga tctcgctgag gatgcaaagc
     2581 tccagctctc aaaggatacc tacgatgatg atctcgataa cctcctcgct cagatcggag
     2641 atcagtacgc tgatttgttc ctcgctgcta agaacctctc tgatgctatc ctcctcagtg
     2701 atatcctcag agtgaacacc gagatcacca aggctccact ctcagcttct atgatcaaga
     2761 gatacgatga gcaccaccag gatctcacac ttctcaaggc tcttgttaga cagcagctcc
     2821 cagagaagta caaagagatt ttcttcgatc agtctaagaa cggatacgct ggttacatcg
     2881 atggtggtgc atctcaagaa gagttctaca agttcatcaa gcctatcctc gagaagatgg
     2941 atggaaccga ggaactcctc gtgaagctca atagagagga tcttctcaga aagcagagga
     3001 ccttcgataa cggatctatc cctcatcaga tccacctcgg agagttgcac gctatcctta
     3061 gaaggcaaga ggatttctac ccattcctca aggataacag ggaaaagatt gagaagattc
     3121 tcaccttcag aatcccttac tacgtgggac ctctcgctag aggaaactca agattcgctt
     3181 ggatgaccag aaagtctgag gaaaccatca ccccttggaa cttcgaagag gtggtggata
     3241 agggtgctag tgctcagtct ttcatcgaga ggatgaccaa cttcgataag aaccttccaa
     3301 acgagaaggt gctccctaag cactctttgc tctacgagta cttcaccgtg tacaacgagt
     3361 tgaccaaggt taagtacgtg accgagggaa tgaggaagcc tgcttttttg tcaggtgagc
     3421 aaaagaaggc tatcgttgat ctcttgttca agaccaacag aaaggtgacc gtgaagcagc
     3481 tcaaagagga ttacttcaag aaaatcgagt gcttcgattc agttgagatt tctggtgttg
     3541 aggataggtt caacgcatct ctcggaacct accacgatct cctcaagatc attaaggata
     3601 aggatttctt ggataacgag gaaaacgagg atatcttgga ggatatcgtt cttaccctca
     3661 ccctctttga agatagagag atgattgaag aaaggctcaa gacctacgct catctcttcg
     3721 atgataaggt gatgaagcag ttgaagagaa gaagatacac tggttgggga aggctctcaa
     3781 gaaagctcat taacggaatc agggataagc agtctggaaa gacaatcctt gatttcctca
     3841 agtctgatgg attcgctaac agaaacttca tgcagctcat ccacgatgat tctctcacct
     3901 ttaaagagga tatccagaag gctcaggttt caggacaggg tgatagtctc catgagcata
     3961 tcgctaacct cgctggatct cctgcaatca agaagggaat cctccagact gtgaaggttg
     4021 tggatgagtt ggtgaaggtg atgggaaggc ataagcctga gaacatcgtg atcgaaatgg
     4081 ctagagagaa ccagaccact cagaagggac agaagaactc tagggaaagg atgaagagga
     4141 tcgaggaagg tatcaaagag cttggatctc agatcctcaa agagcaccct gttgagaaca
     4201 ctcagctcca gaatgagaag ctctacctct actacctcca gaacggaagg gatatgtatg
     4261 tggatcaaga gttggatatc aacaggctct ctgattacga tgttgatcat atcgtgccac
     4321 agtcattctt gaaggatgat tctatcgata acaaggtgct caccaggtct gataagaaca
     4381 ggggtaagag tgataacgtg ccaagtgaag aggttgtgaa gaaaatgaag aactattgga
     4441 ggcagctcct caacgctaag ctcatcactc agagaaagtt cgataacttg actaaggctg
     4501 agaggggagg actctctgaa ttggataagg caggattcat caagaggcag cttgtggaaa
     4561 ccaggcagat cactaagcac gttgcacaga tcctcgattc taggatgaac accaagtacg
     4621 atgagaacga taagttgatc agggaagtga aggttatcac cctcaagtca aagctcgtgt
     4681 ctgatttcag aaaggatttc caattctaca aggtgaggga aatcaacaac taccaccacg
     4741 ctcacgatgc ttaccttaac gctgttgttg gaaccgctct catcaagaag tatcctaagc
     4801 tcgagtcaga gttcgtgtac ggtgattaca aggtgtacga tgtgaggaag atgatcgcta
     4861 agtctgagca agagatcgga aaggctaccg ctaagtattt cttctactct aacatcatga
     4921 atttcttcaa gaccgagatt accctcgcta acggtgagat cagaaagagg ccactcatcg
     4981 agacaaacgg tgaaacaggt gagatcgtgt gggataaggg aagggatttc gctaccgtta
     5041 gaaaggtgct ctctatgcca caggtgaaca tcgttaagaa aaccgaggtg cagaccggtg
     5101 gattctctaa agagtctatc ctccctaaga ggaactctga taagctcatt gctaggaaga
     5161 aggattggga ccctaagaaa tacggtggtt tcgattctcc taccgtggct tactctgttc
     5221 tcgttgtggc taaggttgag aagggaaaga gtaagaagct caagtctgtt aaggaacttc
     5281 tcggaatcac tatcatggaa aggtcatctt tcgagaagaa cccaatcgat ttcctcgagg
     5341 ctaagggata caaagaggtt aagaaggatc tcatcatcaa gctcccaaag tactcactct
     5401 tcgaactcga gaacggtaga aagaggatgc tcgcttctgc tggtgagctt caaaagggaa
     5461 acgagcttgc tctcccatct aagtacgtta actttcttta cctcgcttct cactacgaga
     5521 agttgaaggg atctccagaa gataacgagc agaagcaact tttcgttgag cagcacaagc
     5581 actacttgga tgagatcatc gagcagatct ctgagttctc taaaagggtg atcctcgctg
     5641 atgcaaacct cgataaggtg ttgtctgctt acaacaagca cagagataag cctatcaggg
     5701 aacaggcaga gaacatcatc catctcttca cccttaccaa cctcggtgct cctgctgctt
     5761 tcaagtactt cgatacaacc atcgatagga agagatacac ctctaccaaa gaagtgctcg
     5821 atgctaccct catccatcag tctatcactg gactctacga gactaggatc gatctctcac
     5881 agctcggtgg tgattcaagg gctgatccta agaagaagag gaaggtttga ctcgagatat
     5941 gaagatgaag atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt
     6001 ttttttcttg gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa
     6061 gatcacatta taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat
     6121 ctcttctgca gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa
     6181 gataaggagc tccggtgacg gacggcgcgc tggcagacat actgtcccac aaatgaagat
     6241 ggaatctgta aaagaaaacg cgtgaaataa tgcgtctgac aaaggttagg tcggctgcct
     6301 ttaatcaata ccaaagtggt ccctaccacg atggaaaaac tgtgcagtcg gtttggcttt
     6361 ttctgacgaa caaataagat tcgtggccga caggtggggg tccaccatgt gaaggcatct
     6421 tcagactcca ataatggagc aatgacgtaa gggcttacga aataagtaag ggtagtttgg
     6481 gaaatgtcca ctcacccgtc agtctataaa tacttagccc ctccctcatt gttaagggag
     6541 caaaatctca gagagatagt cctagagaga gaaagagagc aagtagccta gaagtagtca
     6601 aggcggcgaa gtattcaggc acgtggccag gaagaagaaa agccaagacg acgaaaacag
     6661 gtaagagcta agcttcctgc aggaacaaag caccagtggt ctagtggtag aatagtaccc
     6721 tgccacggta cagacccggg ttcgattccc ggctggtgca gctgccagcc tgatctttga
     6781 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
     6841 ggcaccgagt cggtgcaaca aagcaccagt ggtctagtgg tagaatagta ccctgccacg
     6901 gtacagaccc gggttcgatt cccggctggt gcagtaaagt tttctggtta attgttttag
     6961 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     7021 agtcggtgca acaaagcacc agtggtctag tggtagaata gtaccctgcc acggtacaga
     7081 cccgggttcg attcccggct ggtgcagtcg atcgacaagc tcgagtttct ccataataat
     7141 gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga
     7201 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     7261 ctaattccta aaaccaaaat ccagtactaa aatccagatc ccccgaatta ccggcgagct
     7321 ttgggtacgt cacgtggctc gagcgcgtag tcctcggtaa tatcgcagaa caaaagtacc
     7381 tgatatcgag tgtacttcaa gtcacaccgg cgagtgccag ccaggacaga aatgcctcga
     7441 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa
     7501 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     7561 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     7621 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     7681 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     7741 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga
     7801 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     7861 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     7921 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     7981 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     8041 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     8101 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     8161 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     8221 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     8281 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     8341 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     8401 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     8461 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     8521 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc
//
