LOCUS       sequence-182466-        8419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV 
            promoter, ribozyme processing).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTC366
FEATURES             Location/Qualifiers
     source          1..8419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     573..592
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     826..843
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1408..1753
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1696..1716
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1787..5890
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             1787..5890
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5903..5923
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5934..6182
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        6715..6790
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        6824..6899
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    6975..7149
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             7619..8410
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
      781 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      841 gaggaagcgg gagagcgccc aaaactatca ggtgacgtac ccaaagctct ggaagttctc
      901 ggcgcgccga tcggcgcgcc agatttgcct tttcaatttc agaaagaatg ctaacccaca
      961 gatggttaga gaggcttacg cagcaggtat catcaagacg atctacccga gcaataatct
     1021 ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
     1081 ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg
     1141 gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa
     1201 ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga
     1261 actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa
     1321 gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga
     1381 tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa
     1441 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
     1501 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
     1561 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     1621 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     1681 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1741 tttggagaga acacggggga ctcctgcagg tagatcgctc gtcgacatgg ataagaagta
     1801 ctctatcgga ctcgatatcg gaactaactc tgtgggatgg gctgtgatca ccgatgagta
     1861 caaggtgcca tctaagaagt tcaaggttct cggaaacacc gataggcact ctatcaagaa
     1921 aaaccttatc ggtgctctcc tcttcgattc tggtgaaact gctgaggcta ccagactcaa
     1981 gagaaccgct agaagaaggt acaccagaag aaagaacagg atctgctacc tccaagagat
     2041 cttctctaac gagatggcta aagtggatga ttcattcttc cacaggctcg aagagtcatt
     2101 cctcgtggaa gaagataaga agcacgagag gcaccctatc ttcggaaaca tcgttgatga
     2161 ggtggcatac cacgagaagt accctactat ctaccacctc agaaagaagc tcgttgattc
     2221 tactgataag gctgatctca ggctcatcta cctcgctctc gctcacatga tcaagttcag
     2281 aggacacttc ctcatcgagg gtgatctcaa ccctgataac tctgatgtgg ataagttgtt
     2341 catccagctc gtgcagacct acaaccagct tttcgaagag aaccctatca acgcttcagg
     2401 tgtggatgct aaggctatcc tctctgctag gctctctaag tcaagaaggc ttgagaacct
     2461 cattgctcag ctccctggtg agaagaagaa cggacttttc ggaaacttga tcgctctctc
     2521 tctcggactc acccctaact tcaagtctaa cttcgatctc gctgaggatg caaagctcca
     2581 gctctcaaag gatacctacg atgatgatct cgataacctc ctcgctcaga tcggagatca
     2641 gtacgctgat ttgttcctcg ctgctaagaa cctctctgat gctatcctcc tcagtgatat
     2701 cctcagagtg aacaccgaga tcaccaaggc tccactctca gcttctatga tcaagagata
     2761 cgatgagcac caccaggatc tcacacttct caaggctctt gttagacagc agctcccaga
     2821 gaagtacaaa gagattttct tcgatcagtc taagaacgga tacgctggtt acatcgatgg
     2881 tggtgcatct caagaagagt tctacaagtt catcaagcct atcctcgaga agatggatgg
     2941 aaccgaggaa ctcctcgtga agctcaatag agaggatctt ctcagaaagc agaggacctt
     3001 cgataacgga tctatccctc atcagatcca cctcggagag ttgcacgcta tccttagaag
     3061 gcaagaggat ttctacccat tcctcaagga taacagggaa aagattgaga agattctcac
     3121 cttcagaatc ccttactacg tgggacctct cgctagagga aactcaagat tcgcttggat
     3181 gaccagaaag tctgaggaaa ccatcacccc ttggaacttc gaagaggtgg tggataaggg
     3241 tgctagtgct cagtctttca tcgagaggat gaccaacttc gataagaacc ttccaaacga
     3301 gaaggtgctc cctaagcact ctttgctcta cgagtacttc accgtgtaca acgagttgac
     3361 caaggttaag tacgtgaccg agggaatgag gaagcctgct tttttgtcag gtgagcaaaa
     3421 gaaggctatc gttgatctct tgttcaagac caacagaaag gtgaccgtga agcagctcaa
     3481 agaggattac ttcaagaaaa tcgagtgctt cgattcagtt gagatttctg gtgttgagga
     3541 taggttcaac gcatctctcg gaacctacca cgatctcctc aagatcatta aggataagga
     3601 tttcttggat aacgaggaaa acgaggatat cttggaggat atcgttctta ccctcaccct
     3661 ctttgaagat agagagatga ttgaagaaag gctcaagacc tacgctcatc tcttcgatga
     3721 taaggtgatg aagcagttga agagaagaag atacactggt tggggaaggc tctcaagaaa
     3781 gctcattaac ggaatcaggg ataagcagtc tggaaagaca atccttgatt tcctcaagtc
     3841 tgatggattc gctaacagaa acttcatgca gctcatccac gatgattctc tcacctttaa
     3901 agaggatatc cagaaggctc aggtttcagg acagggtgat agtctccatg agcatatcgc
     3961 taacctcgct ggatctcctg caatcaagaa gggaatcctc cagactgtga aggttgtgga
     4021 tgagttggtg aaggtgatgg gaaggcataa gcctgagaac atcgtgatcg aaatggctag
     4081 agagaaccag accactcaga agggacagaa gaactctagg gaaaggatga agaggatcga
     4141 ggaaggtatc aaagagcttg gatctcagat cctcaaagag caccctgttg agaacactca
     4201 gctccagaat gagaagctct acctctacta cctccagaac ggaagggata tgtatgtgga
     4261 tcaagagttg gatatcaaca ggctctctga ttacgatgtt gatcatatcg tgccacagtc
     4321 attcttgaag gatgattcta tcgataacaa ggtgctcacc aggtctgata agaacagggg
     4381 taagagtgat aacgtgccaa gtgaagaggt tgtgaagaaa atgaagaact attggaggca
     4441 gctcctcaac gctaagctca tcactcagag aaagttcgat aacttgacta aggctgagag
     4501 gggaggactc tctgaattgg ataaggcagg attcatcaag aggcagcttg tggaaaccag
     4561 gcagatcact aagcacgttg cacagatcct cgattctagg atgaacacca agtacgatga
     4621 gaacgataag ttgatcaggg aagtgaaggt tatcaccctc aagtcaaagc tcgtgtctga
     4681 tttcagaaag gatttccaat tctacaaggt gagggaaatc aacaactacc accacgctca
     4741 cgatgcttac cttaacgctg ttgttggaac cgctctcatc aagaagtatc ctaagctcga
     4801 gtcagagttc gtgtacggtg attacaaggt gtacgatgtg aggaagatga tcgctaagtc
     4861 tgagcaagag atcggaaagg ctaccgctaa gtatttcttc tactctaaca tcatgaattt
     4921 cttcaagacc gagattaccc tcgctaacgg tgagatcaga aagaggccac tcatcgagac
     4981 aaacggtgaa acaggtgaga tcgtgtggga taagggaagg gatttcgcta ccgttagaaa
     5041 ggtgctctct atgccacagg tgaacatcgt taagaaaacc gaggtgcaga ccggtggatt
     5101 ctctaaagag tctatcctcc ctaagaggaa ctctgataag ctcattgcta ggaagaagga
     5161 ttgggaccct aagaaatacg gtggtttcga ttctcctacc gtggcttact ctgttctcgt
     5221 tgtggctaag gttgagaagg gaaagagtaa gaagctcaag tctgttaagg aacttctcgg
     5281 aatcactatc atggaaaggt catctttcga gaagaaccca atcgatttcc tcgaggctaa
     5341 gggatacaaa gaggttaaga aggatctcat catcaagctc ccaaagtact cactcttcga
     5401 actcgagaac ggtagaaaga ggatgctcgc ttctgctggt gagcttcaaa agggaaacga
     5461 gcttgctctc ccatctaagt acgttaactt tctttacctc gcttctcact acgagaagtt
     5521 gaagggatct ccagaagata acgagcagaa gcaacttttc gttgagcagc acaagcacta
     5581 cttggatgag atcatcgagc agatctctga gttctctaaa agggtgatcc tcgctgatgc
     5641 aaacctcgat aaggtgttgt ctgcttacaa caagcacaga gataagccta tcagggaaca
     5701 ggcagagaac atcatccatc tcttcaccct taccaacctc ggtgctcctg ctgctttcaa
     5761 gtacttcgat acaaccatcg ataggaagag atacacctct accaaagaag tgctcgatgc
     5821 taccctcatc catcagtcta tcactggact ctacgagact aggatcgatc tctcacagct
     5881 cggtggtgat tcaagggctg atcctaagaa gaagaggaag gtttgactcg agatatgaag
     5941 atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt
     6001 ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc
     6061 acattataat gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct
     6121 tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg attaaagata
     6181 aggagctccg gtgacggacg gcgcgctggc agacatactg tcccacaaat gaagatggaa
     6241 tctgtaaaag aaaacgcgtg aaataatgcg tctgacaaag gttaggtcgg ctgcctttaa
     6301 tcaataccaa agtggtccct accacgatgg aaaaactgtg cagtcggttt ggctttttct
     6361 gacgaacaaa taagattcgt ggccgacagg tgggggtcca ccatgtgaag gcatcttcag
     6421 actccaataa tggagcaatg acgtaagggc ttacgaaata agtaagggta gtttgggaaa
     6481 tgtccactca cccgtcagtc tataaatact tagcccctcc ctcattgtta agggagcaaa
     6541 atctcagaga gatagtccta gagagagaaa gagagcaagt agcctagaag tagtcaaggc
     6601 ggcgaagtat tcaggcacgt ggccaggaag aagaaaagcc aagacgacga aaacaggtaa
     6661 gagctaagct tcctgcaggg cttttgtcac cggagctgcc agcctgatct ttgagtttta
     6721 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     6781 gagtcggtgc ttttgtcacc ggagtaaagt tttctggtta attgttttag agctagaaat
     6841 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     6901 tttgtcaccg gatgtgtttt ccggtctgat gagtccgtga ggacgaaaca aaagcgtcga
     6961 tcgacaagct cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg
     7021 gttcctatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     7081 tttgtaaaat acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa
     7141 atccagatcc cccgaattac cggcgagctt tgggtacgtc acgtggctcg agcgcgtagt
     7201 cctcggtaat atcgcagaac aaaagtacct gatatcgagt gtacttcaag tcacaccggc
     7261 gagtgccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     7321 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     7381 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     7441 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     7501 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     7561 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     7621 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     7681 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     7741 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     7801 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     7861 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     7921 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     7981 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     8041 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     8101 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     8161 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     8221 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     8281 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     8341 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     8401 cggcaaataa ccctcgagc
//
